RESUMO
Current challenges in the anaerobic bioremediation of benzene are the lack of capable cultures and limited knowledge on the biodegradation pathway. Under methanogenic conditions, benzene may be mineralized by syntrophic interactions between microorganisms, which are poorly understood. The present study developed an optimized formula for anoxic medium to successfully promote the growth of the putative benzene degrader Deltaproteobacterium Hasda-A and enhance the benzene degradation activity of methanogenic enrichment cultures. Within 70| |d of incubation, the benzene degradation activity and relative abundance of Hasda-A in cultures in the new defined medium increased from 0.5 to >3| |mg L-1 d-1 and from 2.5% to >17%, respectively. Together with Hasda-A, we found a strong positive relationship between the abundances of superphylum OD1 bacteria, three methanogens (Methanoregula, Methanolinea, and Methanosaeta) and benzene degradation activity. The syntrophic relationship between these microbial taxa and Hasda-A was then demonstrated in a correlation analysis of longitudinal data. The involvement of methanogenesis in anaerobic benzene mineralization was confirmed by inhibition experiments. The high benzene degradation activity and growth of Hasda-A were quickly recovered in successive dilutions of enrichment cultures, proving the feasibility of using the medium developed in the present study to produce highly capable cultures. The present results will facilitate practical applications in bioremediation and research on the molecular mechanisms underlying benzene activation and syntrophic interactions in benzene mineralization.
Assuntos
Benzeno/metabolismo , Meios de Cultura/química , Deltaproteobacteria/metabolismo , Metano/metabolismo , Methanosarcinales/metabolismo , Anaerobiose , Biodegradação Ambiental , Crescimento Quimioautotrófico , Técnicas de Cocultura , Meios de Cultura/metabolismo , Deltaproteobacteria/crescimento & desenvolvimento , Methanosarcinales/crescimento & desenvolvimentoRESUMO
BACKGROUND: Degradation of acetone by aerobic and nitrate-reducing bacteria can proceed via carboxylation to acetoacetate and subsequent thiolytic cleavage to two acetyl residues. A different strategy was identified in the sulfate-reducing bacterium Desulfococcus biacutus that involves formylation of acetone to 2-hydroxyisobutyryl-CoA. RESULTS: Utilization of short-chain ketones (acetone, butanone, 2-pentanone and 3-pentanone) and isopropanol by the sulfate reducer Desulfosarcina cetonica was investigated by differential proteome analyses and enzyme assays. Two-dimensional protein gel electrophoresis indicated that D. cetonica during growth with acetone expresses enzymes homologous to those described for Desulfococcus biacutus: a thiamine diphosphate (TDP)-requiring enzyme, two subunits of a B12-dependent mutase, and a NAD+-dependent dehydrogenase. Total proteomics of cell-free extracts confirmed these results and identified several additional ketone-inducible proteins. Acetone is activated, most likely mediated by the TDP-dependent enzyme, to a branched-chain CoA-ester, 2-hydroxyisobutyryl-CoA. This compound is linearized to 3-hydroxybutyryl-CoA by a coenzyme B12-dependent mutase followed by oxidation to acetoacetyl-CoA by a dehydrogenase. Proteomic analysis of isopropanol- and butanone-grown cells revealed the expression of a set of enzymes identical to that expressed during growth with acetone. Enzyme assays with cell-free extract of isopropanol- and butanone-grown cells support a B12-dependent isomerization. After growth with 2-pentanone or 3-pentanone, similar protein patterns were observed in cell-free extracts as those found after growth with acetone. CONCLUSIONS: According to these results, butanone and isopropanol, as well as the two pentanone isomers, are degraded by the same enzymes that are used also in acetone degradation. Our results indicate that the degradation of several short-chain ketones appears to be initiated by TDP-dependent formylation in sulfate-reducing bacteria.
Assuntos
2-Propanol/metabolismo , Acetona/metabolismo , Deltaproteobacteria/genética , Deltaproteobacteria/metabolismo , Cetonas/metabolismo , Sulfatos/metabolismo , 2-Propanol/farmacologia , Deltaproteobacteria/efeitos dos fármacos , Deltaproteobacteria/crescimento & desenvolvimento , Cetonas/química , Oxirredução , Proteoma , Proteômica/métodosRESUMO
Biological inorganic carbon fixation proceeds through a number of fundamentally different autotrophic pathways that are defined by specific key enzymatic reactions. Detection of the enzymatic genes in (meta)genomes is widely used to estimate the contribution of individual organisms or communities to primary production. Here we show that the sulfur-reducing anaerobic deltaproteobacterium Desulfurella acetivorans is capable of both acetate oxidation and autotrophic carbon fixation, with the tricarboxylic acid cycle operating either in the oxidative or reductive direction, respectively. Under autotrophic conditions, the enzyme citrate synthase cleaves citrate adenosine triphosphate independently into acetyl coenzyme A and oxaloacetate, a reaction that has been regarded as impossible under physiological conditions. Because this overlooked, energetically efficient carbon fixation pathway lacks key enzymes, it may function unnoticed in many organisms, making bioinformatical predictions difficult, if not impossible.
Assuntos
Processos Autotróficos , Ciclo do Carbono , Dióxido de Carbono/metabolismo , Citrato (si)-Sintase/metabolismo , Deltaproteobacteria/enzimologia , Deltaproteobacteria/crescimento & desenvolvimento , Acetilcoenzima A/metabolismo , Trifosfato de Adenosina/metabolismo , Ácido Cítrico/metabolismo , Ácido Oxaloacético/metabolismoRESUMO
An anaerobic culture (1MN) was enriched with 1-methylnaphthalene as sole source of carbon and electrons and Fe(OH)3 as electron acceptor. 1-Naphthoic acid was produced as a metabolite during growth with 1-methylnaphthalene while 2-naphthoic acid was detected with naphthalene and 2-methylnaphthalene. This indicates that the degradation pathway of 1-methylnaphthalene might differ from naphthalene and 2-methylnaphthalene degradation in sulfate reducers. Terminal restriction fragment length polymorphism and pyrosequencing revealed that the culture is mainly composed of two bacteria related to uncultured Gram-positive Thermoanaerobacteraceae and uncultured gram-negative Desulfobulbaceae. Stable isotope probing showed that a 13C-carbon label from 13C10-naphthalene as growth substrate was mostly incorporated by the Thermoanaerobacteraceae. The presence of putative genes involved in naphthalene degradation in the genome of this organism was confirmed via assembly-based metagenomics and supports that it is the naphthalene-degrading bacterium in the culture. Thermoanaerobacteraceae have previously been detected in oil sludge under thermophilic conditions, but have not been shown to degrade hydrocarbons so far. The second member of the community belongs to the Desulfobulbaceae and has high sequence similarity to uncultured bacteria from contaminated sites including recently proposed groundwater cable bacteria. We suggest that the gram-positive Thermoanaerobacteraceae degrade polycyclic aromatic hydrocarbons while the Desulfobacterales are mainly responsible for Fe(III) reduction.
Assuntos
Deltaproteobacteria/metabolismo , Ferro/metabolismo , Naftalenos/metabolismo , Trifosfato de Adenosina/biossíntese , Anaerobiose , Biodegradação Ambiental , Carbono/farmacologia , Deltaproteobacteria/crescimento & desenvolvimento , Funções Verossimilhança , Metaboloma , Filogenia , Hidrocarbonetos Policíclicos Aromáticos/metabolismo , Polimorfismo de Fragmento de Restrição , RNA Ribossômico 16S/genéticaRESUMO
Anaerobic digestion (AD) is an attractive wastewater treatment technology, leading to the generation of recoverable biofuel (methane). Most industrial AD applications, carry excessive heating costs, however, as AD reactors are commonly operated at mesophilic temperatures while handling waste streams discharged at ambient or cold temperatures. Consequently, low-temperature AD represents a cost-effective strategy for wastewater treatment. The comparative investigation of key microbial groups underpinning laboratory-scale AD bioreactors operated at 37, 15 and 7°C was carried out. Community structure was monitored using 16S rRNA clone libraries, while abundance of the most prominent methanogens was investigated using qPCR. In addition, metaproteomics was employed to access the microbial functions carried out in situ. While δ-Proteobacteria were prevalent at 37°C, their abundance decreased dramatically at lower temperatures with inverse trends observed for Bacteroidetes and Firmicutes. Methanobacteriales and Methanosaeta were predominant at all temperatures investigated while Methanomicrobiales abundance increased at 15°C compared to 37 and 7°C. Changes in operating temperature resulted in the differential expression of proteins involved in methanogenesis, which was found to occur in all bioreactors, as corroborated by bioreactors' performance. This study demonstrated the value of employing a polyphasic approach to address microbial community dynamics and highlighted the functional redundancy of AD microbiomes.
Assuntos
Proteínas Arqueais/metabolismo , Reatores Biológicos , Temperatura Baixa , Euryarchaeota/metabolismo , Methanosarcinales/metabolismo , Proteômica/métodos , Esgotos/microbiologia , Águas Residuárias/microbiologia , Anaerobiose , Proteínas Arqueais/genética , Proteínas de Bactérias/genética , Proteínas de Bactérias/metabolismo , Bacteroidetes/genética , Bacteroidetes/crescimento & desenvolvimento , Bacteroidetes/isolamento & purificação , Biocombustíveis , Deltaproteobacteria/genética , Deltaproteobacteria/crescimento & desenvolvimento , Deltaproteobacteria/isolamento & purificação , Euryarchaeota/genética , Euryarchaeota/crescimento & desenvolvimento , Euryarchaeota/isolamento & purificação , Firmicutes/genética , Firmicutes/crescimento & desenvolvimento , Firmicutes/isolamento & purificação , Methanobacteriales/genética , Methanobacteriales/crescimento & desenvolvimento , Methanobacteriales/isolamento & purificação , Methanosarcinales/genética , Methanosarcinales/crescimento & desenvolvimento , Methanosarcinales/isolamento & purificação , Consórcios Microbianos , RNA Ribossômico 16S/genética , Reação em Cadeia da Polimerase em Tempo Real , TemperaturaRESUMO
Bacterial glycerol ether lipids (alkylglycerols) have received increasing attention during the last decades, notably due to their potential role in cell resistance or adaptation to adverse environmental conditions. Major uncertainties remain, however, regarding the origin, biosynthesis, and modes of formation of these uncommon bacterial lipids. We report here the preponderance of monoalkyl- and dialkylglycerols (1-O-alkyl-, 2-O-alkyl-, and 1,2-O-dialkylglycerols) among the hydrolyzed lipids of the marine mesophilic sulfate-reducing proteobacterium Desulfatibacillum alkenivorans PF2803T grown on n-alkenes (pentadec-1-ene or hexadec-1-ene) as the sole carbon and energy source. Alkylglycerols account for one-third to two-thirds of the total cellular lipids (alkylglycerols plus acylglycerols), depending on the growth substrate, with dialkylglycerols contributing to one-fifth to two-fifths of the total ether lipids. The carbon chain distribution of the lipids of D. alkenivorans also depends on that of the substrate, but the chain length and methyl-branching patterns of fatty acids and monoalkyl- and dialkylglycerols are systematically congruent, supporting the idea of a biosynthetic link between the three classes of compounds. Vinyl ethers (1-alken-1'-yl-glycerols, known as plasmalogens) are not detected among the lipids of strain PF2803T. Cultures grown on different (per)deuterated n-alkene, n-alkanol, and n-fatty acid substrates further demonstrate that saturated alkylglycerols are not formed via the reduction of hypothetic alken-1'-yl intermediates. Our results support an unprecedented biosynthetic pathway to monoalkyl/monoacyl- and dialkylglycerols in anaerobic bacteria and suggest that n-alkyl compounds present in the environment can serve as the substrates for supplying the building blocks of ether phospholipids of heterotrophic bacteria.
Assuntos
Bactérias Anaeróbias/metabolismo , Deltaproteobacteria/metabolismo , Éteres de Glicerila/metabolismo , Metabolismo dos Lipídeos , Sulfatos/metabolismo , Aerobiose , Alcenos/metabolismo , Bactérias Anaeróbias/crescimento & desenvolvimento , Carbono/metabolismo , Deltaproteobacteria/crescimento & desenvolvimento , Metabolismo Energético , Redes e Vias Metabólicas , OxirreduçãoRESUMO
In situ mesocosm experiments using a calcareous sand flat from a coastal area of the island of Mallorca in the Mediterranean Sea were performed in order to study the response of sulfate-reducing bacteria (SRB) to controlled crude oil contamination, or heavy contamination with naphthalene. Changes in the microbial community caused by the contamination were monitored by a combination of comparative sequence analysis of 16S rRNA genes, fluorescence in situ hybridization, cultivation approaches and metabolic activity rates. Our results showed that crude oil and naphthalene negatively influenced the total microbial community as the natural increase in cell numbers due to the seasonal dynamics was attenuated. However, both contaminants enhanced the sulfate reduction rates, as well as the culturability of SRB. Our results suggested the presence of autochthonous deltaproteobacterial SRBs that were able to degrade crude oil or polycyclic aromatic hydrocarbons such as naphthalene in anaerobic sediment layers.
Assuntos
Sedimentos Geológicos/microbiologia , Naftalenos/metabolismo , Petróleo/metabolismo , Bactérias Redutoras de Enxofre/metabolismo , Poluentes Químicos da Água/metabolismo , Sequência de Bases , Vazamento de Resíduos Químicos , Deltaproteobacteria/genética , Deltaproteobacteria/crescimento & desenvolvimento , Deltaproteobacteria/metabolismo , Genes de RNAr , Sedimentos Geológicos/química , Mar Mediterrâneo , Dados de Sequência Molecular , Naftalenos/análise , Petróleo/análise , RNA Ribossômico 16S/metabolismo , Sulfatos/análise , Sulfatos/metabolismo , Bactérias Redutoras de Enxofre/genética , Bactérias Redutoras de Enxofre/crescimento & desenvolvimento , Microbiologia da Água , Poluentes Químicos da Água/análiseRESUMO
Desulfotignum balticum utilizes benzoate coupled to sulfate reduction. Two-dimensional polyacrylamide gel electrophoresis (2D-PAGE) analysis was conducted to detect proteins that increased more after growth on benzoate than on butyrate. A comparison of proteins on 2D gels showed that at least six proteins were expressed. The N-terminal sequences of three proteins exhibited significant identities with the alpha and beta subunits of electron transfer flavoprotein (ETF) from anaerobic aromatic-degraders. By sequence analysis of the fosmid clone insert (37,590 bp) containing the genes encoding the ETF subunits, we identified three genes, whose deduced amino acid sequences showed 58%, 74%, and 62% identity with those of Gmet_2267 (Fe-S oxidoreductase), Gmet_2266 (ETF beta subunit), and Gmet_2265 (ETF alpha subunit) respectively, which exist within the 300-kb genomic island of aromatic-degradation genes from Geobacter metallireducens GS-15. The genes encoding ETF subunits found in this study were upregulated in benzoate utilization.
Assuntos
Benzoatos/farmacologia , Deltaproteobacteria/enzimologia , Deltaproteobacteria/genética , Flavoproteínas Transferidoras de Elétrons/genética , Flavoproteínas Transferidoras de Elétrons/metabolismo , Regulação para Cima/efeitos dos fármacos , Sequência de Aminoácidos , Carbono/química , Clonagem Molecular , DNA Bacteriano/genética , Deltaproteobacteria/efeitos dos fármacos , Deltaproteobacteria/crescimento & desenvolvimento , Eletroforese em Gel Bidimensional , Biblioteca Gênica , Genes Bacterianos/genética , Dados de Sequência Molecular , Oxirredução , Peptídeos/química , Peptídeos/metabolismo , Subunidades Proteicas/genética , Subunidades Proteicas/metabolismo , Reação em Cadeia da Polimerase Via Transcriptase Reversa , Análise de Sequência de DNA , Homologia de Sequência do Ácido NucleicoRESUMO
The lithistid sponge Discodermia dissoluta (family Theonellidae), is found in deep-waters throughout the Caribbean sea and is the source of discodermolide, a natural product with potential anticancer properties, and other secondary metabolites. As with other sponges, large numbers of microbes are harbored in the sponge mesohyl. The microbial population of the sponge mesohyl shows an abundance of large filamentous microbes. Fractionation of the dissociated sponge allowed enrichment of this microbe, which was then identified by analysis of the 16S rRNA genes. Its identity was confirmed through the use of fluorescent in situ hybridization. These studies have allowed the identification of this eubacterial microbe as belonging to the genus Entotheonella.
Assuntos
Deltaproteobacteria/classificação , Poríferos/microbiologia , Simbiose , Animais , DNA Bacteriano , Deltaproteobacteria/genética , Deltaproteobacteria/crescimento & desenvolvimento , Deltaproteobacteria/ultraestrutura , Genes de RNAr , Hibridização in Situ Fluorescente , Microscopia Eletrônica de Transmissão , Dados de Sequência Molecular , Filogenia , Poríferos/ultraestrutura , RNA Ribossômico 16S , Água do Mar , Análise de Sequência de DNARESUMO
The anaerobic biodegradation of benzene, a common constituent of petroleum and one of the least reactive aromatic hydrocarbons, is insufficiently understood with respect to the involved microorganisms and their metabolism. To study these aspects, sulfate-reducing bacteria were enriched with benzene as sole organic substrate using marine sediment as inoculum. Repeated subcultivation yielded a sediment-free enrichment culture constituted of mostly oval-shaped cells and showing benzene-dependent sulfate reduction and growth under strictly anoxic conditions. Amplification and sequencing of 16S rRNA genes from progressively diluted culture samples revealed an abundant phylotype; this was closely related to a clade of Deltaproteobacteria that includes sulfate-reducing bacteria able to degrade naphthalene or other aromatic hydrocarbons. Cell hybridization with two specifically designed 16S rRNA-targeted fluorescent oligonucleotide probes showed that the retrieved phylotype accounted for more than 85% of the cells detectable via DAPI staining (general cell staining) in the enrichment culture. The result suggests that the detected dominant phylotype is the 'candidate species' responsible for the anaerobic degradation of benzene. Quantitative growth experiments revealed complete oxidation of benzene with stoichiometric coupling to the reduction of sulfate to sulfide. Suspensions of benzene-grown cells did not show metabolic activity towards phenol or toluene. This observation suggests that benzene degradation by the enriched sulfate-reducing bacteria does not proceed via anaerobic hydroxylation (mediated through dehydrogenation) to free phenol or methylation to toluene, respectively, which are formerly proposed alternative mechanisms for benzene activation.
Assuntos
Benzeno/metabolismo , Deltaproteobacteria/metabolismo , Sedimentos Geológicos/microbiologia , Sulfatos/metabolismo , Anaerobiose , Benzeno/química , Meios de Cultura , Deltaproteobacteria/crescimento & desenvolvimento , Fenol/química , Fenol/metabolismo , Filogenia , RNA Ribossômico 16S/genética , Sulfatos/química , Tolueno/química , Tolueno/metabolismoRESUMO
Microbial degradation is the only sustainable component of natural attenuation in contaminated groundwater environments, yet its controls, especially in anaerobic aquifers, are still poorly understood. Hence, putative spatial correlations between specific populations of key microbial players and the occurrence of respective degradation processes remain to be unraveled. We therefore characterized microbial community distribution across a high-resolution depth profile of a tar oil-impacted aquifer where benzene, toluene, ethylbenzene, and xylene (BTEX) degradation depends mainly on sulfate reduction. We conducted depth-resolved terminal restriction fragment length polymorphism fingerprinting and quantitative PCR of bacterial 16S rRNA and benzylsuccinate synthase genes (bssA) to quantify the distribution of total microbiota and specific anaerobic toluene degraders. We show that a highly specialized degrader community of microbes related to known deltaproteobacterial iron and sulfate reducers (Geobacter and Desulfocapsa spp.), as well as clostridial fermenters (Sedimentibacter spp.), resides within the biogeochemical gradient zone underneath the highly contaminated plume core. This zone, where BTEX compounds and sulfate--an important electron acceptor--meet, also harbors a surprisingly high abundance of the yet-unidentified anaerobic toluene degraders carrying the previously detected F1-cluster bssA genes (C. Winderl, S. Schaefer, and T. Lueders, Environ. Microbiol. 9:1035-1046, 2007). Our data suggest that this biogeochemical gradient zone is a hot spot of anaerobic toluene degradation. These findings show that the distribution of specific aquifer microbiota and degradation processes in contaminated aquifers are tightly coupled, which may be of value for the assessment and prediction of natural attenuation based on intrinsic aquifer microbiota.
Assuntos
Bactérias Anaeróbias/classificação , Ecossistema , Água Doce/microbiologia , Tolueno/metabolismo , Poluentes Químicos da Água/metabolismo , Abastecimento de Água , Bactérias Anaeróbias/genética , Bactérias Anaeróbias/crescimento & desenvolvimento , Bactérias Anaeróbias/metabolismo , Derivados de Benzeno/metabolismo , Biodegradação Ambiental , Carbono-Carbono Liases , Deltaproteobacteria/classificação , Deltaproteobacteria/genética , Deltaproteobacteria/crescimento & desenvolvimento , Deltaproteobacteria/metabolismo , Água Doce/química , Ferro/metabolismo , Dados de Sequência Molecular , Oxirredução , Filogenia , Reação em Cadeia da Polimerase , Polimorfismo de Fragmento de Restrição , RNA Ribossômico 16S , Análise de Sequência de DNA , Sulfatos/metabolismoAssuntos
Bactérias Anaeróbias/metabolismo , Deltaproteobacteria/crescimento & desenvolvimento , Deltaproteobacteria/metabolismo , Euryarchaeota/metabolismo , Simbiose , Trifosfato de Adenosina/metabolismo , Anaerobiose , Bactérias Anaeróbias/crescimento & desenvolvimento , Deltaproteobacteria/genética , Metabolismo Energético , Euryarchaeota/crescimento & desenvolvimento , Ácidos Graxos , Fermentação , Genoma Bacteriano , Hidrogênio/metabolismo , Oxirredução , Sulfatos/metabolismo , TermodinâmicaRESUMO
The new mesophilic, chemolithoautotrophic, moderately halophilic, sulfate-reducing bacterium strain 11-6 could grow at a NaCl concentration in the medium of 30-230 g/l, with an optimum at 80-100 g/l. Cells were vibrios motile at the early stages of growth. Lactate, pyruvate, malate, fumarate, succinate, propionate, butyrate, crotonate, ethanol, alanine, formate, and H2 + CO2 were used in sulfate reduction. Butyrate was degraded completely, without acetate accumulation. In butyrate-grown cells, a high activity of CO dehydrogenase was detected. Additional growth factors were not required. Autotrophic growth occurred, in the presence of sulfate, on H2 + CO2 or formate without other electron donors. Fermentation of pyruvate and fumarate was possible in the absence of sulfate. Apart from sulfate, sulfite, thiosulfate, and elemental sulfur were able to serve as electron acceptors. The optimal growth temperature was 37 degrees C; the optimum pH was 7.2. Desulfoviridin was not detected. Menaquinone MK-7 was present. The DNA G+C content was 55.2 mol %. Phylogenetically, the bacterium represented a separate branch within the cluster formed by representatives of the family Desulfohalobiaceae in the subclass Deltaproteobacteria. The bacterium was assigned to a new genus and species, Desulfovermiculus halophilus gen. nov., sp. nov. The type strain is 11-6T (= VKM B-2364), isolated from the highly mineralized formation water of an oil field.
Assuntos
Deltaproteobacteria/crescimento & desenvolvimento , Deltaproteobacteria/metabolismo , Petróleo/microbiologia , Bactérias Redutoras de Enxofre/crescimento & desenvolvimento , Bactérias Redutoras de Enxofre/metabolismo , Ácidos Carboxílicos/metabolismo , Deltaproteobacteria/ultraestrutura , Genótipo , Concentração de Íons de Hidrogênio , Oxirredução , Oxirredutases/análise , Filogenia , RNA Ribossômico 16S/genética , Sulfatos/metabolismo , Bactérias Redutoras de Enxofre/ultraestrutura , TemperaturaRESUMO
Phylogenetic screening of 3200 clones from a metagenomic library of Antarctic mesopelagic picoplankton allowed the identification of two bacterial 16S-rDNA-containing clones belonging to the Deltaproteobacteria, DeepAnt-1F12 and DeepAnt-32C6. These clones were very divergent, forming a monophyletic cluster with the environmental sequence GR-WP33-58 that branched at the base of the myxobacteria. Except for the possession of complete rrn operons without associated tRNA genes, DeepAnt-1F12 and DeepAnt-32C6 were very different in gene content and organization. Gene density was much higher in DeepAnt-32C6, whereas nearly one-third of DeepAnt-1F12 corresponded to intergenic regions. Many of the predicted genes encoded by these metagenomic clones were informational (i.e. involved in replication, transcription, translation and related processes). Despite this, a few putative cases of horizontal gene transfer were detected, including a transposase. DeepAnt-1F12 contained one putative gene encoding a long cysteine-rich protein, probably membrane-bound and Ca2+-binding, with only eukaryotic homologues. DeepAnt-32C6 carried some predicted genes involved in metabolic pathways that suggested this organism may be anaerobic and able to ferment and to degrade complex compounds extracellularly.
Assuntos
Deltaproteobacteria/genética , Regiões Antárticas , DNA Bacteriano/análise , DNA Ribossômico/análise , Deltaproteobacteria/enzimologia , Deltaproteobacteria/crescimento & desenvolvimento , Genoma Bacteriano , Dados de Sequência Molecular , Plâncton , RNA Ribossômico 16S/análise , RNA Ribossômico 16S/genéticaRESUMO
Iron-reducing bacteria can transfer electrons to ferric iron oxides which are barely soluble at neutral pH, and electron-shuttling compounds or chelators are discussed to be involved in this process. Experiments using semipermeable membranes for separation of ferric iron-reducing bacteria from ferric iron oxides do not provide conclusive results in this respect. Here, we used ferrihydrite embedded in 1% agar to check for electron-shuttling compounds in pure and in enrichment cultures. Geobacter sulfurreducens reduced spatially distant ferrihydrite only in the presence of anthraquinone-2,6-disulfonate, a small molecule known to shuttle electrons between the bacterial cell and ferrihydrite. However, indications for the production and excretion of electron-shuttling compounds or chelators were found in ferrihydrite-containing agar dilution cultures that were inoculated with ferric iron-reducing enrichment cultures.
Assuntos
Deltaproteobacteria/metabolismo , Compostos Férricos/metabolismo , Antraquinonas/metabolismo , Grupo dos Citocromos c/química , Deltaproteobacteria/crescimento & desenvolvimento , Transporte de Elétrons , Ferritinas/metabolismo , Ferro/metabolismo , OxirreduçãoRESUMO
In vitro assays demonstrated that toluene-grown cells of Geobacter metallireducens catalyzed the addition of toluene to fumarate to form benzylsuccinate under anaerobic conditions. The specific in vitro rate of benzylsuccinate formation was ca. 45% of the specific in vivo rate of toluene consumption. In addition, bssA and bssB, which code for the alpha and beta subunits of benzylsuccinate synthase (BSS), respectively, were found to have sequences in G. metallireducens similar to the only sequences heretofore available (for three denitrifying strains). This is the first report of the presence of BSS in a ferric iron-reducing bacterium; BSS activity has previously been reported in denitrifying, sulfate-reducing, and anoxygenic phototrophic toluene degraders, as well as in a highly enriched methanogenic, toluene-degrading culture.
Assuntos
Carbono-Carbono Liases/metabolismo , Deltaproteobacteria/metabolismo , Ferro/metabolismo , Tolueno/metabolismo , Sequência de Aminoácidos , Anaerobiose , Biodegradação Ambiental , Carbono-Carbono Liases/genética , Deltaproteobacteria/genética , Deltaproteobacteria/crescimento & desenvolvimento , Cinética , Dados de Sequência Molecular , Oxirredução , Subunidades Proteicas/genética , Alinhamento de Sequência , Succinatos/metabolismoRESUMO
AIMS: The aim of this work was to develop a highly selective method of detecting sulphate-reducing bacteria (SRB) in crude oil. METHODS: A pair of PCR primers was designed based on an alignment of the nucleotide sequence of the 16S rRNA genes from the Desulfovibrionaceae family. DNA extraction from crude oil was performed by the method using zirconia beads and a stool kit. RESULTS: The PCR specifically detected Desulfovibrio and Desulfomicrobium in a sediment sample. When nucleic acids extracted directly from crude oil were used for the PCR, 16S rRNA genes of Desulfovibrio and Thermodesulforhabdus norvegicus were detected. IMPACT OF STUDY: A simple direct method for detection of the SRB in crude oil using PCR was established.
Assuntos
Petróleo/microbiologia , Bactérias Redutoras de Enxofre/isolamento & purificação , Primers do DNA , DNA Bacteriano/análise , DNA Bacteriano/isolamento & purificação , DNA Ribossômico/análise , Deltaproteobacteria/classificação , Deltaproteobacteria/crescimento & desenvolvimento , Deltaproteobacteria/isolamento & purificação , Desulfovibrio/classificação , Desulfovibrio/genética , Desulfovibrio/isolamento & purificação , Sedimentos Geológicos/microbiologia , Dados de Sequência Molecular , Filogenia , Reação em Cadeia da Polimerase , RNA Ribossômico 16S/genética , Análise de Sequência de DNA , Bactérias Redutoras de Enxofre/classificação , Bactérias Redutoras de Enxofre/genéticaRESUMO
The dissimilatory iron-reducing bacterium Geobacter metallireducens was found to require iron at a concentration in excess of 50 microM for continuous cultivation on nitrate. Growth yield (approximately 3-fold), cytochrome c content (approximately 7-fold), and nitrate (approximately 4.5-fold) and nitrite (approximately 70-fold) reductase activities were all increased significantly when the growth medium was amended with 500 microM iron.
Assuntos
Deltaproteobacteria/metabolismo , Ferro/metabolismo , Nitratos/metabolismo , Meios de Cultura , Grupo dos Citocromos c/metabolismo , Deltaproteobacteria/crescimento & desenvolvimento , Regulação Bacteriana da Expressão Gênica , Nitrato Redutase , Nitrato Redutases/metabolismo , Nitrito Redutases/metabolismo , OxirreduçãoRESUMO
A dissimilatory sulfite reductase (DSR) was purified from the anaerobic, taurine-degrading bacterium Bilophila wadsworthia RZATAU to apparent homogeneity. The enzyme is involved in energy conservation by reducing sulfite, which is formed during the degradation of taurine as an electron acceptor, to sulfide. According to its UV-visible absorption spectrum with maxima at 392, 410, 583, and 630 nm, the enzyme belongs to the desulfoviridin type of DSRs. The sulfite reductase was isolated as an alpha2beta)gamma(n) (n > or = 2) multimer with a native size of 285 kDa as determined by gel filtration. We have sequenced the genes encoding the alpha and beta subunits (dsrA and dsrB, respectively), which probably constitute one operon. dsrA and dsrB encode polypeptides of 49 (alpha) and 54 kDa (beta) which show significant similarities to the homologous subunits of other DSRs. The dsrB gene product of B. wadsworthia is apparently a fusion protein of dsrB and dsrD. This indicates a possible functional role of DsrD in DSR function because of its presence as a fusion protein as an integral part of the DSR holoenzyme in B. wadsworthia. A phylogenetic analysis using the available Dsr sequences revealed that B. wadsworthia grouped with its closest 16S rDNA relative Desulfovibrio desulfuricans Essex 6.
Assuntos
Deltaproteobacteria/enzimologia , Oxirredutases atuantes sobre Doadores de Grupo Enxofre/genética , Oxirredutases atuantes sobre Doadores de Grupo Enxofre/metabolismo , Taurina/metabolismo , Sequência de Aminoácidos , Anaerobiose , Deltaproteobacteria/genética , Deltaproteobacteria/crescimento & desenvolvimento , Eletroforese em Gel de Poliacrilamida , Dados de Sequência Molecular , Oxirredutases/metabolismo , Oxirredutases atuantes sobre Doadores de Grupo Enxofre/isolamento & purificação , Filogenia , Análise de Sequência de DNA , Sulfatos/metabolismo , Sulfitos/metabolismoRESUMO
Nitrate reduction in the dissimilatory iron-reducing bacterium Geobacter metallireducens was investigated. Nitrate reductase and nitrite reductase activities in nitrate-grown cells were detected only in the membrane fraction. The apparent K(m )values for nitrate and nitrite were determined to be 32 and 10 microM, respectively. Growth on nitrate was not inhibited by either tungstate or molybdate at concentrations of 1 mM or less, but was inhibited by both at 10 and 20 mM. Nitrate and nitrite reductase activity in the membrane fraction was not, however, affected by dialysis with 20 mM tungstate. An enzyme complex that exhibited both nitrate and nitrite reductase activity was solubilized from membrane fractions with CHAPS and was partially purified by preparative gel electrophoresis. It was found to be composed of four different polypeptides with molecular masses of 62, 52, 36, and 16 kDa. The 62-kDa polypeptide [a low-midpoint potential (-207 mV), multiheme cytochrome c] exhibited nitrite reductase activity under denaturing conditions. No molybdenum was detected in the complex by plasma-emission mass spectrometry.