RESUMO
OBJECTIVE: The aim of this study was to investigate the signal intensity characteristics of highly invasive and highly metastasizing transplanted human squamous cell carcinoma using ultra-small super-paramagnetic iron oxide (USPIO)-enhanced MRI and to correlate them with USPIO distribution to tumour components revealed by histological examination. METHODS: 13 nude mice with transplanted human squamous cell carcinoma in the oral cavity were imaged before and 24 hours after intravenous administration of USPIO. The difference in signal intensity between pre-contrast and post-contrast MR images was visually evaluated. For quantitative analysis, signal intensity within a region of interest was measured. Histological findings were correlated with MR findings. The approximate USPIO concentration was evaluated using USPIO phantoms. RESULTS: Seven tumours had an area showing signal intensity increase on post-contrast T1 weighted images. Histopathologically, six of those tumours contained a small amount of iron particles in the stroma. The USPIO concentration was presumed low. Two tumours had an area showing signal intensity decrease on post-contrast T1 and T2 weighted images. The areas had a large amount of iron particles in the stroma and the USPIO concentration was presumed high. There was a minimal amount of iron particles in tumour parenchymal cells. CONCLUSIONS: The amount of USPIO accumulation into tumour stroma was considered to affect MR signal intensity. A small amount increases T1 weighted signal intensity, whereas a large amount decreases T1 and T2 weighted intensity. The USPIO accumulation into the tumour parenchyma was not thought to affect MR signal intensity.
Assuntos
Carcinoma de Células Escamosas/diagnóstico por imagem , Meios de Contraste , Dextranos , Aumento da Imagem/métodos , Imageamento por Ressonância Magnética/métodos , Nanopartículas de Magnetita , Transplante de Neoplasias/diagnóstico por imagem , Neoplasias da Língua/diagnóstico por imagem , Animais , Carcinoma de Células Escamosas/patologia , Carcinoma de Células Escamosas/secundário , Técnicas de Cultura de Células , Linhagem Celular Tumoral , Tecido Conjuntivo/diagnóstico por imagem , Tecido Conjuntivo/patologia , Meios de Contraste/administração & dosagem , Meios de Contraste/normas , Dextranos/administração & dosagem , Dextranos/normas , Humanos , Processamento de Imagem Assistida por Computador/métodos , Injeções Intravenosas , Nanopartículas de Magnetita/administração & dosagem , Nanopartículas de Magnetita/normas , Camundongos , Camundongos Nus , Invasividade Neoplásica , Transplante de Neoplasias/patologia , Imagens de Fantasmas/normas , Radiografia , Padrões de Referência , Neoplasias da Língua/patologiaRESUMO
An analysis of biological properties of blood substitutes has shown the expedience of wider clinical use instead of polyglucin of such substitutes as polyglucol, polifer, rondex, neorondex, polyoxidin, volecam; instead of rheopolyglucin--the rheopolyglucin with glucose, rheogluman and rheoglusol prepared for clinical tests; instead of haemodesin--haemodesin-H, neohaemodesin, gluconeodesin; instead of protein hydrolysates--polyamine and purified from peptides infusamine, hydromine, amicin; instead of 0.9% solution of sodium chloride--disol, quintasol, lactasol, mafusol. They have polyfunctional properties and considerably increase the effectiveness of the infusion--transfusion therapy.