Antioxidant defences of Spironucleus vortens: Glutathione is the major non-protein thiol.

The aerotolerant hydrogenosome-containing piscine diplomonad, Spironucleus vortens, is able to withstand high fluctuations in O2 tensions during its life cycle. In the current study, we further investigated the O2 scavenging and antioxidant defence mechanisms which facilitate the survival of S. vortens under such oxidizing conditions. Closed O2 electrode measurements revealed that the S. vortens ATCC 50386 strain was more O2 tolerant than a freshly isolated S. vortens intestinal strain (Sv1). In contrast to the related human diplomonad, Giardia intestinalis, RP-HPLC revealed the major non-protein thiols of S. vortens to be glutathione (GSH, 776 nmol/107 cells) with cysteine and H2S as minor peaks. Furthermore, antioxidant proteins of S. vortens were assayed enzymatically and revealed that S. vortens possesses superoxide dismutase and NADH oxidase (883 and 37.5nmol/min/mg protein, respectively), but like G. intestinalis, lacks catalase and peroxidase activities. Autofluorescence of NAD(P)H and FAD alongside the fluorescence of the GSH-adduct in monochlorobimane-treated live organisms allowed the monitoring of redox balances before and after treatment with inhibitors, metronidazole and auranofin. H2O2 was emitted into the exterior of S. vortens at a rate of 2.85 pmol/min/106 cells. Metronidazole and auranofin led to depletion of S. vortens intracellular NAD(P)H pools and an increase in H2O2 release with concomitant oxidation of GSH, respectively. Garlic-derived compounds completely inhibited O2 consumption by S. vortens (ajoene oil), or significantly depleted the intracellular GSH pool of the organism (allyl alcohol and DADS). Hence, antioxidant defence mechanisms of S. vortens may provide novel targets for parasite chemotherapy.

Host specificity of cloned Spironucleus sp. originating from the European hamster.

In vivo experiments with a clone of the intestinal flagellate Spironucleus sp. originating from the laboratory European hamster (Cricetus cricetus), and the comparison with a spontaneous infection from laboratory bred European hamsters suggest high specificity of this clone for the homologous host. Only the Syrian golden hamster (Mesocricetus auratus) could be infected experimentally, though the mean intensity of infection was lower. Other heterologous recipients, mice and rats of one outbred and one inbred strain each, could not be infected. Even immunodeficient mice (athymic and C.B-17-scid) remained uninfected after inoculation of 5 x 10(5) cysts per mouse. This is the second Spironucleus clone, after the rat isolate (Schagemann et al., 1990), with a high level of host specificity suggesting heterogeneity within the genus Spironucleus.