RESUMO
Corynebacterium pseudotuberculosis is the causative agent of caseous lymphadenitis, a debilitating chronic disease of sheep and goats. Little is known about the buck's reproductive pathophysiology with respect to inoculation with Corynebacterium pseudotuberculois and its immunogen mycolic acid extract. Therefore, this present study was designed to determine the concentration of testosterone hormone, pro-inflammatory cytokines, and semen quality of the experimental animals. A total of 12 bucks, divided into groups 1, 2, and 3 (Negative control group, Positive control group and Mycolic acid group respectively), were enrolled in this study. Following inoculation, all goats were observed for clinical responses and monitored for 60 days post-challenge and were then sacrificed. Blood samples were collected via the jugular once before inoculation and on a weekly basis post-challenge. Semen samples were collected 2 weeks post-challenge and prior to the sacrifice of the experimental animals. During the post inoculation period of 60 days, the concentration of testosterone hormone for group 2 was increased significantly (p < 0.05) in weeks 5, 6, and 9 but decreased in weeks 2 and 7 post inoculation. In group 3, the mean concentration of testosterone was increased significantly (p < 0.05) in weeks 5, 6, 7, and 9 post inoculation but decreased in week 2. The concentration of interleukin 6 (IL 6) in treated group 2 did not show any significant difference (p > 0.05) but increased significantly (p < 0.05) in week 2 post inoculation in group 3. For concentration of interleukin 1ß (IL1ß) in both treated groups 2 and 3 showed significant difference (p < 0.05) in weeks 2 and 3 post inoculation. The tumor necrosis factor-alpha (TNF-α) concentration in both treated groups 2 and 3 did not show any significant difference (p > 0.05) as compared to group 1. The concentration of interferon-γ (IFNγ) significantly increased (p < 0.05) for group 2 for weeks 2, 3, 4, and 5 where else for group 3 was not in significant difference (p > 0.05) compared to group 1. Both group 2 and group 3 showed a reduction in semen qualities as compared to group 1, but the severity was more intense in group 2 if compared to group 3. In conclusion, therefore, the present study concluded that the mycolic acid group revealed significant responses of testosterone hormone concentration, semen quality, and its related pro-inflammatory cytokines in bucks following infection but the severity lesser compared to Corynebacterium pseudotuberculosis group.
Assuntos
Corynebacterium pseudotuberculosis/fisiologia , Citocinas/sangue , Cabras/fisiologia , Análise do Sêmen/veterinária , Testosterona/sangue , Animais , Infecções por Corynebacterium/imunologia , Infecções por Corynebacterium/microbiologia , Infecções por Corynebacterium/fisiopatologia , Infecções por Corynebacterium/veterinária , Doenças das Cabras/imunologia , Doenças das Cabras/microbiologia , Doenças das Cabras/fisiopatologia , Cabras/imunologia , Masculino , Ácidos Micólicos/metabolismoRESUMO
The relationships between rectal temperatures and physiological and cellular responses to heat stress can improve the productivity of Saanen goats in tropical environments. In this context, this study evaluated the physiological responses and gene expression of heat shock proteins (HSP60, 70, and 90) and genes related to apoptosis (Bax, Bcl-2, and p53) of Saanen goats subjected to acute heat stress. Ten health Saanen goats were exposed to solar radiation during 3 consecutive days. The expression of HSP60, HSP70, HSP90, Bax, Bcl-2, and p53 genes in blood leukocytes, rectal and superficial temperatures, respiratory frequency, cortisol, triiodothyronine, and thyroxine was measured at 06:00, 13:00, and 18:00 h. In vitro, blood leukocytes were subjected to 38 °C and 40 °C for 3 h to measure the expression of the same target genes. The temperature humidity index, measured from 12:00 to 15:00, was greater than 80 and black globe temperatures were greater at 40 °C, indicating the intensity of the solar radiation. Although the solar radiation caused acute heat stress, increased cortisol release, and the expression of HSP60 and 70 in dry Saanen goats, the increased respiratory frequency and decreased T4 and T3 restored the homeothermy of the experimental goats. In vitro, the 40 °C increased the expression of p53 (pro-apoptotic protein), Bcl-2 (anti-apoptotic protein), HSP60, HSP70, and HSP90, suggesting that these genes have protective functions. However, further studies are necessary to understand the physiological and cellular responses to heat stress.
Assuntos
Doenças das Cabras/fisiopatologia , Cabras/fisiologia , Transtornos de Estresse por Calor/fisiopatologia , Transtornos de Estresse por Calor/veterinária , Animais , Temperatura Corporal , Feminino , Doenças das Cabras/sangue , Doenças das Cabras/genética , Transtornos de Estresse por Calor/sangue , Transtornos de Estresse por Calor/genética , Proteínas de Choque Térmico/genética , Umidade , Hidrocortisona/sangue , Leucócitos/metabolismo , Proteínas Proto-Oncogênicas c-bcl-2/genética , Temperatura , Tiroxina/sangue , Tri-Iodotironina/sangue , Proteína Supressora de Tumor p53/genéticaRESUMO
Hydrometra is characterized by the accumulation of fluid within the uterus due to the persistence of corpus luteum. The diagnosis of this disorder occurs with an ultrasonic exam. This study evaluated uterine drainage and fertility rates in goats after the use of d-cloprostenol in association or not with Gonadotropin-releasing hormone (GnRH) treatment. Twenty Saanen goats, diagnosed with hydrometra, received three 37.5-µg doses of d-cloprostenol laterovulvarly at 10-day intervals. On D5, the goats were assigned into two groups receiving 1 mL of GnRH or saline solution intramuscularly. Ultrasonography (US) was performed from D0 to D25. An US approach was used to rank hydrometra in scores. The pregnancy rate was assessed 45 and 90 days after the end of treatment. The uterine fluid was totally drained after the first and second administration of d-cloprostenol in 50% and 95% of the goats, respectively. In one female, full emptying of the uterus occurred only after D20. US performed at 45 and 90 days after the end of treatment indicated there was a pregnancy rate of 45.0% and 55.0%, respectively. Fertility did not differ between the GnRH-treated and control goats. Those goats not pregnant at 45 days had a follicular cyst, hydrosalpinx or hydrometra. At 90 days, no change was observed in the hydrosalpinx, and four goats had hydrometra. The use of three doses of d-cloprostenol 10 days apart was efficient for induction of draining the contents of the uterus, resulting in a relatively acceptable pregnancy rate. This treatment associated with the US approach can be important when applied in the field.
Assuntos
Cloprostenol/uso terapêutico , Doenças das Cabras/tratamento farmacológico , Ultrassonografia/veterinária , Doenças Uterinas/veterinária , Animais , Dinoprosta , Sincronização do Estro , Feminino , Doenças das Cabras/diagnóstico por imagem , Doenças das Cabras/fisiopatologia , Cabras , Hormônio Liberador de Gonadotropina , Inseminação Artificial , Gravidez , Taxa de Gravidez , Reprodução , Estações do Ano , Ultrassom , Ultrassonografia/métodos , Doenças Uterinas/diagnóstico por imagem , Doenças Uterinas/tratamento farmacológico , Doenças Uterinas/fisiopatologiaRESUMO
Peste des petits ruminants (PPR), an economically important viral transboundary disease of small ruminants is not only prevalent in Pakistan but also in other countries where people rely on agriculture and animal products. The present study was aimed at describing the pathology and antigen localization in natural PPR infections in local (Kajli sheep; Beetal goats) as well as imported small ruminant breeds (Dorper sheep; Australian Boer goat). Morbidity and mortality rates were significantly (P < 0.001) higher in indigenous Kajli sheep (75.37 and 32.80%) and Beetal goats (81.10 and 37.24%) as compared to Dorper sheep (6.99 and 1.48%) and Australian Boer goat (5.01 and 2.23%). Affected animals exhibited high fever, severe diarrhea, abdominal pain, respiratory distress and nodular lesions on lips and nostrils. Thick mucous discharge was oozing out from nostrils. On necropsy, lungs were congested and pneumonic, with nodular and cystic appearance. Intestines were hemorrhagic with zebra stripping. Characteristic histopathological lesions of PPR were noted in intestines, lymphoid organs and lungs. In GI tract, stunting and blunting of villi, necrotic enteritis, and infiltration of mononuclear cells in duodenum, jejunum and ileum. Small intestines exhibited diffuse edema of the submucosa along with proliferation of fibrocytes leading to thickened submucosa which has not been reported previously. Lymphoid organs showed partial to complete destruction of lymphoid follicles. Lesions of the respiratory tract included depictive of bronchopneumonia, severe congestion of trachea and apical lobe of lungs with deposition of fibrinous materials. Histopathological lesions of respiratory tract were severe and characteristic of broncho-interstitial pneumonia, bronchopneumonia, interstitial pneumonia and fibrinous pneumonia. The alveoli were filled with edematous fluid mixed with fibrinous exudate, numerous alveolar macrophages, mononuclear cells along with thickened interalveolar septa and presence of intranuclear eosinophilic inclusion bodies. One-Step RT-PCR using NP3 and NP4 primers confirmed a PPR virus of 352 bp size in spleen, lungs and mesenteric and brachial lymph node samples. It was concluded that morbidity and mortality due to PPR were significantly higher in indigenous breeds of sheep and goat as compared to imported sheep and goat breeds. PPR has rendered various lesions in GI and respiratory tract which are characteristic in nature for the diagnosis of the disease under field condition.
Assuntos
Doenças das Cabras/patologia , Doenças das Cabras/fisiopatologia , Peste dos Pequenos Ruminantes/patologia , Peste dos Pequenos Ruminantes/fisiopatologia , Vírus da Peste dos Pequenos Ruminantes/patogenicidade , Doenças dos Ovinos/patologia , Doenças dos Ovinos/fisiopatologia , Animais , Doenças das Cabras/diagnóstico , Doenças das Cabras/epidemiologia , Cabras , Intestinos/patologia , Intestinos/virologia , Rim/patologia , Rim/virologia , Fígado/patologia , Fígado/virologia , Pulmão/patologia , Pulmão/virologia , Paquistão/epidemiologia , Peste dos Pequenos Ruminantes/diagnóstico , Peste dos Pequenos Ruminantes/epidemiologia , Vírus da Peste dos Pequenos Ruminantes/genética , Prevalência , Sistema Respiratório/patologia , Sistema Respiratório/virologia , Ruminantes , Ovinos , Doenças dos Ovinos/diagnóstico , Doenças dos Ovinos/epidemiologia , Carneiro Doméstico , Baço/patologia , Baço/virologiaRESUMO
BACKGROUND: Visceral hypersensitivity (VH) is a common condition in many gastrointestinal disorders such as inflammatory bowel diseases (IBDs) in human and animals. Most studies often induce Crohn's disease/colitis to investigate VH in small experimental animals. Although farm animals commonly suffer from IBDs, their VH has not been investigated so far. Because goats can suffer from Johne's disease, a naturally occurring Crohn's-like disease, they may be suitable to be used for studying the mechanism underlying VH in common intestinal disorders of large animals. In the present study, 60 healthy goats of either sex were equally divided into a 2, 4, 6-trinitrobenzenesulfonic acid (TNBS) group and saline group. A volume of 1.2 ml of TNBS-ethanol solution (30 mg TNBS in 40 % ethanol) or an equal volume of isotonic saline was injected into the wall of the terminal ileum through laparotomy. The severity of the developing ileitis was determined according to macro- and microscopic pathologic scores and the levels of myeloperoxidase, interleukin-1ß, interleukin-6 and tumor necrosis factor-α, and VH was evaluated with visceromotor responses (VMR) to colorectal distension on days 3, 7, 14, 21 and 28. VMRs were assessed with a continuous ramp distention mode with 6 s for each pressure (20, 40, 60, 80 and 100 mmHg). RESULTS: Compared to the saline group, the TNBS-treated goats showed apparent transmural pathological changes and a significant increase (P < 0.05) in macroscopic and microscopic change scores, and levels of myeloperoxidase, interleukin-1ß, interleukin-6 and tumor necrosis factor-α in the ileum, and VMR to colorectal distension. The goats exhibited apparent ileitis at days 3 to 21, and VH at days 7 to 28 following TNBS treatment. CONCLUSION: This experiment successfully established a reproducible ileitis and VH with administration of TNBS-ethanol solution in the ileal wall of goats. This model is useful for studying the pathogenesis of the IBD and the mechanism underlying VH, and for evaluating the efficacy of new therapeutic regimens.
Assuntos
Modelos Animais de Doenças , Doenças das Cabras/fisiopatologia , Hipersensibilidade/veterinária , Ileíte/veterinária , Doenças Inflamatórias Intestinais/veterinária , Animais , Citocinas/análise , Etanol , Feminino , Cabras , Hipersensibilidade/etiologia , Ileíte/induzido quimicamente , Ileíte/complicações , Íleo/metabolismo , Íleo/fisiopatologia , Doenças Inflamatórias Intestinais/fisiopatologia , Masculino , Peroxidase/metabolismo , Ácido Trinitrobenzenossulfônico , Vísceras/patologiaRESUMO
The present study aimed to evaluate the effects of Trypanosoma vivax infection on the shape of the lactation curve and the milk quality of dairy goats experimentally infected with T. vivax. In total, twenty Saanen goats, aged 26-30 months and the same number of calving (two calvings), were divided into two experimental groups: an infected group, consisting of ten goats intravenously infected with 0.5 ml of blood containing approximately 1.25 × 10(5) trypomastigotes of T. vivax and ten uninfected animals as the control group. Clinical tests and hematocrit, parasitemia, and serum biochemistry evaluations were performed on all of the goats. Milk production was measured daily for 152 days by hand milking the goats and weighing the milk. Every seven days, physiochemical analyses were performed to evaluate the milk. Wood's nonlinear model was used to analyze the lactation curve parameters. The infected goats had high levels of parasitemia and hyperthermia, significantly reduced hematocrit, serum total protein, albumin, and glucose levels and increased cholesterol and urea concentrations. Wood's model indicated that the milk production of goats in the infected group declined sharply over a short period of time and produced a flattened yield curve and significant difference (P < 0.05) in the rate of increase of peak milk production, rate of decrease of milk production after the peak, day of peak milk production, and maximum peak milk production compared with that of the control group. Trypanosomiasis also affected the persistency of lactation, which was significantly reduced in goats in the infected group. In addition, the physico-chemical properties of the milk, including the fat content, defatted dry extracts (DDE) and protein content, decreased significantly (P < 0.05) in the goats in the infected group compared with those in the control group. The T. vivax-infected goats showed reduction in milk production, persistence of lactation, and fat levels, the defatted dry extract (DDE) content, and protein, changing the quality of milk.
Assuntos
Doenças das Cabras/fisiopatologia , Transtornos da Lactação/veterinária , Leite/normas , Trypanosoma vivax , Tripanossomíase Africana/veterinária , Ração Animal , Animais , Análise Química do Sangue/veterinária , Proteínas Sanguíneas/metabolismo , Temperatura Corporal , Colesterol/sangue , Ingestão de Alimentos , Feminino , Doenças das Cabras/parasitologia , Cabras , Hematócrito/veterinária , Transtornos da Lactação/parasitologia , Leite/química , Parasitemia/veterinária , Distribuição Aleatória , Tripanossomíase Africana/fisiopatologia , Ureia/sangueRESUMO
A disease with clinical manifestations of thickening and alopecia of the skin over the lower limbs, around the eyes, face, and nose, thickening and shrinkage of the scrotum, and presence of white granular cysts in the sclero-conjunctiva in goats in Kerman Province, were reported to the Pathology Department of Shiraz Veterinary School. Primary histopathological studies demonstrated an outbreak of caprine besnoitiosis in this region. To study the histopathological and ultrastructural features of the disease, samples were collected from various organs of the suspected slaughtered goats for further investigations. In histopathological studies, dermis and subcutaneous fascia covering lower portion of the limbs, skin over frontal sinus, ear tips, scrotum, eye lids as well as the eye's sclera, epididymal and testicular parenchyma, and their tunics were severely infected with Besnoitia cysts. Tongue, pharynx, prepuce and penis, deeper striated muscles, subcutaneous bone matrices, abomasum, esophagus, subcutaneous tendons, and periosteal surfaces of the limb bones showed lower rates of infection. Except the vagina and vestibule, no cyst was observed in other female urogenital organs, the central nervous system, intestines, heart, liver, spleen, and different lymph nodes. The host reaction to the cysts was variable, ranging from the absence of inflammatory cells around intact normal cysts up to infiltration of macrophages, lymphocytes, plasma cells, eosinophils, fibroblasts, and connective tissues around the degenerated cysts. Ultrastructural studies showed this coccidian parasite belonged to eukaryotic protozoa, and the cystic form had the typical feature of the Besnoitia spp. of the apicomplexa. This study showed that the organism demonstrated ultrastructurally minor differences with other Besnoitia species infecting other animal species.
Assuntos
Coccidiose/veterinária , Doenças das Cabras/parasitologia , Sarcocystidae/isolamento & purificação , Sarcocystidae/ultraestrutura , Estruturas Animais/parasitologia , Estruturas Animais/patologia , Animais , Coccidiose/epidemiologia , Coccidiose/patologia , Coccidiose/fisiopatologia , Feminino , Doenças das Cabras/epidemiologia , Doenças das Cabras/patologia , Doenças das Cabras/fisiopatologia , Cabras , Irã (Geográfico)/epidemiologia , MasculinoRESUMO
The aim of this study was to investigate mineral metabolism and immune response in dairy goats following intramammary inoculation with varying doses of Staphylococcus aureus. Blood samples were collected at 0, 2, 4, 8, 12, 24, 48, and 72 h after intramammary inoculation. Lowered plasma Fe concentrations were observed from 12 to 24 h postinoculation in groups SAA (Staph. aureus at 10(4) cfu, n = 5) and SAB (Staph. aureus at 10(8) cfu, n = 5). Plasma Cu concentrations increased in group SAB 2 h after inoculation and maintained greater concentrations until the end of the experiment compared with the control group (phosphate-buffered saline, n = 5). Increased plasma Zn concentrations in group SAB were observed 48 h after inoculation, and the concentration was still greater 72 h after inoculation compared with the control group. Greater plasma Mg concentrations were detected in groups SAA and SAB compared with the control group at all timepoints after inoculation. Plasma Mg concentrations were generally greater in group SAA than in group SAB through 72 h (except at 2 h). Plasma tumor necrosis factor-alpha concentrations were unchanged following intramammary inoculation with Staph. aureus throughout the study. Plasma IL-6 concentrations in groups SAA and SAB increased gradually compared with the control group and peaked at 48 h after inoculation. In group SAB, serum cortisol concentrations started to increase from 8 h postinoculation and peaked at 12 h postinoculation. In conclusion, increasing the inoculum dose does not induce more rapid proinflammatory cytokine responses, whereas the data indicate that mineral metabolic alterations occur during the course of Staph. aureus mastitis in the goat.
Assuntos
Indústria de Laticínios , Doenças das Cabras/fisiopatologia , Cabras/microbiologia , Mastite/veterinária , Infecções Estafilocócicas/veterinária , Staphylococcus aureus/crescimento & desenvolvimento , Animais , Feminino , Doenças das Cabras/sangue , Hidrocortisona/sangue , Interleucina-6/sangue , Glândulas Mamárias Animais/microbiologia , Mastite/fisiopatologia , Metais/sangue , Infecções Estafilocócicas/sangue , Infecções Estafilocócicas/fisiopatologia , Staphylococcus aureus/imunologia , Fatores de Tempo , Fator de Necrose Tumoral alfa/sangueRESUMO
The present work investigates some clinical, endocrinological, biochemical and haematological variables in desert sheep and goats stressed in the course of individual road transportation, and the influence thereon of pretreatment with an established anti-stressor drug, xylazine HCl, and a test compound, sodium betaine (trimethylglycine). Road transportation for 2h resulted in variable and statistically insignificant increases in heart, pulse and respiratory rates in both control and experimental animals. Transportation stress significantly increased the concentrations of plasma cortisol, and glucose, and decreased that of magnesium. The endogenous thiocyanate concentration was unaffected. The stress also insignificantly decreased the haematocrit (PCV), and the number of lymphocytes, and increased the concentration of haemoglobin. Pretreatment of sheep and goats with xylazine at a single dose of 0.01 mg/kg by the intravenous route significantly ameliorated the effects induced by the stressful stimulus. The effects of pretreatment of the two species with sodium betaine (10 mg/kg) produced variable and insignificant effects.
Assuntos
Agonistas alfa-Adrenérgicos/farmacologia , Betaína/farmacologia , Doenças das Cabras/prevenção & controle , Doenças dos Ovinos/prevenção & controle , Estresse Fisiológico/veterinária , Xilazina/farmacologia , Animais , Glicemia/metabolismo , Doenças das Cabras/etiologia , Doenças das Cabras/metabolismo , Doenças das Cabras/fisiopatologia , Cabras , Frequência Cardíaca/efeitos dos fármacos , Hematócrito/veterinária , Hemoglobinas/metabolismo , Hidrocortisona/sangue , Contagem de Linfócitos/veterinária , Masculino , Pulso Arterial/veterinária , Respiração/efeitos dos fármacos , Ovinos , Doenças dos Ovinos/etiologia , Doenças dos Ovinos/metabolismo , Doenças dos Ovinos/fisiopatologia , Estresse Fisiológico/etiologia , Estresse Fisiológico/fisiopatologia , Estresse Fisiológico/prevenção & controle , Meios de TransporteRESUMO
Astrocytomas represent the most common cerebral tumors in humans and in animals, and the fibrillary cytological subtype is the most frequently observed. In this report and for the first time, a thalamic astrocytoma is described in a chamois showing depressed mentation, pleurothotonus and circling to the right side.
Assuntos
Astrocitoma/veterinária , Neoplasias Encefálicas/veterinária , Doenças das Cabras/diagnóstico , Rupicapra , Animais , Astrocitoma/diagnóstico , Astrocitoma/fisiopatologia , Neoplasias Encefálicas/diagnóstico , Neoplasias Encefálicas/fisiopatologia , Evolução Fatal , Doenças das Cabras/patologia , Doenças das Cabras/fisiopatologia , Cabras , Masculino , Exame Neurológico/veterináriaRESUMO
Thirty-six West African Dwarf (WAD) goats were used to assess the effects of an experimental Trypanosoma congolense infection on their reproductive system. Estrous cycles were synchronised and when confirmed pregnant (n = 31), the does were randomly allocated into control and trypanosome-infected groups. After infection, the animals were carefully observed till parturition. Trypanosome infection caused an increase of rectal temperature, a significant drop in PCV (infected: 23.3 +/- 0.3%; control: 28.5 +/- 0.4%; P < 0.0001) and abortions in 27.8% of the infected does. Kids born from infected does had a lower birth weight than kids born from control goats (0.9 +/- 0.1 kg versus 1.6 +/- 0.1 kg; P < 0.0001). Eight out of 13 kids (61.5%) that were born alive from infected does died during their first week of life. Plasma pregnancy-associated glycoprotein (PAG) and progesterone concentrations were lower in the infected animals than in the controls. In general, PAG concentration in does which aborted dropped before abortion. Our results revealed that artificial T. congolense infection affected reproductive performance of WAD goats with abortions, premature births and perinatal losses being observed. Neither transplacental transmission of T. congolense nor histopathological lesions of the placenta could be demonstrated.
Assuntos
Doenças das Cabras/parasitologia , Reprodução , Trypanosoma congolense , Tripanossomíase Africana/veterinária , Aborto Animal/parasitologia , Animais , Animais Recém-Nascidos , Peso ao Nascer , Sincronização do Estro , Feminino , Glicoproteínas/sangue , Doenças das Cabras/fisiopatologia , Cabras , Gravidez , Proteínas da Gravidez/sangue , Nascimento Prematuro/parasitologia , Nascimento Prematuro/veterinária , Progesterona/sangue , Tripanossomíase Africana/sangue , Tripanossomíase Africana/fisiopatologiaRESUMO
Enzootic intranasal tumor (EIT) appears glandular in type and has recently been classified as an adenocarcinoma of low malignancy. The aim of this study was to characterize the secretion of surface glycoconjugates (GCs) in EIT and in normal respiratory and olfactory mucosae of the goat by means of conventional and lectin histochemistry, in order to shed light on the histogenesis of EIT. Morphologic and ultrastructural investigations showed two growth types of EIT: i.e., tubular and papillary patterns. Conventional histochemistry revealed the presence of neutral and carboxylated GCs in the olfactory glands and in the tubular part of EIT, as well neutral and sulphated GCs in the respiratory glands and in the papillary part of EIT, suggesting that the papillary pattern tumor arises from the respiratory glands, whereas the tubular portion of EIT arises from the olfactory glands. Lectin histochemistry gave further information on the expressed GCs.
Assuntos
Adenocarcinoma/veterinária , Glicoconjugados/metabolismo , Doenças das Cabras/patologia , Neoplasias Nasais/veterinária , Mucosa Olfatória/patologia , Adenocarcinoma/metabolismo , Adenocarcinoma/patologia , Animais , Doenças das Cabras/fisiopatologia , Cabras , Histocitoquímica , Lectinas/química , Neoplasias Nasais/metabolismo , Neoplasias Nasais/patologia , Mucosa Olfatória/metabolismoRESUMO
Death, abortion, decreased milk yield and emaciation in dairy goats occurred due to calcinosis in a goat operation on 1100 meter sea level in Tyrol. This could be diagnosed by the clinical, sonographical and pathomorphological investigation. In the basic fodder yellow oat grass proportion was present with 40%. Clinically the apathic goats showed reduced appetite, emaciation, dyspnea, anaemic mucous membranes, heart noises and less activity to move. The blood-chemical examination of one goat revealed an increased activity of AP (199 U/l), calcium and phosphor-concentrations were in normal range. Sonographically liquidothorax and -perikard, calcified pulmonary and aortic valves and a thickened aortic orifice as well as calcification of kidneys, enlargment of liver and ascites could be diagnosed in this goat. The sonographical findings were confirmed by pathomorphological examination.
Assuntos
Aborto Animal/epidemiologia , Calcinose/veterinária , Doenças das Cabras/epidemiologia , Animais , Calcinose/epidemiologia , Calcinose/mortalidade , Calcinose/fisiopatologia , Surtos de Doenças/veterinária , Feminino , Alemanha/epidemiologia , Doenças das Cabras/mortalidade , Doenças das Cabras/fisiopatologia , Cabras , Leite , Gravidez , Útero/patologiaRESUMO
The in vitro reactivity of monocyte-derived macrophages (MDM) from Oestrus ovis (O. ovis) artificially infested lambs and kids was determined by measuring their production of nitric oxide (NO) during the course of infestation. In both species, crude antigenic preparations obtained from O. ovis first instar larvae (L1) were found to significantly (P < 0.01) inhibit this NO production, whereas O. ovis second instar (L2) extract stimulated it. Furthermore, this NO production by MDM decreased during infestation and was related to blood eosinophilia. It appears that crude antigenic extract from O. ovis modified the NO activity of macrophages from lambs and kids infested with O. ovis larvae.
Assuntos
Dípteros/crescimento & desenvolvimento , Ectoparasitoses/veterinária , Doenças das Cabras/fisiopatologia , Macrófagos/fisiologia , Monócitos/fisiologia , Doenças dos Ovinos/fisiopatologia , Animais , Células Cultivadas , Ectoparasitoses/fisiopatologia , Eosinófilos/fisiologia , Doenças das Cabras/parasitologia , Cabras , Larva , Contagem de Leucócitos , Estágios do Ciclo de Vida , Óxido Nítrico/biossíntese , Nitritos/metabolismo , Ovinos , Doenças dos Ovinos/parasitologiaRESUMO
The right mammary gland of 12 lactating goats was inoculated intracisternally with 1 ml of Mycoplasma capricolum subsp. capripneumoniae (Mcc) containing 10(6) colony-forming units (CFU), while their left mammary halves received 1 ml of sterile PPLO broth only. Two goats served as uninfected controls. The clinical mastitis that developed in the infected mammary halves within 24 h was initially acute but became increasingly chronic by the end of the experiment at 24 days post inoculation (DPI). The disease was characterized by atrophy of the infected mammary halves, leading to marked agalactia and an increase in somatic cell counts, with a preponderance of neutrophils initially and lymphocytes later. The Mycoplasma was re-isolated from infected mammary secretions up to 16 DPI but not from blood. Histopathology revealed that the mastitis was acute and purulent initially, followed by infiltration of lymphonuclear cells and fibroplasia in the lymphomononuclear cells and fibroplasia in the interacinar tissue, and later by massive fibrosis. Immunohistology demonstrated the presence of Mycoplasma-like bodies localized mainly on the surface of acinar/duct epithelial cells. The studies showed that Mcc was highly pathogenic in the caprine mammary gland.
Assuntos
Doenças das Cabras/microbiologia , Glândulas Mamárias Animais/microbiologia , Mastite/veterinária , Infecções por Mycoplasma/veterinária , Mycoplasma/patogenicidade , Animais , Atrofia/veterinária , Bovinos , Contagem de Colônia Microbiana/veterinária , Feminino , Técnica Indireta de Fluorescência para Anticorpo/veterinária , Doenças das Cabras/patologia , Doenças das Cabras/fisiopatologia , Cabras , Técnicas Imunoenzimáticas/veterinária , Lactação , Glândulas Mamárias Animais/patologia , Glândulas Mamárias Animais/fisiopatologia , Mastite/microbiologia , Mastite/fisiopatologia , Leite/microbiologia , Mycoplasma/fisiologia , Infecções por Mycoplasma/microbiologia , Infecções por Mycoplasma/fisiopatologia , CoelhosRESUMO
OBJECTIVE: To determine the pathogenic potential of an adenovirus isolated from a goat. ANIMALS: 14 colostrum-deprived, isolation-reared goat kids approximately 3 weeks old. PROCEDURE: Kids were inoculated with either cell culture fluid containing adenovirus (n = 10) or uninfected cell culture fluid (n = 4): 2 ml transtracheally and 1 ml/nostril. Clinical signs of disease and rectal temperature were recorded daily; nasal secretion and fecal specimens were collected daily. Control kids were necropsied, 2/d, on postinoculation days (PID) 5 and 10. Virus-inoculated kids were necropsied on PID 3, 5, 7, 10, and 28. After necropsy, lung, liver, kidney, and brain specimens were aseptically collected for virus isolation attempts. Tracheal fluid was collected on sterile cotton swabs. Turbinate, trachea, lung, mediastinal lymph node, liver, kidney, duodenum, jejunum, ileum, mesenteric lymph node, colon, and brain specimens were collected for histologic evaluation. RESULTS: Kids developed mild-to-moderate clinical respiratory tract infection. Virus was recovered consistently from nasal secretion and sporadically from fecal specimens. Grossly, there were multiple areas of atelectasis and hyperemia, principally in the cranioventral portion of the lungs. Microscopically, there was detachment and sloughing of foci of epithelial cells of the terminal bronchioles and alveoli. In kids necropsied late in the disease, these changes were accompanied by hyperplasia of type-II epithelial cells. Viral inclusions were not an obvious feature, but a few cells contained probable inclusions. CONCLUSIONS AND CLINICAL RELEVANCE: The caprine adenovirus reported here is capable of inducing respiratory tract disease and lesions in the lungs of young kids.
Assuntos
Infecções por Adenoviridae/veterinária , Adenoviridae/patogenicidade , Doenças das Cabras/virologia , Infecções Respiratórias/veterinária , Adenoviridae/imunologia , Adenoviridae/isolamento & purificação , Infecções por Adenoviridae/imunologia , Infecções por Adenoviridae/virologia , Animais , Anticorpos Antivirais/sangue , Temperatura Corporal/fisiologia , Brônquios/química , Brônquios/patologia , Brônquios/virologia , Colostro/fisiologia , Doenças das Cabras/imunologia , Doenças das Cabras/fisiopatologia , Cabras , Pulmão/química , Pulmão/patologia , Pulmão/virologia , Alvéolos Pulmonares/química , Alvéolos Pulmonares/patologia , Alvéolos Pulmonares/virologia , Infecções Respiratórias/imunologia , Infecções Respiratórias/virologiaAssuntos
Acalasia Esofágica/veterinária , Doenças das Cabras/fisiopatologia , Timoma/veterinária , Neoplasias da Glândula Tireoide/veterinária , Animais , Acalasia Esofágica/etiologia , Feminino , Cabras , Timoma/complicações , Timoma/fisiopatologia , Neoplasias da Glândula Tireoide/complicações , Neoplasias da Glândula Tireoide/fisiopatologiaAssuntos
Doenças das Cabras/fisiopatologia , Fase Luteal/fisiologia , Complicações na Gravidez/veterinária , Progesterona/sangue , Desnutrição Proteico-Calórica/veterinária , Animais , Feminino , Doenças das Cabras/sangue , Cabras , Fase Luteal/sangue , Masculino , Gravidez , Complicações na Gravidez/sangue , Complicações na Gravidez/fisiopatologia , Desnutrição Proteico-Calórica/sangue , Desnutrição Proteico-Calórica/fisiopatologia , Distribuição AleatóriaRESUMO
Unilateral intramammary inoculation of 10 goats with Cryptococcus neoformans (2 x 10(6) yeast cells) resulted in the development of mastitis, with gross and microscopic lesions being restricted to the infected udder halves only and there was no dissemination of infection to the opposite uninfected udder halves as well as to other organs of the body. The experiment was continued for 40 days, with 2 animals each from the infected and control groups being killed on 5th, 10th, 20th, 30th and 40th day post-inoculation (DPI). Initial enlargement of the infected udder halves was followed by marked decrease in size leading to very small, firm and nodular udder halves. After infection, there was also sharp fall in the milk yield. Cryptococcal organisms were demonstrated in the mastitic milk and udder impression smears with special stains. C. neoformans was reisolated from the milk of the only infected udder halves up to 25th DPI. Microscopically, there was initially acute diffuse purulent mastitis which later on became chronic, characterised by marked infiltration of lymphocytes, macrophages, extensive fibrosis and development of multiple granulomas. The cryptococcal organisms could be demonstrated in the udder sections only up to 30th DPI. It is concluded that intramammary inoculation of Cryptococcus neoformans in goats leads to severe mastitis with sharp fall in milk yield.
Assuntos
Criptococose/veterinária , Doenças das Cabras/patologia , Mastite/veterinária , Animais , Criptococose/patologia , Criptococose/fisiopatologia , Feminino , Fibrose , Doenças das Cabras/microbiologia , Doenças das Cabras/fisiopatologia , Cabras , Lactação , Linfócitos/patologia , Glândulas Mamárias Animais/metabolismo , Glândulas Mamárias Animais/patologia , Mastite/patologia , Mastite/fisiopatologia , Leite/citologia , Leite/metabolismo , Leite/microbiologia , Neutrófilos/patologia , Fatores de TempoRESUMO
Eight pregnant does were housed individually and fed a hay and concentrate diet throughout pregnancy and lactation. The mean gestation period was 146.7 +/- 3.0 days, with a twinning rate of 75 per cent. Mean body condition scores improved from 2.4 +/- 0.2 to 2.8 +/- 0.2 over the first 80 days of gestation and were maintained at 2.8 until 45 days before kidding. From then until kidding, mean scores fell to 2.2 +/- 0.2. Plasma progesterone concentrations during pregnancy rose significantly from 3.91 +/- 0.51 ng/ml on day 40 to 5.96 +/- 0.51 ng/ml on day 60 (P < 0.05) and remained high until 5 days before kidding. Three pseudopregnant does had similar progesterone profiles to pregnant does over the first 80 days, but the rise around day 35 to 40 was not significant and progesterone concentrations returned gradually to basal levels after day 100. The same 8 does, together with an additional 4 does which had been brought inside 60 to 70 days before kidding, were used to study onset of ovarian activity post partum. The twinning percentage was 83 per cent. Mean body condition score at parturition was 2.2 +/- 0.1. By day 35 post partum, mean condition scores had fallen to 1.9 +/- 0.1, and mean weights from 36.9 +/- 1.9 kg at kidding to 32.1 +/- 2.0 kg. Ovarian cyclicity was resumed just before mean scores and weights started to improve. The mean interval from kidding to onset of oestrous cycles was 97.3 +/- 9.5 days. This coincided with mean time to weaning which was 99.5 +/- 5.5 days.(ABSTRACT TRUNCATED AT 250 WORDS)