RESUMO
PURPOSE: To determine whether excimer laser ablation of guttae is a viable strategy for removal of diseased tissue in Fuchs' endothelial corneal dystrophy (FECD) on excised human Descemet membranes and whether an excimer laser-created wound on healthy human corneas ex vivo is recolonized with corneal endothelial cells. METHODS: Descemet membranes of FECD patients and corneal endothelium of normal human corneas were ablated ex vivo using an excimer laser licensed for glaucoma surgery. Specimens were kept in cell culture medium supplemented with 10 µm of rho-kinase inhibitor ripasudil. Corneal endothelial cell regeneration was observed using light and electron scanning microscopy. Furthermore, the whole corneal samples were evaluated by haematoxylin/eosin staining and immunohistochemical analysis using antibodies against Na+ /K+ -ATPase. RESULTS: Guttae and corneal endothelium could be ablated with an excimer laser without total ultrastructural damage to the Descemet membrane or stroma. Nearly complete endothelial wound closure was accomplished after 26-38 days in treated corneas. Light and electron scanning microscopy suggested the establishment of a layer of flat endothelial cells. Additionally, Na+ /K+ -ATPase expression could only be observed on the inner side of the Descemet membrane. CONCLUSION: Our proof of concept study demonstrated that excimer lasers can be used to ablate diseased tissue from excised FECD Descemet membranes ex vivo. Additionally, corneal endothelial cells recolonize a previously ablated endothelial area in healthy human corneas ex vivo under treatment with ripasudil. Thus, our results are the first experimental basis to further investigate the feasibility of an excimer laser ablation as a graftless FECD treatment option.
Assuntos
Lâmina Limitante Posterior/cirurgia , Endotélio Corneano/cirurgia , Distrofia Endotelial de Fuchs/cirurgia , Catarata , Córnea/cirurgia , Transplante de Córnea/métodos , Lâmina Limitante Posterior/ultraestrutura , Endotélio Corneano/ultraestrutura , Humanos , Terapia a Laser/métodos , Lasers de Excimer , Estudo de Prova de ConceitoRESUMO
PURPOSE: To compare the corneal tissue trauma after the use of an automated preloaded injector and a manual injector and assess scanning electron microscope (SEM) and atomic force microscope (AFM) features of both injector cartridges. SETTING: Ophthalmology Clinic and Laboratory of Stem Cells and Regenerative Medicine University "G. d'Annunzio" of Chieti-Pescara, Chieti, Italy; DESIGN: Prospective randomized clinical study METHODS: Forty eyes of 40 patients for phacoemulsification were divided into two groups: implantation of intraocular lens was performed with AutonoMe automated delivery system (AutonoMe group: 20 eyes) and Monarch III injector system (Monarch group: 20 eyes). In vivo confocal microscopy (IVCM) and anterior segment optical coherence tomography (AS-OCT) were performed before surgery, at 1 h, 1 day and 1 month post-operatively. In addition, SEM and AFM were performed on cartridges of both injector systems after injection of the IOL. RESULTS: A greater increase in central corneal thickness and corneal thickness at the incision site were observed in Monarch group versus AutonoMe group 1 h and 1 day post-operatively (p < 0.05). Endothelial cell count loss was significantly higher in Monarch group compared with AutonoMe group (p < 0.05) at 1 and 24 h. AS-OCT showed less endothelial misalignment at 30 days (p < 0.05), and IVCM showed less tunnel inflammation at all time points (p < 0.05) in AutonoMe group compared with Monarch group; roughness analysis at AFM of the AutonoMe cartridge was significantly lower compared to Monarch D cartridge (p < 0.05). CONCLUSIONS: The AutonoMe injector provided less corneal tissue trauma compared with Monarch III injector. The AutonoMe cartridge showed lower roughness at AFM compared to the Monarch D cartridge.
Assuntos
Catarata/diagnóstico por imagem , Implante de Lente Intraocular/instrumentação , Lentes Intraoculares , Facoemulsificação/métodos , Idoso , Endotélio Corneano/ultraestrutura , Desenho de Equipamento , Feminino , Seguimentos , Humanos , Masculino , Microscopia Eletrônica de Varredura , Estudos Prospectivos , Tomografia de Coerência ÓpticaRESUMO
PURPOSE: Loss of corneal endothelial cells (CECs) bears disastrous consequences for the patient, including corneal clouding and blindness. Corneal transplantation is currently the only therapy for severe corneal disorders. However, the worldwide shortages of corneal donor material generate a strong demand for personalized stem cell-based alternative therapies. Because human mesenchymal stem cells are known to be sensitive to their mechanical environments, we investigated the mechanotransductive potential of Descemet membrane-like microtopography (DLT) to differentiate human mesenchymal stem cells into CEC-like cells. METHODS: Master molds with inverted DLT were produced by 2-photon lithography (2-PL). To measure the mechanotransductive potential of DLT, mesenchymal stem cells were cultivated on silicone or collagen imprints with DLT. Changes in morphology were imaged, and changes in gene expression of CEC typical genes such as zonula occludens (ZO-1), sodium/potassium (Na/K)-ATPase, paired-like homeodomain 2 (PITX2), and collagen 8 (COL-8) were measured with real-time polymerase chain reaction. At least immunofluorescence analysis has been conducted to confirm gene data on the protein level. RESULTS: Adhesion of MSCs to DLT molded in silicone and particularly in collagen initiates polygonal morphology and monolayer formation and enhances not only transcription of CEC typical genes such as ZO-1, Na/K-ATPase, PITX2, and COL-8 but also expression of the corresponding proteins. CONCLUSIONS: Artificial reproduction of Descemet membrane with respect to topography and similar stiffness offers a potential innovative way to bioengineer a functional CEC monolayer from autologous stem cells.
Assuntos
Doenças da Córnea/cirurgia , Transplante de Córnea , Lâmina Limitante Posterior/ultraestrutura , Endotélio Corneano/ultraestrutura , Células-Tronco Mesenquimais/ultraestrutura , Fotomicrografia/métodos , Animais , Biomimética , Contagem de Células , Células Cultivadas , Doenças da Córnea/patologia , Citometria de Fluxo , Humanos , Masculino , Microscopia Eletrônica de Varredura , CoelhosAssuntos
Doenças da Córnea/diagnóstico por imagem , Microscopia Confocal/métodos , Mieloma Múltiplo/complicações , Idoso de 80 Anos ou mais , Doenças da Córnea/etiologia , Doenças da Córnea/patologia , Substância Própria/ultraestrutura , Cristalização , Endotélio Corneano/ultraestrutura , Epitélio Corneano/ultraestrutura , Feminino , Humanos , Cadeias Leves de Imunoglobulina/análise , Mieloma Múltiplo/patologia , Proteínas do Mieloma/análise , Microscopia com Lâmpada de FendaRESUMO
Purpose: Corneal endothelial cell density undergoes a progressive decrease for many years after transplantation, eventually threatening patients with late endothelial failure. The purpose of this study was to investigate the possibility of an immunologic response in successfully grafted corneal endothelium. Methods: The corneal endothelium of patients who had undergone corneal transplantation was evaluated by specular microscopy. Rabbit models were subjected to penetrating keratoplasty (PK) with either syngeneic or allogeneic corneal transplants and Descemet's stripping endothelial keratoplasty (DSEK) with allogeneic corneal transplants. The presence of immune cells and expression of proinflammatory cytokines were determined by immunostaining. The corneal endothelium and immune cells were also evaluated by scanning electron microscopy. Results: Scanning slit contact specular microscopy of patients with no features of graft rejection revealed cell-like white dots on the grafted corneal endothelium. The corneal endothelium of the allogeneic PK and DSEK rabbit models displayed the presence of immune cells, including CD4+ T-helper cells, CD8+ cytotoxic T cells, CD20+ B lymphocytes, CD68+ macrophages, and neutrophils, but these immune cells were rarely observed in the syngeneic PK model. These immune cells also produced proinflammatory cytokines. Notably, some of the corneal endothelial cells situated near these immune cells exhibited features of apoptosis. Conclusions: T lymphocytes, B lymphocytes, macrophages, and neutrophils are present on the grafted corneal endothelium in both PK and DSEK allogeneic rabbit models. The potential involvement of immune cells as an underlying pathophysiology for late endothelial failure deserves further examination.
Assuntos
Ceratoplastia Endotelial com Remoção da Lâmina Limitante Posterior/métodos , Endotélio Corneano/imunologia , Rejeição de Enxerto/imunologia , Imunidade Celular , Linfócitos T/imunologia , Adulto , Idoso , Animais , Contagem de Células , Doenças da Córnea/cirurgia , Citocinas/metabolismo , Modelos Animais de Doenças , Progressão da Doença , Endotélio Corneano/ultraestrutura , Feminino , Rejeição de Enxerto/metabolismo , Rejeição de Enxerto/patologia , Sobrevivência de Enxerto , Humanos , Macrófagos/imunologia , Macrófagos/ultraestrutura , Masculino , Microscopia Eletrônica de Varredura , Pessoa de Meia-Idade , Neutrófilos/imunologia , Neutrófilos/ultraestrutura , Coelhos , Transplante HomólogoRESUMO
PURPOSE: Some forms of keratoplasty assisted by ultrashort-pulse lasers require performing laser cuts close to the endothelium, which requires the knowledge of "safe" values concerning incision depth and pulse energy preserving endothelial cell viability. Our study aims to determine the thresholds for cell death in porcine corneas exposed to ultrashort laser pulses, in terms of laser pulse energy and nearness of the impacts to the endothelium. METHODS: Using a laboratory laser set-up, lamellar cuts were induced while varying pulse energies and distances from the endothelium. A fluorescent staining protocol was used to determine the percentage of surviving endothelial cells. Numerical simulations of the Euler equations for compressible fluids provided pressure level and axial and radial pressure gradient estimates at the endothelium. RESULTS: Ninety percent of the endothelial cells survived when using 16.5 µJ pulses no closer than 200 µm to the endothelium, or pulses not exceeding 2 µJ at a distance of 50 µm. The comparison of the observed percentage of surviving cells with the estimates of the shock wave amplitudes and gradients generated by the laser pulses yielded cell death thresholds at amplitudes in the megapascal range, or gradients of the order of 108 Pa/m. CONCLUSIONS: Our results provide limits in terms of pulse energy and distance of the incision from the endothelium within which endothelial cell viability is preserved. Current forms of corneal laser surgery are compatible with these limits. However, these limits will need to be considered for the development of future laser routines working in close proximity to the endothelium.
Assuntos
Cirurgia da Córnea a Laser/métodos , Endotélio Corneano/ultraestrutura , Animais , Morte Celular , Sobrevivência Celular , Endotélio Corneano/cirurgia , Microscopia Eletrônica de Varredura , Modelos Animais , Suínos , Tomografia de Coerência ÓpticaRESUMO
Purpose: Engineered corneal endothelial grafts able to provide numerous functional endothelial cells for the restoration of corneal transparency would be a worthwhile way of replacing donor tissue, which is extremely scarce. The grafts are simply constructed: a biocompatible thin and transparent carrier colonized by a monolayer of cultured endothelial cells (ECs). Here we describe a process able to obtain appropriate carriers by recycling human corneas unsuitable for graft in their original state, but liable to provide multiple thin lamellae when cut with a femtosecond laser as used in refractive surgery. Methods: We selected a robust method of stromal decellularization. To demonstrate that neither this process nor long-term storage hindered cell adherence, lamellae were endothelialized with an EC line. Results: The constructs achieved up to very high EC density (the main quality criterion for regular donor corneas) while remaining transparent and thin. We verified that they could be inserted in the anterior chamber of a human eye, like a conventional endothelial graft. Human decellularized cornea will likely be directly compatible with the recipient cornea and comply with the requirements of health regulatory authorities. Conclusions: This study demonstrates that thin human corneal lamellae could have high potential as carriers in next-generation therapy for endothelial dysfunctions.
Assuntos
Bioengenharia/métodos , Substância Própria/transplante , Transplante de Córnea/métodos , Endotélio Corneano/transplante , Coleta de Tecidos e Órgãos/métodos , Obtenção de Tecidos e Órgãos/métodos , Idoso , Idoso de 80 Anos ou mais , Contagem de Células , Células Cultivadas , Distrofias Hereditárias da Córnea/cirurgia , Substância Própria/ultraestrutura , Endotélio Corneano/ultraestrutura , Humanos , Lasers , Microscopia Eletrônica de Varredura , Pessoa de Meia-Idade , Fatores de Tempo , Doadores de TecidosRESUMO
PURPOSE: To assess the suitability of a new 345 nm ultraviolet (UV) femtosecond laser for refractive surgery. SETTING: Department of Ophthalmology, University of Erlangen-Nürnberg, Erlangen, Germany. DESIGN: Experimental study. METHODS: Twenty-five porcine corneas were used for stromal flap or lamellar bed creation (stromal depth, 150 µm) and 15 rabbit corneas for lamellar bed creation near the endothelium. Ultraviolet femtosecond laser cutting-line morphology, gas formation, and keratocyte death rate were evaluated using light and electron microscopy and compared with a standard infrared (IR) femtosecond laser. Endothelial cell survival was examined after application of a laser cut near the endothelium. RESULTS: Flaps created by the UV laser were lifted easily. Gas formation was reduced 4.2-fold compared with the IR laser (P = .001). The keratocyte death rate near the interface was almost doubled; however, the death zone was confined to a region within 38 µm ± 10 (SD) along the cutting line. Histologically and ultrastructurally, a distinct and continuous cutting line was not found after UV femtosecond laser application if flap lifting was omitted and standard energy parameters were used. Instead, a regular pattern of vertical striations, presumably representing self-focusing induced regions of optical tissue breakdown, were identified. Lamellar bed creation with standard energy parameters 50 µm from the endothelium rendered the endothelial cells intact and viable. CONCLUSION: The new 345 nm femtosecond laser is a candidate for pending in vivo trials and future high-precision flap creation, intrastromal lenticule extraction, and ultrathin Descemet-stripping endothelial keratoplasty. FINANCIAL DISCLOSURES: Mr. Klenke and Ms. Skerl were paid employees of Wavelight GmbH when the study was performed. Dr. Seiler is a scientific consultant to Wavelight GmbH. No other author has a financial or proprietary interest in any material or method mentioned.
Assuntos
Substância Própria/cirurgia , Lasers de Excimer/uso terapêutico , Retalhos Cirúrgicos , Raios Ultravioleta , Animais , Sobrevivência Celular , Ceratócitos da Córnea/ultraestrutura , Substância Própria/patologia , Endotélio Corneano/ultraestrutura , Marcação In Situ das Extremidades Cortadas , Microbolhas , Microscopia Eletrônica , Coelhos , SuínosRESUMO
PURPOSE: To investigate the outcome of Descemet membrane endothelial keratoplasty (DMEK) in patients with graft failure after Descemet stripping automated endothelial keratoplasty (DSAEK). DESIGN: Retrospective cohort study. METHODS: setting: Institutional. STUDY POPULATION: Fifteen eyes of 15 patients that underwent DMEK for graft failure with corneal decompensation following DSAEK were analyzed; 15 eyes with primary DMEK for Fuchs corneal dystrophy were included as control group. MAIN OUTCOME MEASURES: Best-corrected visual acuity (BCVA), endothelial cell density (ECD), central corneal thickness (CCT), and rebubbling rate. RESULTS: DMEK surgery was successful in all cases of both groups. Mean BCVA (logMAR) before DMEK was 1.27 ± 0.34 in the DMEK after DSAEK group and 1.0 ± 0.40 in the Primary DMEK group. After DMEK, mean BCVA increased significantly to 0.23 ± 0.21 (P = .012, DMEK after DSAEK group) and 0.29 ± 0.23 (P = .042, Primary DMEK group) after 3 months. There were no significant differences in mean BCVA between both groups at each visit. The rebubbling rate was 13% in the DMEK after DSAEK group and 40% in the Primary DMEK group (P = .1). Mean CCT decreased significantly in both groups 1 month after DMEK (P < .05). Mean ECD and change of ECD did not differ significantly between both groups at each visit (P > .05). CONCLUSION: The results after DMEK as a procedure to treat graft failure after DSAEK were as good as in patients that underwent DMEK as primary intervention to treat advanced Fuchs dystrophy. This indicates that the optical quality can be reestablished by DMEK in patients with failed DSAEK.
Assuntos
Ceratoplastia Endotelial com Remoção da Lâmina Limitante Posterior/métodos , Distrofia Endotelial de Fuchs/cirurgia , Rejeição de Enxerto/cirurgia , Complicações Pós-Operatórias , Idoso , Contagem de Células , Estudos de Coortes , Paquimetria Corneana , Endotélio Corneano/patologia , Endotélio Corneano/ultraestrutura , Feminino , Rejeição de Enxerto/etiologia , Humanos , Masculino , Microscopia Eletrônica de Transmissão , Estudos Retrospectivos , Acuidade Visual/fisiologiaRESUMO
PURPOSE: To evaluate the effects of intracameral injection of ranibizumab and bevacizumab on the corneal endothelium by scanning electron microscopy (SEM). METHODS: Twenty-eight female rabbits were randomly divided into four equal groups. Rabbits in groups 1 and 2 underwent intracameral injection of 1 mg/0.1 mL and 0.5 mg/0.05 mL ranibizumab, respectively; group 3 was injected with 1.25 mg/0.05 mL bevacizumab. All three groups were injected with a balanced salt solution (BSS) into the anterior chamber of the left (fellow) eye. None of the rabbits in group 4 underwent an injection. Corneal thickness and intraocular pressure were measured before the injections, on the first day, and in the first month after injection. The rabbits were sacrificed and corneal tissues were excised in the first month after injection. Specular microscopy was used for the corneal endothelial cell count. Endothelial cell density was assessed and comparisons drawn between the groups and the control. Micrographs were recorded for SEM examination. The structure of the corneal endothelial cells, the junctional area of the cell membrane, the distribution of microvillus, and the cell morphology of the eyes that underwent intracameral injection of vascular endothelial growth factor (VEGF), BSS, and the control group were compared. RESULTS: Corneal thickness and intraocular pressure were not significantly different between the groups that underwent anti-VEGF or BSS injection and the control group on the first day and in the first month of injection. The corneal endothelial cell count was significantly diminished in all three groups; predominantly in group 1 and 2 (P<0.05). The SEM examination revealed normal corneal endothelial histology in group 3 and the control group. Eyes in group 1 exhibited indistinctness of corneal endothelial cell borders, microvillus loss in the luminal surface, excessive blebbing, and disintegration of intercellular junctions. In group 2, the cell structure of the corneal endothelium and intercellular junctions were normal. However, a relative reduction was observed in the microvillus density of endothelial cells. Although eyes in group 3 were morphologically similar to fellow eyes and the control group, disarrangement in endothelial cell borders was evident. CONCLUSION: The SEM examination pointed out deterioration in endothelial cell morphology after intracameral injection of 1 and 0.5 mg ranizumab. However, the effects of intracameral bevacizumab injection on corneal endothelial cells were similar to those found in fellow eyes and the control group. Further large-scale studies that examine the cellular changes by transmission electron microscopy are required to support the results of the present study that evaluates the structural changes in endothelial cells by SEM.
Assuntos
Anticorpos Monoclonais Humanizados/farmacologia , Endotélio Corneano/efeitos dos fármacos , Animais , Bevacizumab , Córnea/efeitos dos fármacos , Córnea/ultraestrutura , Endotélio Corneano/ultraestrutura , Feminino , Injeções Intraoculares , Pressão Intraocular/efeitos dos fármacos , Microscopia Eletrônica de Varredura/métodos , Coelhos , Distribuição Aleatória , Ranibizumab , Fator A de Crescimento do Endotélio Vascular/farmacologiaRESUMO
PURPOSE: To evaluate the role of preexisting corneal pathology on the outcome of Descemet membrane endothelial keratoplasty (DMEK), and also to evaluate the long-term outcome of repeat DMEK for graft failure after primary DMEK. METHODS: Eighteen patients undergoing repeat DMEK after failed DMEK were enrolled; 9 of 18 patients had successful primary DMEK on the fellow eye. Evaluations included preoperative anterior chamber depth, intraoperative degree of difficulty, transmission electron microscopy images (n = 14), best-corrected visual acuity (BCVA), endothelial cell density, central corneal thickness, corneal volume, and patient satisfaction. RESULTS: Surgeries that led to graft failure had a higher intraoperative degree of difficulty compared with successful surgeries (P = 0.002). Eight of 14 failed grafts showed ultrastructural abnormalities, that is, inclusions or deposits of abnormal fibrillar material in Descemet membrane, indicating endothelial dysfunction before transplantation. BCVA on day 10 after surgery was worse in eyes with graft failure compared with successful DMEK (P = 0.008). Median BCVA (logarithm of the minimum angle of resolution) improved from 0.5 before DMEK and 1.9 before repeat DMEK to 0.3 at 1-year follow-up (P = 0.011). One year after repeat DMEK, endothelial cell density (cells/mm2) of donor corneas decreased from 2501 ± 264 to 1373 ± 270 (P < 0.001), central corneal thickness (µm) decreased from 807 ± 160 to 576 ± 178 (P = 0.002), and corneal volume (mm3) decreased from 84.1 ± 13.0 to 64.4 ± 12.5 (P = 0.002). Patient satisfaction showed no difference between primary and repeat DMEK. CONCLUSIONS: A preexisting subclinical corneal endothelial dysfunction may contribute to primary DMEK failure. Repeat DMEK can be performed safely with good long-term outcome.
Assuntos
Perda de Células Endoteliais da Córnea/patologia , Lâmina Limitante Posterior/ultraestrutura , Ceratoplastia Endotelial com Remoção da Lâmina Limitante Posterior , Endotélio Corneano/ultraestrutura , Rejeição de Enxerto/patologia , Idoso , Contagem de Células , Paquimetria Corneana , Feminino , Distrofia Endotelial de Fuchs/cirurgia , Rejeição de Enxerto/etiologia , Rejeição de Enxerto/cirurgia , Humanos , Masculino , Microscopia Eletrônica de Transmissão , Satisfação do Paciente , Reoperação , Doadores de Tecidos , Acuidade Visual/fisiologiaRESUMO
PURPOSE: Corneal tissue shortage has become a major concern worldwide, which has motivated the search for alternative solutions to eye bank human eyes for corneal transplantation. Minimally invasive lamellar transplantation and tissue engineering may offer new opportunities for the rehabilitation of diseased corneas. The aim of this study was to evaluate the biocompatibility and functionality of stromal lamellar grafts tissue-engineered (TE) in vitro and transplanted in vivo in the cornea of a feline model. METHODS: The corneal stromas were engineered in culture from corneal stromal cells using the self-assembly approach, without the addition of exogenous material or scaffold. Eight healthy animals underwent two intrastromal grafts in one eye and the contralateral eye was used as a control. Animals were followed with slit-lamp ophthalmic examination, corneal esthesiometry and optical coherent tomography. Confocal microscopy, immunofluorescence, histology, and transmission electron microscopy (TEM) were performed at 4 months. RESULTS: Four months after transplantation, the TE-stromal grafts were transparent, functional, and well tolerated by the eye. All grafts remained avascular, with no signs of immune rejection, despite a short course of low-dose topical steroids. Corneal sensitivity returned to preoperative level and reinnervation of the grafts was confirmed by confocal microscopy and immunofluorescence. Histology and TEM of the TE-grafts showed a lamellar stromal structure with regular collagen fibril arrangement. CONCLUSIONS: These results open the way to an entirely new therapeutic modality. Intracorneal filling using a biocompatible, transparent, and malleable TE-stroma could be the basis for multiple types of novel therapeutic options in corneal interventional surgery.
Assuntos
Substância Própria/transplante , Transplante de Córnea/métodos , Engenharia Tecidual , Adulto , Animais , Gatos , Células Cultivadas , Substância Própria/ultraestrutura , Modelos Animais de Doenças , Endotélio Corneano/ultraestrutura , Sobrevivência de Enxerto , Teste de Histocompatibilidade/métodos , Humanos , Microscopia Confocal , Tomografia de Coerência Óptica , Transplante HeterólogoRESUMO
PURPOSE: To describe a new technique of endothelial keratoplasty (EK) that improves the quality of lamellar dissection of donor cornea. METHODS: We compared four techniques of donor cornea preparation for lamellar dissection on 8 donor corneas: mechanical dissection with a microkeratome, a single femtosecond laser lamellar cut, a double femtosecond laser lamellar cut and combined femtosecond laser lamellar dissection with excimer laser surface photoablation. The quality of the donor cornea interface was assessed and compared using scanning electron microscopy (SEM), and the most satisfactory technique was employed for EK on three patients. The postoperative anatomic results were analyzed with anterior segment spectral-domain optical coherence tomography (SD-OCT) and in vivo confocal microscopy (IVCM). RESULTS: The smoothest stromal interface was observed on SEM with the combined use of femtosecond laser dissection and excimer photoablation. The surgical procedures performed with donor cornea prepared by a combination of femtosecond and excimer lasers resulted in clear corneas after 1 month. SD-OCT showed good attachment of the endothelial graft and a hyperreflective interface. On IVCM, subepithelial haze, honeycomb-like activated keratocytes and needle-shaped particles were visible in the recipient corneal stroma as well as numerous hyperreflective particles on the donor-recipient interface. CONCLUSION: A new technique, femtosecond and excimer laser-assisted endothelial keratoplasty (FELEK), which refines the current limitations observed in Descemet-stripping automated endothelial keratoplasty (DSAEK), is described. Femtosecond laser dissection provides a thin and reproducible endothelial graft cut with a high level of safety and accuracy, while excimer photoablation yields a smooth, high-quality interface.
Assuntos
Transplante de Córnea/métodos , Endotélio Corneano/transplante , Terapia a Laser , Lasers de Excimer , Idoso , Idoso de 80 Anos ou mais , Endotélio Corneano/ultraestrutura , Feminino , Humanos , Técnicas In Vitro , Microscopia Eletrônica de Varredura , Pessoa de Meia-Idade , Fatores de TempoRESUMO
AIM: To evaluate the stromal bed quality and endothelial damage after femtosecond laser (FSL) cuts into the deep corneal stroma. METHODS: Using a 150-kHz FSL, a lamellar cut was aimed at a depth of 100, 300, or 500 µm in porcine corneas. Stromal bed smoothness was graded from light microscopy and scanning electron microscopy images. Rabbit corneas were cut at remaining thicknesses of 70, 100 and 150 µm using the FSL. The effects of peeling off the corneal flap and the distance between laser spots (2 or 4 µm) were examined. RESULTS: The ratio of damaged cells in the group with a remaining depth of 70 µm was significantly larger than that in the groups with a remaining depth of 150 µm. The ratio of damaged cells in the group with a 4-µm spot separation and the flap peeled off was significantly larger than that in the group with a 4-µm spot separation and the flap not peeled off. CONCLUSIONS: Corneal endothelial damage is likely to increase when the remaining depth is less than 70 µm, and peeling off the flap damages corneal endothelial cells when the remaining depth is less than 100 µm.
Assuntos
Substância Própria/patologia , Transplante de Córnea , Endotélio Corneano/lesões , Terapia a Laser , Animais , Paquimetria Corneana , Substância Própria/diagnóstico por imagem , Substância Própria/cirurgia , Modelos Animais de Doenças , Endotélio Corneano/ultraestrutura , Microscopia Eletrônica de Varredura , Suínos , UltrassonografiaRESUMO
The corneal endothelium (CE) is a single layer of cells lining the posterior face of the cornea providing metabolic functions essential for maintenance of corneal transparency. Adult CE cells lack regenerative potential, and the number of CE cells decreases throughout life. To determine whether endogenous DNA damage contributes to the age-related spontaneous loss of CE, we characterized CE in Ercc1(-/Δ) mice, which have impaired capacity to repair DNA damage and age prematurely. Eyes from 4.5- to 6-month-old Ercc1(-/Δ) mice, age-matched wild-type (WT) littermates, and old WT mice (24- to 34-month-old) were compared by spectral domain optical coherence tomography and corneal confocal microscopy. Histopathological changes in CE were further identified in paraffin tissue sections, whole-mount immunostaining, and scanning electron and transmission electron microscopy. The CE of old WT mice displayed polymorphism and polymegathism, polyploidy, decreased cell density, increased cell size, increases in Descemet's thickness, and the presence of posterior projections originating from the CE toward the anterior chamber, similar to changes documented for aging human corneas. Similar changes were observed in young adult Ercc1(-/Δ) mice CE, demonstrating spontaneous premature aging of the CE of these DNA repair-deficient mice. CD45(+) immune cells were associated with the posterior surface of CE from Ercc1(-/Δ) mice and the tissue expressed increased IL-1α, Cxcl2, and TNFα, pro-inflammatory proteins associated with senescence-associated secretory phenotype. These data provide strong experimental evidence that DNA damage can promote aging of the CE and that Ercc1(-/Δ) mice offer a rapid and accurate model to study CE pathogenesis and therapy.
Assuntos
Envelhecimento/patologia , Doenças da Córnea/metabolismo , Doenças da Córnea/patologia , Reparo do DNA , Endotélio Corneano/metabolismo , Endotélio Corneano/patologia , Animais , Apoptose , Contagem de Células , Colágeno/metabolismo , Proteínas de Ligação a DNA/deficiência , Proteínas de Ligação a DNA/metabolismo , Lâmina Limitante Posterior/metabolismo , Lâmina Limitante Posterior/patologia , Lâmina Limitante Posterior/ultraestrutura , Endonucleases/deficiência , Endonucleases/metabolismo , Células Endoteliais/metabolismo , Células Endoteliais/patologia , Células Endoteliais/ultraestrutura , Endotélio Corneano/crescimento & desenvolvimento , Endotélio Corneano/ultraestrutura , Humanos , Antígenos Comuns de Leucócito/metabolismo , Camundongos , FenótipoRESUMO
PURPOSE: To assess the reproducibility of manual graft preparation and evaluate the incidence rate and nature of structural anomalies of Descemet's membrane (DM) preventing successful graft preparation in DM endothelial keratoplasty (DMEK). DESIGN: Prospective, single-center, nonrandomized, consecutive case series. PARTICIPANTS: We analyzed 350 corneoscleral buttons from donors aged 18-95 years stored in Optisol-GS or Dulbecco's modified Eagle's medium and used for DMEK surgery in 343 consecutive patients with Fuchs' endothelial dystrophy or pseudophakic bullous keratopathy. METHODS: Residual endothelial cell-DM complexes obtained after successful DM stripping for DMEK and whole donor corneas obtained after unsuccessful DM stripping were examined by transmission electron microscopy and immunohistochemistry. MAIN OUTCOME MEASURES: Accuracy of the cleavage plane between DM and corneal stroma and structural abnormalities of the DM-stroma interface. RESULTS: Uneventful manual separation without any disruption of DM was achieved in 335 of 350 donor corneas (95.7%) by use of a previously established bimanual submerged preparation technique. Correspondingly, the peeled DM specimens revealed a regular and smooth cleavage plane exposing the amorphous interfacial matrix on their anterior surface. Although 8 of 350 donor corneas (2.3%) showed focal adhesions of DM to the corneal stroma and developed isolated tears during stripping, preparation of the graft could be successfully completed. However, 7 of the 350 donor corneas (2.0%) showed extremely strong adhesion and multiple tears of DM, preventing successful preparation of the graft. These specimens revealed either ultrastructural (peg-like interlockings) or biochemical abnormalities (increased staining intensities for adhesive glycoproteins) along the DM-stroma interface. CONCLUSIONS: Using an appropriate technique, manual preparation of grafts for DMEK with reproducible tissue qualities is possible in the vast majority (98%) of donor corneas. Although a relatively rare phenomenon, interindividual variations in DM structure and composition may be responsible for failure of graft preparation in about 2% of donor corneas. FINANCIAL DISCLOSURE(S): The authors have no proprietary or commercial interest in any of the materials discussed in this article.
Assuntos
Lâmina Limitante Posterior/ultraestrutura , Ceratoplastia Endotelial com Remoção da Lâmina Limitante Posterior/métodos , Endotélio Corneano/ultraestrutura , Coleta de Tecidos e Órgãos/normas , Adolescente , Adulto , Idoso , Idoso de 80 Anos ou mais , Biomarcadores/metabolismo , Contagem de Células , Lâmina Limitante Posterior/metabolismo , Endotélio Corneano/metabolismo , Endotélio Corneano/transplante , Proteínas da Matriz Extracelular , Bancos de Olhos , Fibronectinas/metabolismo , Técnica Indireta de Fluorescência para Anticorpo , Sobrevivência de Enxerto/fisiologia , Humanos , Pessoa de Meia-Idade , Técnicas de Cultura de Órgãos , Osteonectina/metabolismo , Estudos Prospectivos , Refração Ocular/fisiologia , Reprodutibilidade dos Testes , Manejo de Espécimes/métodos , Manejo de Espécimes/normas , Doadores de Tecidos , Coleta de Tecidos e Órgãos/métodos , Fator de Crescimento Transformador beta , Acuidade Visual/fisiologia , Vitronectina/metabolismo , Adulto JovemRESUMO
BACKGROUND: The aim of this study was to compare the visual outcome of femtosecond laser-assisted Descemet stripping automated endothelial keratoplasty (DSAEK) to microkeratome-assisted DSAEK as well as to contrast precut versus surgeon-cut grafts. Histologic characterization of failed DSAEK grafts was performed in order to correlate ultrastructural changes with graft failures. METHODS: In this case control study, 47 cases of DSAEK were investigated in terms of visual acuity, keratometric astigmatism, spherical equivalent, endothelial cell count, and postoperative complications. We formed three groups: the femtosecondlaser-assisted DSAEK with precut grafts, the microkeratome-assisted DSAEK with precut and with surgeon-cut grafts. Mean follow-up was 6 months. In the case of graft failure, penetrating keratoplasty was performed, and the excised corneal buttons were investigated by light and electron microscopy. RESULTS: Microkeratome-assisted DSAEK lead to better visual outcome than femtosecond laser-assisted DSAEK. Keratometric astigmatism, spherical equivalent and endothelial cell count did not differ significantly between both methods. Precut and surgeon-cut grafts in microkeratome-assisted DSAEK did not show any significant difference regarding all upraised parameters. No definite histological correlate for graft failure following femtosecond laser-assisted DSAEK was found. CONCLUSIONS: Femtosecond laser-assisted DSAEK is not the method of choice, and needs further technical improvement. However, failed femtosecondlaser-assisted DSAEK grafts did not show significant histological changes related to the technique to explain reduced visual acuity. In microkeratome-assisted DSAEK, the preparation time point of the graft does not seem to influence the visual and optical outcome.
Assuntos
Distrofias Hereditárias da Córnea/cirurgia , Perda de Células Endoteliais da Córnea/patologia , Ceratoplastia Endotelial com Remoção da Lâmina Limitante Posterior/métodos , Endotélio Corneano/ultraestrutura , Lasers de Excimer/uso terapêutico , Acuidade Visual/fisiologia , Idoso , Astigmatismo/etiologia , Biomarcadores/metabolismo , Estudos de Casos e Controles , Contagem de Células , Distrofias Hereditárias da Córnea/fisiopatologia , Endotélio Corneano/cirurgia , Feminino , Rejeição de Enxerto/etiologia , Rejeição de Enxerto/metabolismo , Rejeição de Enxerto/cirurgia , Sobrevivência de Enxerto , Humanos , Ceratoplastia Penetrante , Masculino , Técnicas de Cultura de Órgãos , Reoperação , Doadores de TecidosRESUMO
PURPOSE: To evaluate the effect of Rho kinase (ROCK)-inhibitor eye drops on a corneal endothelial dysfunction primate model and human clinical case series of corneal endothelial dysfunction. METHODS: As a corneal-endothelial partially injured model, the corneal endothelium of seven cynomolgus monkeys was damaged by transcorneal freezing; 10 mm of rock inhibitor Y-27632 was then applied topically 6 times daily. The phenotype of the reconstructed corneal endothelium was evaluated by immunohistochemical analysis and noncontact specular microscopy. For clinical study, the effect of Y-27632 eye drops after transcorneal freezing was evaluated in eight corneal endothelial dysfunction patients: four central corneal edema patients and four diffuse corneal edema patients. RESULTS: Slit-lamp microscopy revealed that both Y-27632-treated and -nontreated corneas became hazy after transcorneal freezing, and then recovered their transparency within 4 weeks. ROCK inhibitor Y-27632 promoted recovery of corneal endothelial cell density and wound healing in terms of both morphology and function. The percentage of ZO-1 and Na(+)/K(+)-ATPase positive cells in the regenerated area in the Y-27632 group was significantly higher than in the controls. Noncontact specular microscopy revealed that corneal endothelial cell density was significantly higher in the Y-27632 group compared with the controls at 4 weeks; cell density reached approximately 3000 cells/mm(2), as opposed to 1500 cells/mm(2) in the control group. In addition to the animal study findings, the clinical study findings showed that Y-27632 eye drops effectively improved corneal edema of corneal endothelial dysfunction patients with central edema. CONCLUSIONS: These findings show that rock inhibitor Y-27632 eye drops promote corneal endothelial wound healing in a primate animal model and suggest the possibility of Y-27632 as a novel therapeutic modality for certain forms of corneal endothelial dysfunction. (http://www.umin.ac.jp/ctr/ number, UMIN000003625.).
Assuntos
Amidas/uso terapêutico , Edema da Córnea/tratamento farmacológico , Modelos Animais de Doenças , Inibidores Enzimáticos/uso terapêutico , Piridinas/uso terapêutico , Cicatrização/efeitos dos fármacos , Ferimentos não Penetrantes/tratamento farmacológico , Quinases Associadas a rho/antagonistas & inibidores , Administração Tópica , Idoso , Animais , Contagem de Células , Edema da Córnea/metabolismo , Edema da Córnea/patologia , Perda de Células Endoteliais da Córnea/fisiopatologia , Endotélio Corneano/enzimologia , Endotélio Corneano/lesões , Endotélio Corneano/ultraestrutura , Traumatismos Oculares/tratamento farmacológico , Traumatismos Oculares/metabolismo , Traumatismos Oculares/patologia , Feminino , Humanos , Macaca fascicularis , Masculino , Pessoa de Meia-Idade , Soluções Oftálmicas , ATPase Trocadora de Sódio-Potássio/metabolismo , Ferimentos não Penetrantes/metabolismo , Ferimentos não Penetrantes/patologia , Proteína da Zônula de Oclusão-1/metabolismoRESUMO
PURPOSE: To determine the short-term fate of the host endothelium and Descemet membrane after non-Descemet stripping automated endothelial keratoplasty (nDSAEK). METHODS: Eight unilateral DSAEK (n = 4) or nDSAEK (n = 4) surgeries were performed in the right eyes of 8 rabbits. Corneal transparency and thickness were followed-up by slit-lamp microscopy, and 2 weeks postoperatively, corneas were evaluated by immunohistochemistry and transmission electron microscopy. RESULTS: Corneas remained clear after both DSAEK and nDSAEK. One week after DSAEK, the stroma-to-stroma surgical interface was identifiable as a zone of fibrotic tissue a few microns thick, whereas in the nDSAEK group, the recipient corneal endothelium and Descemet membrane were clearly visible at the graft-host interface. The retained endothelial cells were positive for Na/K-ATPase but assumed a markedly different morphology from healthy endothelial cells, with cell processes extending into the graft stroma or engulfing strands of irregularly dissected grafted stromal tissue where they occasionally appeared to compartmentalize the transplanted matrix and became detached from the underlying Descemet membrane. CONCLUSIONS: Host endothelial cells 2 weeks after nDSAEK express markers of pump function, but appear to be morphologically altered, occasionally detaching from the adjacent Descemet membrane, extending into the graft stroma or engulfing strands of the grafted stroma at the interface. The short-term persistence and subsequent phenotypical alternation of residual endothelial cells, aligned to structural changes to Descemet membrane, might influence graft adherence after nDSAEK.
Assuntos
Lâmina Limitante Posterior/ultraestrutura , Ceratoplastia Endotelial com Remoção da Lâmina Limitante Posterior , Endotélio Corneano/ultraestrutura , Animais , Córnea/fisiologia , Lâmina Limitante Posterior/enzimologia , Endotélio Corneano/enzimologia , Feminino , Técnica Indireta de Fluorescência para Anticorpo , Microscopia Eletrônica de Transmissão , Microscopia de Fluorescência , Coelhos , ATPase Trocadora de Sódio-Potássio/metabolismoRESUMO
Contact inhibition ubiquitously exists in non-transformed cells that are in contact with neighboring cells. This phenomenon explains the poor regenerative capacity of in vivo human corneal endothelial cells during aging, injury and surgery. This study demonstrated that the conventional approach of expanding human corneal endothelial cells by disrupting contact inhibition with EDTA followed by bFGF activated canonical Wnt signaling and lost the normal phenotype to endothelial-mesenchymal transition, especially if TGFß1 was added. By contrast, siRNA against p120 catenin (CTNND1) also uniquely promoted proliferation of the endothelial cells by activating trafficking of p120 catenin to the nucleus, thus relieving repression by nuclear Kaiso. This nuclear p120-catenin-Kaiso signaling is associated with activation of RhoA-ROCK signaling, destabilization of microtubules and inhibition of Hippo signaling, but not with activation of Wnt-ß-catenin signaling. Consequently, proliferating human corneal endothelial cells maintained a hexagonal shape, with junctional expression of N-cadherin, ZO-1 and Na(+)/K(+)-ATPase. Further expansion of human corneal endothelial monolayers with a normal phenotype and a higher density was possible by prolonging treatment with p120 catenin siRNA followed by its withdrawal. This new strategy of perturbing contact inhibition by selective activation of p120-catenin-Kaiso signaling without disrupting adherent junction could be used to engineer surgical grafts containing normal human corneal endothelial cells to meet a global corneal shortage and for endothelial keratoplasties.