RESUMO
Our previous studies with shishijimicin A resulted in the total synthesis of this scarce marine natural product and a number of its simpler analogues endowed with picomolar potencies against certain cancer cell lines. Herein, we describe the design, synthesis, and biological evaluation of four linker-drugs, anticipating the construction of antibody-drug conjugates (ADCs) as the ultimate goal of this research program. Using a common payload, the assembly of these linker-drugs utilized different linkers and attachment points, providing opportunities to probe the optimal molecular design of the intended ADCs as targeted cancer therapies. In the course of ADC generation and in vitro evaluation, we identified two linker-drugs with a promising in vitro plasma stability profile and excellent targeted cytotoxicity and specificity. Conjugation of shishijimicin A enediyne payloads through their phenolic moiety represents a novel approach to enediyne ADC creation, while the pharmacological profiles of at least two of the generated ADCs compare well with the profiles of the corresponding clinically approved ADC Kadcyla.
Assuntos
Antineoplásicos/farmacologia , Carbolinas/farmacologia , Dissacarídeos/farmacologia , Enedi-Inos/farmacologia , Imunoconjugados/farmacologia , Antineoplásicos/síntese química , Antineoplásicos/química , Carbolinas/síntese química , Carbolinas/química , Sobrevivência Celular/efeitos dos fármacos , Dissacarídeos/síntese química , Dissacarídeos/química , Desenho de Fármacos , Enedi-Inos/síntese química , Enedi-Inos/química , Células HEK293 , Humanos , Imunoconjugados/química , Estrutura MolecularRESUMO
The family of anthraquinone-fused enediyne antitumor antibiotics was established by the discovery of dynemicin A and deoxy-dynemicin A. It was then expanded, first by the isolation of uncialamycin, and then by the addition to the family of tiancimycins A-F and yangpumicin A. This family of natural products provides opportunities in total synthesis, biology, and medicine due to their novel and challenging molecular structures, intriguing biological properties and mechanism of action, and potential in targeted cancer therapies. Herein, the total syntheses of tiancimycins A and B, yangpumicin A, and a number of related anthraquinone-fused enediynes are described. Biological evaluation of the synthesized compounds revealed extremely potent cytotoxicities against a number of cell lines, thus enriching the structure-activity relationships within this class of compounds. The findings of these studies may facilitate future investigations directed toward antibody-drug conjugates for targeted cancer therapies and provide inspiration for further advances in total synthesis and chemical biology.
Assuntos
Antraquinonas/química , Antibióticos Antineoplásicos/farmacologia , Enedi-Inos/síntese química , Enedi-Inos/farmacologia , Antibióticos Antineoplásicos/química , Humanos , Relação Estrutura-AtividadeRESUMO
The kedarcidin chromophore is a formidible target for total synthesis. Herein, we describe a viable synthesis of this highly unstable natural product. This entailed the early introduction and gram-scale synthesis of 2-deoxysugar conjugates of both L-mycarose and L-kedarosamine. Key advances include: (1) stereoselective allenylzinc keto-addition to form an epoxyalkyne; (2) α-selective glycosylations with 2-deoxy thioglycosides (AgPF6/DTBMP) and Schmidt donors (TiCl4); (3) Mitsunobu aryl etherification to install a hindered 1,2-cis-configuration; (4) atropselective and convergent Sonogashira-Shiina cyclization sequence; (5) Ohfune-based amidation protocol for naphthoic acid; (6) Ce(III)-mediated nine-membered enediyne cyclization and ester/mesylate derivatisation; (7) SmI2-based reductive olefination and global HF-deprotection end-game. The longest linear sequence from gram-scale intermediates is 17-steps, and HRMS data of the synthetic natural product was obtained for the first time.
Assuntos
Cicloparafinas/síntese química , Enedi-Inos/síntese química , Naftalenos/síntese química , Antineoplásicos/síntese química , Antineoplásicos/química , Cicloparafinas/química , Enedi-Inos/química , Estrutura Molecular , Naftalenos/químicaRESUMO
Shishijimicin A is a scarce marine natural product with highly potent cytotoxicities, making it a potential payload or a lead compound for designed antibody-drug conjugates. Herein, we describe an improved total synthesis of shishijimicin A and the design, synthesis, and biological evaluation of a series of analogues. Equipped with appropriate functionalities for linker attachment, a number of these analogues exhibited extremely potent cytotoxicities for the intended purposes. The synthetic strategies and tactics developed and employed in these studies included improved preparation of previously known and new sulfenylating reagents such as PhthNSSMe and related compounds.
Assuntos
Antibióticos Antineoplásicos/síntese química , Carbolinas/síntese química , Dissacarídeos/síntese química , Enedi-Inos/síntese química , Indicadores e Reagentes/síntese química , Antibióticos Antineoplásicos/farmacologia , Carbolinas/farmacologia , Linhagem Celular Tumoral , Ciclização , Reação de Cicloadição , Dissacarídeos/farmacologia , Desenho de Fármacos , Enedi-Inos/farmacologia , Glicosilação , Células HEK293 , Humanos , Estereoisomerismo , Relação Estrutura-AtividadeRESUMO
BACKGROUND: The first reports of the natural enediyne anticancer antibiotics date back to the late 1980s; since then, a great deal of interest has been devoted to the chemistry, biology and potential medical applications of this family of compounds. The biological activity of enediynes is linked to the presence of a highly unsaturated hex-1-ene-1,5-diyne system. The thermally induced transformation of this unit into a benzene σ-σ diradical (the Bergman cycloaromatization) is the key step of the antitumor properties of such compounds: 1,4-diaryl radicals are able to abstract H-atoms from the deoxyribose backbone of DNA, thus leading to DNA strand cleavage and ultimately cell death. METHODS: We undertook a structured search of bibliographic databases for peer-reviewed research literature using focused and high quality papers. Research efforts addressed at understanding and mimicking the various processes involved in the targeting, activation and DNA cleavage associated with these products are described. The potential of a great number of non natural enediynes in the treatment of many infectious diseases, apart their role in anticancer drugs, such as antibacterial activity, protein degradation activity, has been reported Results: Due to the interesting mode of action of this class of compounds, the unique molecular architecture of enediynes has been exploited towards the synthesis of many non natural compounds in order to study and enhance their biological properties. Seventy-six papers were included in this review. It is divided in paragraphs that include: Carbo- oxygen-nitrogen- and sulfur- enediynes, polymers and macrocycles. The synthetic approaches to the different classes of compounds are discussed in detail together with the biological implications of the synthesized compounds Conclusion: The review summarizes the most recent advances in the synthesis and reactivity of non natural enediynes by focusing the attention particularly to the biological properties of the most interesting members of the family of carbo- and hetero- enediynes. The findings of this review confirm the importance of non natural enediynes as potential drugs in the treatment of cancer and many infectious diseases.
Assuntos
Antineoplásicos/síntese química , Antineoplásicos/uso terapêutico , Técnicas de Química Sintética/métodos , Enedi-Inos/síntese química , Enedi-Inos/uso terapêutico , Neoplasias/tratamento farmacológico , Animais , Antineoplásicos/química , Antineoplásicos/farmacologia , Clivagem do DNA/efeitos dos fármacos , Enedi-Inos/química , Enedi-Inos/farmacologia , Humanos , Neoplasias/genéticaRESUMO
The compounds produced by a living organism are most commonly as medicinal agents and starting materials for the preparation of new semi-synthetic derivatives. One of the largest groups of natural compounds consists of products containing a 1,4-benzoquinone subunit. This fragment occurs in three enediyne antibiotics, dynemicin A, deoxydynemicin A, and uncilamicin, which exhibit high biological activity. A series of alkoxy derivatives containing 1,4-naphthoquinone, 5,8-quinolinedione, and 2-methyl-5,8-quinolinedione moieties was synthesized. Moreover, the 1,4-benzoquinone subunit was contacted with an enediyne fragment. All obtained compounds were characterized by spectroscopy and spectrometry methods. The resulting alkane, alkene, alkyne and enediyne derivatives were tested as antitumor agents. They showed high cytotoxic activity depending on the type of 1,4-benzoquinone subunit and the employed tumor cell lines. The synthesized derivatives fulfill the Lipinski Rule of Five and have low permeability through the blood-brain barrier.
Assuntos
Antineoplásicos Fitogênicos/síntese química , Benzoquinonas/química , Enedi-Inos/síntese química , Quinolinas/síntese química , Antraquinonas/síntese química , Antraquinonas/farmacologia , Antineoplásicos Fitogênicos/farmacologia , Produtos Biológicos/química , Produtos Biológicos/farmacologia , Linhagem Celular Tumoral , Sobrevivência Celular/efeitos dos fármacos , Enedi-Inos/farmacologia , Humanos , Concentração Inibidora 50 , Naftoquinonas/química , Especificidade de Órgãos , Quinolinas/farmacologia , Relação Estrutura-AtividadeRESUMO
The total synthesis of the rare but extremely potent antitumor agent shishijimicin A has been achieved via a convergent strategy involving carboline disaccharide 3 and hydroxy enediyne thioacetate 4.
Assuntos
Antineoplásicos/síntese química , Produtos Biológicos/síntese química , Carbolinas/síntese química , Dissacarídeos/síntese química , Enedi-Inos/síntese química , Compostos de Sulfidrila/química , Antineoplásicos/química , Produtos Biológicos/química , Carbolinas/química , Dissacarídeos/química , Enedi-Inos/química , Compostos de Sulfidrila/síntese químicaRESUMO
We developed a synthetic scheme for the synthesis of naturally occurring (14R)-oenanthotoxin and several analogs. Key-steps of this synthesis were an efficient homo-coupling of alkynes and a chemoenzymatic resolution of racemic oenanthotoxin using novozyme 435 and vinyl acetate. The compounds were screened for their cytotoxic activity using a photometric sulforhodamine B assays and several human tumor cell lines. Oenanthotoxin and many derivatives thereof were cytotoxic to tumor cell lines as well as to non-malignant mouse fibroblasts. The highest activity was determined for human ovarian cancer cells A2780 with EC50 = 3.8 µM.
Assuntos
Enedi-Inos/química , Enedi-Inos/síntese química , Álcoois Graxos/química , Álcoois Graxos/síntese química , Antineoplásicos/farmacologia , Humanos , Estrutura MolecularRESUMO
The enediynes are known for highly potent anticancer, antimicrobial, as well as cytotoxic activities. The discovery of enediynes from natural sources was achieved in late 1980s. They are presently of high interest, because they exert their biological action due to their ability to form a diradical, which abstracts H-atoms from the DNA backbone, thus causing cell death. Nowadays, the major works are dedicated to the syntheses of enediynes. This review covers recent developments in enediyne chemistry of the last few decades. It is subdivided in six chapters dealing with the discussion of the chemistry and biological significances of enediynes, and the factors responsible for a better activation of enediynes and potent biological evaluations.
Assuntos
Enedi-Inos/química , Anti-Infecciosos/química , Anti-Infecciosos/farmacologia , Antineoplásicos/química , Antineoplásicos/farmacologia , Antineoplásicos/uso terapêutico , Produtos Biológicos/química , Dano ao DNA/efeitos dos fármacos , Ensaios de Seleção de Medicamentos Antitumorais , Enedi-Inos/síntese química , Humanos , Compostos Macrocíclicos/química , Metais/química , Neoplasias/tratamento farmacológico , Porfirinas/químicaRESUMO
We herein describe the synthesis of 15 novel 13-membered cyclic enediyne derivatives using simple and straightforward approach. Representative examples were screened for their anticancer activities on 60 different human tumor cell lines representing various histologies viz. leukemia, melanoma, and cancers of lung, colon, kidney, ovary, breast, prostate, and central nervous system. The enediyne derivatives with halogen substitutions, especially fluorides were found to be active against most of the cell lines. The initial results indicates marginal to good inhibition for the growth of tumor cells for several cell lines, which shows the potential of these class of compound towards anticancer application.
Assuntos
Antineoplásicos/síntese química , Antineoplásicos/farmacologia , Enedi-Inos/síntese química , Enedi-Inos/farmacologia , Neoplasias/tratamento farmacológico , Antineoplásicos/química , Linhagem Celular Tumoral , Ensaios de Seleção de Medicamentos Antitumorais , Enedi-Inos/química , Feminino , Humanos , Masculino , Células Tumorais CultivadasRESUMO
The purported structures of the peyssonenynes A and B isolated from Peyssonnelia caulifera, and considered to be geometric isomers at the acetoxyenediyne moiety, have been synthesized. The E and Z geometries of the synthetic compounds were secured by the magnitude of the (3)J(H9-C7) values measured using the EXSIDE band-variant of the gradient HSQC pulse sequence and by the chemical shifts of C(6). Comparison of the NMR data of the synthetic and natural products revealed that only those of the Z isomers matched, which correspond to peyssonenyne A. Using HPLC analysis it was found that peyssonenyne B must correspond to the sn-2 positional isomer of the Z sn-1/3 counterpart. The four synthetic sn-1/3 diastereomers are roughly equipotent as DNMT1 inhibitors when evaluated on a radioactive methyl transfer enzymatic assay after immunoprecipitation from K562 human leukemia cells with anti-DNMT1 antibody.
Assuntos
DNA (Citosina-5-)-Metiltransferases/antagonistas & inibidores , Enedi-Inos/síntese química , Inibidores Enzimáticos/síntese química , Ácidos Graxos Insaturados/síntese química , DNA (Citosina-5-)-Metiltransferase 1 , Enedi-Inos/antagonistas & inibidores , Enedi-Inos/farmacologia , Ácidos Graxos Insaturados/antagonistas & inibidores , Ácidos Graxos Insaturados/farmacologia , Humanos , Células K562 , Estrutura Molecular , Relação Estrutura-AtividadeRESUMO
OBJECTIVE: Lidamycin (LDM) can be dissociated to an apoprotein (LDP) and an active enediyne chromophore (AE). The detached AE can reassemble with its LDP-containing fusion protein to endow the latter with potent antitumor activity. However, the reassembly of AE with LDP is affected by several factors. Our aim was to optimize the assembly efficiency of the AE with a LDP-containing fusion protein and investigate the influence of several factors on the assembly efficacy. METHODS: A method based on RP-HPLC was developed to analyze the assembly rate, and an orthogonal experimental design L(9) (3(4)) was used to investigate the effects of temperature, assembly time, pH and molecular ratio of LDP-containing fusion protein to AE on the assembly rate. Furthermore, the determined optimum conditions for the assembly rate of the LDP-containing fusion protein with AE were applied and evaluated. RESULTS: A calibration curve based on the LDM micromolar concentration against the peak-area of AE by HPLC was obtained. The order in which individual factors in the orthogonal experiment affected the assembly rate were temperature>time>pH>molar ratio of AE to protein and all were statistically significant (P<0.01). The optimal assembly conditions were temperature at 10°C, time of 12 h, pH 7.0, and the molar ratio of AE: protein of 5:1. The assembly rate of AE with a LDP-containing fusion protein was improved by 23% after condition optimization. CONCLUSION: The assembly rate of chromophore of lidamycin with its LDP-containing fusion protein was improved after condition optimization by orthogonal design, and the optimal conditions described herein should prove useful for the development of this type of LDP-containing fusion protein.
Assuntos
Aminoglicosídeos/síntese química , Antibióticos Antineoplásicos/síntese química , Apoproteínas/química , Enedi-Inos/síntese química , Proteínas Recombinantes de Fusão/química , Anticorpos de Cadeia Única/química , Aminoglicosídeos/administração & dosagem , Aminoglicosídeos/química , Aminoglicosídeos/farmacologia , Antibióticos Antineoplásicos/administração & dosagem , Antibióticos Antineoplásicos/química , Antibióticos Antineoplásicos/farmacologia , Linhagem Celular Tumoral , Sobrevivência Celular , Cromatografia Líquida de Alta Pressão , Desenho de Fármacos , Enedi-Inos/administração & dosagem , Enedi-Inos/química , Enedi-Inos/farmacologia , HumanosRESUMO
We synthesized diyne triols based on gymnasterkoreayne and evaluated their cancer chemopreventive activities in terms of the chemopreventive index (CI) to reveal the structure-activity relationship, and discovered more active compounds than natural diynes.
Assuntos
Acetileno/análogos & derivados , Anticarcinógenos/química , Enedi-Inos/química , Acetileno/síntese química , Acetileno/química , Acetileno/uso terapêutico , Anticarcinógenos/síntese química , Anticarcinógenos/uso terapêutico , Enedi-Inos/síntese química , Enedi-Inos/uso terapêutico , Humanos , Relação Estrutura-AtividadeRESUMO
A variety of fragmentations and rearrangements can follow Bergman cyclization in enediynes equipped with acetal rings mimicking the carbohydrate moiety of natural enediyne antibiotics of the esperamicine and calchiamicine families. In the first step of all these processes, intramolecular H-atom abstraction efficiently intercepts the p-benzyne product of the Bergman cyclization through a six-membered TS and transforms the p-benzyne into a new more stable radical. Depending on the substitution pattern and reaction conditions, this radical follows four alternative paths: (a) abstraction of an external hydrogen atom, (b) O-neophyl rearrangement which transposes O- and C-atoms of the substituent, (c) fragmentation of the O-C bond in the acetal ring, or (d) fragmentation with elimination of the appended acetal moiety as a whole. Experiments with varying concentrations of external H-atom donor (1,4-cyclohexadiene) were performed to gain further insight into the competition between intermolecular H-abstraction and the fragmentations. The Thorpe-Ingold effect in gem-dimethyl substituted enediynes enhances the efficiency of fragmentation to the extent where it cannot be prevented even by a large excess of external H-atom donor. These processes provide insight into a possible mechanism of unusual fragmentation of esperamicin A(1) upon its Bergman cycloaromatization and lay foundation for a new approach for the conformational control of reactivity of these natural antitumor antibiotics. Such an approach, in conjunction with supramolecular constraints, may provide a plausible mechanism for resistance to enediyne antibiotics by the enediyne-producing microorganisms.
Assuntos
Antibacterianos/química , Antibacterianos/síntese química , Enedi-Inos/química , Enedi-Inos/síntese química , Simulação por Computador , Ciclização , Modelos Químicos , Conformação MolecularRESUMO
The proposed structure of the maduropeptin chromophore, the biologically active component of the highly potent chromoprotein antitumor antibiotics, was stereoselectively synthesized but did not satisfy the spectra of the natural product. We demonstrated that the correct structure is diastereomeric, which possesses an antipodal sugar moiety.
Assuntos
Enedi-Inos/química , Enedi-Inos/síntese químicaRESUMO
Methodology is outlined for the chemical synthesis of versatile photo-Bergman enediyne building blocks and their conjugates. Routes to both mono and bis conjugated enediyne templates are detailed together with representative examples of their bioconjugates, nanoconjugates, PEG derivatives and water soluble salts. The immunocompetence of antibody conjugates is retained, and application in the form of reagents for photodynamic therapy (PDT) advanced.
Assuntos
Antineoplásicos/síntese química , Enedi-Inos/síntese química , Fármacos Fotossensibilizantes/síntese química , Anticorpos/química , Anticorpos/imunologia , Antineoplásicos/química , Antineoplásicos/farmacologia , Enedi-Inos/química , Enedi-Inos/farmacologia , Ouro/química , Nanopartículas Metálicas/química , Fármacos Fotossensibilizantes/química , Fármacos Fotossensibilizantes/farmacologia , Polietilenoglicóis/químicaRESUMO
Novel enediyne-amino acid conjugates 1-4 have been synthesized. All of these effectively target the enzyme chymotrypsin inhibiting its proteolytic activity. The conjugate with a directly linked phenyl alanine is the most effective inhibitor with a K(i) of 3 microM. The mode of inhibition is mostly competitive or of a mixed type depending on the nature of the inhibitor.
Assuntos
Quimotripsina/antagonistas & inibidores , Desenho de Fármacos , Enedi-Inos/síntese química , Enedi-Inos/farmacologia , Inibidores Enzimáticos/farmacologia , Peptídeos/síntese química , Peptídeos/farmacologia , Ciclização , Enedi-Inos/química , Ativação Enzimática/efeitos dos fármacos , Estrutura Molecular , Peptídeos/química , Especificidade por SubstratoRESUMO
A series of novel pyrrolo[2,1-c][1,4]benzodiazepine (PBD) hybrids linked with enediyne is described. These compounds were prepared by linking C-8 of DC-81 (1) with an enediyne (10-16) through carbon chain linkers to afford PBD hybrid agents 17-23 in good yields. Most of the hybrids on human cancer cell lines exhibited higher cytotoxicity, and an increase in the sub-G1 population than 1. In a previous article, we have demonstrated that DC-81-indole conjugate agents (3-6) are potent inducers of cell apoptosis in melanoma. In the present article, we investigated whether DC-81-enediyne agents possess more cytotoxicity than 6 on human 293T cells. Our data revealed that treatment of 293T cells with DC-81-enediyne resulted in a significant increase of annexin V binding, caspase-3 degradation, and p53 arrest to identify apoptotic cells than 6. These results suggest that the DC-81-enediyne agents are more efficient in inducing apoptosis than DC-81-indole in 293T cells.
Assuntos
Antineoplásicos/síntese química , Antineoplásicos/farmacologia , Benzodiazepinas/síntese química , Benzodiazepinas/farmacologia , Enedi-Inos/síntese química , Antineoplásicos/química , Apoptose , Benzodiazepinas/química , Caspase 3/metabolismo , Linhagem Celular Tumoral , Ensaios de Seleção de Medicamentos Antitumorais , Enedi-Inos/química , Humanos , Indóis/síntese química , Indóis/química , Compostos Organofosforados/síntese química , Compostos Organofosforados/química , Compostos Organofosforados/farmacologia , Proteína Supressora de Tumor p53/metabolismoAssuntos
Antibióticos Antineoplásicos/síntese química , Cicloparafinas/síntese química , Enedi-Inos/síntese química , Naftalenos/síntese química , Antibióticos Antineoplásicos/química , Ciclização , Cicloparafinas/química , Enedi-Inos/química , Espectroscopia de Ressonância Magnética , Naftalenos/químicaRESUMO
A series of acyclic symmetry bis-enediynes have been synthesized successfully and their bioactivities were evaluated. Among them, 1,6-bis(4-((2-(pyridin-2-ylethynyl)phenyl)ethynyl)phenoxy)hexane 8g showed good inhibition activity against the CCRF-CEM (GI(50)=0.04 microM) and HL-60 (GI(50)=0.09 microM) cell lines of human leukemia. The cell cycle analysis shows that compound 8g arrests cell cycle via inhibiting Cyclin A and Cyclin B expressions in low concentration and induces a significant apoptosis progress in high concentration.