RESUMO
Cadmium (Cd) is a heavy metal related to a decrease in sperm parameters. The transit of spermatozoa through the epididymis is necessary to generate changes in the sperm membrane, such as the assembly of various carbohydrates that are added to the spermatazoan's surface to prepare it for successful fertilisation of the oocyte. No studies have yet analysed whether Cd alters the presence and distribution of these carbohydrates. We aimed to evaluate the changes induced by Cd in the distribution pattern of N-acetylglucosamine, sialic acid, mannose and fucose on the sperm membrane in the epididymis (e.g. caput, corpus, cauda) and if it alters the epididymal epithelium. Male Wistar pups were treated with Cd doses (0.125, 0.25 and 0.5mg/kg) on postnatal days 1-49. At postnatal day 90, they were humanely killed, sperm samples were obtained from the epididymis and tissue samples were taken for histological analysis. Cd concentrations in the blood and epididymis increased in proportion to the dose administered and decreased the serum testosterone levels and sperm quality. Histological analysis revealed alterations in the epithelium in all Cd-treated groups. Cd altered the distribution patterns of carbohydrates and fluorescence indices. All these alterations affected the structure and functioning of sperm.
Assuntos
Cádmio/administração & dosagem , Carboidratos/análise , Membrana Celular/química , Epididimo/crescimento & desenvolvimento , Maturação do Esperma/efeitos dos fármacos , Espermatozoides/crescimento & desenvolvimento , Acetilglucosamina/análise , Animais , Cádmio/análise , Membrana Celular/efeitos dos fármacos , Relação Dose-Resposta a Droga , Epididimo/química , Epididimo/citologia , Fucose/análise , Masculino , Manose/análise , Ácido N-Acetilneuramínico , Ratos , Ratos Wistar , Espermatozoides/efeitos dos fármacos , Espermatozoides/ultraestrutura , Testosterona/sangueRESUMO
Accumulating evidence shows that amyloids perform biological roles. We previously showed that an amyloid matrix composed of four members of the CRES subgroup of reproductive family 2 cystatins is a normal component of the mouse epididymal lumen. The cellular mechanisms that control the assembly of these and other functional amyloid structures, however, remain unclear. We speculated that cross-seeding between CRES members could be a mechanism to control the assembly of the endogenous functional amyloid. Herein we used thioflavin T assays and negative stain transmission electron microscopy to explore this possibility. We show that CRES3 rapidly formed large networks of beaded chains that possessed the characteristic cross-ß reflections of amyloid when examined by X-ray diffraction. The beaded amyloids accelerated the amyloidogenesis of CRES, a less amyloidogenic family member, in seeding assays during which beads transitioned into films and fibrils. Similarly, CRES seeds expedited CRES3 amyloidogenesis, although less efficiently than the CRES3 seeding of CRES. These studies suggest that CRES and CRES3 hetero-oligomerize and that CRES3 beaded amyloids may function as stable preassembled seeds. The CRES3 beaded amyloids also facilitated assembly of the unrelated amyloidogenic precursor Aß by providing a surface for polymerization though, intriguingly, CRES3 (and CRES) monomer/early oligomer profoundly inhibited Aß assembly. The cross-seeding between the CRES subgroup members is similar to that which occurs between bacterial curli proteins suggesting that it may be an evolutionarily conserved mechanism to control the assembly of some functional amyloids. Further, interactions between unrelated amyloidogenic precursors may also be a means to regulate functional amyloid assembly.
Assuntos
Amiloide/genética , Proteínas Amiloidogênicas/genética , Cistatinas/genética , Amiloide/química , Proteínas Amiloidogênicas/química , Animais , Benzotiazóis/química , Benzotiazóis/farmacologia , Cistatinas/química , Epididimo/química , Epididimo/crescimento & desenvolvimento , Masculino , Camundongos , Microscopia Eletrônica de Transmissão , Difração de Raios XRESUMO
Male infertility or subfertility is frequently associated with disruption of the hypothalamic-pituitary-testis axis events, like secondary hypogonadism. However, little is known how this condition affects the proteomic composition of the epididymal fluid. In the present study, we evaluated the proteomic changes in the cauda epididymal fluid (CEF) in a swine model of secondary hypogonadism induced by anti-GnRH immunization using multidimensional protein identification technology. Seven hundred and eighteen proteins were identified in both GnRH-immunized and control groups. GnRH immunization doubled the number of proteins in the CEF, with 417 proteins being found exclusively in samples from GnRH-immunized boars. CEF from GnRH-immunized boars presented an increase in the number of proteins related to cellular and metabolic processes, with affinity to organic cyclic compounds, small molecules, and heterocyclic compounds, as well changed the enzymatic profile of the CEF. Also, a significant increase in the number of proteins associated to the ubiquitin-proteasome system was identified in CEF from GnRH-immunized animals. These results bring strong evidence of the impact of secondary hypogonadism on the epididymal environment, which is responsible for sperm maturation and storage prior ejaculation. Finally, the differently expressed proteins in the CEF are putative seminal biomarkers for testicular and epididymal disorders caused by secondary hypogonadism.
Assuntos
Líquidos Corporais/metabolismo , Epididimo/metabolismo , Hipogonadismo/metabolismo , Infertilidade Masculina/metabolismo , Proteoma/metabolismo , Animais , Anticorpos/farmacologia , Líquidos Corporais/química , Líquidos Corporais/efeitos dos fármacos , Anticoncepção Imunológica/métodos , Anticoncepção Imunológica/veterinária , Epididimo/química , Epididimo/efeitos dos fármacos , Hormônio Liberador de Gonadotropina/imunologia , Hormônio Liberador de Gonadotropina/metabolismo , Hipogonadismo/etiologia , Hipogonadismo/imunologia , Sistema Hipotálamo-Hipofisário/efeitos dos fármacos , Sistema Hipotálamo-Hipofisário/metabolismo , Infertilidade Masculina/etiologia , Infertilidade Masculina/imunologia , Infertilidade Masculina/veterinária , Masculino , Modelos Animais , Proteoma/análise , Proteoma/efeitos dos fármacos , Proteômica , Transdução de Sinais/efeitos dos fármacos , Suínos/metabolismo , Testículo/efeitos dos fármacos , Testículo/metabolismoRESUMO
The protein disulphide isomerase A1 (PDIA1) is an important chaperone involved in protein quality control and redox regulation. Also, the ability of PDIA1 to bind to oestrogens suggests that it may play a role in epididymal maturation and male fertility. The goals of this study were to (a) verify the possible interaction between 17ß-estradiol and equine PDIA1 using bioinformatics; (b) identify and quantify PDIA1 protein in equine cauda epididymis throughout peripuberty; and (c) determine whether the amounts of PDIA1 in equine seminal plasma and spermatozoa are associated with fertility. Using in silico analysis, we were able to predict the tertiary structure of equine PDIA1 and to demonstrate the interaction between 17ß-estradiol and the putative binding site in domains b and b'. Colts under 24 months of age had lower relative amounts of PDIA1 in cauda epididymal fluid in comparison with older males (p < .01). No difference was observed in seminal plasma PDIA1 between fertile and subfertile stallions. Our study demonstrates that PDIA1 expression in the epididymis increases during peripuberty. However, in the adult stallion, its quantity in seminal plasma is not associated with fertility.
Assuntos
Epididimo/metabolismo , Cavalos/fisiologia , Isomerases de Dissulfetos de Proteínas/metabolismo , Sêmen/metabolismo , Maturidade Sexual/fisiologia , Animais , Biologia Computacional , Epididimo/química , Estradiol/química , Estradiol/metabolismo , Fertilidade , Masculino , Simulação de Acoplamento Molecular , Isomerases de Dissulfetos de Proteínas/análise , Isomerases de Dissulfetos de Proteínas/ultraestrutura , Estrutura Terciária de Proteína , Sêmen/químicaRESUMO
The sperm produced in the seminiferous tubules pass through the rete testis, efferent ducts, and epididymis. The epididymis has three distinct regions known as caput, corpus, and cauda. The transit through the epididymis is an essential process in sperm maturation. The lumen of each epididymal region has a unique fluid composition regulated by many ion channels and transporters in the epithelial cells. The objective of this study was to map the sites of localization of ion channels ENaC and CFTR along the length of the mouse and rat epididymis using confocal microscopic imaging. The integrity of the fine structure of the tissues was verified by fluorescent phalloidin staining of actin filaments visualized by high-resolution confocal microscopy. The 2D and 3D images showed preservation of the stereocilia. Based on these images we determined morphometric parameters of the epithelial cells and ducts. ENaC and CFTR immunofluorescence appeared almost continuously on the apical membrane of caput and in smooth muscle myoid cells. In cauda, CFTR expression was observed continuously in long stretches of epithelium interrupted by clusters of cells that showed no CFTR expression. Similar patterns of localization were observed in both mouse and rat samples. Mutations in the CFTR gene are known to result in male infertility. Based on the widespread presence of ENaC along the epididymis we suggest that mutations in ENaC subunits may also be associated with male infertility. The diverse phenotypes associated with CFTR mutations may be due to malfunction of CFTR at specific subcellular locations in the male reproductive system.
Assuntos
Regulador de Condutância Transmembrana em Fibrose Cística/análise , Epididimo/química , Canais Epiteliais de Sódio/análise , Animais , Regulador de Condutância Transmembrana em Fibrose Cística/genética , Células Epiteliais/química , Canais Epiteliais de Sódio/genética , Imunofluorescência , Infertilidade Masculina/genética , Espaço Intracelular/química , Masculino , Camundongos , Microscopia Confocal , Mutação , Ratos , Distribuição TecidualAssuntos
Epididimo/patologia , Neoplasias dos Genitais Masculinos/patologia , Tumor Neuroectodérmico Melanótico/patologia , Biomarcadores Tumorais/análise , Biópsia por Agulha , Epididimo/química , Epididimo/cirurgia , Neoplasias dos Genitais Masculinos/química , Neoplasias dos Genitais Masculinos/cirurgia , Humanos , Imuno-Histoquímica , Lactente , Masculino , Tumor Neuroectodérmico Melanótico/química , Tumor Neuroectodérmico Melanótico/cirurgia , Valor Preditivo dos TestesRESUMO
Motility of spermatozoa is a crucial factor for determining semen quality. Here we report motility inhibitory factor (MIF-II) from goat epididymal plasma, revealing its structure, function, localization and motility inhibitory pathway. Structural characterization with MALDI revealed novelty of this protein while circular dichroism data confirmed its alpha helical nature. Higher dilutions of MIF-II antibody increased cauda sperm motility and induced immature/immotile caput sperm motility as tested microscopically. Higher number of sperm cells and lower dilutions of antibody induced agglutination in cauda sperm showing surface localization. Indirect immuno-fluorescence showed MIF-II localization throughout the caput sperm surface which relocated more towards acrosomal region with maturation. ELISA assay revealed gradual increase and decrease in concentration of MIF-II in epididymal plasma and plasma membrane respectively from caput to cauda. Signaling cascade that leads to sperm motility inhibition elevates nitric oxide levels through cAMP dependent pathway. MIF-II treatment doesn't alter sperm surface morphology. Expression pattern of MIF-II during epididymal maturation goes hand-in-hand with gaining motility potential as well as dormancy of spermatozoa before ejaculation. Both MIF-II and its antibody inhibit fertilization in-vitro thus expected to open new gateway for future male infertility and contraceptive development research.
Assuntos
Proteínas , Sistemas do Segundo Mensageiro/fisiologia , Motilidade dos Espermatozoides/fisiologia , Espermatozoides , Animais , AMP Cíclico/metabolismo , Epididimo/química , Epididimo/metabolismo , Humanos , Masculino , Proteínas/química , Proteínas/metabolismo , Coelhos , Ratos , Espermatozoides/química , Espermatozoides/metabolismoRESUMO
The presence of estrogen in the genital ducts of different mammalian species has been extensively studied and the estrogen influence on the functional activity of the male genital tract has been hypothesized. Conversely, very few data have been reported on pig excurrent ducts: the localization of classical estrogen receptors (ERα and ERß) is scarcely known, while the expression of the G protein-coupled receptor (GPER1), a membrane estrogen receptor, is still unknown in pig. The aim of the present study was to evaluate GPER1 expression in the different regions of the mature pig epididymis, using immunohistochemistry, western blot and RT-PCR analyses. The results showed that GPER1 is mainly expressed in the epithelial cells of the corpus epididymis compared to the caput and the cauda, while muscle cells are moderately immunostained and stromal cells are unstained. The presence of GPER1 was confirmed by Western blot and RT-PCR analyses. In our study, we have demonstrated for the first time the GPER1 expression in male porcine epididymis, revealing a new mediator of estrogen signaling at this site. In conclusion, these new data suggest that estrogen action via GPER1 may contribute to sperm maturation in the corpus and sperm protection/storage in the cauda. Interestingly, the presence of GPER1 in the muscle layer may be indicative of a possible GPER1 involvement in the estrogen regulation of duct contractility. Anat Rec, 2018. © 2018 Wiley Periodicals, Inc.
Assuntos
Epididimo/química , Epididimo/metabolismo , Receptores de Estrogênio/análise , Receptores de Estrogênio/biossíntese , Receptores Acoplados a Proteínas G/análise , Receptores Acoplados a Proteínas G/biossíntese , Animais , Masculino , SuínosRESUMO
OBJECTIVE: To study the effect of rutin on body weight and obesity-induced reproductive impairment in male mice. METHODS: Twenty-four male mice were randomized equally into normal control group, high-fat diet group (HFD group), and HFD + rutin intervention group (HRU group). After 28 days of treatments, the testes and epididymis of the mice were collected for detection of total cholesterol (TC) and triglycerides (TG) levels and for pathological examinations with HE staining. The expressions of related genes was detected with real-time PCR, and Western blotting was used to detect the expression of Ucp1 protein in the samples. RESULTS: After 28 days of treatments, the mean body weight was lower in mice with rutin intervention than in those in HFD group. The mice in HFD group showed significantly higher TG levels in the testis and epididymis and higher TC levels in the epididymis than those in the control and HRU groups. In HFD group, the testis and the epididymis displayed loosened structures with abnormalcell structure, and the number ofmature spermatozoa in the lumen was decreased and the mobility of the sperms was reduced; all these changes were significantly alleviated in HRU group. The expression levels of Ucp1 mRNA and protein increased (P<0.05) and the expressions of Mcp1 and TNF-α decreased significantly in the mice after rutin treatment (P<0.05). CONCLUSION: Rutin can effectively inhibit rapid increase of body weight and protect against obesity-induced reproductive impairment in obese mice.
Assuntos
Obesidade/fisiopatologia , Rutina/farmacologia , Disfunções Sexuais Fisiológicas/tratamento farmacológico , Animais , Peso Corporal , Quimiocina CCL2/metabolismo , Colesterol/análise , Dieta Hiperlipídica , Epididimo/química , Epididimo/patologia , Masculino , Camundongos , Camundongos Endogâmicos C57BL , Obesidade/complicações , Distribuição Aleatória , Disfunções Sexuais Fisiológicas/etiologia , Motilidade dos Espermatozoides , Testículo/química , Testículo/patologia , Triglicerídeos/análise , Fator de Necrose Tumoral alfa/metabolismo , Proteína Desacopladora 1/metabolismoRESUMO
This study evaluated the protective effects of 6-gingerol-rich fraction (6-GRF) from Zingiber officinale on carbendazim (CBZ)-induced reproductive toxicity in rats. Adult male rats were treated with either CBZ (50 mg/kg) alone or in combination with 6-GRF (50, 100 and 200 mg/kg) for 14 consecutive days. Gas chromatography-mass spectrometry (GCMS) analysis revealed that 6-GRF consists of ten bioactive chemical components with 6-gingerol being the most abundant (30.76%). Administration of 6-GRF significantly (p < .05) prevented CBZ-mediated increase in absolute and relative testes weights as well as restored the sperm quantity and quality in the treated rats to near control. In testes and epididymis, 6-GRF significantly abolished CBZ-mediated increase in oxidative damage as well as augmented antioxidant enzymes activities and glutathione level in the treated rats. Moreover, CBZ administration alone significantly decreased plasma levels of testosterone, thyrotropin, triiodothyronine and tetraiodothyronine, whereas follicle-stimulating hormone was significantly elevated without affecting luteinising hormone and prolactin levels when compared with the control. Conversely, 6-GRF ameliorated the disruption in the hormonal levels and restored their levels to near normalcy in CBZ-treated rats. Collectively, 6-GRF inhibited the adverse effects of CBZ on the antioxidant defence systems, hormonal balance and histology of the testes and epididymis in rats.
Assuntos
Benzimidazóis/toxicidade , Carbamatos/toxicidade , Catecóis/farmacologia , Disruptores Endócrinos , Epididimo/efeitos dos fármacos , Álcoois Graxos/farmacologia , Testículo/efeitos dos fármacos , Zingiber officinale/química , Animais , Catalase/metabolismo , Epididimo/química , Epididimo/patologia , Glutationa/análise , Peroxidação de Lipídeos/efeitos dos fármacos , Masculino , Tamanho do Órgão/efeitos dos fármacos , Ratos , Ratos Wistar , Contagem de Espermatozoides , Motilidade dos Espermatozoides/efeitos dos fármacos , Superóxido Dismutase/metabolismo , Testículo/química , Testículo/patologia , Aumento de Peso/efeitos dos fármacosRESUMO
Aquaporins (AQPs) are essential membrane protein channels for the transport of water across membranes. Fluid movement in the epididymis is important for modulation of the luminal environment, in which sperm mature and reside. This study was designed to understand the morphology and localization of AQPs in ram efferent ducts (ED) and epididymis. For this purpose, the epididymis of seven animals were removed for histologic and immunohistochemical analyses. AQP1 immunoreactivity was observed in the apex of the ED, and AQP9 was found adjacent to the nuclei of the epithelial cells of the ED. The epithelial lining of ram epididymis is pseudostratified columnar and presents principal, basal, apical and narrow cells. In the initial segment (IS), a moderate reaction for AQP1 was observed in the apical cytoplasm of epithelial cells. An intense reactivity for AQP1 was noted over the microvilli of principal cells and in spermatozoa in the caput. In the corpus and cauda, AQP1 was noted only over the endothelial cells of vascular channels located in intertubular spaces. A weak-to-moderate reaction for AQP9 was observed in the nuclei of epithelial cells in the IS, caput and corpus of the epididymis. In the cauda, an intense reaction to AQP9 was observed in the epithelial border. In the IS, caput and corpus, the reactivity for AQP9 differed from those observed in domestic animals. The cauda showed a pattern similar to that previously described. These results indicate that AQPs 1 and 9 have reversed locations and roles in rams, suggesting activity variations related with fluid and solute absorption throughout the epididymis.
Assuntos
Aquaporina 1/análise , Aquaporinas/análise , Epididimo/química , Ovinos , Animais , Células Epiteliais/química , Imuno-Histoquímica , Masculino , Testículo/químicaRESUMO
Caput epididymal wild-type spermatozoa and cauda epididymal spermatozoa from mice null for the adenylyl cyclase Adcy10 gene are immotile unless stimulated by a membrane-permeant cyclic AMP analogue. Both types of spermatozoa exhibit flagellar angulation where the head folds back under these conditions. As sperm proteins undergo oxidation of sulfhydryl groups and the flagellum becomes more stable to external forces during epididymal transit, we hypothesized that ADCY10 is involved in a mechanism regulating flagellar stabilization. Although no differences were observed in global sulfhydryl status between caput and cauda epididymal spermatozoa from wild-type or Adcy10-null mice, two-dimensional fluorescence difference gel electrophoresis was performed to identify specific mouse sperm proteins containing sulfhydryl groups that became oxidized during epididymal maturation. A-kinase anchor protein 4, fatty acid-binding protein 9 (FABP9), glutathione S-transferase mu 5 and voltage-dependent anion channel 2 exhibited changes in thiol status between caput and cauda epididymal spermatozoa. The level and thiol status of each of these proteins were quantified in wild-type and Adcy10-null cauda epididymal spermatozoa. No differences in the abundance of any protein were observed; however, FABP9 in Adcy10-null cauda epididymal spermatozoa contained fewer disulfide bonds than wild-type sperm cells. In caput epididymal spermatozoa, FABP9 was detected in the cytoplasmic droplet, principal piece, midpiece, and non-acrosomal area of the head. However, in cauda epididymal spermatozoa, this protein localized to the perforatorium, post-acrosomal region and principal piece. Together, these results suggest that thiol changes during epididymal maturation have a role in the stabilization of the sperm flagellum.
Assuntos
Adenilil Ciclases/genética , Epididimo/química , Flagelos/fisiologia , Espermatozoides/química , Compostos de Sulfidrila/química , Proteínas de Ancoragem à Quinase A/química , Animais , AMP Cíclico/análogos & derivados , AMP Cíclico/metabolismo , Dissulfetos/química , Epididimo/embriologia , Epididimo/crescimento & desenvolvimento , Proteínas de Ligação a Ácido Graxo/química , Glutationa Transferase/química , Infertilidade Masculina/metabolismo , Masculino , Camundongos , Camundongos Endogâmicos C57BL , Camundongos Knockout , Oxirredução , Espermatozoides/crescimento & desenvolvimento , Espermatozoides/metabolismo , Canal de Ânion 2 Dependente de Voltagem/químicaRESUMO
ESR2 is involved in oestrogen-related apoptosis in cell cycle spermatogenesis but their effects have not yet confirmed in pig. Therefore, this study was aimed to investigate the association of ESR2 polymorphism with sperm quality and boar fertility traits and to analyse the ESR2 mRNA and protein expressions in boar reproductive tissues. DNA samples from 203 Pietrain (PI) and 100 Pietrain × Hampshire (PIHA) pigs with records of sperm quality [sperm concentration (SCON), motility (MOT), semen volume (VOL), plasma droplet rate (PDR) and abnormal spermatozoa rate (ASR)] and fertility [non-return rate (NRR) and number of piglet born alive (NBA)] traits were available. A SNP in coding region of ESR2 g.35547A>G in exon 5 was associated with MOT and PDR in the PI and with SCON, VOL, MOT and PDR in PIHA population. For mRNA and protein expression study, a total of six boars were divided into two groups with group I (G-I) and group II (G-II) where G-I characterized for relatively a better sperm quality according to the mean of two groups. mRNA expression was higher in brain and testis than that in all parts of epididymis. Both qRT-PCR and western blot analysis revealed that the ESR2 gene expression and protein expression were significantly higher in testis collected from G-II compared with that of G-I boars. Moreover, ESR2 protein localization in germ cell, Leydig and Sertoli cells, epithelial cells and spermatozoa was remarkable, which indicated the important role of ESR2 in spermatogenesis process. These results might shed new light on the roles of ESR2 in spermatogenesis as candidate for boar fertility, but still the lack of association across populations should be considered.
Assuntos
Receptor beta de Estrogênio/genética , Fertilidade/genética , Fertilidade/fisiologia , Espermatozoides/fisiologia , Sus scrofa/genética , Sus scrofa/fisiologia , Animais , Química Encefálica , Epididimo/química , Receptor beta de Estrogênio/análise , Receptor beta de Estrogênio/fisiologia , Genótipo , Masculino , Reação em Cadeia da Polimerase/veterinária , RNA Mensageiro/análise , Contagem de Espermatozoides , Motilidade dos Espermatozoides , Espermatogênese/genética , Espermatogênese/fisiologia , Espermatozoides/anormalidades , Espermatozoides/química , Testículo/químicaAssuntos
Tumor Adenomatoide/diagnóstico , Epididimo/patologia , Tumor Adenomatoide/química , Tumor Adenomatoide/patologia , Adulto , Biópsia por Agulha Fina , Calbindina 2 , Núcleo Celular/química , Citoplasma/química , Epididimo/química , Humanos , Imuno-Histoquímica , Masculino , Mucina-1/química , Proteína G de Ligação ao Cálcio S100/químicaRESUMO
Epididymal lithiasis is a reproductive dysfunction of roosters that is associated with loss of fertility and is characterized by the formation of calcium stones in the lumen of the efferent ductules of the epididymal region. The efferent ductules of birds are responsible for the reabsorption of the fluid coming from the testis as well as luminal calcium. It has been hypothesized that the epididymal stone formation may be related to the impairment of local fluid or calcium homeostasis, which depends on hormones such as estradiol (E(2)). Therefore, this study aimed to investigate possible alterations in the expression of ERα (ESR1) and ERß (ESR2) in the epididymal region of roosters affected by epididymal lithiasis. The study was performed by immunohistochemistry and western blotting assays. In addition, the concentrations of E(2), vitamin D3, and testosterone, which are also key hormones in maintenance of calcium homeostasis, were determined in the plasma and epididymal region, by ELISA. It was observed that ESR2 expression is increased in all segments of the epididymal region of affected roosters, whereas ESR1 levels are not altered. Moreover, the hormone concentration profiles were changed, as in the epididymal region of roosters with lithiasis the E(2) levels were increased and vitamin D3 as well as testosterone concentrations were significantly decreased. These results suggest that a hormonal imbalance may be involved with the origin and progression of the epididymal lithiasis, possibly by affecting the local fluid or calcium homeostasis.
Assuntos
Galinhas , Colecalciferol/metabolismo , Estradiol/metabolismo , Receptor beta de Estrogênio/metabolismo , Doenças dos Genitais Masculinos/veterinária , Litíase/veterinária , Testosterona/metabolismo , Animais , Colecalciferol/análise , Epididimo/química , Epididimo/metabolismo , Epididimo/patologia , Estradiol/análise , Estradiol/sangue , Expressão Gênica , Doenças dos Genitais Masculinos/sangue , Doenças dos Genitais Masculinos/metabolismo , Doenças dos Genitais Masculinos/patologia , Imuno-Histoquímica , Litíase/sangue , Litíase/metabolismo , Litíase/patologia , Masculino , Modelos Biológicos , Doenças das Aves Domésticas/sangue , Doenças das Aves Domésticas/metabolismo , Doenças das Aves Domésticas/patologia , Testosterona/análise , Testosterona/sangueRESUMO
In order to assess the effect of obesity on epididymal and germinal epithelia in control rats and obese rats induced by a high fat diet, we evaluated the epididymal and testicular morphologies, lipid peroxidation in the epididymis, leptin serum levels, steroid hormones, insulin, cholesterol, triglycerides, glycemia and some spermatobioscopic parameters. No significant difference was observed in the levels of insulin, glucose, cholesterol and triglycerides between the two groups. Nonetheless, in the obese rats, circulating leptin and estradiol levels showed a significant increase and there was a decline in the testosterone levels. The same group showed an increase in the lipid peroxidation of the epididymis and reduced spermatobioscopic parameters. The heads of the epididymis showed morphological differences in obese rats. No significant difference was observed between the testes of both groups. There is a clear evidence of an effect on sperm in obese rats and this seems to occur in the epididymis.
Assuntos
Obesidade/patologia , Espermatozoides , Animais , Gorduras na Dieta/efeitos adversos , Modelos Animais de Doenças , Epididimo/química , Epididimo/efeitos dos fármacos , Epididimo/metabolismo , Epididimo/patologia , Células Epiteliais/química , Células Epiteliais/metabolismo , Células Epiteliais/patologia , Estradiol/sangue , Leptina/sangue , Peroxidação de Lipídeos/efeitos dos fármacos , Masculino , Obesidade/metabolismo , Obesidade/fisiopatologia , Ratos , Ratos Sprague-Dawley , Espermatozoides/efeitos dos fármacos , Testículo/química , Testículo/efeitos dos fármacos , Testículo/metabolismo , Testículo/patologia , Testosterona/sangueRESUMO
Several studies have been reported on the occurrence of sperm motility inhibiting factors in the male reproductive fluids of different mammalian species, but these proteins have not been adequately purified and characterized. A novel sperm motility inhibiting factor (MIF-II) has been purified from caprine epididymal plasma (EP) by Hydroxylapatite gel adsorption chromatography, DEAE-Cellulose ion-exchange chromatography and chromatofocusing. The MIF-II has been purified to apparent homogeneity and the molecular weight estimated by Sephacryl S-300 gel filtration is 160 kDa. MIF-II is a dimeric protein, made up of two subunits each having a molecular mass of 80 kDa as shown by SDS-PAGE. The isoelectric point of MIF-II is 5.1 as determined by chromatofocusing and isoelectric focusing. It is a heat labile protein and maximal active at the pH 6.9 to 7.5. The sperm motility inhibiting protein factor at 2 microg/ml (12.5 nM) level showed maximal motility-inhibiting activity. The observation that the epididymal plasma factor lowered the intracellular cAMP level of spermatozoa in a concentration-dependent manner suggests that it may block the motility of caprine cauda spermatozoa by interfering the cAMP dependent motility function. The results revealed that the purified protein factor has the potential of sperm motility inhibition and may serve as a vaginal contraceptive. The antibody raised against the MIF-II has the potential for enhancement of forward motility of cauda-spermatozoa. This antibody may thus be useful for solving some of the problems of male infertility due to low sperm motility.
Assuntos
Fracionamento Químico/métodos , Epididimo/química , Cabras , Motilidade dos Espermatozoides/efeitos dos fármacos , Animais , AMP Cíclico/metabolismo , Humanos , Espaço Intracelular/efeitos dos fármacos , Espaço Intracelular/metabolismo , Masculino , Análise do Sêmen , Espectrofotometria , Espermatozoides/citologia , Espermatozoides/efeitos dos fármacosRESUMO
The emerging insights into glycan functionality direct increasing attention to monitor core modifications of N-glycans and branch-end structures. To address this issue in histochemistry, a panel of lectins with respective specificities was devised. The selection of probes with overlapping specificities facilitated to relate staining profiles to likely target structures. The experiments on fixed sections of adult murine testis and epididymis were carried out at non-saturating lectin concentrations to visualize high-affinity sites with optimal signal-to-background ratio. They revealed selectivity in lectin reactivity for distinct cell types and segment-dependent staining in the epididymis. Leydig cells, for instance, were reactive with the Sambucus nigra agglutinin and human siglec-2 (CD22), two lectins also separating principal from basal and apical cells in the caput segments I-III of the epididymis. Apical cells were reactive with the Maackia amurensis agglutinin-I, and basal cells with the erythroagglutinin of Phaseolus vulgaris. The reported differences support the concept of lectin staining as cell marker. They thus intimate to study glycogene (genes for glycosyltransferases and lectins) expression and cellular reactivity with tissue lectins. These investigations will be instrumental to assign a role as biochemical signals to the detected staining properties.
Assuntos
Epididimo/química , Histocitoquímica/métodos , Lectinas , Polissacarídeos/análise , Testículo/química , Animais , Glicômica , Lectinas/química , Lectinas/metabolismo , Masculino , Camundongos , Camundongos Endogâmicos C57BL , Ácidos Nucleicos Peptídicos/metabolismo , Fito-Hemaglutininas/metabolismo , Lectinas de Plantas/metabolismo , Polissacarídeos/química , Polissacarídeos/metabolismo , Proteínas Ribossômicas/metabolismo , Proteínas Inativadoras de Ribossomos/metabolismo , Proteínas Inativadoras de Ribossomos Tipo 2 , Lectina 2 Semelhante a Ig de Ligação ao Ácido Siálico/metabolismo , Coloração e Rotulagem/métodos , Toxinas Biológicas/metabolismoRESUMO
The proteome of cauda epididymal fluid (CEF) from Holstein bulls was defined. Fluid was collected from the vas deferens, subjected to 2-D SDS-PAGE and spots identified by CapLC-MS/MS and MALDI-ToF/ToF. Because albumin accounted for 21.1% of all spot intensities in the gels examined by PDQuest, samples were subjected to albumin depletion and then analyzed again as before. Original CEF gels had 114 ± 3 spots, including as the most abundant: albumin, epididymal secretory protein E1, prostaglandin d-synthase and gelsolin. Epididymal fluid also expressed: clusterin, transferrin, N-acetyl-ß-glucosaminidase, cauxin, glutathione peroxidase, acidic seminal fluid protein (aSFP), aldehyde reductase, α-l-fucosidase, α-1-ß-glycoprotein, apolipoprotein A-1, ß actin, calmodulin, cathepsin D, cystatin E/M, enolase, galectin 3-binding protein, leucine amino-peptidase and nucleobindin. Albumin depletion decreased that very spot to 10% of its original intensity and the resulting gels had, on average, 137 ± 4 spots. Spots identified as dipeptidyl-peptidase 7, angiotensin-converting enzyme, arylsulfatase A, aspartylglucosaminidase, serine protease inhibitors, new isoforms of calmodulin, cystatin E/M and a 17-kDa nucleobindin appeared only in depleted maps. This study is the first to report nucleobindin and aSFP as epididymal components. We suggest that CEF proteins act to facilitate membrane remodeling, transport of lipophilic substances, protect sperm and prevent premature acrosome reaction.