RESUMO
Carrier-free pure drug self-assembled nanosystems have been proposed as a promising strategy for synergetic anticancer therapy. Herein, we purposefully designed and synthesized disulfide-modified glutathione (GSH)-responsive natural pentacyclic triterpene betulinic acid (BA) with better biodegradability and biocompatibility to construct carrier-free photosensitive prodrugs BA-S-S/Ce6 NPs for synergistically enhanced and biosafe photochemotherapy. The molecular dynamics simulation elucidates the possible coassembly mechanism that the coplanar arrangement of BA-S-S dimeric may be primarily responsible for the formation of a long lamella-like or spherical morphology. The density functional theory calculations demonstrate that the reduced energy gap (ΔEST) of Ce6 facilitates the improved singlet oxygen generation of BA-S-S/Ce6 nanoparticles (NPs). The assembled prodrugs exhibited remarkable GSH-responsive property and multiple favorable therapeutic features, leading to enhanced synergistic antitumor efficacy without noticeable toxicity. Additionally, evaluation of the antitumor efficacy of another tetracyclic triterpene stigmasterol (ST)-mediated ST-S-S/Ce6 NPs further confirmed the effectiveness of this rational design. This work provides a promising insight for exploring the pure drug self-assembly behavior and construction of GSH-responsive carrier-free triterpenoid prodrugs toward improved multiple combination antitumor therapies.
Assuntos
Antineoplásicos/uso terapêutico , Glutationa/metabolismo , Nanopartículas/uso terapêutico , Neoplasias/tratamento farmacológico , Triterpenos Pentacíclicos/uso terapêutico , Pró-Fármacos/uso terapêutico , Animais , Antineoplásicos/química , Antineoplásicos/metabolismo , Clorofilídeos , Teoria da Densidade Funcional , Sinergismo Farmacológico , Feminino , Luz , Camundongos Endogâmicos BALB C , Modelos Químicos , Simulação de Dinâmica Molecular , Nanopartículas/química , Triterpenos Pentacíclicos/química , Triterpenos Pentacíclicos/metabolismo , Fotoquimioterapia , Fármacos Fotossensibilizantes/química , Fármacos Fotossensibilizantes/uso terapêutico , Porfirinas/química , Porfirinas/uso terapêutico , Pró-Fármacos/química , Pró-Fármacos/metabolismo , Oxigênio Singlete/metabolismo , Estigmasterol/análogos & derivados , Estigmasterol/metabolismo , Estigmasterol/uso terapêutico , Ácido BetulínicoRESUMO
Withanolides constitute an extensive and vital class of metabolites displaying wide array of structural and therapeutic properties with unique side-chain modifications. These show diversified scaffolds and are promising pharmaceutical molecules with well documented anti-inflammatory and anti-cancer properties. Sterols are dynamic class of compounds and essential molecules having structural and functional significance. These contribute to the synthesis of withanolides by providing structural precursors. In this context, we have characterized sterol Δ22-desaturase from Withania somnifera and also functionally validating it by confirming its desaturase nature in conjunction with quantitative real-time expression profiling and metabolite evaluation. Further, transgenic hairy roots of W. somnifera displayed a higher accumulation of stigmasterol and withanolides. The increase in chemical constituents was concomitant with an increased gene copy number predicted via Southern blotting. Additionally, transgenic lines of tobacco over-expressing WsCYP710A11 displayed a substantial increase in its expression, corroborating well with enhanced stigmasterol content. Characterization of CYP710A11 from W. somnifera and its homologous transgenic expression has demonstrated its role in the regulation of withanolides biosynthesis. It also exhibited a differential transcriptional profile in response to exogenous elicitations. These empirical findings suggest the crucial role of CYP710A11 in stigmasterol biosynthesis. This in turn has implications for the overproduction of withanolides via pathway channelling.
Assuntos
Fitosteróis/metabolismo , Proteínas de Plantas/metabolismo , Estigmasterol/metabolismo , Withania/enzimologia , Vitanolídeos/metabolismo , Expressão Gênica , Oxirredutases/genética , Oxirredutases/metabolismo , Proteínas de Plantas/genética , Nicotiana/química , Nicotiana/enzimologia , Nicotiana/genética , Withania/química , Withania/genéticaRESUMO
Sterols are verified to be able to produce polycyclic aromatic hydrocarbons during its pyrolysis. In this study, a kind of Aspergillus fumigatus (LSD-1) was isolated from cigar leaves, and the biosorption effects on the stigmasterol, ß-sitosterol, campesterol, cholesterol, and ergosterol by using living and dead biomass of LSD-1 were investigated. The results showed that both living and dead biomass could efficiently remove these sterols in aqueous solution and tobacco waste extract (TWE). Interestingly, compared with the living biomass of LSD-1, the dead biomass of LSD-1 not only kept a high adsorption efficiency but also did not produce ergosterol. Overall, dead biomass of LSD-1 was a more suitable biosorbent to sterols in TWE. Furthermore, Brunner-Emmet-Teller (BET), Fourier transformed infrared spectrometer (FTIR) and scanning electron microscope (SEM) analysis were used to explore the biosorption process of living and dead biomass and their differences, suggesting that the biosorption of sterols was a physical process.
Assuntos
Absorção Fisiológica , Aspergillus fumigatus/metabolismo , Colesterol/análogos & derivados , Ergosterol/metabolismo , Nicotiana/química , Nicotiana/microbiologia , Fitosteróis/metabolismo , Extratos Vegetais/metabolismo , Sitosteroides/metabolismo , Estigmasterol/metabolismo , Biodegradação Ambiental , Biomassa , Colesterol/metabolismo , Concentração de Íons de Hidrogênio , Microscopia Eletrônica de Varredura , Folhas de Planta/química , Folhas de Planta/microbiologia , Espectroscopia de Infravermelho com Transformada de Fourier , Poluentes Químicos da Água/metabolismoRESUMO
Phytosterols are stucturally correlated to the endogenous ligands of Liver X Receptor (LXR), a ligand-activated nuclear receptor that has emerged as an attractive drug target due to its ability to integrate metabolic and inflammatory signaling. Natural and semi-synthetic phytosterol derivatives characterized by the presence of side-chain oxygenated functions have shown to be able to modulate LXR activity. Here, we describe the efficient synthesis of four stigmastane derivatives, endowed with a hydroxyl group at C24 position, namely (24R)- and (24S)-stigmasta-5,28-diene-3ß,24-ols (also referred to as saringosterols, 10a and 10b) and (24R)- and (24S)-stigmasta-5-ene-3ß,24-ols (11a and 11b), starting from the readily available stigmasterol. Thanks to X-ray crystallography the absolute configuration of the newly created chiral centers was definitively assigned for all the four compounds. The subsequent luciferase assays with GAL-4 chimeric receptors evidenced the ability of the two 24(S)-epimers, 10b and 11b, to interact with LXRs, showing the same degree of affinity as (22R)-hydroxycholesterol (1). With regard to the isoform selectivity both the derivatives 10b and 11b showed a preference for LXRß, up to 4-fold in terms of efficacy for 11b. The gene expression profiling of (24S)-stigmasta-5,28-diene-3ß,24-ol (10a) and (24S)-stigmasta-5-ene-3ß,24-ol (11a) demonstrated the capability of both the compounds to induce the expression of four well-known LXR target genes, such as ABCA1, SREBP1c, FASN, and SCD1 in U937 monocytic cell line, thus supporting the hypothesis they were LXR positive modulators.
Assuntos
Receptores X do Fígado/antagonistas & inibidores , Estigmasterol/análogos & derivados , Transportador 1 de Cassete de Ligação de ATP/genética , Transportador 1 de Cassete de Ligação de ATP/metabolismo , Linhagem Celular Tumoral , Cristalografia por Raios X , Humanos , Ligantes , Receptores X do Fígado/metabolismo , Conformação Molecular , Ligação Proteica , Isoformas de Proteínas/antagonistas & inibidores , Isoformas de Proteínas/metabolismo , Estereoisomerismo , Proteína de Ligação a Elemento Regulador de Esterol 1/genética , Proteína de Ligação a Elemento Regulador de Esterol 1/metabolismo , Estigmasterol/química , Estigmasterol/metabolismo , Estigmasterol/farmacologia , Transcriptoma/efeitos dos fármacosRESUMO
Chicories produce a wide range of vegetables with important nutritional value. We determined the variation of sterol, total polyphenol, nitrate contents and antioxidant capacity (SC, TPC, NC, AC) in endive leaves and stem-chicory novel vegetables, cultivated in two Italian regions. Within a given area, the SC was similar in smooth- and curly leafed endives (106.3-176.0 mg/kg FW); sitosterol and stigmasterol were major fractions (45-56 versus 38-43%). The stem SC was independent of landrace (101.5-118.6 mg/kg FW); sitosterol prevailed on stigmasterol and fucosterol (73-76 versus 12-14% versus 8-9%); the latter reached 15.7 mg/kg FW, conferring value as potential antidiabetes food. The planting site affected the AC and TPC of endives (893.1-1571.4 µmTE/100 g FW, 30.8-76.1 GAE100/g FW) and chicory stems (729.8-1152.5 µmTE/100 g FW; 56.2-124.4 GAE100/g FW), while the NC was recurrently below dangerous thresholds. PCA showed that environment was the major cause of variation, though it modestly affected these parameters.
Assuntos
Antioxidantes/análise , Asteraceae/química , Cichorium intybus/química , Produtos Agrícolas/química , Fitosteróis/análise , Folhas de Planta/química , Caules de Planta/química , Antioxidantes/química , Antioxidantes/metabolismo , Asteraceae/crescimento & desenvolvimento , Asteraceae/metabolismo , Cichorium intybus/crescimento & desenvolvimento , Cichorium intybus/metabolismo , Produção Agrícola , Produtos Agrícolas/crescimento & desenvolvimento , Produtos Agrícolas/metabolismo , Contaminação de Alimentos , Alimento Funcional/análise , Humanos , Itália , Nitratos/análise , Nitratos/química , Nitratos/metabolismo , Valor Nutritivo , Capacidade de Absorbância de Radicais de Oxigênio , Fenóis/análise , Fenóis/química , Fenóis/metabolismo , Fitosteróis/biossíntese , Fitosteróis/química , Folhas de Planta/crescimento & desenvolvimento , Folhas de Planta/metabolismo , Caules de Planta/crescimento & desenvolvimento , Caules de Planta/metabolismo , Análise de Componente Principal , Sitosteroides/análise , Sitosteroides/química , Sitosteroides/metabolismo , Análise Espaço-Temporal , Especificidade da Espécie , Estigmasterol/análogos & derivados , Estigmasterol/análise , Estigmasterol/química , Estigmasterol/metabolismoRESUMO
Nicotiana benthamiana was used as a model to investigate the spatial and developmental relationship between sterol synthesis rates and sterol content in plants. Stigmasterol levels were approximately twice the level in roots as that found in aerial tissues, while its progenitor sterol sitosterol was the inverse. When incorporation of radiolabeled precursors into sterols was used as measure of in vivo synthesis rates, acetate incorporation was similar across all tissue types, but approximately twofold greater in roots than any other tissue. In contrast, mevalonate incorporation exhibited the greatest differential with the rate of incorporation in roots approximately one-tenth that in apical shoots. Similar to acetate, incorporation of farnesol was higher in roots but remained fairly constant in aerial tissues, suggesting less regulation of the downstream sterol biosynthetic steps. Consistent with the precursor incorporation data, analysis of gene transcript and measurements of putative rate-limiting enzyme activities for 3-hydroxy-3-methylglutaryl-coenzyme A synthase (EC 2.3.3.10) and reductase (EC 1.1.1.34) showed the greatest modulation of levels, while the activity levels for isopentenyl diphosphate isomerase (EC 5.3.3.2) and prenyltransferases (EC 2.5.1.10 and EC 2.5.1.1) also exhibited a strong but moderate correlation with the development age of the aerial tissues of the plants. Overall, the data suggest a multitude of means from transcriptional to posttranslational control affecting sterol biosynthesis and accumulation across an entire plant, and point to some particular control points that might be manipulated using molecular genetic approaches to better probe the role of sterols in plant growth and development.
Assuntos
Nicotiana/metabolismo , Fitosteróis/metabolismo , Sitosteroides/metabolismo , Estigmasterol/metabolismo , Vias Biossintéticas , Ácido Mevalônico/metabolismo , Especificidade de Órgãos , Fitosteróis/química , Raízes de Plantas/genética , Raízes de Plantas/crescimento & desenvolvimento , Raízes de Plantas/metabolismo , Sitosteroides/química , Estigmasterol/química , Nicotiana/genética , Nicotiana/crescimento & desenvolvimentoRESUMO
Withanolides biosynthesis in the plant Withania somnifera (L.) Dunal is hypothesized to be diverged from sterol pathway at the level of 24-methylene cholesterol. The conversion and translocation of intermediates for sterols and withanolides are yet to be characterized in this plant. To understand the influence of mevalonate (MVA) and 2-C-methyl-d-erythritol-4-phosphate (MEP) pathways on sterols and withanolides biosynthesis in planta, we overexpressed the WsHMGR2 and WsDXR2 in tobacco, analyzed the effect of transient suppression through RNAi, inhibited MVA and MEP pathways and fed the leaf tissue with different sterols. Overexpression of WsHMGR2 increased cycloartenol, sitosterol, stigmasterol and campesterol compared to WsDXR2 transgene lines. Increase in cholesterol was, however, marginally higher in WsDXR2 transgenic lines. This was further validated through transient suppression analysis, and pathway inhibition where cholesterol reduction was found higher due to WsDXR2 suppression and all other sterols were affected predominantly by WsHMGR2 suppression in leaf. The transcript abundance and enzyme analysis data also correlate with sterol accumulation. Cholesterol feeding did not increase the withanolide content compared to cycloartenol, sitosterol, stigmasterol and campesterol. Hence, a preferential translocation of carbon from MVA and MEP pathways was found differentiating the sterols types. Overall results suggested that MVA pathway was predominant in contributing intermediates for withanolides synthesis mainly through the campesterol/stigmasterol route in planta.
Assuntos
Colesterol/análogos & derivados , Eritritol/análogos & derivados , Ácido Mevalônico/metabolismo , Nicotiana/enzimologia , Fitosteróis/metabolismo , Fosfatos Açúcares/metabolismo , Withania/enzimologia , Vitanolídeos/metabolismo , Sequência de Bases , Vias Biossintéticas , Carbono/metabolismo , Colesterol/química , Colesterol/metabolismo , Eritritol/metabolismo , Expressão Gênica , Regulação da Expressão Gênica de Plantas , Ácido Mevalônico/química , Dados de Sequência Molecular , Filogenia , Fitosteróis/química , Folhas de Planta/química , Folhas de Planta/enzimologia , Folhas de Planta/genética , Proteínas de Plantas/genética , Proteínas de Plantas/metabolismo , Plantas Geneticamente Modificadas , Análise de Sequência de DNA , Sitosteroides/química , Sitosteroides/metabolismo , Esteróis/química , Esteróis/metabolismo , Estigmasterol/química , Estigmasterol/metabolismo , Nicotiana/genética , Triterpenos/química , Triterpenos/metabolismo , Withania/química , Withania/genética , Vitanolídeos/químicaRESUMO
The replication of plus-strand RNA viruses depends on subcellular membranes. Recent genome-wide screens have revealed that the sterol biosynthesis genes ERG25 and ERG4 affected the replication of Tomato bushy stunt virus (TBSV) in a yeast model host. To further our understanding of the role of sterols in TBSV replication, we demonstrate that the downregulation of ERG25 or the inhibition of the activity of Erg25p with an inhibitor (6-amino-2-n-pentylthiobenzothiazole; APB) leads to a 3- to 5-fold reduction in TBSV replication in yeast. In addition, the sterol biosynthesis inhibitor lovastatin reduced TBSV replication by 4-fold, confirming the importance of sterols in viral replication. We also show reduced stability for the p92(pol) viral replication protein as well as a decrease in the in vitro activity of the tombusvirus replicase when isolated from APB-treated yeast. Moreover, APB treatment inhibits TBSV RNA accumulation in plant protoplasts and in Nicotiana benthamiana leaves. The inhibitory effect of APB on TBSV replication can be complemented by exogenous stigmasterol, the main plant sterol, suggesting that sterols are required for TBSV replication. The silencing of SMO1 and SMO2 genes, which are orthologs of ERG25, in N. benthamiana reduced TBSV RNA accumulation but had a lesser inhibitory effect on the unrelated Tobacco mosaic virus, suggesting that various viruses show different levels of dependence on sterol biosynthesis for their replication.
Assuntos
Nicotiana/metabolismo , Nicotiana/virologia , Esteróis/biossíntese , Tombusvirus/fisiologia , Replicação Viral/fisiologia , Colesterol/análogos & derivados , Colesterol/metabolismo , Inibidores Enzimáticos/metabolismo , Inativação Gênica , Inibidores de Hidroximetilglutaril-CoA Redutases/metabolismo , Lovastatina/metabolismo , Oxigenases de Função Mista/genética , Oxigenases de Função Mista/metabolismo , Oxirredutases/genética , Oxirredutases/metabolismo , Fitosteróis/metabolismo , Proteínas de Plantas/genética , Proteínas de Plantas/metabolismo , Protoplastos/metabolismo , RNA Viral/genética , RNA Viral/metabolismo , RNA Polimerase Dependente de RNA/genética , RNA Polimerase Dependente de RNA/metabolismo , Saccharomyces cerevisiae/genética , Saccharomyces cerevisiae/metabolismo , Saccharomyces cerevisiae/virologia , Proteínas de Saccharomyces cerevisiae/genética , Proteínas de Saccharomyces cerevisiae/metabolismo , Estigmasterol/metabolismo , Tiazóis/metabolismo , Nicotiana/citologia , Tombusvirus/genética , Proteínas Virais/genética , Proteínas Virais/metabolismoRESUMO
A large body of evidence from the past decade supports the existence of functional microdomains in membranes of animal and yeast cells, which play important roles in protein sorting, signal transduction, or infection by pathogens. They are based on the dynamic clustering of sphingolipids and cholesterol or ergosterol and are characterized by their insolubility, at low temperature, in nonionic detergents. Here we show that similar microdomains also exist in plant plasma membrane isolated from both tobacco leaves and BY2 cells. Tobacco lipid rafts were found to be greatly enriched in a sphingolipid, identified as glycosylceramide, as well as in a mixture of stigmasterol, sitosterol, 24-methylcholesterol, and cholesterol. Phospho- and glycoglycerolipids of the plasma membrane were largely excluded from lipid rafts. Membrane proteins were separated by one- and two-dimensional gel electrophoresis and identified by tandem mass spectrometry or use of specific antibody. The data clearly indicate that tobacco microdomains are able to recruit a specific set of the plasma membrane proteins and exclude others. We demonstrate the recruitment of the NADPH oxidase after elicitation by cryptogein and the presence of the small G protein NtRac5, a negative regulator of NADPH oxidase, in lipid rafts.
Assuntos
Colesterol/análogos & derivados , Detergentes/farmacologia , Microdomínios da Membrana/química , Nicotiana/metabolismo , Octoxinol/farmacologia , Fitosteróis , Western Blotting , Membrana Celular/metabolismo , Centrifugação com Gradiente de Concentração , Colesterol/metabolismo , Cromatografia Líquida de Alta Pressão , Cromatografia em Camada Fina , Eletroforese em Gel Bidimensional , Eletroforese em Gel de Poliacrilamida , Ergosterol/metabolismo , Íons , Metabolismo dos Lipídeos , Lipídeos/química , Espectrometria de Massas , Microdomínios da Membrana/metabolismo , Microscopia Eletrônica , NADPH Oxidases/metabolismo , Folhas de Planta/metabolismo , Estrutura Terciária de Proteína , Transdução de Sinais , Sitosteroides/metabolismo , Espectrometria de Massas por Ionização e Dessorção a Laser Assistida por Matriz , Estigmasterol/metabolismo , Sacarose/farmacologia , TemperaturaRESUMO
A study was conducted to analyze the effect of the antioxidants butylated hydroxytoluene, alpha-tocopherol, ethanolic extracts of rosemary, and green tea on stigmasterol resistance against degradation and formation of its oxidation products in purified triacylglycerols (TAG) from sunflower oil. The content of stigmasterol and its oxidation products 7alpha- and 7beta-hydroxy, alpha- and beta-epoxy, triol, and 7-ketostigmasterol were determined during incubation at 60 degrees C for 3, 6, and 9 days. In addition, peroxide value and fatty acid composition were also determined in the samples. Correlation between the levels of the accumulated stigmasterol oxides and peroxide value of the TAG with antioxidants during incubation was significant only for rosemary extract (R = 0.6799, p < 0.05). The lack of correlation precludes the use of peroxide values to determine the level of sterol oxidation products in the used model system. Correlation between stigmasterol content and the level of stigmasterol oxides was significant for all samples (R = 0.8874, p < 0.05). The total increase of the stigmasterol oxidation products was the lowest in samples with alpha-tocopherol, but the content of stigmasterol-triol increased the most in this sample. In all the analyzed samples, alpha-epoxy-stigmasterol was formed in the highest amounts among the analyzed stigmasterol oxidation products.
Assuntos
Antioxidantes/farmacologia , Óleos de Plantas/metabolismo , Estigmasterol/metabolismo , Oxirredução , Óleo de GirassolRESUMO
The first committed step in the conversion of cycloartenol into Delta(5) C24-alkyl sterols in plants is catalyzed by an S-adenosyl-methionine-dependent sterol-C24-methyltransferase type 1 (SMT1). We report the consequences of overexpressing SMT1 in tobacco (Nicotiana tabacum), under control of either the constitutive carnation etched ring virus promoter or the seed-specific Brassica napus acyl-carrier protein promoter, on sterol biosynthesis in seed tissue. Overexpression of SMT1 with either promoter increased the amount of total sterols in seed tissue by up to 44%. The sterol composition was also perturbed with levels of sitosterol increased by up to 50% and levels of isofucosterol and campesterol increased by up to 80%, whereas levels of cycloartenol and cholesterol were decreased by up to 53% and 34%, respectively. Concomitant with the enhanced SMT1 activity was an increase in endogenous 3-hydroxy-3-methylglutaryl coenzyme A reductase activity, from which one can speculate that reduced levels of cycloartenol feed back to up-regulate 3-hydroxy-3-methylglutaryl coenzyme A reductase activity and thereby control the carbon flux into sterol biosynthesis. This potential regulatory role of SMT1 in seed sterol biosynthesis is discussed.
Assuntos
Carbono/metabolismo , Colesterol/análogos & derivados , Metiltransferases/metabolismo , Nicotiana/enzimologia , Fitosteróis/biossíntese , Estigmasterol/análogos & derivados , Transporte Biológico , Colesterol/metabolismo , Clonagem Molecular , Regulação Enzimológica da Expressão Gênica , Regulação da Expressão Gênica de Plantas , Hidroximetilglutaril-CoA Redutases/metabolismo , Metiltransferases/genética , Fitosteróis/química , Fitosteróis/metabolismo , Folhas de Planta/metabolismo , Plantas Geneticamente Modificadas , Sementes/metabolismo , Sitosteroides/metabolismo , Estigmasterol/metabolismo , Nicotiana/genética , Nicotiana/metabolismo , TriterpenosRESUMO
Sterols are essential nutrients for all arthropods, including grasshoppers, but metabolic constraints may limit which sterols can support normal growth and development. In the firsts part of this study, a comparative experiment, which included five different species of grasshoppers (Orthoptera: Acrididae) representing three separate taxonomic groups, was performed to determine how widespread sterol metabolic constraints are within the Acrididae. Grasshoppers were reared on artificial diets containing sterols that differed in the position of double bonds within the sterol structure, and various life history traits were measured. Sterols with double bonds at position 7, within the sterol nucleus, and/or at position 22, on the cholestane side chain, failed to support development to the adult stage for any of the five species. In addition, grasshoppers reared on sterols with these configurations often had extended developmental times and reduced growth rates in the first and second stadium compared with grasshoppers reared on sitosterol or cholesterol diets. In the second half of this study, we examined how mixtures of suitable and unsuitable sterols influenced survival, growth, and development. Artificial foods containing mixtures of suitable and unsuitable sterols were fed to the highly polyphagous grasshopper Schistocerca americana. Results suggest that survival and performance of this grasshopper suffer as the concentration of unsuitable sterols increases and as the ratio of suitable to unsuitable sterols in the diet decreases. We review the literature to document variation in plant sterol profiles and propose that constraints on sterol metabolism may contribute to the maintenance of diet mixing in the Acrididae.
Assuntos
Colesterol/metabolismo , Gafanhotos/metabolismo , Fitosteróis/metabolismo , Animais , Colesterol/fisiologia , Cromatografia Líquida de Alta Pressão/veterinária , Feminino , Gafanhotos/crescimento & desenvolvimento , Gafanhotos/fisiologia , Muda/fisiologia , Necessidades Nutricionais , Sitosteroides/metabolismo , Estatísticas não Paramétricas , Estigmasterol/análogos & derivados , Estigmasterol/metabolismoRESUMO
To investigate the metabolism and possible deleterious effects of 4-methyl and 4,4-dimethyl steroids in Manduca sexta, the 4,4-dimethyl sterols lanosterol and cycloartenol, the 4-methyl sterol obtusifoliol and the 4,4-dimethyl pentacyclic triterpenoid alpha-amyrin were fed in an artificial agar-based diet at various concentrations. Utilization and metabolism of these four compounds were compared with sitosterol, stigmasterol, brassicasterol, ergosterol and 24-methylenecholesterol, 24-alkyl sterols that are readily dealkylated and converted to cholesterol in Manduca and in most phytophagous insects. None of the 4-methylated compounds significantly inhibited development except at very high dietary concentrations. The delta 24-bonds of lanosterol and cycloartenol were effectively reduced by the Manduca delta 24-sterol reductase enzyme, as is the delta 24-bond of desmosterol which, in most phytophagous insects, is an intermediate in the conversion of sitosterol, stigmasterol and other C28 and C29 phytosterols to cholesterol. On the other hand, the 24-methylene substituent of obtusifoliol was not dealkylated. Each of the 4-desmethyl C28 and C29 sterols was readily converted to cholesterol, and a significant amount of 7-dehydrocholesterol was derived from ergosterol metabolism. The reason for the differences in substrate specificity of these sterols is not clear, but the information may be useful in the development of new, specific, mechanism-based inhibitors of sterol metabolism.
Assuntos
Manduca/metabolismo , Esteróis/metabolismo , Animais , Colestadienóis/metabolismo , Colesterol/análogos & derivados , Colesterol/metabolismo , Ergosterol/metabolismo , Fitosteróis , Sitosteroides/metabolismo , Estigmasterol/metabolismoRESUMO
The binding of the membrane-active lipopeptide antibiotic iturin A to yeast cells was studied using radioactive iturin A. Saccharomyces cerevisiae had a maximum binding capacity of 5.6 x 10(9) molecules per single cell. The Scatchard plot of binding showed a biphasic profile, with a lower dissociation constant for small concentrations of iturin A. The break of slope at 30 microM iturin A corresponds to the micellization of antibiotic in solution. The binding is also dependent on the nature of the sterol present in the membrane. A mutant yeast strain with a membrane containing cholesterol instead of ergosterol showed the highest affinity for iturin A and the highest sensitivity to this antibiotic, as measured by K+ ion release. In contrast the presence of stigmasterol increased the resistance of the cells to iturin A.
Assuntos
Antibacterianos , Antifúngicos/farmacologia , Colesterol/metabolismo , Peptídeos , Fitosteróis/metabolismo , Saccharomyces cerevisiae/efeitos dos fármacos , Estigmasterol/metabolismo , Membrana Celular/análise , Membrana Celular/metabolismo , Permeabilidade da Membrana Celular/efeitos dos fármacos , Resistência Microbiana a Medicamentos , Peptídeos Cíclicos/metabolismo , Peptídeos Cíclicos/farmacologia , Potássio/metabolismo , Saccharomyces cerevisiae/citologia , Saccharomyces cerevisiae/metabolismo , Esferoplastos/metabolismoRESUMO
A novel pro-insecticide, 29-fluorostigmasterol, is proposed to cause mortality due to release of fluoroacetate during side chain dealkylation. The 29-3H-labeled substrate was fed to third instar tobacco hornworms (Manduca sexta) and erythro-2-fluoro-[2-3H] citrate was isolated in 0.012% yield by ion-exchange, silica gel, and reverse-phase chromatography of the tricarboxylic acid, trimethyl ester, and trimethyl ester benzoate, respectively. The less toxic 29-fluoro-[29-3H]sitosterol did not provide sufficient labeled fluorocitrate to allow isolation, while a more toxic 16-3H-labeled 16-fluorofatty acid gave nearly 1% conversion to labeled fluorocitrate. This is the first direct chemical evidence for the fate of the two carbons removed during phytosterol dealkylation in an insect. It is also the first use of labeled fluoroacetate precursors to identify labeled 2-fluorocitrate as an in vivo metabolite of these precursors.
Assuntos
Citratos/isolamento & purificação , Lepidópteros/metabolismo , Mariposas/metabolismo , Fitosteróis/metabolismo , Estigmasterol/metabolismo , Cromatografia Líquida de Alta Pressão , Citratos/biossíntese , Larva/metabolismo , Espectroscopia de Ressonância Magnética , Estigmasterol/análogos & derivados , TrítioRESUMO
The steroids which have been isolated from plant callus and suspension cultures are reviewed. In addition, the research involving the use of plant tissue cultures to investigate steroid biosynthesis and metabolism is summarized.