RESUMO
To improve the crop yield and quality, the cytosolic fructose-1,6-bisphosphatase (cFBPase) from mung bean (Vigna radiata), a rate-limiting enzyme in gluconeogenesis, was cloned, purified, and structurally characterised. To function it required Mg2+ and Mn2+ at 0.01-10 mM. The Michaelis-Menton constant and adenosine monophosphate (AMP) inhibitory constant (Ki) were 7.96 and 111.09 µM, respectively. The functional site residues of AMP binding (Arg30, Asp32, and Phe33) and the active site residues (Asn218 and Met251) were tested via site-directed mutagenesis and molecular docking. Asn218 and Met251 were replaced by Tyr and Leu, respectively. The M251L mutant showed enhanced substrate affinity and activity, resulting from decreased binding energy (-2.58 kcal·mol-1) and molecular distance (4.2 Å). AMP binding site mutations changed the enzyme activities, indicating a connection between the binding and active sites. Furthermore, Ki and docking analysis revealed that Asp32 plays a key role in maintaining the AMP binding conformation.
Assuntos
Citosol/enzimologia , Frutose-Bifosfatase/genética , Frutose-Bifosfatase/isolamento & purificação , Vigna/enzimologia , Vigna/genética , Monofosfato de Adenosina/metabolismo , Animais , Sítios de Ligação , Clonagem Molecular , Frutose-Bifosfatase/química , Frutose-Bifosfatase/metabolismo , Cinética , Simulação de Acoplamento Molecular , Mutagênese Sítio-Dirigida , Vigna/citologiaRESUMO
Serum-soluble folate binding protein (FBP) is an important tumor marker, and the development of a simple biosensing method is highly needed. In this work, a photoelectrochemical (PEC) biosensor for the detection of FBP was proposed based on the construction of an antifouling interface and the unique ligand-protein recognition. The PEC sensing platform was prepared by the biomimetic polydopamine (PDA) coating on TiO2 nanotubes arrays (NTAs). A significant PEC enhancement effect was obtained due to the macroporous structures. Excellent antifouling performance was achieved by conjugation of amino-group-terminated 8-arm poly(ethylene glycol) (PEG). The incorporation of folic acid (FA) retains the antifouling property and shows recognition abilities toward FBP. The fabricated PEC biosensor shows good analytical performance. The combination of ligand-protein recognition and a PEC antifouling interface provides a good consideration for the development of FBP biosensors.
Assuntos
Biomarcadores Tumorais/isolamento & purificação , Técnicas Biossensoriais , Neoplasias/sangue , Biomarcadores Tumorais/sangue , Ácido Fólico/química , Ácido Fólico/farmacologia , Frutose-Bifosfatase/sangue , Frutose-Bifosfatase/isolamento & purificação , Humanos , Indóis/química , Nanotubos/química , Neoplasias/patologia , Processos Fotoquímicos , Polietilenoglicóis/química , Polímeros/química , Titânio/químicaRESUMO
To enhance our understanding of the control of archaeal carbon central metabolism, a detailed analysis of the regulation mechanisms of both fructose1,6-bisphosphatase (FruBPase) and ADP-phosphofructokinase-1 (ADP-PFK1) was carried out in the methanogen Methanosarcina acetivorans. No correlations were found among the transcript levels of the MA_1152 and MA_3563 (frubpase type II and pfk1) genes, the FruBPase and ADP-PFK1 activities, and their protein contents. The kinetics of the recombinant FruBPase II and ADP-PFK1 were hyperbolic and showed simple mixed-type inhibition by AMP and ATP, respectively. Under physiological metabolite concentrations, the FruBPase II and ADP-PFK1 activities were strongly modulated by their inhibitors. To assess whether these enzymes were also regulated by a phosphorylation/dephosphorylation process, the recombinant enzymes and cytosolic-enriched fractions were incubated in the presence of commercial protein phosphatase or protein kinase. De-phosphorylation of ADP-PFK1 slightly decreased its activity (i.e. Vmax) and did not change its kinetic parameters and oligomeric state. Thus, the data indicated a predominant metabolic regulation of both FruBPase and ADP-PFK1 activities by adenine nucleotides and suggested high degrees of control on the respective pathway fluxes.
Assuntos
Proteínas Arqueais/metabolismo , Frutose-Bifosfatase/metabolismo , Methanosarcina/metabolismo , Fosfofrutoquinase-1/metabolismo , Difosfato de Adenosina/metabolismo , Monofosfato de Adenosina/metabolismo , Trifosfato de Adenosina/metabolismo , Animais , Proteínas Arqueais/genética , Proteínas Arqueais/isolamento & purificação , Galinhas , Frutose-Bifosfatase/genética , Frutose-Bifosfatase/isolamento & purificação , Frutosefosfatos/metabolismo , Genes Arqueais , Cinética , Methanosarcina/genética , Fosfofrutoquinase-1/genética , Fosfofrutoquinase-1/isolamento & purificação , Fosforilação , Inibidores de Proteínas Quinases/metabolismo , Processamento de Proteína Pós-TraducionalRESUMO
Cell-free biosystems comprised of synthetic enzymatic pathways would be a promising biomanufacturing platform due to several advantages, such as high product yield, fast reaction rate, easy control and access, and so on. However, it was essential to produce (purified) enzymes at low costs and stabilize them for a long time so to decrease biocatalyst costs. We studied the stability of the four recombinant enzyme mixtures, all of which originated from thermophilic microorganisms: triosephosphate isomerase (TIM) from Thermus thermophiles, fructose bisphosphate aldolase (ALD) from Thermotoga maritima, fructose bisphosphatase (FBP) from T. maritima, and phosphoglucose isomerase (PGI) from Clostridium thermocellum. It was found that TIM and ALD were very stable at evaluated temperature so that they were purified by heat precipitation followed by gradient ammonia sulfate precipitation. In contrast, PGI was not stable enough for heat treatment. In addition, the stability of a low concentration PGI was enhanced by more than 25 times in the presence of 20 mg/L bovine serum albumin or the other three enzymes. At a practical enzyme loading of 1000 U/L for each enzyme, the half-life time of free PGI was prolong to 433 h in the presence of the other three enzymes, resulting in a great increase in the total turn-over number of PGI to 6.2×10(9) mole of product per mole of enzyme. This study clearly suggested that the presence of other proteins had a strong synergetic effect on the stabilization of the thermolabile enzyme PGI due to in vitro macromolecular crowding effect. Also, this result could be used to explain why not all enzymes isolated from thermophilic microorganisms are stable in vitro because of a lack of the macromolecular crowding environment.
Assuntos
Proteínas de Bactérias/isolamento & purificação , Frutose-Bifosfatase/isolamento & purificação , Frutose-Bifosfato Aldolase/isolamento & purificação , Glucose-6-Fosfato Isomerase/isolamento & purificação , Triose-Fosfato Isomerase/isolamento & purificação , Proteínas de Bactérias/química , Biocatálise , Clostridium thermocellum/química , Clostridium thermocellum/enzimologia , Ensaios Enzimáticos , Estabilidade Enzimática , Frutose-Bifosfatase/química , Frutose-Bifosfato Aldolase/química , Glucose-6-Fosfato Isomerase/química , Meia-Vida , Cinética , Soroalbumina Bovina/química , Temperatura , Thermotoga maritima/química , Thermotoga maritima/enzimologia , Thermus thermophilus/química , Thermus thermophilus/enzimologia , Triose-Fosfato Isomerase/químicaRESUMO
To understand the physiological functions of thermostable fructose-1,6-bisphosphatase (TNA1-Fbp) from Thermococcus onnurineus NA1, its recombinant enzyme was overexpressed in Escherichia coli, purified, and the enzymatic properties were characterized. The enzyme showed maximal activity for fructose-1,6-bisphosphate at 95°C and pH 8.0 with a half-life (t (1/2)) of about 8 h. TNA1-Fbp had broad substrate specificities for fructose-1,6-bisphosphate and its analogues including fructose-1-phosphate, glucose-1-phosphate, and phosphoenolpyruvate. In addition, its enzyme activity was increased five-fold by addition of 1 mM Mg(2+), while Li(+) did not enhance enzymatic activity. TNA1-Fbp activity was inhibited by ATP, ADP, and phosphoenolpyruvate, but AMP up to 100 mM did not have any effect. TNA1-Fbp is currently defined as a class V fructose-1,6-bisphosphatase (FBPase) because it is very similar to FBPase of Thermococcus kodakaraensis KOD1 based on sequence homology. However, this enzyme shows a different range of substrate specificities. These results suggest that TNA1-Fbp can establish new criterion for class V FBPases.
Assuntos
Frutose-Bifosfatase/metabolismo , Thermococcus/enzimologia , Difosfato de Adenosina/metabolismo , Trifosfato de Adenosina/metabolismo , Clonagem Molecular , Ativadores de Enzimas/metabolismo , Inibidores Enzimáticos/metabolismo , Escherichia coli/genética , Frutose-Bifosfatase/química , Frutose-Bifosfatase/genética , Frutose-Bifosfatase/isolamento & purificação , Frutosefosfatos/metabolismo , Expressão Gênica , Glucofosfatos/metabolismo , Meia-Vida , Temperatura Alta , Concentração de Íons de Hidrogênio , Magnésio/metabolismo , Fosfoenolpiruvato/metabolismo , Proteínas Recombinantes/química , Proteínas Recombinantes/genética , Proteínas Recombinantes/isolamento & purificação , Proteínas Recombinantes/metabolismo , Especificidade por SubstratoRESUMO
A mid-infrared enzymatic assay for label-free monitoring of the enzymatic reaction of fructose-1,6-bisphosphatase with fructose 1,6-bisphosphate has been proposed. The whole procedure was done in an automated way operating in the stopped flow mode by incorporating a temperature-controlled flow cell in a sequential injection manifold. Fourier transform infrared difference spectra were evaluated for kinetic parameters, like the Michaelis-Menten constant (K(M)) of the enzyme and Vmax of the reaction. The obtained K(M) of the reaction was 14 +/- 3 g L(-1) (41 microM). Furthermore, inhibition by adenosine 5'-monophosphate (AMP) was evaluated, and the K(M)(App) value was determined to be 12 +/- 2 g L(-1) (35 microM) for 7.5 and 15 microM AMP, respectively, with Vmax decreasing from 0.1 +/- 0.03 to 0.05 +/- 0.01 g L(-1) min(-1). Therefore, AMP exerted a non-competitive inhibition.
Assuntos
Monofosfato de Adenosina/metabolismo , Análise de Injeção de Fluxo/instrumentação , Frutose-Bifosfatase/antagonistas & inibidores , Frutose-Bifosfatase/metabolismo , Espectroscopia de Infravermelho com Transformada de Fourier/métodos , Desenho de Equipamento , Frutose-Bifosfatase/isolamento & purificação , Frutosedifosfatos/metabolismo , Humanos , Cinética , Fígado/enzimologia , Espectroscopia de Infravermelho com Transformada de Fourier/instrumentaçãoRESUMO
It was shown for the first time that extracellular FBPase of B. subtilis 668 like the preparation obtained from culture liquid of B. subtilis B 7025 displays citotoxicity activity in respect of tumor cells of sarcoma 37 in vitro. It is shown that the preparations remove TA antigens from the surface of the tumor cell. It is supposed that the mechanisms of citotoxic effect of extracellular FBFase of B. subtilis 668 and preparation from the culture liquid of B. subtilis B 7025 in vitro on cells of sarcoma 37 is probably realized through the apoptosis.
Assuntos
Antineoplásicos/farmacologia , Apoptose/efeitos dos fármacos , Bacillus subtilis/enzimologia , Meios de Cultura/química , Líquido Extracelular/enzimologia , Frutose-Bifosfatase/farmacologia , Animais , Antineoplásicos/isolamento & purificação , Linhagem Celular Tumoral , Frutose-Bifosfatase/isolamento & purificação , Sarcoma 37/patologiaRESUMO
The p53 tumor-suppressor protein prevents cancer development through various mechanisms, including the induction of cell-cycle arrest, apoptosis, and the maintenance of genome stability. We have identified a p53-inducible gene named TIGAR (TP53-induced glycolysis and apoptosis regulator). TIGAR expression lowered fructose-2,6-bisphosphate levels in cells, resulting in an inhibition of glycolysis and an overall decrease in intracellular reactive oxygen species (ROS) levels. These functions of TIGAR correlated with an ability to protect cells from ROS-associated apoptosis, and consequently, knockdown of endogenous TIGAR expression sensitized cells to p53-induced death. Expression of TIGAR may therefore modulate the apoptotic response to p53, allowing survival in the face of mild or transient stress signals that may be reversed or repaired. The decrease of intracellular ROS levels in response to TIGAR may also play a role in the ability of p53 to protect from the accumulation of genomic damage.
Assuntos
Apoptose/genética , Transformação Celular Neoplásica/metabolismo , Glicólise/genética , Estresse Oxidativo/genética , Monoéster Fosfórico Hidrolases/metabolismo , Proteínas/metabolismo , Proteína Supressora de Tumor p53/metabolismo , Sequência de Aminoácidos , Proteínas Reguladoras de Apoptose , Sequência de Bases , Linhagem Celular Tumoral , Sobrevivência Celular/genética , Transformação Celular Neoplásica/genética , Cromossomos Humanos Par 12/genética , Dano ao DNA/fisiologia , Reparo do DNA/fisiologia , Regulação para Baixo/fisiologia , Metabolismo Energético/genética , Frutose-Bifosfatase/genética , Frutose-Bifosfatase/isolamento & purificação , Frutose-Bifosfatase/metabolismo , Frutosedifosfatos/metabolismo , Regulação da Expressão Gênica/fisiologia , Instabilidade Genômica/fisiologia , Humanos , Peptídeos e Proteínas de Sinalização Intracelular , Dados de Sequência Molecular , Monoéster Fosfórico Hidrolases/genética , Monoéster Fosfórico Hidrolases/isolamento & purificação , Proteínas/genética , Proteínas/isolamento & purificação , Espécies Reativas de Oxigênio/metabolismo , Transdução de Sinais/fisiologia , Proteína Supressora de Tumor p53/genéticaRESUMO
Fructose 1,6-bisphosphatase (FBPase) is known to form a supramolecular complex with alpha-actinin and aldolase on both sides of the Z-line in skeletal muscle cells. It has been proposed that association of aldolase with FBPase not only desensitizes muscle FBPase toward AMP inhibition but it also might enable the channeling of intermediates between the enzymes [Rakus et al. (2003) FEBS Lett. 547, 11-14]. In the present paper, we tested the possibility of fructose 1,6-bisphosphate (F1,6-P(2)) channeling between aldolase and FBPase using the approach in which an inactive form of FBPase competed with active FBPase for binding to aldolase and thus decreased the rate of aldolase-FBPase reaction. The results showed that F1,6-P(2) is transferred directly from aldolase to FBPase without mixing with the bulk phase. Further evidence that F1,6-P(2) is channeled from aldolase to FBPase comes from the experiments investigating the inhibitory effect of a high concentration of magnesium ions on aldolase-FBPase activity. FBPase in a complex with aldolase, contrary to free muscle FBPase, was not inhibited by high Mg(2+) concentrations, which suggests that free F1,6-P(2) was not present in the assay mixture during the reaction. A real-time interaction analysis between aldolase and FBPase revealed a dual role of Mg(2+) in the regulation of the aldolase-FBPase complex stability. A physiological concentration of Mg(2+) increased the affinity of muscle FBPase to muscle aldolase, whereas higher concentrations of the cation decreased the concentration of the complex. We hypothesized that the presence of Mg(2+) stabilizes a positively charged cavity within FBPase and that it might enable an interaction with aldolase. Because magnesium decreased the binding constant (K(a)) between aldolase and FBPase in a manner similar to the decrease of K(a) caused by monovalent cations, it is postulated that electrostatic attraction might be a driving force for the complex formation. It is presumed that the biological relevance of F1,6-P(2) channeling between aldolase and FBPase is protection of this glyconeogenic, as well as glycolytic, intermediate against degradation by cytosolic aldolase, which is one of the most abundant enzyme of glycolysis.
Assuntos
Frutose-Bifosfatase/metabolismo , Frutose-Bifosfato Aldolase/metabolismo , Músculo Esquelético/química , Músculo Esquelético/enzimologia , Especificidade por Substrato , Actinina/metabolismo , Monofosfato de Adenosina/farmacologia , Animais , Cátions Monovalentes/farmacologia , Simulação por Computador , Relação Dose-Resposta a Droga , Inibidores Enzimáticos/farmacologia , Frutose-Bifosfatase/antagonistas & inibidores , Frutose-Bifosfatase/isolamento & purificação , Frutose-Bifosfato Aldolase/antagonistas & inibidores , Frutose-Bifosfato Aldolase/isolamento & purificação , Cinética , Magnésio/metabolismo , Magnésio/farmacologia , Modelos Moleculares , Polietilenoglicóis , Ligação Proteica , Desnaturação Proteica , Fosfato de Piridoxal/análogos & derivados , Fosfato de Piridoxal/farmacologia , Coelhos , Espectrometria de Fluorescência , Eletricidade Estática , Ressonância de Plasmônio de Superfície , Temperatura , o-Ftalaldeído/farmacologiaRESUMO
Fructose-1,6-bisphosphatase (FBPase; EC 3.1.3.11) is strongly inhibited by AMP in vitro and, therefore, at physiological concentrations of substrate and AMP, FBPase should be completely inhibited. Desensitization of rabbit muscle FBPase against AMP inhibition was previously observed in the presence of rabbit muscle aldolase. In this study, we analysed the kinetics of an FBPase catalyzed reaction and interaction between chicken muscle FBPase and chicken muscle aldolase. The initial rate of FBPase reaction vs. substrate concentration shows a maximum activity at a concentration of 20 microM Fru-1,6P2 and then decreases. Assuming rapid equilibrium kinetics, the enzyme-catalyzed reaction was described by the substrate inhibition model, with Ks approximately 5 microM and Ksi approximately 39 microM and factor beta approximately 0.2, describing change in the rate constant (k) of product formation from the ES and ESSi complexes. Based on ultracentrifugation studies, aldolase and FBPase form a hetero-complex with approximately 1:1 stoichiometry with a dissociation constant (Kd) of 3.8 microM. The FBPase-aldolase interaction was confirmed via fluorescence investigation. The aldolase-FBPase interaction results in aldolase fluorescence quenching and its maximum emission spectrum shifting from 344 to 356 nm. The Kd of the FBPase-aldolase complex, determined on the basis of fluorescence changes, is 0.4 microM at 25 degrees C with almost 1:1 stoichiometry. This interaction increases the I(0.5) for the AMP inhibition of FBPase threefold, and slightly affects FBPase affinity to magnesium ions, increasing the Ka and Hill coefficient (n). No effect of aldolase on the FBPase pH optimum was observed. Thus, the decrease in FBPase sensitivity to AMP inhibition enables FBPase to function in vivo thanks to aldolase.
Assuntos
Frutose-Bifosfatase/metabolismo , Frutose-Bifosfato Aldolase/metabolismo , Músculo Esquelético/enzimologia , Monofosfato de Adenosina/metabolismo , Animais , Galinhas , Frutose-Bifosfatase/antagonistas & inibidores , Frutose-Bifosfatase/isolamento & purificação , Frutose-Bifosfato Aldolase/antagonistas & inibidores , Frutose-Bifosfato Aldolase/isolamento & purificação , Cinética , Ligação Proteica , Espectrometria de Fluorescência , Especificidade por SubstratoRESUMO
Real-time interaction analysis, using the BIAcore biosensor, of rabbit muscle FBPase-aldolase complex revealed apparent binding constant [K(Aapp)] values of about 4.4x10(8) M(-1). The stability of the complex was down-regulated by the glycolytic intermediates dihydroxyacetone phosphate and fructose 6-phosphate, and by the regulator of glycolysis and glyconeogenesis--fructose 2,6-bisphosphate. FBPase in a complex with aldolase was entirely insensitive to inhibition by physiological concentrations of AMP (I(0.5) was 1.35 mM) and the cooperativity of the inhibition was not observed. The existence of an FBPase-aldolase complex that is insensitive to AMP inhibition explains the possibility of glycogen synthesis from carbohydrate precursors in vertebrates' myocytes.
Assuntos
Monofosfato de Adenosina/farmacologia , Frutose-Bifosfatase/antagonistas & inibidores , Frutose-Bifosfato Aldolase/antagonistas & inibidores , Músculo Esquelético/enzimologia , Animais , Fosfato de Di-Hidroxiacetona/farmacologia , Inibidores Enzimáticos/farmacologia , Frutose-Bifosfatase/isolamento & purificação , Frutose-Bifosfatase/metabolismo , Frutose-Bifosfato Aldolase/isolamento & purificação , Frutose-Bifosfato Aldolase/metabolismo , Gluconeogênese , Glicólise , Cinética , CoelhosRESUMO
Phosphorylated fructose-1,6-bisphosphatase (FBPase) was isolated from rabbit muscle in an SDS/PAGE homogeneous form. Its dephosphorylation with alkaline phosphatase revealed 2.8 moles of inorganic phosphate per mole of FBPase. The phosphorylated FBPase (P-FBPase) differs from the dephosphorylated enzyme in terms of its kinetic properties like K(m) and k(cat), which are two times higher for the phosphorylated FBPase, and in the affinity for aldolase, which is three times lower for the dephosphorylated enzyme. Dephosphorylated FBPase can be a substrate for protein kinase A and the amount of phosphate incorporated per FBPase monomer can reach 2-3 molecules. Since interaction of muscle aldolase with muscle FBPase results in desensitisation of the latter toward AMP inhibition (Rakus & Dzugaj, 2000, Biochem. Biophys. Res. Commun. 275, 611-616), phosphorylation may be considered as a way of muscle FBPase activity regulation.
Assuntos
Frutose-Bifosfatase/metabolismo , Músculo Esquelético/enzimologia , Animais , Proteínas Quinases Dependentes de AMP Cíclico/metabolismo , Eletroforese em Gel de Poliacrilamida , Frutose-Bifosfatase/química , Frutose-Bifosfatase/isolamento & purificação , Cinética , Modelos Moleculares , Fosfatos/metabolismo , Fosforilação , Conformação Proteica , Coelhos , Especificidade por SubstratoRESUMO
Hypertrehalosemic neuropeptides from the corpora cardiaca such as the decapeptide Bld HrTH bring about a profound switch in the metabolic activity of cockroach fat body during which production of the blood sugar trehalose is stimulated while the catabolism of carbohydrate (glycolysis) is inhibited. The mechanisms of the metabolic switch are not fully understood. Incubation of isolated fat body from the cockroach Blaptica dubia with 10(-8) M Bld HrTH, for 10-60 min, stimulated glycogen breakdown and increased the content of the substrates of both the glycolytic enzyme 6-phosphofructo-1-kinase (PFK, EC 2.7.1.11) and the gluconeogenic enzyme fructose-1,6-bisphosphatase (FBPase, EC 3.1.3.11) in the tissue. The glycolytic signal fructose 2,6-bisphosphate was markedly decreased in fat body on incubation with Bld HrTH. The content of ATP was slightly reduced, while the contents of ADP and AMP were increased after incubation with the hormone. Fructose 2,6-bisphosphate is a potent activator of PFK and a strong inhibitor of FBPase purified from fat body. The activity of PFK was decreased by about 90% when the hormone-dependent changes in effectors and substrates in fat body were simulated in vitro. FBPase, in contrast, was activated about 25-fold under these conditions, suggesting the hormone to stimulate gluconeogenesis in fat body. The data support the view that fructose 2,6-bisphosphate is a pivotal intracellular messenger in the hormone-induced metabolic switch from carbohydrate degradation to trehalose production in cockroach fat body.
Assuntos
Baratas/metabolismo , Corpo Adiposo/metabolismo , Frutose-Bifosfatase/antagonistas & inibidores , Neuropeptídeos/farmacologia , Fosfofrutoquinase-1/antagonistas & inibidores , Monofosfato de Adenosina/farmacologia , Animais , Frutose-Bifosfatase/isolamento & purificação , Frutosedifosfatos/farmacologia , Regulação Enzimológica da Expressão Gênica , Glicogênio/metabolismo , Técnicas In Vitro , Hormônios de Inseto/farmacologia , Cinética , Masculino , Fosfofrutoquinase-1/isolamento & purificação , Trealose/metabolismoRESUMO
F1,6BPases from porcine and bovine lung were isolated and their kinetic properties were determined. Ks, Kis and beta were determined assuming partial-noncompetitive inhibition (simple intersecting hyperbolic noncompetitive inhibition) of the enzyme by the substrate. Values for Ks were 4.1 and 4.4 microM for porcine and bovine F1,6BPase, respectively and values for 1 were close to 0.55 in both cases. Kis were 9 and 15 microM for porcine and bovine F1,6BPase, respectively. I0.5 for AMP were determined as 7 microM for pig enzyme and 14 microM for F1,6BPase from bovine lung. The enzymes were inhibited by F2,6BP with Ki's of 0.19 and 0.21 microM for porcine and bovine enzymes, respectively. In the presence of AMP concentration equal to I0.5, the Ki values for pig and bovine enzymes were 0.07 and 0.09 microM, respectively. The levels of F2,6BP, AMP and antioxidant enzymes activities in pig and bovine lung tissues were also determined. The cDNA coding sequence of pig lung F1,6BPase1 showed a high homology with pig liver enzyme, differing only in four positions (G/C-63, T/A-808, G/C-884 and T/A-1005) resulting in a single amino acid substitution (Gly-295 for Ala-295). It is hypothesized that the lung F1,6BPase participates in gluconeogenesis, surfactant synthesis and antioxidant reactions.
Assuntos
Frutose-Bifosfatase/química , Isoenzimas , Pulmão/enzimologia , Monofosfato de Adenosina/metabolismo , Animais , Catalase/metabolismo , Bovinos , DNA Complementar/metabolismo , Relação Dose-Resposta a Droga , Eletroforese em Gel de Poliacrilamida , Frutose-Bifosfatase/isolamento & purificação , Frutose-Bifosfatase/metabolismo , Frutose-Bifosfatase/fisiologia , Glucose-6-Fosfatase/metabolismo , Glucosefosfato Desidrogenase/metabolismo , Glutationa Redutase/metabolismo , Concentração Inibidora 50 , Cinética , Fígado/enzimologia , Magnésio/farmacologia , Modelos Biológicos , Modelos Químicos , Dados de Sequência Molecular , Oxirredutases/metabolismo , Fosfoenolpiruvato Carboxiquinase (ATP)/metabolismo , Fosfogluconato Desidrogenase/metabolismo , RNA Mensageiro/metabolismo , Especificidade por Substrato , Superóxido Dismutase/metabolismo , SuínosRESUMO
D-Fructose-1,6-bisphosphate 1-phosphohydrolase [EC 3.1.3.11] (Fru-1,6P2ase), a regulatory enzyme of gluconeogenesis, was isolated from Rana esculenta liver in homogeneous from with approximately 30% yield. Basic kinetic properties of the enzyme and its subunit molecular weight were determined. Km is 1.72 microM. Like other vertebrate Fru-1,6P2ase, the frog liver enzyme is inhibited by fructose-2,6-bisphosphate (Fru-2,6P2) competitively, Ki is 78 nM and by AMP allosterically, I0.5 is 10.9 microM. Both inhibitors (Fru-2,6P2 and AMP) act synergistically on liver Fru-1,6-P2ase. Ki for Fru-2,6P2 determined in the presence of 1-10 microM of AMP were 35-2 nM, respectively. Maximum activity was found at pH 7.5. Like other Fru-1,6P2ases, the frog enzyme requires magnesium ions for its activity and is activated by potassium ions; the Ka for Mg2+ is 267 microM, Ka for K+ is 77 mM. The subunit molecular weight of the frog liver Fru-1,6P2ase was 37,300 Da. A great similarity between regulatory properties of frog liver Fru-1,6P2ase and liver enzymes of other vertebrates, suggests a similar regulation of gluconeogenesis in amphibia and other vertebrates.
Assuntos
Frutose-Bifosfatase/isolamento & purificação , Frutose-Bifosfatase/metabolismo , Fígado/enzimologia , Rana esculenta/metabolismo , Vertebrados/metabolismo , Monofosfato de Adenosina/metabolismo , Monofosfato de Adenosina/farmacologia , Animais , Bovinos , Ativação Enzimática/efeitos dos fármacos , Frutose-Bifosfatase/antagonistas & inibidores , Frutosedifosfatos/metabolismo , Humanos , Concentração de Íons de Hidrogênio , Isoenzimas/isolamento & purificação , Isoenzimas/metabolismo , Cinética , Magnésio/metabolismo , Magnésio/farmacologia , Peso Molecular , Fosfofrutoquinase-2 , Monoéster Fosfórico Hidrolases/antagonistas & inibidores , Monoéster Fosfórico Hidrolases/metabolismo , Potássio/metabolismo , Potássio/farmacologia , Coelhos , Ratos , Especificidade da Espécie , SuínosRESUMO
By applying a newly developed method, cDNAs for the human muscle isoform of fructose-1,6-bisphosphatase were isolated from phage- and plasmid-derived libraries. From these cDNAs and an EST clone, a composite sequence (1302 bp) was deduced that contains an open reading frame encoding a polypeptide of 339 amino acids with an estimated molecular weight of 36 755. After overexpression in E. coli, recombinant human muscle fructose 2,6-bisphosphatase was found to be active in cel-free extracts and could be strongly inhibited by AMP and fructose 2,6-bisphosphate. Sequence comparisons revealed that (1) all amino acids thought to be in contact with substrate molecules, regulatory molecules or metal ions in mammalian liver fructose-1,6-bisphosphatases are, with one exception, conserved in the human muscle enzyme and (2) the human muscle isoform is more homologous to the mouse intestine fructose-1,6-bisphosphatase than to the mammalian liver isoform. This is the first report of the cloning and expression of a muscle fructose-1,6-bisphosphatase isoenzyme.
Assuntos
Alelos , DNA Complementar/química , DNA Complementar/isolamento & purificação , Frutose-Bifosfatase/genética , Músculo Esquelético/enzimologia , Sequência de Aminoácidos , Sequência de Bases , Clonagem Molecular , Escherichia coli/enzimologia , Escherichia coli/genética , Frutose-Bifosfatase/biossíntese , Frutose-Bifosfatase/isolamento & purificação , Expressão Gênica , Vetores Genéticos/genética , Humanos , Isoenzimas/biossíntese , Isoenzimas/genética , Isoenzimas/isolamento & purificação , Dados de Sequência Molecular , Fases de Leitura Aberta , Reação em Cadeia da Polimerase , Proteínas Recombinantes/biossíntese , Sequências Reguladoras de Ácido Nucleico , Homologia de Sequência de AminoácidosRESUMO
The primary structure of several chloroplast fructose-1,6-bisphosphatase (CFBPase) was deduced from DNA sequences, but only spinach, pea and rapeseed enzymes have been characterized structurally. We analyzed whether CFBPases from different phylogenetic origin contain a common epitope. To this end a DNA fragment of 1200 base pairs encoding 338 amino acid residues of wheat CFBPase (38 kDa) was cloned in the expression plasmid pGEX-1 in frame with the gene coding for glutathione S-transferase (GT) of Schistosoma japonicun (26.5 kDa). Upon transformation of Escherichia coli and induction with isopropyl-beta-D-thiogalactopyranoside, centrifugation of the lysate partitioned 10% of the fusion protein in the supernatant fraction and the remaining 90% in the precipitate. The expected 65 kDa protein was purified from both the soluble and the particulate fraction by affinity chromatography on columns of glutathione-agarose. This fusion protein was successfully used to produce a monoclonal antibody that specifically recognized the CFBPase region of the fusion protein but not the GT moiety. Moreover, the monoclonal antibody immunoreacted not only with polypeptides (ca. 40 kDa) present in leaf crude extracts of other varieties of wheat (Triticum spelta, T. aestivum and T. durum), but also with homogeneous preparations of the spinach (Spinacia oleracea) and rapeseed (Brassica napus) enzymes. Thus, the cross reaction of this monoclonal antibody with counterparts from different plant species indicates the persistency of a common epitope through biological evolution.
Assuntos
Anticorpos Monoclonais , Cloroplastos/enzimologia , Frutose-Bifosfatase/imunologia , Triticum/enzimologia , Sequência de Aminoácidos , Animais , Especificidade de Anticorpos , Cloroplastos/genética , Cloroplastos/imunologia , Clonagem Molecular , Reações Cruzadas , Epitopos/genética , Epitopos/imunologia , Escherichia coli/genética , Evolução Molecular , Frutose-Bifosfatase/genética , Frutose-Bifosfatase/isolamento & purificação , Glutationa Transferase/genética , Camundongos , Fragmentos de Peptídeos/genética , Fragmentos de Peptídeos/imunologia , Fragmentos de Peptídeos/isolamento & purificação , Plantas/enzimologia , Plantas/genética , Plantas/imunologia , Proteínas Recombinantes de Fusão/genética , Proteínas Recombinantes de Fusão/imunologia , Proteínas Recombinantes de Fusão/isolamento & purificação , Schistosoma japonicum/enzimologia , Schistosoma japonicum/genética , Especificidade da Espécie , Triticum/genética , Triticum/imunologiaRESUMO
The primary structure of rabbit liver fructose-1,6-bisphosphatase was determined by peptide analysis of digests with different proteases. The results establish the primary structure, complete data bank entries, and show that this enzyme variant is indeed homologous with other liver fructose-1,6-bisphosphatases. Residue differences with the enzymes from other mammals are 9-15%, with those from plants and yeasts about 50%, and with those from characterized prokaryotes up to 70%, showing an enzyme variability intermediate between those of 'variable' and 'constant' oligomeric dehydrogenases. Structural relationships, conformations and catalytic mechanisms are consistent within the family of fructose-1,6-bisphosphatases, and the rabbit protein is a typical rather than an aberrant form of the enzyme.
Assuntos
Frutose-Bifosfatase/química , Fígado/enzimologia , Monofosfato de Adenosina/metabolismo , Sequência de Aminoácidos , Animais , Sítios de Ligação , Cromatografia Líquida de Alta Pressão , Endopeptidases/metabolismo , Frutose-Bifosfatase/isolamento & purificação , Frutose-Bifosfatase/metabolismo , Dados de Sequência Molecular , Peptídeos/química , Coelhos , Homologia de Sequência de AminoácidosRESUMO
The structural transformation of fructose-1,6-bisphosphatase upon binding of the allosteric regulator AMP dramatically changes the interactions across the C1-C4 (C2-C3) subunit interface of the enzyme. Asn9, Met18, and Ser87 residues were modified by site-directed mutagenesis to probe the function of the interface residues in porcine liver fructose-1,6-bisphosphatase. The wild-type and mutant forms of the enzyme were purified to homogeneity and characterized by initial rate kinetics and circular dichroism (CD) spectrometry. No discernible alterations in structure were observed among the wild-type and Asn9Asp, Met18Ile, Met18Arg, and Ser87Ala mutant forms of the enzyme as measured by CD spectrometry. Kinetic analyses revealed 1.6- and 1.8-fold increases in kcat with Met18Arg and Asn9Asp, respectively. The K(m) for fructose 1,6-bisphosphate increased about 2-approximately 4-fold relative to that of the wild-type enzyme in the four mutants. A 50-fold lower Ka value for Mg2+ compared with that of the wild-type enzyme was obtained for Met18Ile with no alteration of the Ki for AMP. However, the replacement of Met18 with Arg caused a dramatic decrease in AMP affinity (20 000-fold) without a change in Mg2+ affinity. Increases of 6- and 2-fold in the Ki values for AMP were found with Asn9Asp and Ser87Ala, respectively. There was no difference in the cooperativity for AMP inhibition between the wild-type and the mutant forms of fructose-1,6-bisphosphatase. This study demonstrates that the mutation of residues in the C1-C4 (C2-C3) interface of fructose-1,6-bisphosphatase can significantly affect the affinity for Mg2+, which is presumably bound 30 A away. Moreover the mutations alternatively reduce AMP and Mg2+ affinities, and this finding may be associated with the destabilization of the corresponding allosteric states of the enzyme. The kinetics and structural modeling studies of the interface residues provide new insights into the conformational equilibrium of fructose-1,6-bisphosphatase.
Assuntos
Monofosfato de Adenosina/metabolismo , Frutose-Bifosfatase/química , Frutose-Bifosfatase/metabolismo , Magnésio/metabolismo , Estrutura Secundária de Proteína , Regulação Alostérica , Sequência de Aminoácidos , Sequência de Bases , Primers do DNA , Frutose-Bifosfatase/isolamento & purificação , Cinética , Substâncias Macromoleculares , Matemática , Modelos Estruturais , Dados de Sequência Molecular , Mutagênese Sítio-Dirigida , Mutação Puntual , Proteínas Recombinantes/química , Proteínas Recombinantes/isolamento & purificação , Proteínas Recombinantes/metabolismoRESUMO
Xanthobacter flavus employs two fructosebisphosphatase (FBPase)-sedoheptulosebisphosphatase (SBPase) enzymes. One of these is constitutively expressed and has a high FBPase-to-SBPase ratio. The alternative enzyme, which is encoded by cbbF, is induced during autotrophic growth. The cbbF gene was expressed in Escherichia coli, and the FBPase was purified to homogeneity. The purified enzyme has a specific FBPase activity of 114 mumol/min/mg of protein, a Michaelis constant for fructosebisphosphate of 3 microM, and a low FBPase-to-SBPase ratio. CbbF was activated by ATP and inhibited by Ca2+.