Assuntos
Condrossarcoma/veterinária , Meios de Contraste/administração & dosagem , Doenças do Cão/diagnóstico por imagem , Epistaxe/veterinária , Infecções por Escherichia coli/veterinária , Iohexol/administração & dosagem , Infecções Urinárias/veterinária , Animais , Condrossarcoma/diagnóstico por imagem , Doenças do Cão/urina , Cães , Epistaxe/diagnóstico por imagem , Escherichia coli/isolamento & purificação , Infecções por Escherichia coli/diagnóstico por imagem , Infecções por Escherichia coli/urina , Masculino , Cavidade Nasal/diagnóstico por imagem , Cavidade Nasal/patologia , Concentração Osmolar , Gravidade Específica/efeitos dos fármacos , Tomografia Computadorizada por Raios X/veterinária , Urinálise/veterinária , Infecções Urinárias/diagnóstico por imagem , Infecções Urinárias/urina , Urina/química , Urina/microbiologiaRESUMO
A study was made of apoptotic cell shrinkage, which is generally believed to be a hallmark of apoptosis. The two conventional models of apoptosis were used for examination of changes in cell water balance--one is apoptosis caused in human lymphoma cell line U937 by staurosporine, and the other by etoposide. Intracellular water was determined by measuring buoyant density of cells in continuous Percoll gradient. Apoptosis was recognized by microscopy and flow cytometry. Apoptosis caused by staurosporine (1 microM, 4 h) was found to be associated with a decrease in cell water content by almost 24%. In contrast, no decrease in cell water content was observed in U937 cells incubated with etoposide (50 microM, 4 h), in spite of the number of features suggesting the presence of apoptosis, such as the appearance of apoptotic bodies, chromatin condensation and fragmentation and disappearance of S-phase cells in DNA histogram. It is concluded that definition of apoptosis as "shrinkage-necrosis" (Kerr, 1971) needs correcting: the distinction of apoptotic cells involves the absence of swelling, rather than cell shrinkage.
Assuntos
Antineoplásicos Fitogênicos/farmacologia , Apoptose , Etoposídeo/farmacologia , Estaurosporina/farmacologia , Tamanho Celular/efeitos dos fármacos , Citometria de Fluxo , Humanos , Microscopia de Fluorescência , Gravidade Específica/efeitos dos fármacos , Células U937 , Água/análiseRESUMO
Nephrogenic diabetes insipidus was diagnosed in a dog with an intestinal leiomyosarcoma. The diagnosis of nephrogenic diabetes insipidus was made on the basis of results of serum biochemical tests, urinalyses, and a water-deprivation test, along with a lack of response to exogenous administration of vasopressin following the water-deprivation test. The temporal association between resection of the intestinal mass and resolution of clinical signs of diabetes insipidus (i.e., polyuria and polydipsia) and between recurrence of clinical signs and detection of metastatic disease suggests that there may have been a causal relationship, and nephrogenic diabetes insipidus may have developed as a paraneoplastic syndrome in this dog.