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1.
PLoS Negl Trop Dis ; 12(8): e0005840, 2018 08.
Artigo em Inglês | MEDLINE | ID: mdl-30138310

RESUMO

We briefly review cysteine proteases (orthologs of mammalian cathepsins B, L, F, and C) that are expressed in flatworm and nematode parasites. Emphasis is placed on enzyme activities that have been functionally characterized, are associated with the parasite gut, and putatively contribute to degrading host proteins to absorbable nutrients [1-4]. Often, gut proteases are expressed as multigene families, as is the case with Fasciola [5] and Haemonchus [6], presumably expanding the range of substrates that can be degraded, not least during parasite migration through host tissues [5]. The application of the free-living planarian and Caenorhabditis elegans as investigative models for parasite cysteine proteases is discussed. Finally, because of their central nutritive contribution, targeting the component gut proteases with small-molecule chemical inhibitors and understanding their utility as vaccine candidates are active areas of research [7].


Assuntos
Cisteína Proteases/metabolismo , Digestão/fisiologia , Helmintos/enzimologia , Parasitos/enzimologia , Animais , Helmintos/metabolismo , Parasitos/metabolismo
2.
PLoS Negl Trop Dis ; 12(8): e0005919, 2018 08.
Artigo em Inglês | MEDLINE | ID: mdl-30138448

RESUMO

Neglected tropical diseases caused by metazoan parasites are major public health concerns, and therefore, new methods for their control and elimination are needed. Research over the last 25 years has revealed the vital contribution of cysteine proteases to invasion of and migration by (larval) helminth parasites through host tissues, in addition to their roles in embryogenesis, molting, egg hatching, and yolk degradation. Their central function to maintaining parasite survival in the host has made them prime intervention targets for novel drugs and vaccines. This review focuses on those helminth cysteine proteases that have been functionally characterized during the varied early stages of development in the human host and embryogenesis.


Assuntos
Cisteína Proteases/metabolismo , Helmintos/efeitos dos fármacos , Helmintos/enzimologia , Animais , Inibidores de Cisteína Proteinase , Humanos , Doenças Negligenciadas/tratamento farmacológico , Doenças Negligenciadas/parasitologia , Parasitos/efeitos dos fármacos , Parasitos/enzimologia
3.
Curr Med Chem ; 20(25): 3078-102, 2013.
Artigo em Inglês | MEDLINE | ID: mdl-23514418

RESUMO

Protease function is essential to many biological systems and processes. In parasites, proteases are essential for host tissue degradation, immune evasion, and nutrition acquisition. Helminths (worms) depend on several classes of proteases for development, host tissue invasion and migration, and for degradation of host hemoglobin and serum proteins. The protozoa, which cause malaria, depend on both cysteine and aspartic proteases to initiate host hemoglobin digestion. Other types of proteases are involved in erythrocyte cell invasion and cell exit. Surface metalloproteases in kinetoplastids are implicated in the evasion of complement-mediated cell lysis and cell entry. Cysteine proteases in Entamoeba facilitate invasion of the host colon. Giardia utilizes a cysteine protease for both encystation and excystation. This review will summarize published data using protease inhibitors as tools to identify the function of parasite proteases in the development, virulence, and pathogenesis of parasites; as well as the role of endogenous parasite protease inhibitors in regulation.


Assuntos
Proteínas de Helminto/antagonistas & inibidores , Helmintos/patogenicidade , Inibidores de Proteases/química , Aminopeptidases/antagonistas & inibidores , Aminopeptidases/metabolismo , Animais , Cestoides/enzimologia , Cestoides/crescimento & desenvolvimento , Cestoides/patogenicidade , Cistatinas/farmacologia , Cisteína Proteases/química , Cisteína Proteases/metabolismo , Proteínas de Helminto/metabolismo , Helmintos/efeitos dos fármacos , Helmintos/enzimologia , Humanos , Nematoides/enzimologia , Nematoides/crescimento & desenvolvimento , Nematoides/patogenicidade , Inibidores de Proteases/farmacologia , Serpinas/farmacologia , Trematódeos/enzimologia , Trematódeos/crescimento & desenvolvimento , Trematódeos/patogenicidade , Virulência/efeitos dos fármacos
4.
Artigo em Chinês | MEDLINE | ID: mdl-24818401

RESUMO

Cathepsin F is an important member of papain-like subfamily in cysteine protease family. Cathepsin F of helminth parasites can hydrolyze the specific substrate, degrade host protein such as hemoglobin for nutrition, and be involved in invasion into host tissue. Therefore, cathepsin F serves as a potential target for parasitic disease immunodiagnosis, vaccine design and anti-parasite drug screening. This article reviews the structural characteristics and mechanisms of cathepsin F, and research advances on cathepsin F of parasitic helminths.


Assuntos
Catepsina F , Helmintos/enzimologia , Animais
6.
J Allergy Clin Immunol ; 130(1): 248-56.e9, 2012 Jul.
Artigo em Inglês | MEDLINE | ID: mdl-22541242

RESUMO

BACKGROUND: The extensive similarities between helminth proteins and allergens are thought to contribute to helminth-driven allergic sensitization. OBJECTIVE: The objective of this study was to investigate the cross-reactivity between a major glutathione-S transferase allergen of cockroach (Bla g 5) and the glutathione-S transferase of Wuchereria bancrofti (WbGST), a major lymphatic filarial pathogen of humans. METHODS: We compared the molecular and structural similarities between Bla g 5 and WbGST by in silico analysis and by linear epitope mapping. The levels of IgE, IgG, and IgG(4) antibodies were measured in filarial-infected and filarial-uninfected patients. Mice were infected with Heligmosomoides bakeri, and their skin was tested for cross-reactive allergic responses. RESULTS: These 2 proteins are 30% identical at the amino acid level with remarkable similarity in the N-terminal region and overall structural conservation based on predicted 3-dimensional models. Filarial infection was associated with IgE, IgG, and IgG(4) anti-Bla g 5 antibody production, with a significant correlation between antibodies (irrespective of isotype) to Bla g 5 and WbGST (P< .0003). Preincubation of sera from cockroach-allergic subjects with WbGST partially depleted (by 50%-70%) anti-Bla g 5 IgE, IgG, and IgG(4) antibodies. IgE epitope mapping of Bla g 5 revealed that 2 linear N-terminal epitopes are highly conserved in WbGST corresponding to Bla g 5 peptides partially involved in the inhibition of WbGST binding. Finally, mice infected with H bakeri developed anti-HbGST IgE and showed immediate-type skin test reactivity to Bla g 5. CONCLUSION: These data demonstrate that helminth glutathione-S transferase and the aeroallergen Bla g 5 share epitopes that can induce allergic cross-sensitization.


Assuntos
Anticorpos/sangue , Baratas/enzimologia , Glutationa Transferase/genética , Glutationa Transferase/imunologia , Helmintos/enzimologia , Mimetismo Molecular/imunologia , Sequência de Aminoácidos , Animais , Baratas/genética , Baratas/imunologia , Reações Cruzadas , Filariose Linfática/imunologia , Mapeamento de Epitopos , Feminino , Glutationa Transferase/química , Proteínas de Helminto/química , Proteínas de Helminto/genética , Proteínas de Helminto/imunologia , Helmintos/imunologia , Humanos , Imunoglobulina E/sangue , Imunoglobulina E/imunologia , Proteínas de Insetos/química , Proteínas de Insetos/genética , Proteínas de Insetos/imunologia , Camundongos , Camundongos Endogâmicos BALB C , Modelos Moleculares , Dados de Sequência Molecular , Filogenia , Análise de Sequência de DNA , Trichostrongyloidea/imunologia , Tricostrongiloidíase/imunologia , Wuchereria bancrofti/enzimologia , Wuchereria bancrofti/genética , Wuchereria bancrofti/imunologia
7.
Trends Parasitol ; 25(12): 573-81, 2009 Dec.
Artigo em Inglês | MEDLINE | ID: mdl-19854678

RESUMO

The accumulation of sequenced genomes has expanded the already sizeable population of cysteine peptidases from parasites. Characterization of a few of these enzymes has ascribed key roles to peptidases in parasite life cycles and has also shed light on mechanisms of pathogenesis. Here we discuss recent observations on the physiological activities of cysteine peptidases of parasitic organisms, paired with a global view of all cysteine peptidases from the MEROPS database grouped by similarity. This snapshot of the landscape of parasite cysteine peptidases is complex and highly populated, suggesting that expansion of research beyond the few 'model' parasite peptidases is now timely.


Assuntos
Cisteína Proteases/metabolismo , Interações Hospedeiro-Patógeno/fisiologia , Parasitos/enzimologia , Animais , Apicomplexa/enzimologia , Cisteína Proteases/genética , Euglenozoários/enzimologia , Variação Genética , Genoma Helmíntico , Genoma de Protozoário , Helmintos/enzimologia , Humanos , Parasitos/genética
9.
Trends Biochem Sci ; 33(12): 601-8, 2008 Dec.
Artigo em Inglês | MEDLINE | ID: mdl-18848453

RESUMO

Helminth pathogens express papain-like cysteine peptidases, termed cathepsins, which have important roles in virulence, including host entry, tissue migration and the suppression of host immune responses. The liver fluke Fasciola hepatica, an emerging human pathogen, expresses the largest cathepsin L cysteine protease family yet described. Recent phylogenetic, biochemical and structural studies indicate that this family contains five separate clades, which exhibit overlapping but distinct substrate specificities created by a process of gene duplication followed by subtle residue divergence within the protease active site. The developmentally regulated expression of these proteases correlates with the passage of the parasite through host tissues and its encounters with different host macromolecules.


Assuntos
Catepsinas/fisiologia , Cisteína Endopeptidases/fisiologia , Fasciola hepatica/enzimologia , Sequência de Aminoácidos , Animais , Catepsinas/genética , Cisteína Endopeptidases/genética , Regulação da Expressão Gênica no Desenvolvimento , Proteínas de Helminto/genética , Proteínas de Helminto/fisiologia , Helmintos/enzimologia , Interações Hospedeiro-Parasita/fisiologia , Humanos , Dados de Sequência Molecular , Filogenia , Alinhamento de Sequência , Especificidade por Substrato
10.
Chem Immunol Allergy ; 90: 45-64, 2006.
Artigo em Inglês | MEDLINE | ID: mdl-16210902

RESUMO

Proteolytic activity is a central biochemical property that endows molecules with intrinsic allergenicity. Thus, the cysteine protease of dust mite, Der p1, the aspartic protease of cockroach, Bla g 2, the serine protease of Aspergillus fumigatus and the bacterial subtilisins are all major allergenic molecules responsible for the increase in asthma and atopic conditions worldwide. These proteases induce Th2-driven inflammatory responses in the airways by disrupting the epithelial cell junctions so that these, and other molecules, gain access to, and alter the function of, underlying cells of the innate immune system (dendritic cells, mast cells, basophils and macrophages) and B and T cells. Helminth parasites secrete proteases to gain entry into their hosts, and to feed on and migrate through tissues. Their action leads to tissue damage and the activation of inflammatory responses dominated by elevated IgE, eosinophilia and Th2 cells, much like allergenic responses. In certain situations, such as in acute infections (especially with zoonotic helminths), proteases secreted by helminths may sensitise individuals to allergens. However, the anti-inflammatory responses observed in chronic helminthiases, involving IL-10 and TGFBeta, that are primarily responsible for controlling immune-mediated damage to the host that is initiated by secreted proteases, coincidentally protects against similar inflammatory damage by allergens.


Assuntos
Alérgenos/imunologia , Endopeptidases/imunologia , Endopeptidases/metabolismo , Helmintíase/imunologia , Helmintíase/parasitologia , Helmintos/enzimologia , Helmintos/imunologia , Animais , Endopeptidases/química , Humanos , Imunoglobulina E/imunologia , Inflamação/enzimologia , Inflamação/imunologia , Inflamação/parasitologia
12.
J Parasitol ; 89(4): 709-14, 2003 Aug.
Artigo em Inglês | MEDLINE | ID: mdl-14533679

RESUMO

This article focuses on the initiation pathway of mucin-type O-glycosylation in helminth parasites. The presence of the GalNAc-O-Ser/Thr structure, also known as Tn antigen, a truncated determinant related to aberrant glycosylation in mammal cells, and the activity of the UDP-GalNAc:polypeptide N-acetyl-galactosaminyltransferase (ppGaNTase), the enzyme responsible for its synthesis, were studied in species from major taxonomic groups. Tn reactivity was determined in extracts from Taenia hydatigena, Mesocestoides corti, Fasciola hepatica, Nippostrongylus brasiliensis, and Toxocara canis using the monoclonal antibody 83D4. The Tn determinant was revealed in all preparations, and multiple patterns of Tn-bearing glycoproteins were observed by immunoblotting. Additionally, the first evidence that helminth parasites express ppGaNTase activity was obtained. This enzyme was studied in extracts from Echinococcus granulosus, F. hepatica, and T. canis by measuring the incorporation of UDP-(3H)GalNAc to both deglycosylated ovine syalomucin (dOSM) and synthetic peptide sequences derived from tandem repeats of human mucins. Whereas significant levels of ppGaNTase activity were detected in all the extracts when dOSM was used as a multisite acceptor, it was only observed in F. hepatica and E. granulosus extracts when mucin-derived peptides were used, suggesting that T. canis ppGaNTase enzyme(s) may represent a member of the gene family with a more restricted specificity for worm O-glycosylation motifs. The widespread expression of Tn antigen, capable of evoking both humoral and cellular immunity, strongly suggests that simple mucin-type O-glycosylation does not constitute an aberrant phenomenon in helminth parasites.


Assuntos
Antígenos Glicosídicos Associados a Tumores/metabolismo , Helmintos/metabolismo , N-Acetilgalactosaminiltransferases/metabolismo , Animais , Antígenos Glicosídicos Associados a Tumores/química , Western Blotting , Bovinos , Cães , Echinococcus/enzimologia , Echinococcus/imunologia , Echinococcus/metabolismo , Eletroforese em Gel de Poliacrilamida , Fasciola hepatica/enzimologia , Fasciola hepatica/imunologia , Fasciola hepatica/metabolismo , Glicopeptídeos/metabolismo , Glicoproteínas/análise , Glicosilação , Helmintos/enzimologia , Helmintos/imunologia , Humanos , Mesocestoides/enzimologia , Mesocestoides/imunologia , Mesocestoides/metabolismo , Camundongos , Nippostrongylus/enzimologia , Nippostrongylus/imunologia , Nippostrongylus/metabolismo , Ratos , Ratos Wistar , Taenia/enzimologia , Taenia/imunologia , Taenia/metabolismo , Toxocara canis/enzimologia , Toxocara canis/imunologia , Toxocara canis/metabolismo , Polipeptídeo N-Acetilgalactosaminiltransferase
13.
J Leukoc Biol ; 73(1): 165-71, 2003 Jan.
Artigo em Inglês | MEDLINE | ID: mdl-12525574

RESUMO

The proteolytic activities frequently associated with sources of allergens and parasite secretions have been suggested as important immunomodulators. We have investigated whether the protease activity of the house dust mite allergen Der p1 and the secreted proteases of the hookworm Necator americanus are able to directly induce type 2 cytokine production by basophils. Der p1 and the secretions of N. americanus induced interleukin (IL)-4, IL-5, and IL-13 but not interferon-gamma mRNA in KU812 basophils. Enzyme-linked immunosorbent assay confirmed that IL-4 and IL-13 were secreted. A nonproteolytic antigen failed to induce cytokine expression, and preincubation of Der p1 or N. americanus secretions with protease inhibitors inhibited cytokine expression. Data were confirmed using basophils purified from human peripheral blood. We speculate that this innate mechanism may contribute to the development of a cytokine milieu that could promote immunoglobulin E synthesis, eosinophil recruitment, and the development of type 2 T cells.


Assuntos
Basófilos/imunologia , Citocinas/biossíntese , Endopeptidases/imunologia , Helmintos/enzimologia , Pyroglyphidae/enzimologia , Animais , Antígenos de Dermatophagoides/imunologia , Antígenos de Dermatophagoides/farmacologia , Proteínas de Artrópodes , Basófilos/metabolismo , Cisteína Endopeptidases , Citocinas/efeitos dos fármacos , Endopeptidases/farmacologia , Regulação da Expressão Gênica/efeitos dos fármacos , Regulação da Expressão Gênica/imunologia , Helmintos/imunologia , Humanos , Interferon gama , Interleucina-13/biossíntese , Interleucina-13/metabolismo , Interleucina-4/biossíntese , Interleucina-4/metabolismo , Interleucina-5/biossíntese , Necator americanus/enzimologia , Necator americanus/imunologia , Pyroglyphidae/imunologia , Células Th2/imunologia
14.
Exp Parasitol ; 101(2-3): 83-9, 2002.
Artigo em Inglês | MEDLINE | ID: mdl-12427461

RESUMO

This study compared specific protein sequence motifs present within cathepsin B-like cysteine proteases from a number of helminth parasites. We have focused our efforts on cathepsin B-like proteases of Haemonchus contortus, Caenorhabditis elegans, Schistosoma mansoni, Schistosoma japonicum, Ostertagia ostertagi, and Ancylostoma caninum. The goal of this work is to correlate specific features, or proposed roles, of the cathepsin B-like proteases with primary sequence motifs discovered within the proteins. We report here a general motif for the identification of cathepsin B enzymes, and more significantly, a motif within this pattern that is found, with one exception, only in cathepsin B-like proteases of helminth bloodfeeders. We suggest that the "hemoglobinase" motif arose evolutionarily in a minimum of three independent events as a specialized response to increase the efficiency of hemoglobin degradation by these cathepsin B-like enzymes. This motif should be useful in identifying additional helminth hemoglobinases and may provide a specific target for drug design efforts.


Assuntos
Catepsina B/química , Cisteína Endopeptidases/química , Proteínas de Helminto , Helmintos/enzimologia , Ancylostoma/enzimologia , Animais , Caenorhabditis elegans/enzimologia , Haemonchus/enzimologia , Helmintos/classificação , Ostertagia/enzimologia , Filogenia , Schistosoma japonicum/enzimologia , Schistosoma mansoni/enzimologia
15.
Adv Parasitol ; 43: 161-266, 1999.
Artigo em Inglês | MEDLINE | ID: mdl-10214692

RESUMO

Many parasites have deployed proteinases to accomplish some of the tasks imposed by a parasitic life style, including tissue penetration, digestion of host tissue for nutrition and evasion of host immune responses. Information on proteinases from trematodes, cestodes and nematode parasites is reviewed, concentrating on those worms of major medical and economical importance. Their biochemical characterization is discussed, along with their putative biological roles and, where available, their associated genes. For example, proteinases expressed by the various stages of the schistosome life-cycle, in particular the well-characterized cercarial elastase which is involved in the penetration of the host skin and the variety of proteinases, such as cathepsin B (Sm31), cathepsin L1, cathepsin L2, cathepsin D, cathepsin C and legumain (Sm32), which are believed to be involved in the catabolism of host haemoglobin. The various endo- and exoproteinases of Fasciola hepatica, the causative agent of liver fluke disease, are reviewed, and recent reports of how these enzymes have been successfully employed in cocktail vaccines are discussed. The various proteinases of cestodes and of the diverse superfamilies of parasitic nematodes are detailed, with special attention being given to those parasites for which most is known, including species of Taenia, Echinococcus, Spirometra, Necator, Acylostoma and Haemonchus. By far the largest number of papers in the literature and entries to the sequence data bases dealing with proteinases of parasitic helminths report on enzymes belonging to the papain superfamily of cysteine proteinases. Accordingly, the final section of the review is devoted to a phylogenetic analysis of this superfamily using over 150 published sequences. This analysis shows that the papain superfamily can be divided into two major branches. Branch A contains the cathepin Bs, the cathepsin Cs and a novel family termed cathepsin Xs, while Branch B contains the cruzipains, cathepsin Ls, papain-like and aleurain/cathepsin H-like proteinases. The relationships of the helminth proteinases, and similar proteinases from protozoan parasites and other organisms, within these groups are discussed.


Assuntos
Endopeptidases/genética , Endopeptidases/metabolismo , Genes de Helmintos , Helmintíase/parasitologia , Helmintos/genética , Sequência de Aminoácidos , Animais , Endopeptidases/química , Endopeptidases/classificação , Helmintos/enzimologia , Humanos , Dados de Sequência Molecular , Filogenia
16.
Int J Parasitol ; 27(8): 883-97, 1997 Aug.
Artigo em Inglês | MEDLINE | ID: mdl-9292304

RESUMO

This paper reviews current knowledge regarding the metabolism of the sulphur-containing amino acids methionine and cysteine in parasitic protozoa and helminths. Particular emphasis is placed on the unusual aspects of parasite biochemistry which may present targets for rational design of antiparasite drugs. In general, the basic pathways of sulphur amino acid metabolism in most parasites resemble those of their mammalian hosts, since the enzymes involved in (a) the methionine cycle and S-adenosylmethionine metabolism, (b) the trans-sulphuration sequence, (c) the transminative catabolism of methionine, (d) the oxidative catabolism of cysteine and (e) glutathione synthesis have been demonstrated variously in several helminth and protozoan species. Despite these common pathways, there also exist numerous differences between parasite and mammalian metabolism. Some of these differences are relatively subtle. For example, the biochemical properties (and primary amino acid structures) of certain parasite methionine cycle enzymes and S-adenosylmethionine decarboxylases differ from those of the corresponding mammalian enzymes, and nematodes and trichomonads possess a novel, non-mammalian form of the trans-sulphuration enzyme cystathionine beta-synthase. The most profound differences between parasite and mammalian biochemistry relate to a number of unusual enzymes and thiol metabolites found in parasitic protozoa. In certain protozoa the pathway for methionine recycling from 5'-methylthioadenosine differs markedly from the mammalian route, and involves 2 exclusively microbial enzymes. Trypanosomatid protozoa contain the non-mammalian antioxidant thiol compounds ovothiol A and trypanothione, together with unique trypanothione-linked enzymes. Specific anaerobic protozoa possess another exclusively microbial enzyme, methionine gamma-lyase, which catabolises methionine (and homocysteine); the physiological significance of these non-mammalian activities is not fully understood. These unusual features offer opportunities for chemotherapeutic exploitation, and in some cases represent metabolic similarities with bacteria. Additionally, some anaerobic protozoa contain unidentified thiols and this implies the presence of further unusual enzymes/pathways in these organisms. So far, no truly unique targets for chemotherapy have been found in helminth sulphur amino acid metabolism, and to some degree this reflects the relative lack of detailed study in the area.


Assuntos
Aminoácidos Sulfúricos/metabolismo , Eucariotos/metabolismo , Helmintos/metabolismo , Animais , Cistationina/metabolismo , Cisteína/metabolismo , Desoxiadenosinas/metabolismo , Eucariotos/enzimologia , Helmintíase/tratamento farmacológico , Helmintos/enzimologia , Humanos , Metionina/metabolismo , Infecções por Protozoários/tratamento farmacológico , S-Adenosilmetionina/metabolismo , Tionucleosídeos/metabolismo
18.
Immunol Cell Biol ; 71 ( Pt 2): 141-3, 1993 Apr.
Artigo em Inglês | MEDLINE | ID: mdl-8486397

RESUMO

A hypothesis is proposed asking why enzyme neutralization is not an effective host-response to a parasite despite the fact that some parasite housekeeping enzymes are highly immunogenic. It is hypothesized that although the structural domain can be immunogenic, the active sites of the parasite enzyme molecules have converged evolutionarily to resemble the functional part (active sites) of host's enzyme molecules, by structural modification/rearrangement (amino acid substitution/polypeptide chain folding) with the effect: (i) of functional adaptation to the host environment; and (ii) to escape detection of active sites by the host as non-self, allowing the parasite to be exposed to antiparasite enzyme antibodies, without deleterious effects on the parasite.


Assuntos
Interações Hospedeiro-Parasita/imunologia , Animais , Enzimas/genética , Enzimas/imunologia , Helmintos/enzimologia , Helmintos/genética , Helmintos/imunologia , Interações Hospedeiro-Parasita/genética , Modelos Biológicos , Seleção Genética
19.
Exp Parasitol ; 72(1): 15-23, 1991 Jan.
Artigo em Inglês | MEDLINE | ID: mdl-1993461

RESUMO

Polyamine levels of some helminth parasites were analyzed by reverse phase HPLC of benzoyl derivatives. Setaria cervi, Acanthocheilonema viteae, Hymenolepis nana, H. diminuta, and Ascaridia galli contained higher levels of spermine than spermidine while in Ancylostoma ceylanicum and Nippostrongylus brasiliensis the spermidine levels were higher than spermine; putrescine was either absent or present in minor quantities. The enzymes of polyamine biosynthesis viz., ornithine decarboxylase, S-adenosyl methionine (SAM)-decarboxylase, and arginine decarboxylase were present in very low to negligible amounts in all the parasites examined. A. ceylanicum exhibited high activity of ornithine amino transferase (OAT) and catalyzed appreciable decarboxylation of ornithine. The ornithine decarboxylating activity of A. ceylanicum was localized in the particulate fraction containing mitochondria, not inhibited by alpha-difluoromethyl ornithine, the specific inhibitor of ornithine decarboxylase (ODC), but inhibited in the presence of glutamate, suggesting the involvement of mitochondrial OAT rather than a true ODC in ornithine decarboxylation in this parasite. Significant activity of polyamine oxidase was also detected in helminth parasites. The absence of polyamine biosynthesizing enzymes in helminth parasites suggests their dependence on hosts for uptake and interconversion of polyamines, providing a potential target for chemotherapy.


Assuntos
Helmintos/metabolismo , Poliaminas/metabolismo , Ancylostoma/enzimologia , Ancylostoma/metabolismo , Animais , Ascaridia/enzimologia , Ascaridia/metabolismo , Cromatografia Líquida de Alta Pressão , Dipetalonema/enzimologia , Dipetalonema/metabolismo , Feminino , Filarioidea/enzimologia , Filarioidea/metabolismo , Helmintos/enzimologia , Hymenolepis/enzimologia , Hymenolepis/metabolismo , Masculino , Nippostrongylus/enzimologia , Nippostrongylus/metabolismo , Ornitina Descarboxilase/análise , Oxirredutases atuantes sobre Doadores de Grupo CH-NH/análise , Setaríase/parasitologia , Poliamina Oxidase
20.
Mol Biochem Parasitol ; 42(2): 205-11, 1990.
Artigo em Inglês | MEDLINE | ID: mdl-2270103

RESUMO

Crude extracts from a number of helminths including Schistosoma intercalatum and Fasciola hepatica were able to detoxify known aldehydic products of lipid peroxidation. A major route for alk-2-enal and alka-2,4-dienal detoxification in parasitic helminths was via glutathione conjugation and glutathione transferase appeared to be responsible for the activity. As yet uncharacterised NADPH-linked systems may provide an important secondary pathway for detoxification of alk-2-enals and alka-2,4-dienals in parasitic helminths. The free-living nematode Panagrellus redivivus had higher active NADH/NADPH-linked aldehyde reduction systems compared to parasitic helminths. The NADH linked and NADPH linked reductions in P. redivivus were mitochondrial and cytosolic activities respectively. NADH/NADPH-linked systems may be responsible for alkanal reduction in helminths as there is no evidence of conjugation of alkanals with glutathione. P. redivivus and Haemonchus contortus were also able to oxidise aldehydes via NAD/NADP-linked systems.


Assuntos
Helmintos/enzimologia , Aldeído Desidrogenase/metabolismo , Animais , Glutationa/farmacologia , Glutationa Peroxidase/metabolismo , Glutationa Transferase/metabolismo , Helmintos/efeitos dos fármacos , Peroxidação de Lipídeos , NAD/farmacologia , NADP/farmacologia
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