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1.
Int J Radiat Biol ; 99(9): 1433-1438, 2023.
Artigo em Inglês | MEDLINE | ID: mdl-36881510

RESUMO

PURPOSE: This study aimed to assess specific gamma irradiation doses to be applied as a post-preparation process to assure the chemical, physical and sensory properties of plum molasses (PM). MATERIALS AND METHODS: Samples of PM were treated with 0, 3, 6 and 9 kGy in a 60Co gamma irradiation plant. Proximate composition, chemical, the physical and sensory determination was accomplished immediately after treatment. RESULTS: Our results showed that, the moisture content of PM were significantly (p < .05) rise by treating with 3 kGy. Whereas, ash and reducing sugar quantity of PM was significantly (p < .05) decreased by treatment at the same dose (3 kGy). Irradiation treatment induced small and not significant alterations (p > .05) in crude protein, crud fat, and total sugar of PM. The chemical and physical parameters including; total acidity (TA), pH, volatile basic nitrogen (VBN), total soluble solids (TSS) (%, Brix), viscosity and color, which were elected as the indicators of quality, were all well within the recommended values for PM treated with 0, 3, 6 and 9 kGy. Sensory determination indicated no significant (p > .05) alterations between irradiated samples and non-irradiated samples of PM. CONCLUSION: Irradiation treatment at 3 kGy was considered as acceptable to be used for the preservation purpose of PM without modifying their quality properties.


Assuntos
Irradiação de Alimentos , Prunus domestica , Raios gama , Melaço , Açúcares , Irradiação de Alimentos/métodos
2.
Meat Sci ; 184: 108700, 2022 Feb.
Artigo em Inglês | MEDLINE | ID: mdl-34768181

RESUMO

The current investigation assessed the effect of pectin (PE) biodegradable nanocomposite coating containing curcumin nanoparticles (CNP) and ajowan (Carum copticum) essential oil nanoemulsion (ANE) combined with low-dose gamma irradiation on microbial, physiochemical, and sensorial qualities of lamb loins during refrigeration conditions. Active coating combined with gamma irradiation reduced the count number of mesophilic and psychrotrophic bacteria, lactic acid bacteria, Enterobacteriaceae; and minimized lipid and protein oxidation changes, total volatile basic nitrogen content, met-myoglobin formation, and color deterioration in the loin samples. The increased shelf-life of lamb loins up to 25 days compared with 5 days assigned for the control group can be associated with the application of ionizing radiation and edible PE coating containing CNP and ANE, which might be due to the synergistic or additive effects of treatments. Overall, as an effective preservation technique, a combination of PE + CNP + ANE and irradiation can be recommended for prolonging the shelf-life of lamb loins during refrigerated storage.


Assuntos
Conservação de Alimentos/métodos , Raios gama , Óleos Voláteis , Carne Vermelha/análise , Carne Vermelha/microbiologia , Animais , Carum/química , Curcumina/química , Irradiação de Alimentos/métodos , Microbiologia de Alimentos , Armazenamento de Alimentos/métodos , Nanocompostos , Pectinas/química , Ovinos
3.
J Appl Microbiol ; 129(5): 1227-1237, 2020 Nov.
Artigo em Inglês | MEDLINE | ID: mdl-32418285

RESUMO

AIMS: The objective of this study was to evaluate the antimicrobial effects of radio frequency (RF) heating and the combination treatment of RF heating with ultraviolet (UV) radiation against foodborne pathogens in roasted grain powder (RGP). METHODS AND RESULTS: Foodborne pathogens inoculated on RGP were subjected to RF heating or RF-UV combination treatments. After 120 s of RF heating, 4·68, 3·89 and 4·54 log reductions were observed for Escherichia coli, Salmonella Typhimurium and Bacillus cereus vegetative cells respectively. The combined RF-UV treatment showed synergistic effects of over 1 log unit compared to the sum of individual treatment for E. coli and S. Typhimurium, but not for B. cereus vegetative cells because of their high UV resistance. Germinated B. cereus cells were not significantly inactivated by RF heating (<1 log CFU per gram), and increased heat resistance compared to the vegetative cells was verified with mild heat treatment. The colour of RGP was not significantly affected by the RF or RF-UV treatments. CONCLUSIONS: Applying RF heating to grain-based food products has advantages for the inactivation of E. coli and S. Typhimurium in RGP. SIGNIFICANCE AND IMPACT OF THE STUDY: The results of the present study could be used as a basis for determining the treatment conditions for inactivating E. coli and other foodborne pathogens such as S. Typhimurium and B. cereus in RGP.


Assuntos
Bactérias/efeitos da radiação , Grão Comestível/microbiologia , Irradiação de Alimentos/métodos , Bactérias/crescimento & desenvolvimento , Contagem de Colônia Microbiana , Microbiologia de Alimentos , Doenças Transmitidas por Alimentos/microbiologia , Viabilidade Microbiana/efeitos da radiação , Ondas de Rádio , Esporos Bacterianos/fisiologia , Esporos Bacterianos/efeitos da radiação , Raios Ultravioleta
4.
J Appl Microbiol ; 128(6): 1534-1546, 2020 Jun.
Artigo em Inglês | MEDLINE | ID: mdl-31991509

RESUMO

AIMS: The objective was to evaluate the possible synergistic effect of cranberry juice (CJ) and commercial citrus extract (BS) against FCV-F9 viral titre in vitro in combination with γ-irradiation and to determinate the D10 values and radiosensitivity increase. METHODS AND RESULTS: Virus samples were treated with a formulation containing a mixture of BS or CJ. Results showed a D10 of 0·05, 0·42% and 1·34 kGy for the virus treated with the BS, the CJ and the irradiation alone respectively. Concentrations needed to reduce 6 log TCID50  ml-1 of viral titre were BS-0·3%, CJ-2·52% and 8·04 kGy. Irradiation combined with BS-0·01% and CJ-0·1% against FCV-F9 virus showed D10 values of 0·74 and 0·72 kGy, respectively, resulting in a viral radiosensitization of 1·28 and 1·50 for respective treatments. CONCLUSION: The higher viral radiosensitization observed after combining γ-irradiation with BS-0·01% and CJ-0·1% indicates that CJ and BS could be used as antiviral agents alone or in combination with γ-irradiation to prevent NoV outbreaks. SIGNIFICANCE AND IMPACT OF THE STUDY: Cranberry juice and BS could be used in hurdle approaches in combined treatment with γ-irradiation to assure food safety without a detrimental effect on nutritional value and maintain low processing cost.


Assuntos
Antivirais/farmacologia , Calicivirus Felino/fisiologia , Irradiação de Alimentos/métodos , Raios gama , Tolerância a Radiação/efeitos dos fármacos , Calicivirus Felino/efeitos dos fármacos , Calicivirus Felino/efeitos da radiação , Citrus/química , Relação Dose-Resposta a Droga , Relação Dose-Resposta à Radiação , Inocuidade dos Alimentos , Vaccinium macrocarpon/química
5.
J Sci Food Agric ; 100(6): 2638-2647, 2020 Apr.
Artigo em Inglês | MEDLINE | ID: mdl-31994185

RESUMO

BACKGROUND: Rice bran is a nutrient-dense and resource-rich byproduct produced from the rice milling. The limitation of rice bran utilization is mainly caused by oxidative deterioration. Improvement of stability to prolong rice bran shelf-life has thus become an urgent requirement. RESULTS: The present study aimed to determine the characteristics of infrared radiation heat treatment of rice bran (IRRB) and raw rice bran stored under different temperatures. The effects of heating and storage time on physicochemical, microbial, storage stability and structural properties were investigated. Additionally, the prediction model for the shelf-life of rice bran was established based on free fatty acids and the peroxide value by fitting the curve of bran lipid oxidation. The results obtained demonstrated that infrared radiation heating at 300 °C for 210 s resulted in decreased lipase activity and peroxidase activity of 73.05% and 81.50%, respectively. The free fatty acids and peroxide value of IRRB stored at 4 and 25 °C for 8 weeks were only reached at 2.35% and 3.17% and 2.53 and 3.64 meq kg-1 , respectively. The shelf-life prediction model showed the the shelf-life of infrared radiation-treated samples increased to 71.6 and 25.8 weeks under storage at 4 and 25 °C, respectively. CONCLUSION: The stabilizing process could effectively suppress microbial growth and had no prominent effect on the physicochemical and microstructure properties of rice bran and, simultaneously, storage life was greatly extended. © 2020 Society of Chemical Industry.


Assuntos
Irradiação de Alimentos/métodos , Armazenamento de Alimentos , Raios Infravermelhos , Oryza , Fibras na Dieta/análise , Fibras na Dieta/microbiologia , Ácidos Graxos não Esterificados/análise , Conservação de Alimentos , Peroxidação de Lipídeos
6.
Food Sci Technol Int ; 25(8): 649-658, 2019 Dec.
Artigo em Inglês | MEDLINE | ID: mdl-31213080

RESUMO

Aspergillus flavus is the potential pathogenic mold in red pepper powder (Capsicum annuum L.) and gochujang (red pepper paste), which can produce mycotoxins. This study investigated the effects of gamma ray, e-beam, and X-ray irradiation on the reduction of A. flavus on red pepper powder and gochujang and physicochemical and sensory quality changes. Gamma ray and e-beam at 3.5 kGy reduced A. flavus effectively (>4 log), without deteriorating the physicochemical quality. Same dose of X-ray did not cause any deterioration of the physicochemical quality. However, reduction effect of A. flavus in red pepper powder and gochujang by 3.5 kGy X-ray was under 2 log. Further, sensory quality analysis showed no significant difference in color, appearance, texture, and overall acceptability after three irradiations. However, flavor changes of red pepper powder and gochujang after three irradiations were mentioned by panelists. In this study, gamma ray and e-beam irradiation were effective in eliminating A. flavus present in red pepper powder and gochujang, but X-ray irradiation was not effective. The results indicate gamma ray and e-beam are effective in controlling microorganisms present in powdery or paste foods, but the X-ray was not effective.


Assuntos
Aspergillus flavus/efeitos da radiação , Capsicum/efeitos da radiação , Elétrons , Irradiação de Alimentos/métodos , Raios gama , Pós/efeitos da radiação , Capsicum/química , Capsicum/microbiologia , Contagem de Colônia Microbiana , Cor , Relação Dose-Resposta à Radiação , Contaminação de Alimentos/prevenção & controle , Manipulação de Alimentos/métodos , Microbiologia de Alimentos , Qualidade dos Alimentos , Concentração de Íons de Hidrogênio , Pós/análise , Paladar , Raios X
7.
Int J Food Microbiol ; 305: 108238, 2019 Sep 16.
Artigo em Inglês | MEDLINE | ID: mdl-31174101

RESUMO

Bacteria of the genus Alicyclobacillus pose serious quality problems for the juice processing industries that have sought effective alternatives for its control. The present study evaluated the effect of UV-C radiation on the reduction of spores and biofilm formation of Alicyclobacillus spp. on stainless steel and rubber surfaces using industrialized orange juice as a culture medium. Four reference Alicyclobacillus spp. species and different UV-C dosages were investigated. After exposed for 20 min (16.8 kJ/m2) to UV-C, the spores of Alicyclobacillus acidoterrestris, Alicyclobacillus herbarius, and Alicyclobacillus cycloheptanicus decreased drastically more of 4 log CFU/mL, with counts below the detection limit of the method (<1.7 log CFU/mL), while the Alicyclobacillus acidocaldarius spores were more sensitive to UV-C, once this spore reduction was observed within 15 min (12.6 kJ/m2). Morphological changes in the Alicyclobacillus acidoterrestris spores were observed by scanning electron microscopy. A reduction of biofilm formation was observed for all UV-C treatments, and the higher reductions (approximately 2 log CFU/mL) were found for the Alicyclobacillus acidocaldarius species after 30 min (26.2 kJ/m2), on the stainless steel and rubber surfaces. The results suggest that UV-C can be used to reduce the biofilm formation and could be a promising alternative for controlling Alicyclobacillus spp. spores in industrialized orange juice.


Assuntos
Alicyclobacillus/efeitos da radiação , Biofilmes/efeitos da radiação , Citrus sinensis/química , Irradiação de Alimentos/métodos , Sucos de Frutas e Vegetais/microbiologia , Esporos Bacterianos/efeitos da radiação , Alicyclobacillus/classificação , Alicyclobacillus/crescimento & desenvolvimento , Alicyclobacillus/isolamento & purificação , Manipulação de Alimentos/instrumentação , Microbiologia de Alimentos , Esporos Bacterianos/crescimento & desenvolvimento , Aço Inoxidável/análise , Raios Ultravioleta
8.
J Sci Food Agric ; 99(11): 5229-5238, 2019 Aug 30.
Artigo em Inglês | MEDLINE | ID: mdl-31021408

RESUMO

BACKGROUND: In this study, fresh pistachio was exposed to UV-C irradiation (2.1 and 4.5 kJ m-2 ) in a rotating cylindrical system with seven germicidal UV-C lamps and immediately packed in perforated and non-perforated polyethylene terephthalate (PET). The fruit were evaluated for weight loss, total phenolic content, enzyme activities, color indices (L*, a*, b* and browning index), and microbial counts during 35 days of storage at 4 °C. RESULTS: UV-C treatment caused a significant decrease (P < 0.05) in the weight loss of fresh pistachios compared to the control. The activity of catalase and peroxidase enzymes was significantly higher (P < 0.05) in irradiated samples packed in non-perforated PET in comparison to those of untreated samples. Irradiation did not inhibit the activity of polyphenol oxidase in treated samples, although a slight decrease in polyphenol oxidase activity was observed in irradiated samples compared to control. The fruit treated with 2.1 kJ m-2 of UV-C and the control packed in non-perforated PET were lighter (L*), redder (a*), and less yellow (b*) compared to 4.5 kJ m-2 treated samples. Furthermore, a dose of 4.5 kJ m-2 UV-C significantly decreased sensory attributes of fresh pistachios compared to the other irradiation level and control. CONCLUSION: UV-C irradiation at a dose of 2.1 kJ m-2 and packing in non-perforated PET are recommended for fresh pistachio preservation based on the physicochemical, microbial, and sensory parameters. © 2019 Society of Chemical Industry.


Assuntos
Irradiação de Alimentos/métodos , Embalagem de Alimentos/métodos , Frutas/efeitos da radiação , Pistacia/química , Catecol Oxidase/análise , Embalagem de Alimentos/instrumentação , Armazenamento de Alimentos , Frutas/química , Humanos , Pistacia/efeitos da radiação , Proteínas de Plantas/análise , Controle de Qualidade , Paladar , Raios Ultravioleta
9.
J Sci Food Agric ; 99(9): 4474-4481, 2019 Jul.
Artigo em Inglês | MEDLINE | ID: mdl-30868583

RESUMO

BACKGROUND: The isolated application of high hydrostatic pressure (HHP) and ultraviolet-C (UV-C) radiation may induce physicochemical changes, thus jeopardizing fish quality attributes during refrigerated storage, which could be minimized by the use of synergistic treatments. Therefore, this study investigated the combined effect of UV-C at 0.103 ± 0.002 J cm−2 and HHP at 220 MPa for 10 min on quality parameters of tilapia fillets stored at 4 °C for 15 days. RESULTS: HHP and UV-C+HHP showed higher myoglobin concentration, higher metmyoglobin-reducing activity, and lower a* than control and the UV-C treatment (P < 0.05), reaching 2.49, 2.58, 1.62, 1.54 mg myoglobin g−1 , 13.30, 13.81, 2.29, 2.29%, and 2.32, 2.58, 4.29, 3.98 respectively on the last day of storage. UV-C, HHP, and UV-C+HHP increased water-holding capacity and decreased the pH levels, hardness, and chewiness (P < 0.05). HHP retarded lipid oxidation and UV-C increased protein oxidation compared with control (P < 0.05), achieving 0.81 and 2.80 mg malondialdehyde per kilogram of fish tissue, and 5.50 and 4.15 nmol carbonyl per milligram of protein respectively on 15th day of the storage. CONCLUSION: Both technologies (alone or together) enhanced texture parameters and water-holding capacity. UV-C did not induce color changes and lipid oxidation, whereas HHP showed high potential to prevent meat discoloration, lipid oxidation, and protein oxidation. In addition, this combination of methods could be an alternative to prevent protein oxidation induced by UV-C.


Assuntos
Produtos Pesqueiros/análise , Irradiação de Alimentos/métodos , Conservação de Alimentos/métodos , Animais , Cor , Produtos Pesqueiros/efeitos da radiação , Conservação de Alimentos/instrumentação , Pressão Hidrostática , Oxirredução , Tilápia , Raios Ultravioleta
10.
Braz J Microbiol ; 50(1): 231-235, 2019 Jan.
Artigo em Inglês | MEDLINE | ID: mdl-30637655

RESUMO

Excess sodium in food is associated with an increased risk of noncommunicable diseases. Faced with this reality, consumers are becoming aware of the importance of eating foods with reduced sodium content. This work assesses the effects of partial replacement of NaCl by KCl on the microbiological and physicochemical characteristics of uncooked lamb sausage treated with different doses of gamma radiation. Analyses of thermotolerant coliforms, sulfite-reducing Clostridium, Salmonella sp., protein, moisture, ash, lipids, pH, water activity, and color were performed. The results showed that the formulations with reduced sodium content did not differ significantly concerning microbial growth and met the identity and quality standards considering its physicochemical characteristics. Also, irradiation is efficient as a microbiological control method, and the ± 3-kGy dose was the most effective to eliminate pathogenic bacteria present in the sausage formulation prepared with reduced sodium content, inducing less intense changes in physicochemical parameters.


Assuntos
Irradiação de Alimentos/métodos , Produtos da Carne/efeitos da radiação , Sódio/análise , Animais , Bactérias/classificação , Bactérias/genética , Bactérias/isolamento & purificação , Culinária , Contaminação de Alimentos/análise , Contaminação de Alimentos/prevenção & controle , Produtos da Carne/análise , Produtos da Carne/microbiologia , Ovinos
11.
J Sci Food Agric ; 99(1): 136-142, 2019 Jan 15.
Artigo em Inglês | MEDLINE | ID: mdl-29797728

RESUMO

BACKGROUND: Cordyceps militaris is a medicine and food dual-purpose mushroom extensively cultivated and consumed in East and Southeast Asia for centuries. However, it has an extremely short shelf life of 3-4 days at room temperature. C. militaris was pasteurized for 10, 20, and 30 min by radio-frequency (RF) at an electrode gap of 20 mm. The effect of RF heating on the microbial load, color, texture, and flavor attributes of C. militaris was evaluated and compared with those sterilized by conventional high-pressure steam. RESULTS: RF heating contributed to good heating uniformity, uniform temperature distribution, and significant decrease in total microbial load. C. militaris heated by RF exhibited unnoticeable total color difference compared with unpasteurized ones, while those sterilized by high-pressure steam presented undesired and unacceptable browning. Insignificant differences in hardness and chewiness were observed after RF heating. Improvements in mushroom-like flavor occurred after 10 and 20 min of RF heating. CONCLUSION: This study suggests that RF heating for pasteurization of edible mushrooms has promising prospects. Evaluation of RF heating on the taste and nutritional characteristics of edible mushrooms is needed in future work. © 2018 Society of Chemical Industry.


Assuntos
Cordyceps/química , Cordyceps/efeitos da radiação , Irradiação de Alimentos/métodos , Cor , Microbiologia de Alimentos , Armazenamento de Alimentos , Humanos , Controle de Qualidade , Ondas de Rádio , Paladar
12.
Food Chem ; 271: 747-752, 2019 Jan 15.
Artigo em Inglês | MEDLINE | ID: mdl-30236740

RESUMO

Sauvignon blanc grapes were exposed to an ultra-violet (UV) light source post-hand harvest (whole bunches) or post-machine harvest. The thiol precursors S-3-(hexan-1-ol)-l-cysteine (Cys-3MH) and S-3-(hexan-1-ol)-l-glutathione (GSH-3MH) were quantified in the juices before and after UV treatment. Results showed that irradiation of the grapes with UV light had little to no effect on the thiol precursors. Wines were fermented from the corresponding juices and 18 aroma compounds were quantified. Differences were found between UV treatments of the wines for 3-mercaptohexanol, hexan-1-ol, ethyl butanoate, ethyl hexanoate, ethyl octanoate and phenylethyl alcohol. However, these changes were not significant (p < 0.05) for both grape media trialled. Future studies involving larger sample sizes and replicate numbers should be completed in order to ascertain any changes in aroma chemistry as a result of UV light application to grapes postharvest.


Assuntos
Irradiação de Alimentos/métodos , Compostos de Sulfidrila/efeitos da radiação , Raios Ultravioleta , Vitis/química , Vinho/análise , Odorantes
13.
Food Chem ; 270: 113-122, 2019 Jan 01.
Artigo em Inglês | MEDLINE | ID: mdl-30174024

RESUMO

The effects of a novel technology utilizing a simultaneous combination of Ultraviolet-C radiation and ultrasound energy postharvest treatment on tomato bioactive compounds during 28 days' storage period was investigated by varying Ultraviolet-C radiation intensities of 639.37 or 897.16 µW/cm2 at a constant ultrasound intensity of 13.87 W/L from a 40 kHz-1 kW transducer. A minimal treatment time of 240 s at Ultraviolet-C dosage of 2.15 kJ/m2 was observed to provoke a considerable increase in bioactive compounds content, proportionated to treatment time. Although treatment led to temperature increase in the system reaching 39.33 °C due to heat generation by ultrasonic cavitation, the extractability and biosynthesis of phytochemicals were enhanced resulting in 90%, 30%, 60%, 20%, and 36% increases in lycopene, total phenols, vitamin C, hydrophilic and lipophilic antioxidant activities respectively. Results present the potential use of the combined non-thermal technologies as post-harvest treatment to improve bioactive compounds and antioxidant activity during storage.


Assuntos
Irradiação de Alimentos/métodos , Armazenamento de Alimentos/métodos , Solanum lycopersicum/metabolismo , Antioxidantes/metabolismo , Ácido Ascórbico , Armazenamento de Alimentos/normas , Solanum lycopersicum/efeitos da radiação , Fenóis , Ultrassom
14.
Food Microbiol ; 76: 374-381, 2018 Dec.
Artigo em Inglês | MEDLINE | ID: mdl-30166163

RESUMO

The present work was performed to evaluate the potential of electron beam ionizing radiation for the inactivation of three psychrophilic spore forming bacteria (Bacillus mycoides, Bacillus weihenstephanensis and Psychrobacillus psychrodurans) isolated from ready-to-eat brown crab (Cancer pagurus). Inactivation curves for the three spores were performed in both types of crab meat, brown and white. Also the effect of pH and water activity (aw) on the lethal efficacy of ionizing radiation, for the three different psychrophilic spore forming bacteria, was evaluated. The effects of pH, aw and their possible interactions were assessed in citrate-phosphate buffers of different pH, ranging between 7 and 4, and aw, ranging from <0.99 to 0.80. A reduction of aw increased the spores resistance between >0.99 and 0.90, while an aw reduction from 0.90 to 0.80 had a minor impact on their resistance. In contrast to aw, the effect of pH showed a greater variability depending on the spore species. While pH did not affect the resistance of B. weihenstephanensis at any aw, B. mycoides showed slightly higher resistance at pH 5.5 at aw of 0.90 and 0.80. pH showed a significant effect on the resistance of P. psychrodurans. For the two types of crab meat, slightly differences were observed in 6D values. B. weihenstephanensis was the most resistant, requiring 7.3-7.6 kGy to inactivate 6 Log10-cycles of this spore forming bacterium, while for B. mycoides and P. psychrodurans 6.1-6.3 and 5.4-5.3 kGy respectively were necessary to reach the same inactivation level in crab meat. An agreement between spore resistance in crab meats and lab media, with similar characteristics in pH and aw, was also observed. The results obtained in this research demonstrated the potential for ionizing radiation to achieve an appropriate inactivation level of spores naturally present in brown crab with the application of doses lower than 10 kGy.


Assuntos
Bactérias/efeitos da radiação , Braquiúros/microbiologia , Irradiação de Alimentos/métodos , Carne/microbiologia , Frutos do Mar/microbiologia , Esporos Bacterianos/efeitos da radiação , Animais , Bactérias/genética , Bactérias/crescimento & desenvolvimento , Bactérias/isolamento & purificação , Radiação Ionizante , Frutos do Mar/análise , Esporos Bacterianos/genética , Esporos Bacterianos/crescimento & desenvolvimento , Esporos Bacterianos/isolamento & purificação
15.
Foodborne Pathog Dis ; 15(9): 538-543, 2018 09.
Artigo em Inglês | MEDLINE | ID: mdl-29851358

RESUMO

Nonendospore-forming pathogenic bacteria in the long-term survival (LTS) phase can remain viable for months or years and may show reduced susceptibility to various antimicrobial interventions. In the present study, we investigated the response of LTS phase Salmonella enterica serovar Typhimurium (ATCC 14028) to ultraviolet (UV) radiation in 0.85% (w/v) saline and apple juice and the extent of sublethal injury in LTS phase survivors. The LTS-phase Salmonella Typhimurium cells were cultured at 35°C for 14 days in tryptic soy broth with 0.6% (w/v) yeast extract (TSBYE). Exponential- and stationary-phase cells, cultured in TSBYE (35°C) for 2.5 and 18 h, respectively, served as control samples. Cells (107 CFU [colony-forming unit]/mL) from each physiological state were exposed to UV light in saline (80 µW/cm2) and apple juice (1500 µW/cm2). The Salmonella Typhimurium survivors were plated for enumeration on either tryptic soy agar with 0.6% yeast extract or xylose-lysine-tergitol 4 (XLT4) agar and colonies counted after incubation (35°C, 24 h). Of all the growth phases tested, LTS phase cells were consistently impacted the least by UV treatment (p < 0.05). In saline, D-values of exponential, stationary, and LTS Salmonella Typhimurium were 0.35, 0.38, and 0.49 min, respectively. D-values in apple juice at pH 3.63 and pH 5.65 were 2.52, 3.19, and 3.57 min and 3.24, 3.50, and 4.18 min, respectively. UV radiation (80 µW/cm2) of Salmonella Typhimurium in saline for 2.5 min reduced the number of exponential- and stationary-phase cells by ∼7.19 and 6.30 log10 CFU/mL, respectively. In contrast, LTS cells were only reduced by 5.08 log10 CFU/mL. Among the three physiological states, LTS phase cells had the least sublethal injury in the surviving population (p < 0.05). These results indicate that the LTS state cross-protects Salmonella Typhimurium against UV radiation and should be considered in determination of the UV radiation D-value for this pathogen.


Assuntos
Irradiação de Alimentos/métodos , Sucos de Frutas e Vegetais/microbiologia , Tolerância a Radiação , Salmonella typhimurium/efeitos da radiação , Contagem de Colônia Microbiana , Microbiologia de Alimentos , Malus , Solução Salina , Salmonella typhimurium/crescimento & desenvolvimento , Temperatura , Fatores de Tempo
16.
Appl Radiat Isot ; 139: 224-230, 2018 Sep.
Artigo em Inglês | MEDLINE | ID: mdl-29852404

RESUMO

Fungal infection is inevitable in the cultivation and storage process of soybean. Gamma irradiation is an effective method to control fungal growth and inactivate mycotoxins. The effects of gamma irradiation and fungal damage on the number of fungi, aflatoxin B1 content, proximate composition of soybeans, and quality of soybean oil (acid value, peroxide value, iodine value, fatty acid profile, tocopherols content, and oxidation stability) were investigated in this work. Growth of fungi caused some changes in proximate composition of soybean and qualities of soybean oil. However, the changes depended on the damage extent of soybeans. No significant change was found for the soybeans incubated for 30 days (moderately fungi-damaged). Gamma irradiation could completely eliminate the fungi and greatly reduce the content of aflatoxin B1 in soybeans at 10 kGy. For soybeans incubated for 30 days, there were no significant changes in the quality attributes, tocopherols content and oxidation stability of oil when the gamma irradiation dose was less than 20 kGy. Gamma irradiation is a promising method to improve the safety and economy of moderately fungi-damaged soybean used for feedstuff.


Assuntos
Aflatoxina B1/análise , Aflatoxina B1/efeitos da radiação , Irradiação de Alimentos/métodos , Glycine max/química , Glycine max/efeitos da radiação , Óleo de Soja/química , Óleo de Soja/efeitos da radiação , Ácidos Graxos/análise , Contaminação de Alimentos/prevenção & controle , Microbiologia de Alimentos , Fungos/isolamento & purificação , Fungos/efeitos da radiação , Raios gama/uso terapêutico , Humanos , Oxirredução , Tocoferóis/análise
17.
Int J Food Microbiol ; 275: 8-16, 2018 Jun 20.
Artigo em Inglês | MEDLINE | ID: mdl-29604491

RESUMO

Outbreaks of foodborne illness associated with berries often involve contamination with hepatitis A virus (HAV) and norovirus but also bacteria such as Escherichia coli O157:H7 and parasites such as Cyclospora caytanensis. We evaluated the applicability of UV-C to the inactivation of pathogens on strawberries, raspberries and blueberries. Our three-step approach consisted of assessing the chemical safety of UV-C-irradiated berries, evaluating the sensory quality after UV-C treatment and finally studying the inactivation of the target microorganisms. Treatments lasting up to 9 min (4000 mJ cm-2) did not produce detectable levels of furan (<5 µg/kg), a known photolysis product of fructose with genotoxic activity and thus were assessed to be toxicologically safe. No effect on taste or appearance was observed, unless treatment was excessively long. 20 s of treatment (an average fluence of ~ 212 mJ cm-2) reduced active HAV titer by >1 log10 unit in 95% of cases except on frozen raspberries, while 120 s were required to inactivate murine norovirus to this extent on fresh blueberries. The mean inactivation of HAV and MNV was greater on blueberries (2-3 log10) than on strawberries and raspberries (<2 log10). MNV was more sensitive on fresh than on frozen berries, unlike HAV. Inactivation of Salmonella, E. coli O157:H7 and Listeria monocytogenes was poor on all three berries, no treatment reducing viable counts by >1 log10 unit. In most matrices, prolonging the treatment did not improve the result to any significant degree. The effect was near its plateau after 20 s of treatment. These results provide insight into the effectiveness of UV-C irradiation for inactivating bacterial and viral pathogens and surrogates on fresh and frozen berries having different surface types, under different physical conditions and at different levels of contamination. Overall they show that UV-C as single processing step is unsuitable to inactivate significant numbers of foodborne pathogens on berries.


Assuntos
Mirtilos Azuis (Planta)/microbiologia , Irradiação de Alimentos/métodos , Doenças Transmitidas por Alimentos/prevenção & controle , Fragaria/microbiologia , Frutas/microbiologia , Viabilidade Microbiana/efeitos da radiação , Rubus/microbiologia , Animais , Escherichia coli O157/efeitos da radiação , Microbiologia de Alimentos , Congelamento , Vírus da Hepatite A/efeitos da radiação , Listeria monocytogenes/efeitos da radiação , Norovirus/efeitos da radiação , Salmonella/efeitos da radiação , Raios Ultravioleta
18.
J Sci Food Agric ; 98(14): 5444-5449, 2018 Nov.
Artigo em Inglês | MEDLINE | ID: mdl-29675926

RESUMO

BACKGROUND: High microbial load is a serious concern in terms of the health-related safety of products of animal origin. In this study, the microbial loads of commercial frozen duck-meat products, including bone-in whole raw, boneless sliced raw, and boneless whole smoked, were investigated for pathogenic contamination. The application of electron beam irradiation was also investigated. RESULTS: The samples revealed a serious microbial threat (102 -105 CFU g-1 for total aerobic bacteria and positive for foodborne pathogens), which required effective decontamination technology. Electron-beam irradiation (0, 1, 3, and 7 kGy) could potentially improve the hygienic quality of duck-meat samples. The D10 values for Listeria monocytogenes and Salmonella Typhi were 0.47 and 0.51 kGy, respectively. A direct epifluorescent filter technique and aerobic plate count (DEFT/APC) method was used for screening, while electron-spin resonance (ESR) spectroscopy and gas chromatography with mass spectrometry were effective as confirmatory techniques to identify radiation-induced markers in frozen duck meat. CONCLUSION: Electron-beam irradiation has the potential to ensure the microbial safety and hygienic quality of commercial duck meats. Identification of the samples for their irradiation history was also possible using radiation-induced detection markers, including the DEFT/APC, hydroxyapatite ESR radicals, and hydrocarbons. © 2018 Society of Chemical Industry.


Assuntos
Contaminação de Alimentos/prevenção & controle , Irradiação de Alimentos/métodos , Produtos da Carne/microbiologia , Animais , Patos , Contaminação de Alimentos/análise , Irradiação de Alimentos/instrumentação , Microbiologia de Alimentos , Listeria monocytogenes/crescimento & desenvolvimento , Listeria monocytogenes/efeitos da radiação , Produtos da Carne/efeitos da radiação , Controle de Qualidade , Salmonella typhi/crescimento & desenvolvimento , Salmonella typhi/efeitos da radiação
19.
Food Res Int ; 102: 738-747, 2017 12.
Artigo em Inglês | MEDLINE | ID: mdl-29196007

RESUMO

Ultraviolet light type C (UV-C) was studied as a tool to increase enzymatic and non-enzymatic antioxidant defenses and phytochemical levels in 'Isabel' grapes (Vitis labrusca×Vitis vinifera L.). Grapes were exposed to 0, 0.5, 1.0, 2.0, or 4.0kJm-2 UV-C and stored for 1, 3, or 5days post-treatment. One day after UV-C irradiation, the activities of grape antioxidant enzymes and thiols were increased, especially at 1.0 and 2.0kJm-2. These doses increased total phenolic content by almost 20%, while 0.5 and 4.0kJm-2 had no effects. Total monomeric anthocyanin content was increased by >35% by UV-C at 1.0kJm-2; however, anthocyanin profile was unchanged. Grape skin antioxidant capacity was also improved by UV-C irradiation. The 1.0kJm-2 UV-C was considered the hormetic dose. Postharvest UV-C had an elicitor effect on 'Isabel' grapes, positively impacting the antioxidant capacity and phytochemical content.


Assuntos
Antioxidantes/análise , Irradiação de Alimentos/métodos , Vitis , Antocianinas/análise , Relação Dose-Resposta à Radiação , Frutas/química , Frutas/enzimologia , Hibridização Genética , Fenóis/análise , Compostos Fitoquímicos/análise , Compostos de Sulfidrila/análise , Raios Ultravioleta
20.
Hig. aliment ; 31(274/275): 26-27, 30/12/2017.
Artigo em Português | LILACS | ID: biblio-880003

RESUMO

A jabuticaba é uma fruta tropical, tipicamente brasileira, sendo originária da região centro-sul. Popularmente apreciada por suas características sensoriais in natura, mas também pela utilização em produtos processados como geleias, licores e bebidas fermentadas (SASSO; CITADIN; DANNER, 2010). Apesar da designação vinho referir- -se segundo a Lei nº 7.678, de 08 de novembro de 1988 (BRASIL, 1988) como "bebida proveniente da fermentação alcoólica de mosto de uva sã, fresca e madura", é permitido pela Portaria Nº 64, de 23 de abril de 2008 a produção de vinhos de outras frutas (BRASIL, 2008), desde que se indique o nome da fruta no rótulo após a palavra vinho, como por exemplo vinho de jabuticaba.(AU)


Assuntos
Humanos , Vinho/análise , Bebidas Alcoólicas/análise , Irradiação de Alimentos/métodos , Bebidas Alcoólicas/normas , Fermentação
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