RESUMO
CASE DESCRIPTION: 2 male and 3 female adult bearded dragons (Pogona vitticeps) were evaluated at the North Carolina State University College of Veterinary Medicine's Exotic Animal Medicine Service between September 2018 and October 2019 because of severe lymphocytosis. CLINICAL FINDINGS: All 5 bearded dragons had nonspecific clinical signs, including lethargy, poor appetite, ocular discharge, and weight loss. Clinicopathologic testing revealed extremely high lymphocyte counts with morphological findings consistent with lymphocytic leukemia. TREATMENT AND OUTCOME: All 5 patients were treated with lomustine, prednisolone, and antimicrobials. In addition, 1 or 2 doses of L-asparaginase were administered when the drug was available. Partial remission was achieved in all 5 patients. One patient, after disease progression was documented, was treated with cyclophosphamide and achieved a second partial remission. One of the 5 patients was still alive and continuing to receive chemotherapy at the time of final follow-up 244 days after the initial diagnosis. Survival times (ie, times from initial diagnosis to euthanasia) for the other 4 patients were 57, 157, 330, and 416 days. CLINICAL RELEVANCE: The present report represented the first description of lomustine as a primary chemotherapeutic agent for the treatment of lymphocytic leukemia in bearded dragons and provided information on response to treatment, adverse effects, and survival times.
Assuntos
Leucemia Linfoide , Lagartos , Animais , Feminino , Leucemia Linfoide/veterinária , Masculino , North CarolinaRESUMO
BACKGROUND: Differentiation between neoplastic and reactive lymphocytic proliferations can be challenging in cats. PCR for antigen receptor rearrangements (PARR) testing is a useful diagnostic tool to assess clonality of a lymphoid population. Previous feline PARR studies evaluated clonality of complete immunoglobulin heavy chain V-D-J (IGH-VDJ) and T-cell receptor gamma (TRG) gene rearrangements. OBJECTIVES: We aimed to evaluate the sensitivity and specificity of feline PARR primers targeting complete IGH-VDJ and TRG rearrangements, as well as incomplete IGH-DJ, kappa deleting element (Kde), and immunoglobulin lambda light chain (IGL) gene rearrangements in defined feline neoplasms and nonneoplastic controls. METHODS: Fluorescently labeled PCR primers were designed to amplify complete IGH-VDJ, incomplete IGH-DJ, Kde, IGL, and TRG gene rearrangements in two multiplexed PCR reactions, and PCR products were analyzed by fragment analysis. Fresh tissue samples from 12 flow cytometrically confirmed B-cell lymphomas, 26 cytologically confirmed gastric and renal lymphomas of presumed B-cell origin, 30 flow cytometrically confirmed T-cell leukemias, and 11 negative control cats were tested. RESULTS: Using four immunoglobulin primer sets (IGH-VDJ, IGH-DJ, Kde, and IGL), clonal immunoglobulin rearrangements were detected in 87% (33/38) of the presumed B-cell neoplasms. The IGH-VDJ reaction alone only detected clonality in 50% (19/38) of these cases. TRG rearrangements were clonal in 97% (29/30) of the T-cell leukemia cases. All negative control samples had polyclonal immunoglobulin and TRG rearrangements. CONCLUSIONS: The PARR assay developed in this study is useful for assessing clonality in feline lymphoid neoplasms. Clonality assessment of incomplete IGH-DJ, Kde, and IGL rearrangements helped identify clonal B-cell neoplasms not detected with complete IGH-VDJ PARR alone.
Assuntos
Doenças do Gato/diagnóstico , Rearranjo Gênico , Leucemia Linfoide/veterinária , Animais , Doenças do Gato/genética , Doenças do Gato/patologia , Gatos , Feminino , Cadeias Pesadas de Imunoglobulinas/genética , Cadeias Leves de Imunoglobulina/genética , Leucemia Linfoide/diagnóstico , Leucemia Linfoide/genética , Leucemia Linfoide/patologia , Linfócitos/patologia , Masculino , Receptores de Antígenos/genética , Sensibilidade e EspecificidadeRESUMO
CASE DESCRIPTION: Severe lymphocytosis and leukocytosis were detected during examination of a 10-year-old sexually intact male bat-eared fox (Otocyon megalotis) with regionally extensive alopecia. CLINICAL FINDINGS: A CBC revealed severe leukocytosis (39,100 leukocytes/µL) and marked lymphocytosis (90%). A blood smear consisted predominantly of intermediate-sized lymphocytes and few large lymphocytes, with mild to moderate nuclear atypia. These findings were highly suggestive of chronic lymphocytic leukemia (CLL). Cytologic evaluation of bone marrow aspirates revealed no evidence of overt malignancy, with 10% of all cells identified as small to intermediate-sized mature lymphocytes. TREATMENT AND OUTCOME: Treatment with chlorambucil and prednisone administered orally over a 1.8-year period decreased the leukocyte and lymphocyte counts to within reference intervals with no adverse effects. Although repeated flow cytometry revealed evidence of residual disease, the fox remained free of clinical disease, and WBC counts were within reference intervals for this species. At 22 months after initial evaluation, the fox was euthanized because of debilitating arthritis. No evidence of CLL was detected grossly or histologically during necropsy. CLINICAL RELEVANCE: To the authors' knowledge, this was the first report of CLL in a bat-eared fox and first successful treatment in a nondomestic carnivore. Treatment in accordance with a chemotherapeutic protocol successfully resolved the leukocytosis and lymphocytosis with no serious adverse effects. Description of this fox and the treatment protocol should provide a valuable reference for future cases in this and other nondomestic canine species.
Assuntos
Animais de Zoológico , Clorambucila/uso terapêutico , Raposas , Leucemia Linfoide/veterinária , Prednisona/uso terapêutico , Animais , Antineoplásicos Alquilantes/administração & dosagem , Antineoplásicos Alquilantes/uso terapêutico , Antineoplásicos Hormonais/administração & dosagem , Antineoplásicos Hormonais/uso terapêutico , Clorambucila/administração & dosagem , Leucemia Linfoide/diagnóstico , Leucemia Linfoide/tratamento farmacológico , Masculino , Prednisona/administração & dosagemRESUMO
Lymphoid leukemia of T-cell origin was diagnosed in a male Chinese Box turtle, Cuora flavomarginata, of approximately 25 years of age. The turtle presented with a history of anorexia, open-mouth breathing, and lethargy for one week. The CBC findings included a mildly increased PCV, and severe leukocytosis due to high numbers of atypical cells interpreted to be blasts. The blasts were medium-sized cells with round to pleomorphic nuclei, slightly clumped chromatin, indistinct nucleoli, and scant moderate-to-dark blue cytoplasm with occasional red-to-purple cytoplasmic granulation. Cytochemical and immunohistochemical staining indicated that the neoplastic cells were positive for CD3 and α-naphthyl butyrate esterase (ANBE), leading to the diagnosis of T-cell lymphoid leukemia. Histology of tissues collected at necropsy showed multifocal infiltrations of neoplastic round cells in the liver, spleen, kidneys, testicles, pancreas, thyroid, duodenum, bone marrow, epicardium, and myocardium. Transmission electron microscopy failed to identify viral particles within the neoplastic cells. This article describes the hematologic, histologic, and ultrastructural abnormalities associated with lymphoid leukemia in this turtle, and advanced diagnostic methods used for phenotyping the T-cell origin.
Assuntos
Leucemia Linfoide/veterinária , Tartarugas , Animais , Contagem de Células Sanguíneas/veterinária , Medula Óssea/patologia , Duodeno/patologia , Histocitoquímica/veterinária , Imuno-Histoquímica/veterinária , Rim/patologia , Leucemia Linfoide/patologia , Fígado/patologia , Masculino , Microscopia Eletrônica de Transmissão/veterinária , Miocárdio/patologia , Pâncreas/patologia , Pericárdio/patologia , Baço/patologia , Linfócitos T/patologia , Testículo/patologia , Glândula Tireoide/patologiaRESUMO
Seventeen cases of lymphoid neoplasms in swine were investigated and divided into eight histological types. Cases 1-3 were precursor B lymphoblastic leukemias, which occurred in three piglets from the same dam. Cases 4 and 5 were diagnosed, respectively, as a precursor B lymphoblastic lymphoma and a thymic B cell lymphoma, because there were cytological differences between the lymphomas. These five cases of immature B cell malignancies expressed CD79a and terminal deoxynucleotidyl transferase (TdT). Mature B cell lymphomas were divisible into follicular (case 6), diffuse centroblastic (case 7) and intestinal large B cell (cases 8-11) lymphomas. Unlike in case 7, the neoplastic cells in cases 8-11 showed cytological features intermediate between centroblasts and immunoblasts. The mature lymphomas were characterized by positive immunolabeling for CD79a and cytoplasmic immunoglobulins. A case of thymic γδ T cell lymphoma (case 12) were positive for CD3, CD5, WC1 and TdT. Instead of TdT, perforin was expressed in γδ T cell lymphomas (cases 13-17), whose histological characteristics were epitheliotropism, homing into T cell zones of lymphatic tissues, and cytological atypia and pleomorphism. In the present study, lymphoid neoplasms could be classified into discrete histological types, some of which were considered to be specific for swine.
Assuntos
Leucemia Linfoide/veterinária , Tecido Linfoide/patologia , Linfoma/veterinária , Doenças dos Suínos/patologia , Animais , Feminino , Imuno-Histoquímica/veterinária , Leucemia Linfoide/patologia , Linfoma/patologia , Masculino , SuínosRESUMO
Traditionally, classification of leukaemia in dogs has relied on morphological examination and cytochemical staining patterns, but aberrant cellular morphology and stain uptake often curtails accurate categorization, and historical data based on this classification may be unreliable. Immunophenotyping is now the gold standard for classification of leukaemias. The purpose of this prospective study was to assess the clinical pathological and epidemiological features of a population of dogs with morphologically and immunologically confirmed leukaemia and to compare them within categories: acute and chronic lymphoid leukaemia (ALL and CLL), and acute and chronic myeloid leukaemia (AML and CML). There were 64 cases of morphologically and immunologically confirmed leukaemia: 25 cases of ALL, 17 cases of CLL and 22 cases of AML. Prevalence of B and T immunophenotypes in ALL and CLL was not statistically different. Dogs with AML were significantly younger than those with ALL at presentation (P = 0.04). Golden Retriever dogs in the study population were overrepresented in comparison with a control population of dogs (6/25 ALL cases, 8/64 leukaemia cases). No sex was overrepresented. Dogs with ALL had significantly more severe neutropenia (P = 0.001) and thrombocytopenia (P = 0.002) than those with CLL and had significantly more cytopenias. The severity and numbers of cytopenias seen in ALL and AML were not significantly different. Twenty-one of the leukaemia cases showed one cytopenia, fourteen had two cytopenias and twenty-one cases had pancytopenia. Anaemia was the most common cytopenia seen in isolation (17/21). No dogs had neutropenia without anaemia and/or thrombocytopenia. Total white blood cell counts were not different between the groups. The atypical cell counts within the peripheral blood were significantly higher in ALL than AML; both in isolation and as a percentage of the total white blood cell count (P = 0.03). This study strengthens the hypothesis that acute leukaemias give rise to more profound cytopenias, affecting more cell lines, than chronic leukaemias.
Assuntos
Doenças do Cão/diagnóstico , Leucemia Linfoide/veterinária , Leucemia Mieloide/veterinária , Distribuição por Idade , Animais , Doenças do Cão/epidemiologia , Doenças do Cão/patologia , Cães , Feminino , Leucemia Linfoide/epidemiologia , Leucemia Linfoide/patologia , Leucemia Mieloide/epidemiologia , Leucemia Mieloide/patologia , Masculino , Reino Unido/epidemiologiaRESUMO
This report describes recurrent respiratory distress following vincristine administration to a cat with chronic lymphocytic leukaemia. The cat was treated with a combination of vincristine, chlorambucil and prednisolone with initial success. After approximately 4 months, dyspnoea developed within 6 h of vincristine administration. Emergency therapy was instituted resulting in a full recovery. Further vincristine was administered; dyspnoea was similarly noted after all but one of these treatments. Dyspnoeic episodes were not attributable to alterations in vincristine dose or method of administration. The repeated temporal association was consistent with a suspected adverse drug reaction to vincristine.
Assuntos
Antineoplásicos Fitogênicos/efeitos adversos , Doenças do Gato/induzido quimicamente , Edema Pulmonar/veterinária , Vincristina/efeitos adversos , Animais , Antineoplásicos Fitogênicos/uso terapêutico , Doenças do Gato/tratamento farmacológico , Gatos , Feminino , Leucemia Linfoide/tratamento farmacológico , Leucemia Linfoide/veterinária , Edema Pulmonar/induzido quimicamente , Vincristina/uso terapêuticoAssuntos
Doenças das Aves/diagnóstico , Doenças das Aves/terapia , Falconiformes , Leucemia Linfoide/veterinária , Animais , Antineoplásicos/uso terapêutico , Arsenitos/uso terapêutico , Feminino , Leucemia Linfoide/diagnóstico , Leucemia Linfoide/terapia , Contagem de Leucócitos/veterinária , Compostos de Potássio/uso terapêutico , Prognóstico , Resultado do TratamentoRESUMO
A novel small haemoplasma was detected following cytological examination of blood smears from a splenectomized dog with haemic neoplasia. The 16S rRNA and rnpB genes of the organism were partially sequenced and a phylogenetic tree constructed. The organism was most closely related to the small feline haemoplasma, 'Candidatus Mycoplasma haemominutum' (94 % 16S rRNA gene nucleotide sequence identity; 75 % rnpB) and was only distantly related to Mycoplasma haemocanis (78 % 16S rRNA gene nucleotide sequence identity; 65 % rnpB). As this organism has not been cultured in vitro, the candidate species name 'Candidatus Mycoplasma haematoparvum' is proposed.
Assuntos
Doenças do Cão/microbiologia , Eritrócitos/microbiologia , Infecções por Mycoplasma/veterinária , Mycoplasma/classificação , Animais , DNA Bacteriano/análise , DNA Ribossômico/análise , RNA Polimerases Dirigidas por DNA/genética , Cães , Genes de RNAr , Leucemia Linfoide/complicações , Leucemia Linfoide/veterinária , Dados de Sequência Molecular , Mycoplasma/genética , Mycoplasma/isolamento & purificação , Infecções por Mycoplasma/microbiologia , Filogenia , RNA Ribossômico 16S/genética , Esplenectomia/veterináriaRESUMO
Although lymphoma and leukemia usually can be diagnosed by routine cytology and histology, some cases present a diagnostic challenge for pathologists and clinicians. Often the dilemma lies in determining whether a population of lymphocytes is reactive or neoplastic. We review currently available methods for analyzing lymphocyte populations by immunophenotyping and by identifying clonally rearranged immunoglobulin and T-cell receptor genes and discuss how these tests can be used to clarify such diagnostic dilemmas. We also describe the detection of chromosomal abnormalities and methods on the horizon, such as gene expression profiling, to identify diagnostically useful oncogenes. Finally, we review the emerging concept of transitional neoplastic states, in which reactive lymphocytes transform to neoplastic lymphocytes in the presence of continued antigenic stimulation, such as that caused by infection with Helicobacter pylori. The existence of transitional neoplastic states underscores the need for an array of molecular diagnostic tools that would improve our ability to characterize lymphocyte populations in human and animal patients and enhance early detection of neoplastic lymphocytes such that eradication of the infectious or inflammatory stimulus could lead to cure.
Assuntos
Imunofenotipagem/métodos , Leucemia Linfoide/diagnóstico , Linfocitose/etiologia , Linfoma/diagnóstico , Animais , Infecções Bacterianas/diagnóstico , Infecções Bacterianas/veterinária , Células Clonais , Humanos , Imunofenotipagem/veterinária , Inflamação/diagnóstico , Inflamação/veterinária , Leucemia Linfoide/imunologia , Leucemia Linfoide/veterinária , Ativação Linfocitária , Linfócitos/imunologia , Linfocitose/veterinária , Linfoma/imunologia , Linfoma/veterináriaRESUMO
A 3-year-old sexually intact male Bull Mastiff underwent splenectomy for splenic thrombosis; prior to and after splenectomy, multiple blood transfusions were administered. Two weeks after the procedure, T-cell lymphoproliferative disease was diagnosed. Treatment with prednisone and chlorambucil was initiated, and 2 weeks later, cytologic examination of a blood smear revealed small (0.3 microm), coccoid basophilic bodies on the surface of approximately 70% of the RBCs. Morphologically, these resembled "Candidatus Mycoplasma haemominutum." A polymerase chain reaction assay was used to amplify a partial 16S rRNA sequence in blood obtained from the dog; the product was sequenced and compared with 16S rRNA gene sequences of other hemotropic mycoplasmas. The sequence was 98% homologous to that of "Candidatus M haemominutum", but only 77% homologous to that of M haemocanis and M haemofelis.
Assuntos
Anemia Hemolítica/veterinária , Doenças do Cão/diagnóstico , Leucemia Linfoide/veterinária , Infecções por Mycoplasma/veterinária , Mycoplasma/genética , RNA Ribossômico 16S/genética , Anemia Hemolítica/microbiologia , Animais , DNA Ribossômico/análise , Diagnóstico Diferencial , Doenças do Cão/microbiologia , Cães , Evolução Fatal , Hospedeiro Imunocomprometido , Imunossupressores/uso terapêutico , Leucemia Linfoide/complicações , Leucemia Linfoide/imunologia , Masculino , Mycoplasma/patogenicidade , Infecções por Mycoplasma/microbiologia , Reação em Cadeia da Polimerase/veterinária , Esplenectomia/veterinária , Trombose/cirurgia , Trombose/veterináriaRESUMO
The four major types of leukocytoses are inflammatory, glucocorticoid-associated, catecholamine-associated, and neoplastic. These leukocytoses are distinguished by leukocyte concentrations, microscopic features of leukocytes, and associations with other laboratory data. All laboratory findings need to be interpreted within the context of the case information, including signalment, history, and physical examination findings. Newer assays are being used to differentiate the different forms of leukocyte neoplasia and to distinguish between hyperplastic and neoplastic proliferations.
Assuntos
Doenças do Gato/diagnóstico , Doenças do Gato/etiologia , Doenças do Cão/diagnóstico , Doenças do Cão/etiologia , Leucocitose/veterinária , Animais , Gatos , Cães , Inflamação/complicações , Inflamação/veterinária , Leucemia Linfoide/complicações , Leucemia Linfoide/veterinária , Leucócitos/classificação , Leucocitose/diagnóstico , Leucocitose/etiologia , Linfoma/complicações , Linfoma/veterináriaRESUMO
A 7-year-old, mixed-breed dog was presented for evaluation of a possible lymphocytic leukemia. Results of laboratory testing included thrombocytopenia, large granular lymphocytosis, inverted CD4:CD8 ratio, hyperglobulinemia, and hypoalbuminemia. Results of a tick-borne disease panel indicated a positive immunoglobulin G serum titer (1:2,048) to Ehrlichia canis, supporting exposure to this organism. The dog responded to a combination treatment of doxycycline and prednisone. A review of the literature and novel diagnostic methods that aided in the diagnosis of this case are discussed.
Assuntos
Doenças do Cão/diagnóstico , Ehrlichia canis/imunologia , Ehrlichiose/veterinária , Animais , Antibacterianos/administração & dosagem , Antibacterianos/uso terapêutico , Diagnóstico Diferencial , Doenças do Cão/sangue , Doenças do Cão/tratamento farmacológico , Cães , Doxiciclina/administração & dosagem , Doxiciclina/uso terapêutico , Ehrlichia canis/isolamento & purificação , Ehrlichiose/diagnóstico , Feminino , Imunoglobulina G/análise , Leucemia Linfoide/diagnóstico , Leucemia Linfoide/veterinária , Subpopulações de Linfócitos , Linfocitose/veterinária , Prednisona/administração & dosagem , Prednisona/uso terapêutico , Trombocitopenia/veterináriaRESUMO
Although the diagnosis of canine leukemia and lymphoma in advanced stages is usually uncomplicated, some presentations of the disease can be a diagnostic challenge. In certain situations, lymphoma and leukemia can be difficult to distinguish from a benign reactive proliferation of lymphocytes. Because clonality is the hallmark of malignancy, we have developed an assay that uses the polymerase chain reaction to amplify the variable regions of immunoglobulin genes and T-cell receptor genes to detect the presence of a clonal lymphocyte population. The assay detected clonally rearranged antigen receptor genes in 91% of the 77 dogs with lymphoid malignancy. Of the 24 dogs tested, that were either healthy or had clearly defined conditions not related to lymphoid malignancy, a clonally rearranged antigen receptor gene was found in one (a dog with Ehrlichia canis infection). Gene rearrangement was appropriate for the immunophenotype (immunoglobulin gene rearrangement in B-cell leukemias and T-cell receptor gene rearrangement in T-cell leukemias). Dilution analysis showed that the clonal rearrangement could be detected when 0.1-10% of the DNA was derived from neoplastic cells, depending on the source tissue. Potential applications of this assay include the diagnosis of lymphoma or leukemia in biopsy samples, cavity fluids, fine needle aspirates, bone marrow and peripheral blood; the determination of lineage (B or T cell); staging of lymphoma; and detection of residual disease after chemotherapy.
Assuntos
Doenças do Cão/genética , Rearranjo Gênico da Cadeia gama dos Receptores de Antígenos dos Linfócitos T/genética , Cadeias Pesadas de Imunoglobulinas/genética , Leucemia Linfoide/veterinária , Linfoma/veterinária , Animais , Células Clonais , DNA de Neoplasias/química , DNA de Neoplasias/genética , Doenças do Cão/diagnóstico , Doenças do Cão/imunologia , Doenças do Cão/patologia , Cães , Genes de Imunoglobulinas/genética , Cadeias Pesadas de Imunoglobulinas/metabolismo , Imunofenotipagem , Leucemia Linfoide/diagnóstico , Leucemia Linfoide/genética , Leucemia Linfoide/patologia , Linfoma/diagnóstico , Linfoma/genética , Linfoma/patologia , Reação em Cadeia da Polimerase/métodos , Reação em Cadeia da Polimerase/veterinária , Sensibilidade e EspecificidadeRESUMO
Lympholeukemia has been occurring to an epizootic extent with mass mortality in 1 and 2 yr old madai (= Japanese red sea bream) Pagrus major in the winter season (October-May) in the western regions of Japan since 1975. Diseased fish displayed severe anemia and markedly increased numbers of neoplastic lymphocytoid and lymphoblastoid cells in the blood. Neoplastic cells originated in the splenic lymphatic cells and systemically caused severe metastatic lesions in the heart, liver, kidney, digestive tracts, gills and the lateral musculature. Electron microscopy revealed adeno-like viral particles (78 to 83 nm in diameter) in the nucleus of lymphoblastoid cells which appeared in the early prevalent stage but no viral particles in the lymphocytoid cells or plasmacytoid cells, which subsequently increased in number. In this paper, we describe light and electron microscopic features of neoplasms and neoplastic cells.
Assuntos
Doenças dos Peixes/etiologia , Leucemia Linfoide/veterinária , Animais , Doenças dos Peixes/patologia , Japão , Leucemia Linfoide/patologia , Microscopia Eletrônica , Perciformes , Vírion/ultraestruturaRESUMO
A 6-mo-old male Savannah monitor lizard (Varanus exanthematicus) was presented for lethargy and anorexia of 7 days duration. Physical examination revealed a slightly raised subcutaneous mass (1 cm diameter) in the left scapular area. Fine-needle aspiration cytology of the mass revealed a population of immature, pleomorphic lymphoid cells consistent with lymphosarcoma. A hemogram indicated marked leukocytosis (465,000 cells/microl) characterized by extreme lymphocytosis and many circulating lymphoid blast cells. The lizard was euthanized at the owner's request. Necropsy revealed severe hepatomegaly and multiple raised, ulcerated mural masses in the gastrointestinal tract. There were many raised, poorly demarcated tan foci in all the parenchymal organs. Histopathologic examination confirmed infiltration of all parenchymal organs by neoplastic lymphoid cells. Transmission electron microscopic examination failed to identify viruses within the neoplastic cells. A literature review revealed few reports of squamate leukemia and lymphosarcoma and none in Savannah monitor lizards.
Assuntos
Leucemia Linfoide/veterinária , Lagartos , Linfoma não Hodgkin/veterinária , Animais , Autopsia/veterinária , Biópsia por Agulha/veterinária , Análise Química do Sangue/veterinária , Evolução Fatal , Testes Hematológicos/veterinária , Rim/patologia , Leucemia Linfoide/sangue , Leucemia Linfoide/patologia , Fígado/patologia , Linfoma não Hodgkin/sangue , Linfoma não Hodgkin/patologia , MasculinoRESUMO
This report describes six cases of feline large granular lymphocyte lymphoma identified by light microscopy on the basis of their characteristic azurophilic granulation in Giemsa-stained plastic sections and by electron microscopy on the basis of their typical granules. Although the granules of all the tumor cells were negative for peroxidase activity, they all demonstrated chloroacetate-esterase and acid phosphatase activity. All the tumors reacted with cross-reacting antibodies against the CD3 antigen (epsilon chain) and did not react with a cross-reacting monoclonal antibody directed against epitopes on cytoplasmic domains of the CD20 antigen. Three tumors had a positive reaction with a monoclonal human CD57-like antibody. This is highly suggestive of either a cytotoxic T cell or a natural killer cell origin of the neoplasias. In three cats, although other abdominal organs were affected to a variable extent, the main neoplastic lesions were localized in the gastrointestinal tract and the jejunal lymph nodes. In contrast, in the other three cats, organ involvement was more widespread, affecting the lung (two), myocardium (two), precardiac mediastinum (one), salivary gland (one), and spinal cord (one); in addition, leukemia was present in two of these cats. The data presented indicate that tumors made up of large granular lymphocytes occur more frequently in cats than previously assumed and that they share many characteristic features with specific subtypes of clonal disorders of large granular lymphocytes in humans.
Assuntos
Doenças do Gato/patologia , Leucemia Linfoide/veterinária , Linfoma Difuso de Grandes Células B/veterinária , Fosfatase Ácida/metabolismo , Animais , Complexo CD3/metabolismo , Antígenos CD57/metabolismo , Hidrolases de Éster Carboxílico/metabolismo , Doenças do Gato/metabolismo , Gatos , Grânulos Citoplasmáticos/ultraestrutura , Feminino , Técnicas Imunoenzimáticas/veterinária , Leucemia Linfoide/metabolismo , Leucemia Linfoide/patologia , Linfoma Difuso de Grandes Células B/metabolismo , Linfoma Difuso de Grandes Células B/patologia , Masculino , Estudos RetrospectivosRESUMO
OBJECTIVE: Correlation of immunophenotype with history, anatomical and morphological features of lymphoid neoplasia in the koala. METHODS: Routine necropsies were performed on 51 koalas with suspected lymphoid neoplasia between 1986 and 1997 in New South Wales and Queensland. Immunophenotyping was by an immunoperoxidase method utilising species cross-reactive antibodies raised against human lymphocytes and an antibody raised against koala IgG. Cases were classified according to organs and tissues affected and the morphological features of neoplastic cells. RESULTS: Twenty-six (51%) of the cases were of the T cell immunophenotype, 12 (24%) were of B cell immunophenotype and 13 (25%) did not stain. The age and sex of koalas did not correlate with immunophenotype (P = 0.686 and P = 1.000, respectively). Thirty-two cases were leukaemic and 36 had multiple organ involvement, probably reflecting presentation of koalas at advanced stages of disease. Abdominal tissue involvement was most common (44 cases), followed by nodal (32), atypical (21) and cervicomediastinal (14). The T cell immunophenotype was over-represented among the leukaemic cases (P = 0.013). Generally, the T cell immunophenotype predominated except for many affected atypical tissues. Neoplastic cells were mostly of medium nuclear size with round to oval nuclei. No correlations were found for cell morphology, mitotic index and immunophenotype. CONCLUSION: The prognostic value of an immunophenotypic, anatomical and morphological basis for the classification of lymphoid neoplasia in the koala currently is limited by the need to detect these neoplasms at an early age, the requirement for freshly fixed tissues and the restricted range of available cross-reacting antibodies.