RESUMO
Members of the sucking louse genus Pedicinus are ectoparasites of cercopithecid primates in Africa, Asia, and Gibraltar. Pedicinus gabonensis n. sp. is described on the basis of adult male and female specimens collected from the mandrill (Mandrillus sphinx) in Gabon. The new species is compared morphologically with other members of the genus Pedicinus, and a nuclear elongation factor 1 alpha gene sequence is provided. Host associations and geographical distributions of the 18 previously recognized species of the genus and of P. gabonensis n. sp. are reviewed. Updated identification keys are provided for males and females of all known valid species of Pedicinus.
Assuntos
Anoplura/classificação , Infestações por Piolhos/veterinária , Mandrillus/parasitologia , Doenças dos Macacos/parasitologia , Animais , Anoplura/anatomia & histologia , Anoplura/genética , Anoplura/fisiologia , DNA/química , DNA/isolamento & purificação , Feminino , Gabão/epidemiologia , Infestações por Piolhos/epidemiologia , Infestações por Piolhos/parasitologia , Masculino , Doenças dos Macacos/epidemiologia , Alinhamento de Sequência/veterinária , Análise de Sequência de DNA/veterináriaRESUMO
Here, we determined the staining properties of Balamuthia mandrillaris cysts, and assessed the effect of 2, 6-dichlorobenzonitrile (DCB), a cellulose synthesis inhibitor, and calcofluor white, a brightening agent, on its encystment. Periodic acid-Schiff reagent stained the inner wall intensely and middle and outer walls weakly suggesting that the cyst wall of B. mandrillaris may contain glycans. Furthermore, cysts, but not trophozoites, fluoresced when stained with calcofluor white. Calcofluor white and DCB, a cellulose synthesis inhibitor, inhibited B. mandrillaris encystment. This is the first report suggesting possible glycan biosynthesis in B. mandrillaris encystment, and this pathwaymay provide a potentially useful drug target and help improve treatment.