RESUMO
In this work, we investigate the potential of highly sulfated synthetic glycomimetics to act as inhibitors of viral binding/infection. Our results indicate that both long-chain glycopolymers and short-chain glycooligomers are capable of preventing viral infection. Notably, glycopolymers efficiently inhibit Human Papillomavirus (HPV16) infection in vitro and maintain their antiviral activity in vivo, while the glycooligomers exert their inhibitory function post attachment of viruses to cells. Moreover, when we tested the potential for broader activity against several other human pathogenic viruses, we observed broad-spectrum antiviral activity of these compounds beyond our initial assumptions. While the compounds tested displayed a range of antiviral efficacies, viruses with rather diverse glycan specificities such as Herpes Simplex Virus (HSV), Influenza A Virus (IAV), and Merkel Cell Polyomavirus (MCPyV) could be targeted. This opens new opportunities to develop broadly active glycomimetic inhibitors of viral entry and infection.
Assuntos
Resinas Acrílicas/uso terapêutico , Alcanossulfonatos/uso terapêutico , Antivirais/uso terapêutico , Galactosídeos/uso terapêutico , Manosídeos/uso terapêutico , Infecções por Papillomavirus/tratamento farmacológico , Resinas Acrílicas/síntese química , Alcanossulfonatos/síntese química , Animais , Antivirais/síntese química , Linhagem Celular Tumoral , Feminino , Galactosídeos/síntese química , Humanos , Manosídeos/síntese química , Camundongos Endogâmicos BALB C , Vírus/efeitos dos fármacosRESUMO
Tumor-associated macrophages (TAMs) that exist in tumor microenvironment promote tumor progression and have been suggested as a promising therapeutic target for cancer therapy in preclinical studies. Development of theranostic systems capable of specific targeting, imaging, and ablation of TAMs will offer clinical benefits. Here we constructed a theranostic probe, namely, TPE-Man, by attaching mannose moieties to a red-emissive and AIE (aggregation-induced emission)-active photosensitizer. TPE-Man can specifically recognize a mannose receptor that is overexpressed on TAMs by the sugar-receptor interaction and enables fluorescent visualization of the mannose-receptor-positive TAMs in high contrast. The histologic study of mouse tumor sections further verifies TPE-Man's excellent targeting specificity being comparable with the commercial mannose-receptor antibody. TAMs can be effectively eradicated upon exposure to white light irradiation via a photodynamic therapy effect. To our knowledge, this is the first small molecular theranostic probe for TAMs that revealed combined advantages of low cost, high targeting specificity, fluorescent light-up imaging, and efficient photodynamic ablation.
Assuntos
Compostos de Benzilideno/farmacologia , Macrófagos/efeitos dos fármacos , Manosídeos/farmacologia , Fármacos Fotossensibilizantes/farmacologia , Animais , Compostos de Benzilideno/síntese química , Compostos de Benzilideno/efeitos da radiação , Compostos de Benzilideno/toxicidade , Manosídeos/síntese química , Manosídeos/efeitos da radiação , Manosídeos/toxicidade , Camundongos , Fotoquimioterapia/métodos , Fármacos Fotossensibilizantes/síntese química , Fármacos Fotossensibilizantes/efeitos da radiação , Fármacos Fotossensibilizantes/toxicidade , Ratos Sprague-Dawley , Nanomedicina Teranóstica/métodosRESUMO
The opportunistic Gram-negative bacterium Burkholderia cenocepacia causes lethal infections in cystic fibrosis patients. Multivalent mannoside derivatives were prepared as potential inhibitors of lectin BC2L-A, one of the virulence factors deployed by B. cenocepacia in the infection process. An (α1â2)-thio-linked mannobioside mimic bearing an azide functionalized aglycon was conjugated to different multivalent scaffolds such as propargylated calix[4]arenes, methyl gallate and pentaerythritol by azide-alkyne 1,3-dipolar cycloaddition. The interaction between the glycoclusters and the mannose binding BC2L-A lectin from B. cenocepacia was examined by isothermal microcalorimetry, surface plasmon resonance, inhibition of yeast agglutination and analytical ultracentrifugation.
Assuntos
Burkholderia cenocepacia/química , Lectina de Ligação a Manose/química , Manosídeos/química , Testes de Aglutinação , Calorimetria/métodos , Técnicas de Química Sintética , Ligantes , Lectina de Ligação a Manose/metabolismo , Lectina de Ligação a Manose/farmacologia , Manosídeos/síntese química , Manosídeos/metabolismo , Ressonância de Plasmônio de Superfície , Ultracentrifugação/métodos , Leveduras/efeitos dos fármacosRESUMO
UNLABELLED: The ileal lesions of Crohn's disease (CD) patients are colonized by adherent-invasive Escherichia coli (AIEC) bacteria. These bacteria adhere to mannose residues expressed by CEACAM6 on host cells in a type 1 pilus-dependent manner. In this study, we investigated different antagonists of FimH, the adhesin of type 1 pili, for their ability to block AIEC adhesion to intestinal epithelial cells (IEC). Monovalent and multivalent derivatives of n-heptyl α-d-mannoside (HM), a nanomolar antagonist of FimH, were tested in vitro in IEC infected with the AIEC LF82 strain and in vivo by oral administration to CEACAM6-expressing mice infected with LF82 bacteria. In vitro, multivalent derivatives were more potent than the monovalent derivatives, with a gain of efficacy superior to their valencies, probably owing to their ability to form bacterial aggregates. Of note, HM and the multi-HM glycoconjugates exhibited lower efficacy in vivo in decreasing LF82 gut colonization. Interestingly, HM analogues functionalized with an isopropylamide (1A-HM) or ß-cyclodextrin pharmacophore at the end of the heptyl tail (1CD-HM) exerted beneficial effects in vivo. These two compounds strongly decreased the amount of LF82 bacteria in the feces of mice and that of bacteria associated with the gut mucosa when administered orally at a dose of 10 mg/kg of body weight after infection. Importantly, signs of colitis and intestinal inflammation induced by LF82 infection were also prevented. These results highlight the potential of the antiadhesive compounds to treat CD patients abnormally colonized by AIEC bacteria and point to an alternative to the current approach focusing on blocking proinflammatory mediators. IMPORTANCE: Current treatments for Crohn's disease (CD), including immunosuppressive agents, anti-tumor necrosis factor alpha (anti-TNF-α) and anti-integrin antibodies, focus on the symptoms but not on the cause of the disease. Adherent-invasive Escherichia coli (AIEC) bacteria abnormally colonize the ileal mucosa of CD patients via the interaction of the mannose-specific adhesin FimH of type 1 pili with CEACAM6 mannosylated proteins expressed on the epithelial cell surface. Thus, we decided to develop an antiadhesive strategy based on synthetic FimH antagonists specifically targeting AIEC bacteria that would decrease intestinal inflammation. Heptylmannoside (HM)-based glycocompounds strongly inhibit AIEC adhesion to intestinal epithelial cells in vitro. The antiadhesive effect of two of these compounds of relatively simple chemical structure was also observed in vivo in AIEC-infected CEACAM6-expressing mice and was associated with a reduction in the signs of colitis. These results suggest a new therapeutic approach for CD patients colonized by AIEC bacteria, based on the development of synthetic FimH antagonists.
Assuntos
Aderência Bacteriana/efeitos dos fármacos , Doença de Crohn/microbiologia , Infecções por Escherichia coli/tratamento farmacológico , Escherichia coli/efeitos dos fármacos , Glicoconjugados/uso terapêutico , Manosídeos/uso terapêutico , Adesinas de Escherichia coli , Administração Oral , Animais , Antígenos CD/genética , Antígenos CD/metabolismo , Carga Bacteriana , Moléculas de Adesão Celular/genética , Moléculas de Adesão Celular/metabolismo , Descoberta de Drogas , Células Epiteliais/efeitos dos fármacos , Células Epiteliais/microbiologia , Escherichia coli/crescimento & desenvolvimento , Escherichia coli/fisiologia , Infecções por Escherichia coli/microbiologia , Fezes/microbiologia , Proteínas de Fímbrias/antagonistas & inibidores , Fímbrias Bacterianas/metabolismo , Proteínas Ligadas por GPI/genética , Proteínas Ligadas por GPI/metabolismo , Glicoconjugados/química , Mucosa Intestinal/efeitos dos fármacos , Mucosa Intestinal/metabolismo , Mucosa Intestinal/microbiologia , Intestinos/citologia , Intestinos/microbiologia , Manosídeos/síntese química , Manosídeos/química , Manosídeos/farmacologia , Camundongos , beta-CiclodextrinasAssuntos
Compostos de Anilina/química , Encéfalo/efeitos dos fármacos , Sistemas de Liberação de Medicamentos/métodos , Lipossomos/química , Manose/química , Manosídeos/química , Compostos de Anilina/síntese química , Animais , Antibióticos Antineoplásicos/administração & dosagem , Antibióticos Antineoplásicos/farmacocinética , Barreira Hematoencefálica/fisiologia , Daunorrubicina/administração & dosagem , Daunorrubicina/farmacocinética , Humanos , Lipossomos/síntese química , Manose/síntese química , Manosídeos/síntese química , Tamanho da PartículaRESUMO
Adherent-invasive Escherichia coli (AIEC) have previously been shown to induce gut inflammation in patients with Crohn's disease (CD). We developed a set of mannosides to prevent AIEC attachment to the gut by blocking the FimH bacterial adhesin. The crystal structure of the FimH lectin domain in complex with a lead thiazolylaminomannoside highlighted the preferential position for pharmacomodulations. A small library of analogues showing nanomolar affinity for FimH was then developed. Notably, AIEC attachment to intestinal cells was efficiently prevented by the most active compound and at around 10000-fold and 100-fold lower concentrations than mannose and the potent FimH inhibitor heptylmannoside, respectively. An ex vivo assay performed on the colonic tissue of a transgenic mouse model of CD confirmed this antiadhesive potential. Given the key role of AIEC in the chronic intestinal inflammation of CD patients, these results suggest a potential antiadhesive treatment with the FimH inhibitors developed.
Assuntos
Aderência Bacteriana/efeitos dos fármacos , Doença de Crohn/microbiologia , Escherichia coli/fisiologia , Manosídeos/farmacologia , Adesinas de Escherichia coli/metabolismo , Animais , Antígenos CD/genética , Antígenos CD/metabolismo , Sítios de Ligação , Células CACO-2 , Moléculas de Adesão Celular/genética , Moléculas de Adesão Celular/metabolismo , Colo/efeitos dos fármacos , Colo/metabolismo , Colo/microbiologia , Escherichia coli/metabolismo , Proteínas de Fímbrias/antagonistas & inibidores , Proteínas de Fímbrias/metabolismo , Fímbrias Bacterianas/fisiologia , Proteínas Ligadas por GPI/genética , Proteínas Ligadas por GPI/metabolismo , Interações Hospedeiro-Patógeno/efeitos dos fármacos , Humanos , Intestinos/efeitos dos fármacos , Intestinos/microbiologia , Células Jurkat , Manosídeos/síntese química , Manosídeos/química , Camundongos , Camundongos Transgênicos , Modelos Químicos , Estrutura Molecular , Tiazóis/síntese química , Tiazóis/química , Tiazóis/farmacologiaRESUMO
A series of oligovalent carbohydrate assemblies (ranging from mono- to pentavalent), derived from three structurally different ß-linked or ß-(1â2)-linked mannosides, has been chemically synthesized, and the respective compounds have been biologically evaluated in order to investigate their immunostimulatory properties. The Crich methodology for ß-mannosylation was successfully utilized to introduce the ß-linkages, and a click chemistry protocol was utilized to generate the oligovalent derivatives. A convenient protecting group strategy involving the simultaneous use of both p-methoxybenzyl and benzylidene groups was employed, which allowed a simple and cost-effective global deprotection step. The immunomodulatory properties of the synthesized multivalent mannosides were evaluated by assessing cytokine production in human white blood cell cultures. The Th2-type cytokines interleukin-4 and interleukin-5 (IL-4 and IL-5), the Th1 cytokine interferon-γ (IFN-γ), the Treg cytokine IL-10, and the pro-inflammatory cytokine tumor necrosis factor (TNF) were included in the screening. A single trivalent acetylated mannobiose derivative was identified as a potent inducer of Treg and Th1 immune response, resulting in strong IL-10 and moderate IFN-γ productions dose-dependently, while inducing no Th2 cytokine response. The immunomodulatory properties of this trivalent mannoside were further studied in vitro in allergen (Betâ v)-stimulated human peripheral blood mononuclear cell cultures of birch pollen allergic subjects. Stimulation with birch pollen induced strong IL-4 and IL-5 responses, which could be suppressed by the trivalent acetylated mannobiose derivative. The IL-10 response was also suppressed, whereas the production of IFN-γ was strongly enhanced. The results suggest that the identified lead compound has suppressive effects on the Th2-type allergic inflammatory response and shows potential as a possible lead adjuvant for the specific immunotherapy of allergies.
Assuntos
Adjuvantes Imunológicos/síntese química , Alérgenos/imunologia , Manosídeos/síntese química , Oligossacarídeos/síntese química , Adjuvantes Imunológicos/química , Alérgenos/sangue , Alérgenos/química , Betula/química , Betula/imunologia , Química Click , Citocinas/sangue , Citocinas/imunologia , Humanos , Hipersensibilidade/imunologia , Interferon gama/biossíntese , Interleucina-10/sangue , Interleucina-10/imunologia , Interleucina-4/sangue , Interleucina-4/imunologia , Interleucina-5/sangue , Interleucina-5/imunologia , Leucócitos Mononucleares/efeitos dos fármacos , Leucócitos Mononucleares/imunologia , Masculino , Manosídeos/sangue , Manosídeos/imunologia , Estrutura Molecular , Oligossacarídeos/sangue , Oligossacarídeos/imunologia , Pólen/imunologia , Células Th1/imunologia , Células Th2/imunologia , Fator de Necrose Tumoral alfa/sangue , Fator de Necrose Tumoral alfa/imunologiaRESUMO
A simple and reliable continuous assay for measurement of alpha-mannosidase activity is described and demonstrated for analysis with two recombinant human enzymes using the new substrate resorufin alpha-d-mannopyranoside (Res-Man). The product of enzyme reaction, resorufin, exhibits fluorescence emission at 585 nm with excitation at 571 nm and has a pK(a) of 5.8, allowing continuous measurement of fluorescence turnover at or near physiological pH values for human lysosomal and Drosophila Golgi alpha-mannosidases. The assay performed using recombinant Drosophila Golgi alpha-mannosidase (dGMII) has been shown to give the kinetic parameters K(m) of 200 microM and V(max) of 11 nmol/min per nmol dGMII. Methods for performing the assay using several concentrations of the known alpha-mannosidase inhibitor swainsonine are also presented, demonstrating a potential for use of the assay as a simple method for high-throughput screening of inhibitors potentially useful in cancer treatment.
Assuntos
Corantes Fluorescentes/química , Fluorometria/métodos , Manosídeos/metabolismo , Oxazinas/metabolismo , alfa-Manosidase/metabolismo , Animais , Drosophila , Inibidores Enzimáticos/química , Inibidores Enzimáticos/farmacologia , Complexo de Golgi/enzimologia , Ensaios de Triagem em Larga Escala , Humanos , Cinética , Lisossomos/enzimologia , Manosídeos/síntese química , Manosídeos/química , Oxazinas/síntese química , Oxazinas/química , Proteínas Recombinantes/genética , Proteínas Recombinantes/metabolismo , Especificidade por Substrato , Swainsonina/química , Swainsonina/farmacologia , alfa-Manosidase/genéticaRESUMO
Urinary tract infections caused by uropathogenic Escherichia coli presents a serious communal and nosocomial health problem initiated by bacterial adhesion to the bladder cells. E. coli expresses fimbriae with a mannose-binding adhesin, FimH, at the tip. Heptyl alpha-D-mannoside (HM) is a nanomolar inhibitor of this lectin, preventing adhesion of type 1-piliated E. coli and reducing bacteria levels in a murine cystitis model. Herein, we described the synthesis of multimeric heptyl-mannosides with valencies ranging from one to four by copper-catalyzed azide alkyne cycloaddition (CuAAC). Biological evaluation of the multivalent compounds revealed an increase in potency compared to HM. Inhibition of bladder cell binding highlighted a promising tetravalent derivative with inhibitory concentrations 6000- and 64-fold lower than mannose and HM respectively.
Assuntos
Antibacterianos/síntese química , Aderência Bacteriana/efeitos dos fármacos , Escherichia coli/efeitos dos fármacos , Manosídeos/síntese química , Antibacterianos/química , Antibacterianos/farmacologia , Hemaglutinação/efeitos dos fármacos , Manosídeos/química , Manosídeos/farmacologia , Infecções Urinárias/tratamento farmacológicoRESUMO
The effect of carbon is subtle but sweet: The flexible C-linkage in the newly synthesised C-glycosyl mimetic, Manalpha(1,6)-C-ManalphaOPh allows OH--pi bonding, both in the gas phase and in aqueous solution. This interaction is absent in the O-linked disaccharide (see figure).The intrinsic conformational preference of a newly synthesised glycomimetic, the C-linked disaccharide Manalpha(1,6)-C-ManalphaOPh (1), has been determined in the gas phase at about 10 K by infrared ion dip spectroscopy coupled with density functional theory and ab initio calculations, and compared with its dynamical conformation in aqueous solution at 298 K by NMR spectroscopy. Comparisons are also made between these conformations and those of the corresponding O-linked disaccharide 2 in the gas phase and the C-linked disaccharide Manalpha(1,6)-C-ManalphaOMe (3) in the gas phase and in aqueous solution. The C- and O-linked disaccharides 1 and 2 present quite distinct conformational preferences in the gas phase: inter-glycosidic hydrogen bonding, seen in one of the two conformers populated in 2, is not seen in 1 which adopts a conformation (not populated in 2) with glycosidic dihedral angles (phi, psi, omega) of -72 degrees , 52 degrees and 66 degrees ; supported in part by an OH--pi hydrogen bond. This conformer is also strongly populated in an aqueous solution of 1 (and very weakly, of 3) together with a second conformer, with dihedral angles (phi, psi, omega) of about -60 degrees , 180 degrees and 60 degrees , not seen in the gas phase but by far the dominant conformer in an aqueous solution of 3. The C-disaccharide 1 was tested as a potential inhibitor, but displayed no significant inhibitory activity against Jack Bean alpha-mannosidase.
Assuntos
Dissacarídeos/síntese química , Manosídeos/síntese química , alfa-Manosidase/efeitos dos fármacos , Dissacarídeos/química , Dissacarídeos/farmacologia , Espectroscopia de Ressonância Magnética , Manosídeos/química , Manosídeos/farmacologia , Estrutura MolecularRESUMO
Glycosyl tricyclic orthoesters provide a versatile basis for the efficient generation of glycosyl phosphates, which are used in the automated synthesis of lipomannan backbone alpha(1-6) hexa-mannoside.
Assuntos
Álcoois/química , Glucose/química , Lipopolissacarídeos/química , Oligossacarídeos/síntese química , Fosfatos/química , Automação , Cromatografia Líquida de Alta Pressão , Manosídeos/síntese química , Oligossacarídeos/químicaRESUMO
A series of synthetic mannosides was screened in a cell-free system for their ability to act as acceptor substrates for mycobacterial mannosyltransferases. Evaluation of these compounds demonstrated the incorporation of [14C]Man from GDP-[14C]Man into a radiolabeled organic-soluble fraction and analysis by thin layer chromatography and autoradiography revealed the formation of two radiolabeled products. Each synthetic acceptor was capable of accepting one or two mannose residues, resulting in a major and a minor mannosylated product. Both products from each acceptor were isolated and their mass was confirmed by fast-atom bombardment-mass spectrometry (FABMS). Characterization of each mannosylated product by exo-glycosidase digestion. acetolysis and linkage analysis by gas chromatography mass spectrometry of partially per-O-methylated alditols, revealed only alpha1-6-linked products. In addition. the antibiotic amphomycin selectively inhibited the formation of mannosylated products suggesting polyprenolmonophosphate-mannose (C15 50-P-Man) was the immediate mannose donor in all mannosylation reactions observed. The ability of synthetic disaccharides to act as acceptor substrates in this system, is most likely due to the action of a mycobacterial polyprenol-P-Man:mannan alpha1-6 mannosyltransferase involved in the biosynthesis of linear alpha1-6-linked lipomannan.
Assuntos
Manosídeos/química , Manosiltransferases/metabolismo , Mycobacterium smegmatis/enzimologia , Sequência de Carboidratos , Cromatografia Gasosa-Espectrometria de Massas , Manosídeos/síntese química , Dados de Sequência Molecular , Espectrometria de Massas de Bombardeamento Rápido de ÁtomosRESUMO
The calcium dependent E-selectin/sialyl Lewisx (sLex) interaction plays a key role in inflammation where it mediates the rolling of leukocytes prior to firm adhesion and extravasation from the vasculature. A model of E-selectin/sLex binding, along with previously reported structure-activity relationships of sLex-related oligosaccharide, was used in the rational design of non-oligosaccharide inhibitors of this pivotal interaction. A palladium-mediated biaryl-coupling (Suzuki) reaction was used as the key step to prepare a number of substituted biphenyls which were assayed for their ability to inhibit the binding of E-, P-, and L-selectin-IgG fusion proteins to sLex expressed on the surface of HL60 cells. Some of the compounds developed had greater in vitro potency than the parent sLex tetrasaccharide and are currently being evaluated in in vivo models of inflammation to select a candidate for clinical development.
Assuntos
Anti-Inflamatórios não Esteroides/síntese química , Compostos de Bifenilo/síntese química , Compostos de Bifenilo/farmacologia , Ácidos Carboxílicos/síntese química , Ácidos Carboxílicos/farmacologia , Selectina E/metabolismo , Manosídeos/síntese química , Manosídeos/farmacologia , Animais , Anti-Inflamatórios não Esteroides/química , Anti-Inflamatórios não Esteroides/farmacologia , Sítios de Ligação , Cálcio/química , Cálcio/metabolismo , Ácidos Carboxílicos/química , Gráficos por Computador , Desenho de Fármacos , Células HL-60 , Humanos , Imunoglobulina G/genética , Imunoglobulina G/metabolismo , Antígenos do Grupo Sanguíneo de Lewis/metabolismo , Manosídeos/química , Camundongos , Modelos Moleculares , Oligossacarídeos/metabolismo , Proteínas Recombinantes de Fusão/genética , Proteínas Recombinantes de Fusão/metabolismo , Selectinas/metabolismo , Antígeno Sialil Lewis X , Relação Estrutura-AtividadeRESUMO
Carbocyclic analogues 2 and 3 of the "trimannosyl structure 1", methyl 3,6-bis(5a-carba-alpha-D-mannopyranosyl)-3,6-dideoxy-alpha-D-ma nnopyranosides bonded by way of respective ether and imino linkages, were synthesized. The ether 2 had no inhibitory activity against alpha-D-mannosidase; in contrast, the imino compound 3 was a mild inhibitor. Furthermore, the inhibitory activity of 4, related to 3 by introduction of unsaturation between C-5 and C-5a of the carba-sugar moieties, was shown to be somewhat greater.
Assuntos
Manose/química , Manosídeos/síntese química , Trissacarídeos/síntese química , Configuração de Carboidratos , Sequência de Carboidratos , Éteres/química , Espectroscopia de Ressonância Magnética , Manose/metabolismo , Manosidases/antagonistas & inibidores , Manosidases/metabolismo , Manosídeos/farmacologia , Dados de Sequência Molecular , Estrutura Molecular , Trissacarídeos/farmacologia , alfa-ManosidaseRESUMO
Streptavidin substituted with mannose residues increased by 20-fold the intracellular concentration of a biotinylated dodecakis(alpha-deoxythymidylate) in macrophages by comparison with the uptake of free oligodeoxynucleotide. Streptavidin, the bacterial homologue of the very basic avidin, which does not contain any carbohydrate moieties and is a neutral protein, was substituted with 12 mannose residues in order to be recognized and internalized by mannose-specific lectins on the surface of macrophages. A 3'-biotinylated and 5'-fluoresceinylated dodecakis (alpha-deoxythymidylate) was synthesized and bound onto mannosylated streptavidin. The conjugate was isolated, and by using flow cytometry, it was shown that the uptake of fluoresceinylated oligodeoxynucleotides bound to mannosylated streptavidin by macrophages is 20-fold higher than that of free oligodeoxynucleotides and that the uptake was competively inhibited by mannosylated serum albumin. Glycosylated streptavidin conjugates recognizing specific membrane lectins on different cells provide the possibility to target biotinylated antisense oligodeoxynucleotides and to increase the biological effect of these chemotherapeutic agents.
Assuntos
Proteínas de Bactérias/química , Biotina/análogos & derivados , Macrófagos/metabolismo , Manosídeos/química , Oligodesoxirribonucleotídeos/metabolismo , Timidina Monofosfato/análogos & derivados , Animais , Proteínas de Bactérias/síntese química , Biotina/síntese química , Biotina/metabolismo , Linhagem Celular , Eletroforese em Gel de Poliacrilamida , Endocitose , Citometria de Fluxo , Manosídeos/síntese química , Camundongos , Oligodesoxirribonucleotídeos/síntese química , Estreptavidina , Timidina Monofosfato/síntese química , Timidina Monofosfato/metabolismoRESUMO
A number of novel, aryl and aralkyl D-mannopyranosides and 1-thio-D-mannopyranosides were synthesized for evaluation of insulin-like and insulin-antagonistic properties. The substituted-phenyl alpha-D-mannopyranosides were prepared by the general procedure of Helferich and Schmitz-Hillebrecht, the substituted-phenyl 1-thio-alpha-D-mannopyranosides by a method corresponding to the Michael synthesis of aromatic glycosides, and the aralkyl 1-thio-alpha-D-mannopyranosides by aralkylation of 2,3,4,6-tetra-O-acetyl-1-thio-alpha-D-mannopyranose (15) and subsequent O-deacetylation. Compound 15 was obtained by basic cleavage of the amidino group in 2-S-(tetra-O-acetyl-alpha-D-mannopyranosyl)-2-thiopseudourea hydrobromide, the product of the reaction of tetra-O-acetyl-alpha-D-mannosyl bromide with thiourea. Benzyl 1-thio-beta-D-mannopyranoside, obtained by reaction of the sodium salt of 1-thio-beta-D-mannopyranose with alpha-bromotoluene, and benzyl 1-thio-alpha-L-mannopyranoside were also synthesized, in order to assess the stereospecificity of the biological activities measured.