Assuntos
Protocolos de Quimioterapia Combinada Antineoplásica/uso terapêutico , Linfoma Extranodal de Células T-NK/diagnóstico por imagem , Linfoma Extranodal de Células T-NK/tratamento farmacológico , Neoplasias Maxilares/diagnóstico por imagem , Neoplasias Maxilares/tratamento farmacológico , Neoplasias de Tecidos Moles/diagnóstico por imagem , Neoplasias de Tecidos Moles/tratamento farmacológico , Biomarcadores Tumorais/sangue , Meios de Contraste , DNA de Neoplasias/sangue , Diagnóstico Diferencial , Humanos , Biópsia Guiada por Imagem , Imuno-Histoquímica , Linfoma Extranodal de Células T-NK/virologia , Masculino , Neoplasias Maxilares/virologia , Pessoa de Meia-Idade , Invasividade Neoplásica , Reação em Cadeia da Polimerase , Tomografia por Emissão de Pósitrons combinada à Tomografia Computadorizada , Radiografia Panorâmica , Neoplasias de Tecidos Moles/virologiaRESUMO
Classically, the HIV/AIDS-related lymphomas are of the B cell type and involve the central nervous system and the abdominal cavity. Primary maxillary sinus lymphoma is rare. Plasmablastic lymphoma (PBL) is an aggressive form of non-Hodgkinvs lymphoma, and is extremely rare. Here we present a case of plasmablastic lymphoma with primary site being maxillary sinus, a rare location.
Assuntos
Infecções por HIV/complicações , Linfoma Relacionado a AIDS/patologia , Neoplasias Maxilares/patologia , Seio Maxilar/diagnóstico por imagem , Mucosa Bucal/patologia , Linfoma Plasmablástico/patologia , Tomografia Computadorizada por Raios X/métodos , Adulto , Protocolos de Quimioterapia Combinada Antineoplásica/uso terapêutico , Biópsia , Ciclofosfamida , Doxorrubicina , Infecções por HIV/patologia , HIV-1 , Humanos , Linfoma Relacionado a AIDS/complicações , Linfoma Relacionado a AIDS/tratamento farmacológico , Masculino , Mandíbula/diagnóstico por imagem , Neoplasias Maxilares/virologia , Linfoma Plasmablástico/complicações , Linfoma Plasmablástico/tratamento farmacológico , Prednisona , Radiografia Panorâmica , Resultado do Tratamento , VincristinaRESUMO
The potential role of viruses with oncogenic potential such as human papilloma virus, cytomegalovirus, Epstein-Barr virus, herpes simplex virus type 1 and 2, herpes virus type 6, in the development of benign and pre-cancerous tumors of maxillofacial region was assessed in the study. We examined 26 patients with tumors in maxillofacial region (skin and mucosa) using molecular-genetic and histological studies of surgically removed neoplasms removed. In 53.8% of the examined samples DNA of Epstein-Barr virus, herpes virus type 6, herpes simplex virus type 1, or cytomegalovirus and in 35.7% of them the association of the above mentioned viruses were detected. It may confirm their relation with the development of benign, precancerous and malignant neoplasms in maxillofacial region.
Assuntos
Transformação Celular Viral , Neoplasias Faciais/virologia , Neoplasias Maxilares/virologia , Vírus Oncogênicos/fisiologia , Lesões Pré-Cancerosas/virologia , Neoplasias Cutâneas/virologia , Adolescente , Adulto , Idoso , DNA Viral/isolamento & purificação , Feminino , Humanos , Masculino , Pessoa de Meia-Idade , Vírus Oncogênicos/isolamento & purificação , Adulto JovemRESUMO
PURPOSE: Oral papillary squamous cell carcinoma (OPSCC) is a histologic variant of SCC with a growth pattern suggesting human papillomavirus (HPV) infection. The aim of this study was to investigate the presence of HPV genotypes in OPSCC. MATERIALS AND METHODS: All cases with a histologic diagnosis of OPSCC from 1993 through 2008 were retrieved and confirmed. Immunohistochemical evaluation for the surrogate marker p16(INK4A) and HPV polymerase chain reaction were performed in tissue and DNA derived from formalin-fixed paraffin-embedded tissue. RESULTS: Forty-four patients with confirmed OPSCC (mean age, 71.96 yr; female-to-male ratio, 1.75:1) comprised the study population. The most common site of involvement was the gingiva followed by the palate and buccal mucosa. Forty cases exhibited an invasive component, 1 was noninvasive, and in 3 cases invasion could not be confirmed owing to suboptimal thickness of the biopsy. Paraffin tissue blocks were available in 41 cases. Twenty-three cases (56.1%) exhibited positive p16(INK4A) staining, which was primarily weak to moderate with a generally focal pattern. Polymerase chain reaction assays were negative for HPV DNA in all cases. CONCLUSIONS: In this study, there was a clinical predilection of OPSCC in older women, with most cases occurring in the masticatory mucosa. Weak to moderate and focal p16(INK4A) staining was appreciated in contrast to reported staining properties in genital and oropharyngeal PSCC. Failure of the polymerase chain reaction assay to exhibit transcriptionally active HPV genotypes suggests that HPV is not associated with OPSCC tumorigenesis.
Assuntos
Alphapapillomavirus/isolamento & purificação , Carcinoma de Células Escamosas/virologia , Inibidor p16 de Quinase Dependente de Ciclina/análise , Neoplasias Bucais/virologia , Infecções por Papillomavirus/diagnóstico , Idoso , Alphapapillomavirus/genética , Biomarcadores Tumorais/análise , DNA Viral/análise , Feminino , Genótipo , Neoplasias Gengivais/virologia , Humanos , Imuno-Histoquímica , Masculino , Neoplasias Mandibulares/virologia , Neoplasias Maxilares/virologia , Neoplasias Palatinas/virologia , Reação em Cadeia da PolimeraseAssuntos
Antirreumáticos/efeitos adversos , Linfoma de Células B/induzido quimicamente , Linfoma Difuso de Grandes Células B/induzido quimicamente , Neoplasias Maxilares/induzido quimicamente , Metotrexato/efeitos adversos , Infecções por Vírus Epstein-Barr/complicações , Feminino , Humanos , Linfoma de Células B/complicações , Linfoma de Células B/virologia , Linfoma Difuso de Grandes Células B/complicações , Linfoma Difuso de Grandes Células B/virologia , Neoplasias Maxilares/complicações , Neoplasias Maxilares/virologia , Pessoa de Meia-IdadeRESUMO
Plasmablastic lymphoma is a rare subcategory of non-Hodgkin lymphoma frequently associated with human immunodeficiency virus. It is a large B-cell lymphoma that has a predilection for the oral cavity. Clinically, plasmablastic lymphoma may mislead to a diagnosis of Kaposi's sarcoma. When infected, plasmablastic lymphoma may mimic an odontogenic cellulitis. Epstein-Barr virus and human herpesvirus 8 are very often associated. Awareness of this entity can prevent misdiagnosis with nonlymphoid malignancies, notably Kaposi's sarcoma, because this lesion does not express the conventional B-cell markers. Unfortunately, as for other high-grade lymphomas in patients with acquired immunodeficiency syndrome (AIDS), the prognosis is poor. The case of a heterosexual 42-year-old man referred for a right hemifacial neoplasm is reported.
Assuntos
HIV-1 , Linfoma Relacionado a AIDS/patologia , Neoplasias Maxilares/patologia , Adulto , Humanos , Linfoma Relacionado a AIDS/virologia , Masculino , Neoplasias Maxilares/virologiaRESUMO
Using PCR, type A Epstein-Barr virus (EBV) infection was demonstrated in a squamous cell carcinoma of the maxilla (in a 52-year-old man) and the tongue of the same patient 18 years later (at the age of 70). Furthermore, at the age of 72, this patient developed an EBV-infected anaplastic large cell lymphoma. Analysis of the terminal regions of the EBV genome revealed a monoclonal proliferation of EBV-infected lymphoma cells. However, sequence analysis of the EBV revealed a slight difference in the EBNA2 regions between the virus-infected lymphoma and the squamous cell carcinomas. The mutations at 48991 (G-->T) and 48998 (C-->A) were demonstrated in the lymphoma. Although the squamous cell carcinoma of the tongue occurred after an interval of 18 years, the mutation site in the carcinomas was the same, 49137 (A-->G), as compared with B95-8 strain EBV EBNA2. The mutations at 48991 and at 49137 were associated with amino acid changes, Arg-->Met and Thr-->Ala, respectively, but the alteration at 48998 was a silent mutation. Thirty-bp deletion in the LMP-1 carboxy terminal region was demonstrated in the virus-infected lymphoma, but not in the squamous cell carcinomas. On the other hand, HTLV-1 proviral DNA (tax, gag and env) was not detected in the lymphoma, nor was HPV demonstrated in the squamous cell carcinomas, although Okinawa is known as an HTLV-1 and HPV prevalence region. The T-cell receptor beta gene rearrangement was demonstrated in the lymphoma, but the t(2;5) fusion transcript was not detected using PCR. Cytogenetic analysis of the lymphoma cells showed a complex hypertriploid karyotype with 76XY. The type A EBV infection might play a role in the carcinogenesis of the tumors of our patient. Interestingly, the infected virus genome sequences, the EBNA2 and LMP-1 regions, which were closely associated with carcinogenesis in the squamous cell carcinomas and the lymphoma, showed slight differences.
Assuntos
Carcinoma de Células Escamosas/virologia , Infecções por Vírus Epstein-Barr/virologia , Antígenos Nucleares do Vírus Epstein-Barr/genética , Herpesvirus Humano 4/genética , Linfoma Anaplásico de Células Grandes/virologia , Neoplasias Maxilares/virologia , Neoplasias Primárias Múltiplas/virologia , Neoplasias da Língua/virologia , Proteínas da Matriz Viral/genética , Idoso , Sequência de Bases , Carcinoma de Células Escamosas/metabolismo , Carcinoma de Células Escamosas/patologia , Bandeamento Cromossômico , DNA de Neoplasias/análise , Infecções por Vírus Epstein-Barr/metabolismo , Infecções por Vírus Epstein-Barr/patologia , Antígenos Nucleares do Vírus Epstein-Barr/metabolismo , Herpesvirus Humano 4/isolamento & purificação , Humanos , Hibridização In Situ , Cariotipagem , Linfoma Anaplásico de Células Grandes/metabolismo , Linfoma Anaplásico de Células Grandes/patologia , Masculino , Neoplasias Maxilares/metabolismo , Neoplasias Maxilares/patologia , Dados de Sequência Molecular , Neoplasias Primárias Múltiplas/metabolismo , Neoplasias Primárias Múltiplas/patologia , Mutação Puntual , Reação em Cadeia da Polimerase , Neoplasias da Língua/metabolismo , Neoplasias da Língua/patologia , Proteínas da Matriz Viral/metabolismo , Proteínas ViraisRESUMO
A high incidence of a T cell phenotype of sinonasal lymphomas in other Asian countries has been associated with a high incidence of Epstein Barr virus (EBV) infection. We analyzed 13 sinonasal and 18 Waldeyer's ring lymphomas for the prevalence of EBV encoded RNA (EBER) using a sensitive and specific in situ hybridization. In addition, we examined the relationship of histologic findings and immunophenotype as well as the location of the lymphomas to the presence of EBV. The EBER was detected in each of 12 sinonasal lymphomas with a T cell immunophenotype. One B cell sinonasal lymphoma was EBER negative. Four of 18 Waldeyer's ring lymphomas were positive for EBER, including two T cell lymphomas. Two of 16 B cell Waldeyer's ring lymphomas were EBER positive. Morphologically, 11 of 20 diffuse large cell lymphomas, 2 diffuse mixed small and large cell lymphomas, 2 of 4 immunoblastic lymphomas and 1 lymphoplasmacytic lymphoma were EBER positive. Four follicular large cell lymphomas were EBER negative. A characteristic angiocentric or angiodestructive pattern was found in most T cell lymphomas and EBER positive cases. These findings indicate that EBV infection is more strongly associated with the T cell immunophenotype, angiocentric pattern and sinonasal location of the lymphoma.