Assuntos
Células Epiteliais/imunologia , Imunidade/imunologia , Mucosa Intestinal/imunologia , NF-kappa B/imunologia , Animais , Citocinas/imunologia , Citocinas/metabolismo , Sistema Nervoso Entérico/citologia , Sistema Nervoso Entérico/imunologia , Sistema Nervoso Entérico/metabolismo , Células Epiteliais/metabolismo , Humanos , Mucosa Intestinal/citologia , Mucosa Intestinal/metabolismo , NF-kappa B/metabolismo , Neurônios/imunologia , Neurônios/metabolismo , Neurotransmissores/imunologia , Neurotransmissores/metabolismo , Células Estromais/citologia , Células Estromais/imunologia , Células Estromais/metabolismoRESUMO
T cells are essential for eradicating microorganisms and cancer and for tissue repair, have a pro-cognitive role in the brain, and limit Central Nervous System (CNS) inflammation and damage upon injury and infection. However, in aging, chronic infections, acute SARS-CoV-2 infection, cancer, chronic stress, depression and major injury/trauma, T cells are often scarce, exhausted, senescent, impaired/biased and dysfunctional. People with impaired/dysfunctional T cells are at high risk of infections, cancer, other diseases, and eventually mortality, and become multi-level burden on other people, organizations and societies. It is suggested that "Nerve-Driven Immunity" and "Personalized Adoptive Neuro-Immunotherapy" may overcome this problem. Natural Neurotransmitters and Neuropeptides: Glutamate, Dopamine, GnRH-II, CGRP, Neuropeptide Y, Somatostatin and others, bind their well-characterized receptors expressed on the cell surface of naïve/resting T cells and induce multiple direct, beneficial, and therapeutically relevant effects. These Neurotransmitters and Neuropeptides can induce/increase: gene expression, cytokine secretion, integrin-mediated adhesion, chemotactic migration, extravasation, proliferation, and killing of cancer. Moreover, we recently found that some of these Neurotransmitters and Neuropeptides also induce rapid and profound decrease of PD-1 in human T cells. By inducing these beneficial effects in naïve/resting T cells at different times after binding their receptors (i.e. NOT by single effect/mechanism/pathway), these Neurotransmitters and Neuropeptides by themselves can activate, rejuvenate, and improve T cells. "Personalized Adaptive Neuro-Immunotherapy" is a novel method for rejuvenating and improving T cells safely and potently by Neurotransmitters and Neuropeptides, consisting of personalized diagnostic and therapeutic protocols. The patient's scarce and/or dysfunctional T cells are activated ex vivo once by pre-selected Neurotransmitters and/or Neuropeptides, tested, and re-inoculated to the patient's body. Neuro-Immunotherapy can be actionable and repeated whenever needed, and allows other treatments. This adoptive Neuro-Immunotherapy calls for testing its safety and efficacy in clinical trials.
Assuntos
Encéfalo/imunologia , COVID-19/imunologia , Neuropeptídeos/imunologia , Neurotransmissores/imunologia , SARS-CoV-2/imunologia , Linfócitos T/imunologia , Encéfalo/patologia , Encéfalo/virologia , COVID-19/patologia , Humanos , Rejuvenescimento , Linfócitos T/patologiaRESUMO
Immune cells rely on cell-cell communication to specify and fine-tune their responses. They express an extensive network of cell communication modes, including a vast repertoire of cell surface and transmembrane receptors and ligands, membrane vesicles, junctions, ligand and voltage-gated ion channels, and transporters. During a crosstalk between the nervous system and the immune system these modes of cellular communication and the downstream signal transduction events are influenced by neurotransmitters present in the local tissue environments in an autocrine or paracrine fashion. Neurotransmitters thus influence innate and adaptive immune responses. In addition, immune cells send signals to the brain through cytokines, and are present in the brain to influence neural responses. Altered communication between the nervous and immune systems is emerging as a common feature in neurodegenerative and immunopathological diseases. Here, we present the mechanistic frameworks of immunostimulatory and immunosuppressive effects critical neurotransmitters - dopamine (3,4-dihydroxyphenethylamine), serotonin (5-hydroxytryptamine), substance P (trifluoroacetate salt powder), and L-glutamate - exert on lymphocytes and non-lymphoid immune cells. Furthermore, we discuss the possible roles neurotransmitter-driven neuroimmune networks play in the pathogenesis of neurodegenerative disorders, autoimmune diseases, cancer, and outline potential clinical implications of balancing neuroimmune crosstalk by therapeutic modulation.
Assuntos
Neuroimunomodulação/imunologia , Neurotransmissores/imunologia , Animais , HumanosRESUMO
Despite the major progresses in comprehending the mechanisms of tumor immunosurveillance and the role of innate and adaptive immune systems in recent years, there are still a number of obstacles hindering successful and effective immunotherapy of cancer. Such obstacles have been mainly attributed to the ability of tumors in creating a tolerant microenvironment and exploiting a plethora of immunosuppressive factors that counter effective immune responses against tumor cells. Here we represent a new insight into probable links between immune system disability with metabolism and chronic psychological stress which is beyond the other strategies recruited by tumors to thwart tumor immunosurveillance. In addition, we underscore the prominent role of improper innate immune responses as one of the underlying causes of either pro-tumorigenic capability or tumor immunosurveillance failure. However the insufficiency of stimulatory factors in immune responses is a major fact leading to tumor survival, metabolic suppression of immune cells in tumor microenvironment, as well as the negative influences of chronic stress and depression in cancer patients are important parameters amplifying disability of immune responses which have mostly been underestimated in cancer immunotherapy. Stress-related catecholamines are suggested as immunosuppressive factors. In addition, tumor cells have distinct metabolic pathways and secrete various metabolites in the tumor microenvironment which may inhibit T cells activity. We believe that simultaneous control of metabolic and psychological negative influences on the tumor immunosurveillance, along with addressing the weak aspects of innate and adaptive immune responses in cancer immunotherapy may result in more successful treatment of tumors.
Assuntos
Imunidade Inata , Imunoterapia/métodos , Neoplasias/imunologia , Neurotransmissores/imunologia , Estresse Psicológico/imunologia , Animais , Carcinogênese/imunologia , Modelos Animais de Doenças , Humanos , Vigilância Imunológica , Neoplasias/metabolismo , Neoplasias/psicologia , Neoplasias/terapia , Neurotransmissores/metabolismo , Estresse Psicológico/metabolismo , Estresse Psicológico/psicologia , Evasão Tumoral , Microambiente Tumoral/imunologiaRESUMO
Cognitive dysfunction is one of the most common postoperative complications experienced by older patients after anesthesia and surgery but the cause remains unknown. Immune molecules are essential for many aspects of neural homeostasis, including learning and memory, and an imbalance in immune neuromodulators is implicated in the development of neural dysfunction. Aging alters the control of neuroinflammatory cascades and general anesthetics are immunosuppressants. Therefore, we hypothesized that general anesthesia disturbs neuroimmune signaling in an age-dependent fashion. We tested this hypothesis by examining gene expression of key immune neuromodulators including IL-1ß, TNFα, and CCL2 in the hippocampus of young adult (3 mo) and aged (20 mo) mice following isoflurane anesthesia. We show that isoflurane anesthesia increases expression of these signaling molecules in the hippocampus of young adult mice but decreases it in the hippocampus of old mice. Furthermore, anesthetized old mice had an amplified hippocampal neuroimmune response to systemically administered lipopolysaccharide compared to age-matched carrier controls. Together, these data indicate that isoflurane anesthesia disrupts hippocampal neuroimmune mediator gene expression in the old brain and suggests a potential mechanism by which general anesthesia can contribute to disordered neuronal homeostasis and post-anesthesia cognitive disability in older subjects.
Assuntos
Envelhecimento/imunologia , Anestésicos Inalatórios/efeitos adversos , Hipocampo/efeitos dos fármacos , Hipocampo/imunologia , Isoflurano/efeitos adversos , Neurotransmissores/genética , Neurotransmissores/imunologia , Idoso , Envelhecimento/genética , Envelhecimento/psicologia , Animais , Quimiocina CCL2/genética , Disfunção Cognitiva/etiologia , Expressão Gênica/efeitos dos fármacos , Humanos , Fatores Imunológicos/genética , Interleucina-10/genética , Interleucina-1beta/genética , Lipopolissacarídeos/administração & dosagem , Masculino , Camundongos , Camundongos Endogâmicos C57BL , Modelos Animais , Complicações Pós-Operatórias/etiologia , Fator de Necrose Tumoral alfa/genéticaRESUMO
The neuroimmunoregulation of inflammation has been well characterized. Entamoeba histolytica provokes an inflammatory response in the host in which macrophages and neutrophils are the first line of defense. The aim of this study was to analyze the effect of the 220 kDa lectin of Entamoeba histolytica on stimulation of human macrophages and neutrophils, especially the secretion of cytokines and the relation of these to neurotransmitters. Human cells were interacted with L220, epinephrine, nicotine, esmolol and vecuronium bromide. The concentrations of IL-1ß, IFN-γ, TNF-α and IL-10 were determined by ELISA at, 4 h of interaction. L220 has a cytokine stimulating function of macrophages and neutrophils for secretion of IL-1ß, and IL-10 only by macrophages, which was modulated by the effect of vecuronium on cholinergic receptors in this immune cells.
Assuntos
Entamoeba histolytica/imunologia , Ativação de Macrófagos/efeitos dos fármacos , Macrófagos/efeitos dos fármacos , Neurotransmissores/farmacologia , Ativação de Neutrófilo/efeitos dos fármacos , Neutrófilos/efeitos dos fármacos , Proteínas de Protozoários/farmacologia , Adolescente , Citocinas/efeitos dos fármacos , Citocinas/imunologia , Entamoeba histolytica/química , Epinefrina/farmacologia , Humanos , Lectinas/química , Lectinas/imunologia , Lectinas/farmacologia , Macrófagos/microbiologia , Macrófagos/fisiologia , Neurotransmissores/imunologia , Neutrófilos/microbiologia , Neutrófilos/fisiologia , Nicotina/farmacologia , Proteínas de Protozoários/química , Proteínas de Protozoários/imunologia , Brometo de Vecurônio/farmacologia , Adulto JovemRESUMO
Group 3 innate lymphoid cells (ILC3) are major regulators of inflammation and infection at mucosal barriers. ILC3 development is thought to be programmed, but how ILC3 perceive, integrate and respond to local environmental signals remains unclear. Here we show that ILC3 in mice sense their environment and control gut defence as part of a glialILC3epithelial cell unit orchestrated by neurotrophic factors. We found that enteric ILC3 express the neuroregulatory receptor RET. ILC3-autonomous Ret ablation led to decreased innate interleukin-22 (IL-22), impaired epithelial reactivity, dysbiosis and increased susceptibility to bowel inflammation and infection. Neurotrophic factors directly controlled innate Il22 downstream of the p38 MAPK/ERK-AKT cascade and STAT3 activation. Notably, ILC3 were adjacent to neurotrophic-factor-expressing glial cells that exhibited stellate-shaped projections into ILC3 aggregates. Glial cells sensed microenvironmental cues in a MYD88-dependent manner to control neurotrophic factors and innate IL-22. Accordingly, glial-intrinsic Myd88 deletion led to impaired production of ILC3-derived IL-22 and a pronounced propensity towards gut inflammation and infection. Our work sheds light on a novel multi-tissue defence unit, revealing that glial cells are central hubs of neuron and innate immune regulation by neurotrophic factor signals.
Assuntos
Imunidade Inata , Intestinos/imunologia , Linfócitos/imunologia , Neuroglia/metabolismo , Neurotransmissores/metabolismo , Animais , Microambiente Celular/imunologia , Células Epiteliais/citologia , Células Epiteliais/imunologia , Feminino , Microbioma Gastrointestinal/imunologia , Imunidade nas Mucosas , Inflamação/imunologia , Inflamação/metabolismo , Interleucinas/biossíntese , Interleucinas/imunologia , Mucosa Intestinal/citologia , Mucosa Intestinal/imunologia , Intestinos/citologia , Linfócitos/citologia , Linfócitos/metabolismo , Sistema de Sinalização das MAP Quinases , Masculino , Camundongos , Fator 88 de Diferenciação Mieloide/deficiência , Fator 88 de Diferenciação Mieloide/metabolismo , Neuroglia/imunologia , Neurotransmissores/imunologia , Proteínas Proto-Oncogênicas c-akt/metabolismo , Proteínas Proto-Oncogênicas c-ret/deficiência , Proteínas Proto-Oncogênicas c-ret/metabolismo , Fator de Transcrição STAT3/metabolismo , Interleucina 22RESUMO
All organs of the immune system are innervated and almost all neurotransmitter receptors are present on immune cells. We studied the effects of sympathetic innervation in the development of amebic liver abscess (ALA) in rats. Our results showed that lack of sympathetic innervation promote a decrease in size of ALA. We found scarce amoebas, increased the number of neutrophils and a few collagen fibers surrounding the abscess, meanwhile in control group, we observed abscesses areas with typical necrosis including trophozoites and neutrophils. Macrophages were differentially distributed surrounding abscess area in control and vehicle groups, but equally located in and outside of the abscesses in sympathectomized rat. No significant differences were observed on NK cells in analysed groups. In cytokines quantification studies, we observed down-expression of IFN-g and TNF-a, moreover, we found overexpression of IL-10 in sympathectomized and ALA group. In conclusion, our results suggest that elimination of sympathetic nerve fibers in a model rat of amebic liver abscess induces reduction of the innate immune response and presence of amebas through the liver at seven days post-inoculation.
Todos los órganos del sistema inmune están inervados y casi todos los receptores para neurotransmisores están presentes en las células de la respuesta inmune. Nosotros estudiamos el efecto de la inervación simpática en el desarrollo del Absceso Hepático Amebiano (AHA) en ratas. Nuestros resultados muestran que la inervación simpática promueve una disminución en el tamaño del AHA. Nosotros encontramos áreas fibróticas bien definidas con algunas amibas, mayor número de neutrófilos y pocas fibras de colágena rodeando el área de daño, mientras que en el grupo control, nosotros observamos áreas con necrosis, trofozoítos y pocos neutrófilos en el área fibrótica. Los macrófagos se observaron distribuidos en el área fibrótica en los animales simpatectomizados, mientras que en los controles encontramos a los macrófagos distribuidos en la periferia del absceso. No se encontró diferencia significativa en la distribución y cantidad de células NK. En el estudio de citocinas nosotros observamos una disminución de IFN-g y TNF-a y un incremento de IL-10 en animales simpatectomizados. En conclusión, nuestros resultados sugieren que la eliminación de las fibras del sistema nervioso simpático en el modelo de AHA en rata, reduce la respuesta inmune innata y persisten amebas en el tejido dañados a los 7 días post-inoculación.
Assuntos
Animais , Masculino , Ratos , Abscesso Hepático Amebiano/imunologia , Sistema Nervoso Simpático/imunologia , Sistema Nervoso Simpático/metabolismo , Entamoeba histolytica , Imunidade Inata , Imuno-Histoquímica , Abscesso Hepático Amebiano/metabolismo , Microscopia Eletrônica de Transmissão , Neurotransmissores/imunologia , Ratos Wistar , Simpatectomia QuímicaRESUMO
Neuro-immune interaction in the gut is substantially involved in the maintenance of intestinal immune homeostasis and the pathology of intestinal immune diseases. We have previously demonstrated that mucosal mast cells and nerve fibers containing CGRP, a neurotransmitter of intrinsic enteric sensory neurons, are markedly increased and exist in close proximity to each other in the colon of food allergy (FA) mice. In the present study, a CGRP-receptor antagonist BIBN4096BS significantly alleviated allergic symptoms in the murine FA model. In addition, the elevated numbers of mucosal mast cells in the proximal colon of FA mice were significantly decreased in that of BIBN4096BS-treated FA mice. Thus, we investigated the effects of CGRP on calcium-independent process in degranulation of mucosal mast cells since CGRP increases intracellular cAMP levels, but not Ca(2+) concentration. CGRP did not alter a calcium ionophore A23187-increased cytosolic Ca(2+) concentration in mucosal-type bone marrow-derived mast cells (mBMMCs), but did augment microtubule reorganization in resting and A23187-activated mBMMCs. Furthermore, CGRP alone failed to cause the degranulation of mBMMCs, but CGRP significantly enhanced the degranulation of mBMMCs induced by A23187. Together, these data indicate that CGRP- enhanced microtubule reorganization augments IgE-independent/non-antigenic stimuli-induced mucosal mast cell degranulation, thereby contributing to the development of FA.
Assuntos
Peptídeo Relacionado com Gene de Calcitonina/imunologia , Hipersensibilidade Alimentar/imunologia , Mastócitos/metabolismo , Microtúbulos/metabolismo , Neurotransmissores/imunologia , Células Receptoras Sensoriais/imunologia , Animais , Antagonistas do Receptor do Peptídeo Relacionado ao Gene de Calcitonina , Diarreia/tratamento farmacológico , Modelos Animais de Doenças , Hipersensibilidade Alimentar/terapia , Masculino , Mastócitos/imunologia , Mastocitose/tratamento farmacológico , Camundongos , Camundongos Endogâmicos BALB C , Ovalbumina/efeitos adversos , Piperazinas/farmacologia , Quinazolinas/farmacologiaRESUMO
Up to now, the 'hardwired' neural pathway of the neuro-immune regulation is not fully understood. Here we reported a new neural pathway which links sympathetic nerves with immune cells of the lymphoid tissues. Our results demonstrated that nerve fibers derived from superior cervical ganglion directly targeted only S100(+) cells in the cervical lymph nodes. Moreover, we found co-expression of neurotransmitters such as norepinephrine, vasoactive intestinal polypeptide and neuropeptide Y in the postganglionic sympathetic nerve endings that innervate S100(+) cells. Our findings suggested that S100(+) cells serve as a neuro-immune cross-talker in lymph organs that may play a significant role in transmitting signals of nervous cells to targeted immune cells. The new findings provide better understanding of the cross-talk mechanism between the nervous system and the immune system.
Assuntos
Sistema Imunitário/imunologia , Sistema Imunitário/inervação , Linfonodos/inervação , Sistema Linfático/imunologia , Sistema Linfático/inervação , Fibras Nervosas/imunologia , Animais , Sistema Imunitário/metabolismo , Linfonodos/imunologia , Linfonodos/metabolismo , Sistema Linfático/metabolismo , Masculino , Terminações Nervosas/imunologia , Terminações Nervosas/metabolismo , Fibras Nervosas/metabolismo , Neurotransmissores/imunologia , Neurotransmissores/metabolismo , Ratos , Ratos Sprague-Dawley , Sistema Nervoso Simpático/imunologia , Sistema Nervoso Simpático/metabolismoRESUMO
A sustained neuroinflammatory response is the hallmark of many neurodegenerative diseases, including Parkinson's disease, Alzheimer's disease, amyotrophic lateral sclerosis, multiple sclerosis, and HIV-associated neurodegeneration. A specific subset of T cells, currently recognized as FOXP3(+) CD25(+) CD4(+) regulatory T cells (Tregs), are pivotal in suppressing autoimmunity and maintaining immune homeostasis by mediating self-tolerance at the periphery as shown in autoimmune diseases and cancers. A growing body of evidence shows that Tregs are not only important for maintaining immune balance at the periphery but also contribute to self-tolerance and immune privilege in the central nervous system. In this article, we first review the current status of knowledge concerning the development and the suppressive function of Tregs. We then discuss the evidence supporting a dysfunction of Tregs in several neurodegenerative diseases. Interestingly, a dysfunction of Tregs is mainly observed in the early stages of several neurodegenerative diseases, but not in their chronic stages, pointing to a causative role of inflammation in the pathogenesis of neurodegenerative diseases. Furthermore, we provide an overview of a number of molecules, such as hormones, neuropeptides, neurotransmitters, or ion channels, that affect the dysfunction of Tregs in neurodegenerative diseases. We also emphasize the effects of the intestinal microbiome on the induction and function of Tregs and the need to study the crosstalk between the enteric nervous system and Tregs in neurodegenerative diseases. Finally, we point out the need for a systems biology approach in the analysis of the enormous complexity regulating the function of Tregs and their potential role in neurodegenerative diseases.
Assuntos
Doenças Neurodegenerativas/imunologia , Linfócitos T Reguladores/imunologia , Animais , Pesquisa Biomédica , Citocinas/imunologia , Humanos , Camundongos , Neurotransmissores/imunologia , Biologia de SistemasRESUMO
Macrophages are key players not only during initiation of inflammation but also during its resolution. This is achieved by their high functional plasticity and the demand to recognize an enormous repertoire of danger signals and cytokines/chemokines derived from adaptive immune cells. Studies predominantly conducted over the last two decades implicate that macrophage responses are also modulated by neuronal mediators such as neurotransmitters or neurotrophic factors. Here we summarize the current understanding of neuromediator-dependent interplay between macrophages and the nervous system.
Assuntos
Macrófagos/fisiologia , Neuroimunomodulação , Neurônios/imunologia , Neurotransmissores/imunologia , Animais , Humanos , Inflamação , Regeneração Nervosa/imunologia , Neurotransmissores/metabolismoRESUMO
Dendritic cells (DCs) are responsible of priming T cells and promoting their differentiation from naïve T cells into appropriate effector cells. Each different phenotype of effector T cells promotes the elimination of a determined kind of pathogen or tumour. Thus, DCs and T cells play critical roles on orchestrating adaptive immune responses against specific threats. Because of their fundamental functions at controlling immunity, DCs and T cells require tight regulatory mechanisms to ensure efficient, but safe, immune responses. Several studies have shown that neurotransmitters, in addition to mediate interactions into the nervous system, can contribute to the modulation of immunity by promoting the communication between nervous and immune systems and in the interaction between different immune cells. Due to the pivotal role that the DC-T cell interaction plays in the development and regulation of adaptive immune responses, it is important to understand how the function of these cells may be regulated by neurotransmitters. Here, we review the emerging role of neurotransmitters as regulators of DC and T cell physiology and also how these molecules, by acting on the DC-T cell interaction, may modulate the fate of T cells and, therefore, the nature of the adaptive immune response. Moreover, we discuss how alterations on the neurotransmitter-mediated immune regulatory mechanisms can contribute to the onset of immune-related disorders. In addition, we discuss potential new targets for the design of strategies for therapies against tumours, autoimmunity and neuro-immune related diseases.
Assuntos
Células Dendríticas/imunologia , Ativação Linfocitária/imunologia , Neurotransmissores/imunologia , Linfócitos T/imunologia , Acetilcolina/metabolismo , Imunidade Adaptativa/fisiologia , Apresentação de Antígeno , Dopamina/metabolismo , Ácido Glutâmico/metabolismo , Doenças do Sistema Imunitário/imunologia , Doenças do Sistema Imunitário/fisiopatologia , Imunidade Inata/fisiologia , Sinapses Imunológicas/imunologia , Ligantes , Receptores de Antígenos de Linfócitos T/metabolismo , Serotonina/metabolismo , Subpopulações de Linfócitos T/imunologiaRESUMO
Fatigue is a psychophysiological state that acts on the subject's motivation to engage in and/or to continue strenuous physical or cognitive activities. In various pathological conditions fatigue seems to lose this homeostatic function and presents itself as a symptom. The pathophysiology of fatigue is complex and includes neurological (central and peripheral) dysfunction, and altered neurotransmitter, cytokine, and hormonal settings. Treatment interventions are generally based on a sequential approach including treatment of comorbid factors, nonpharmacological treatments, and drugs.
Assuntos
Sistema Nervoso Central/fisiopatologia , Síndrome de Fadiga Crônica/fisiopatologia , Síndrome de Fadiga Crônica/terapia , Homeostase/imunologia , Sistema Nervoso Central/imunologia , Estimulantes do Sistema Nervoso Central/uso terapêutico , Terapia Cognitivo-Comportamental/normas , Citocinas/imunologia , Citocinas/metabolismo , Diagnóstico Diferencial , Terapia por Exercício/normas , Síndrome de Fadiga Crônica/imunologia , Humanos , Neurotransmissores/imunologia , Neurotransmissores/metabolismoRESUMO
Antiphospholipid antibodies (aPL) generated in experimental animals cross-react with ATP. We therefore examined the possibility that aPL IgG from human subjects bind to ATP by affinity column and an enzyme linked immunosorbent assay (ELISA). Sera with high levels of aPL IgG were collected from 12 patients with the antiphospholipid syndrome (APS). IgG fractions from 10 of 12 APS patients contained aPL that could be affinity-bound to an ATP column and completely eluted with NaCl 0.5 M. A significant (> 50%) inhibition of aPL IgG binding by ATP 5 mM was found in the majority. Similar inhibition was obtained with ADP but not with AMP or cAMP. All the affinity purified anti-ATP antibodies also bound beta2-glycoprotein-I (beta2-GPI, also known as apolipoprotein H) suggesting that, similar to most pathogenic aPL, their binding depends on this serum cofactor. We further investigated this possibility and found that the binding of beta2-GPI to the ATP column was similar to that of aPL IgG in that most was reversed by NaCl 0.5 M. Furthermore, addition of beta2-GPI to aPL IgG significantly increased the amount of aPL binding to an ATP column. We conclude that aPL IgG bind ATP, probably through beta32-GPI. This binding could interfere with the normal extracellular function of ATP and similar neurotransmitters.
Assuntos
Trifosfato de Adenosina/imunologia , Trifosfato de Adenosina/metabolismo , Anticorpos Antifosfolipídeos/metabolismo , Síndrome Antifosfolipídica/complicações , Síndrome Antifosfolipídica/imunologia , Doenças do Sistema Nervoso Central/etiologia , Doenças do Sistema Nervoso Central/imunologia , Adulto , Idoso , Animais , Especificidade de Anticorpos , Síndrome Antifosfolipídica/metabolismo , Ligação Competitiva , Cardiolipinas/imunologia , Cardiolipinas/metabolismo , Doenças do Sistema Nervoso Central/metabolismo , Reações Cruzadas , Feminino , Glicoproteínas/imunologia , Glicoproteínas/metabolismo , Humanos , Imunoglobulina G/metabolismo , Técnicas In Vitro , Pessoa de Meia-Idade , Neurotransmissores/imunologia , Neurotransmissores/metabolismo , Gravidez , beta 2-Glicoproteína IRESUMO
Vasoactive neuropeptides such as pituitary adenylate cyclase activating polypeptide (PACAP), calcitonin gene related peptide (CGRP) and vasoactive intestinal peptide (VIP) have been implicated in a number of fatigue-related conditions. Associations of these vasoactive neuropeptides with heat shock proteins (hsps) and cytosine-guanosine dinucleotide (CpG) DNA fragments in autoimmune phenomena have been postulated to interfere with receptor signal activation for adenylate cyclase and other vital cellular processes. However, a specific mechanism for receptor dysfunction has not been explored to date. G protein-coupled receptors (GPCRs) constitute a high proportion of biological receptor mechanisms and serve a wide range of substances including nucleosides, nucleotides, catecholamines, calcium, histamine, serotonin and prostaglandins. They are complex transmembrane hepta-helical serpentine structures with specific binding capabilities resulting in conformational changes that activate cognate cyclic GMP (G proteins). GPCRs adapt to certain stimuli through desensitisation and changes in phosphorylation and are subject to distortions of signalling processes. Hence, these vital signalling structures are susceptible to impairment of function through a range of mechanisms. One of their vital functions is signalling through adenylate cyclase, a vital step in cyclic AMP metabolism. This step involves ATP metabolism and therefore is a crucial mediator of cellular energy pathways. Some GPCRs act to inhibit adenylate cyclase (Gi proteins). Also vasoactive neuropeptides, such as PACAP display a number of receptor isotypes including null variants. Overexpression of Gi proteins and null variant receptors may account for major disruptions of signal transduction and ATP/cAMP metabolism. This paper examines the possible role of GPCR dysfunction in contributing to fatigue-related vasoactive neuropeptide autoimmune disorders which may include chronic fatigue syndrome (CFS), Gulf War syndrome (GWS) and even sudden infant death syndrome (SIDS).
Assuntos
Doenças Autoimunes/imunologia , Síndrome de Fadiga Crônica/imunologia , Modelos Biológicos , Neuropeptídeos/imunologia , Receptores Acoplados a Proteínas G/metabolismo , Trifosfato de Adenosina/metabolismo , Adenilil Ciclases/metabolismo , Peptídeo Relacionado com Gene de Calcitonina/imunologia , AMP Cíclico/metabolismo , Variação Genética , Humanos , Lactente , Fatores de Crescimento Neural/imunologia , Neurotransmissores/imunologia , Oligopeptídeos/química , Síndrome do Golfo Pérsico/imunologia , Polipeptídeo Hipofisário Ativador de Adenilato Ciclase , Conformação Proteica , Estrutura Secundária de Proteína , Receptores Acoplados a Proteínas G/química , Receptores Acoplados a Proteínas G/genética , Morte Súbita do Lactente/imunologia , Peptídeo Intestinal Vasoativo/imunologiaRESUMO
By using immunohistochemistry, a network of nerve fibers containing pituitary adenylate-cyclase activating polypeptide (PACAP) was demonstrated in the pineal gland of the golden hamster, a photoperiodic species often used in pineal and circadian rhythm research. The nerve fibers are present in the capsule from where they permeate into the pineal perivascular spaces and parenchyma. Immuno-electron microscopy showed the PACAPergic nerve terminals, with clear transmitter vesicles, to terminate in the interstitial spaces between the pinealocytes or in the perivascular spaces. Some of the PACAPergic nerve terminals made synapse-like contacts with the pinealocytes. The origin of the PACAP-containing nerve fibers innervating the pineal gland of the hamster was investigated by combined retrograde tracing with fluorogold and immunohistochemistry for PACAP. A 2% fluorogold solution was injected iontophoretically into the superficial pineal gland and the animals were allowed to survive for 1 wk. After perfusion fixation of the rats, the location of the tracer was investigated in the brain, the parasympathetic sphenopalatine, and otic ganglia, as well as in the sensory trigeminal ganglia. The tracer was found in perikarya of all the investigated ganglia. However, co-localization with PACAP was found only in the trigeminal ganglion.
Assuntos
Fibras Nervosas/imunologia , Fatores de Crescimento Neural/imunologia , Neuropeptídeos/imunologia , Neurotransmissores/imunologia , Glândula Pineal/inervação , Gânglio Trigeminal/imunologia , Animais , Cricetinae , Masculino , Mesocricetus , Microscopia Eletrônica , Microscopia de Fluorescência , Fibras Nervosas/metabolismo , Fibras Nervosas/ultraestrutura , Fatores de Crescimento Neural/metabolismo , Neuropeptídeos/metabolismo , Neurotransmissores/metabolismo , Glândula Pineal/imunologia , Glândula Pineal/metabolismo , Glândula Pineal/ultraestrutura , Polipeptídeo Hipofisário Ativador de Adenilato Ciclase , Gânglio Trigeminal/metabolismoRESUMO
Infections caused by Gram-negative bacteria constitute one of the major causes of septic shock, which results from the inability of the immune system to limit bacterial spread during the ongoing infection. In the last decade, it has been demonstrated that vasoactive intestinal peptide (VIP) and pituitary adenylate cyclase-activating polypeptide (PACAP) are two endogenous immunopeptides, which together with three G protein-coupled receptors (VPAC1, VPAC2, and PAC1) exert a significant, therapeutic effect attenuating the deleterious consequences of septic shock by balancing pro- and anti-inflammatory factors. We have recently shown PAC1 receptor involvement in vivo as an anti-inflammatory receptor, at least in part, by attenuating lipopolysaccharide-induced production of proinflammatory interleukin-6. The present study deepens in the protective role of PAC1 receptor in septic shock, elucidating its involvement in the modulation of neutrophil recruitment and in the expression of different molecular sensors such as intercellular adhesion molecule-1, vascular cell adhesion molecule-1, fibrinogen, serum amyloid A, and nitric oxide as important, systemic players of the development of septic shock. Our results, using a mice deficient in PAC1 and a PAC1 antagonist, show that VIP and PACAP as well as the PAC1 receptor are involved in neutrophil recruitment in different target organs, in adhesion molecules expression, and in coagulation-related molecule fibrinogen synthesis. Thus, this study provides some important insights with respect to the involvement of PAC1 into the complexities of sepsis and represents an advantage for the design of more specific drugs complementing standard intensive care therapy in severe sepsis, confirming VIP and PACAP as candidates for multitarget therapy of septic shock.
Assuntos
Reação de Fase Aguda/imunologia , Infiltração de Neutrófilos/imunologia , Óxido Nítrico/imunologia , Peroxidase/imunologia , Receptores de Superfície Celular/imunologia , Choque Séptico/imunologia , Animais , Fibrinogênio/metabolismo , Molécula 1 de Adesão Intercelular/imunologia , Intestinos/imunologia , Lipopolissacarídeos/toxicidade , Fígado/imunologia , Masculino , Camundongos , Camundongos Endogâmicos C57BL , Camundongos Knockout , Fatores de Crescimento Neural/imunologia , Neuropeptídeos/imunologia , Neurotransmissores/imunologia , Infiltração de Neutrófilos/efeitos dos fármacos , Polipeptídeo Hipofisário Ativador de Adenilato Ciclase , Receptores de Superfície Celular/genética , Receptores de Polipeptídeo Hipofisário Ativador de Adenilato Ciclase , Receptores de Polipeptídeo Hipofisário Ativador de Adenilato Ciclase , Receptores Tipo II de Peptídeo Intestinal Vasoativo , Receptores Tipo I de Polipeptídeo Intestinal Vasoativo , Proteína Amiloide A Sérica/imunologia , Molécula 1 de Adesão de Célula Vascular/imunologia , Peptídeo Intestinal Vasoativo/imunologiaRESUMO
Orexin-A is a novel neuropeptide produced by neurons mainly located in lateral hypothalamic area that potently facilitates appetite and food intake. The purpose of this study was to investigate the possible change in orexin-A immunoreactivity in suckling-induced hyperphagia. By using immunohistochemistry and image analysis techniques we examined orexin-A-like immunoreactivity in a series of rat brain sections corresponding to the hypothalamus in groups of non-lactating, lactating, lactating with overnight cessation of suckling, lactating and cessation followed by resumed short-term sucklings. Long-term lactation significantly increased daily food intake on day 3 (81%) and day 11 (180%) postpartum compared to that in non-lactating postpartum rats, whereas daily food intake was significantly decreased by overnight cessation of suckling on day 11 postpartum in long-term lactating rats (45%). Moreover, long-term lactating rats on day 12 postpartum exhibited significantly greater number and higher mean staining intensity of orexin-A immunoreactive neurons than those of non-suckling postpartum rats (P<0.001 and P<0.05, respectively). Overnight cessation of lactation in rats on day 12 postpartum significantly decreased both the number and mean staining intensity of orexin-A immunoreactive neurons compared to those in long-term lactating group of rats (P<0.001 and P<0.05, respectively), similar to the levels in the non-lactating postpartum rats. Resumed lactation for 2 and 5 h after overnight cessation of lactation significantly increased the number (P<0.001 and P<0.05, respectively) and mean staining intensity (P<0.05) of orexin-A immunoreactive neurons compared to those in the rats without resumed lactation. Both long-term lactation and short-term resumed suckling enhanced orexin-A immunoreactivity in the hypothalamus in rats, and overnight cessation of lactation down-regulated the increased orexin-A immunoreactivity induced by long-term lactation. Suckling may regulate orexin-A expression in the hypothalamus and the increased orexin-A may be involved in hyperphagia in lactating rats, suggesting the possibility of the existence of some neural-humoral links between suckling and hypothalamic orexin-A-immunoreactive neurons.
Assuntos
Comportamento Alimentar/fisiologia , Hipotálamo/fisiologia , Peptídeos e Proteínas de Sinalização Intracelular/metabolismo , Neuropeptídeos/metabolismo , Neurotransmissores/metabolismo , Animais , Feminino , Hiperfagia/fisiopatologia , Hipotálamo/metabolismo , Imuno-Histoquímica , Peptídeos e Proteínas de Sinalização Intracelular/imunologia , Masculino , Neurônios/metabolismo , Neurônios/fisiologia , Neuropeptídeos/imunologia , Neurotransmissores/imunologia , Orexinas , Ratos , Ratos WistarRESUMO
The loss of lean body mass (muscle wasting) is initiated by cascades or events that precipitate increased proteolysis. Muscle wasting is stimulated by internal and external factors. Humorally related feedback loops stimulated by disease states, e.g., cancer, inflammatory myopathies, leukemia, or sepsis, and initiated as a response to systemic inflammatory response syndrome can cause a downward progression of events, resulting in chronic illness or even death. This article discusses the pathogenesis of muscle wasting, which is often referred to as cachexia.