RESUMO
The aim of the present study was to evaluate the effect of silver nanoparticles (AgNPs) on Enchytraeus crypticus, applying a combined toxicokinetics and toxicodynamics approach to understand the relationship between survival and the development of internal Ag concentrations in the animals over time. Toxicity tests were conducted in medium composed of well-defined aqueous solutions added to inert quartz sand to avoid the complexity of soil conditions. Citrate-coated AgNPs (AgNP-Cit) and polyvinylpyrrolidone-coated AgNPs (AgNP-PVP) were tested and compared with silver nitrate (AgNO3), which was used as a positive control for Ag ion effects. The median lethal concentration (LC50) values based on Ag concentrations in the solution phase of the test medium decreased over time and reached steady state after 7 d, with AgNO3 and AgNP-PVP being more toxic than AgNP-Cit. Slow dissolution may explain the low uptake kinetics and lower toxicity of AgNP-Cit compared with the other 2 Ag forms. The LC50 values based on internal Ag concentrations in the animals were almost stable over time, highlighting the importance of integrating toxicokinetics and toxicodynamics and relating survival with internal Ag concentrations. Neither survival-based elimination rates nor internal LC50s in the organisms showed any significant evidence of nano-specific effects for both AgNPs, although they suggested some uptake of particulate Ag for AgNP-Cit. The authors conclude that the toxicity of both types of AgNP probably is mainly attributable to the release of Ag ions.
Assuntos
Nanopartículas Metálicas/toxicidade , Modelos Biológicos , Oligoquetos/efeitos dos fármacos , Nitrato de Prata/toxicidade , Prata/toxicidade , Poluentes do Solo/toxicidade , Animais , Citratos/farmacocinética , Citratos/toxicidade , Dose Letal Mediana , Oligoquetos/metabolismo , Povidona/farmacocinética , Povidona/toxicidade , Dióxido de Silício/farmacocinética , Dióxido de Silício/toxicidade , Prata/farmacocinética , Nitrato de Prata/farmacocinética , Poluentes do Solo/farmacocinética , Testes de ToxicidadeRESUMO
Various factors have been invoked to explain the toxicity of silver nanoparticles (AgNP) to microorganisms including particle size and the nature of stabilizing coatings as well as the amount of dissolved silver occurring in AgNP suspensions. In this study we have assessed the effects of nine differently coated AgNP (chitosan, lactate, polyvinylpyrrolidone, polyethelene glycol, gelatin, sodium dodecylbenzenesulfonate, citrate, dexpanthenol, and carbonate) and AgNO3 on the photosynthesis of the freshwater algae Chlamydomonas reinhardtii. We have thus examined how AgNP effects on algae relate to particle size, measured dissolved silver (Agd), and bioavailable silver (Agbioav). Agbioav was indirectly estimated in toxicity experiments by cysteine-silver complexation at the EC50. The EC50 calculated as a function of measured Agd concentrations showed for some coatings values similar to that of dissolved Ag, whereas other coated AgNP displayed lower EC50 values. In all cases, excess cysteine completely prevented effects on photosynthetic yield, confirming the role of Agd as a cause of the observed effect on the photosynthesis. Toxicity was related neither to particle size nor to the coatings. For all differently coated AgNP suspensions, the EC50 values calculated as a function of Agbioav were comparable to the value of AgNO3. Depending on the coatings Agbioav was comparable to or higher than measured Agd.
Assuntos
Chlamydomonas reinhardtii/efeitos dos fármacos , Nanopartículas Metálicas/química , Nanopartículas Metálicas/toxicidade , Fotossíntese/efeitos dos fármacos , Prata/toxicidade , Benzenossulfonatos/química , Benzenossulfonatos/toxicidade , Carbonatos/química , Carbonatos/toxicidade , Quitosana/química , Quitosana/toxicidade , Chlamydomonas reinhardtii/fisiologia , Citratos/química , Citratos/toxicidade , Cisteína/farmacologia , Cisteína/toxicidade , Gelatina/química , Gelatina/toxicidade , Lactatos/química , Lactatos/toxicidade , Ácido Pantotênico/análogos & derivados , Ácido Pantotênico/química , Ácido Pantotênico/toxicidade , Tamanho da Partícula , Polietilenoglicóis/química , Polietilenoglicóis/toxicidade , Povidona/toxicidade , Prata/farmacocinética , Nitrato de Prata/farmacocinética , Testes de Toxicidade/métodosRESUMO
The release of Ag nanoparticles (AgNPs) into the aquatic environment is likely, but the influence of water chemistry on their impacts and fate remains unclear. Here, we characterize the bioavailability of Ag from AgNO(3) and from AgNPs capped with polyvinylpyrrolidone (PVP AgNP) and thiolated polyethylene glycol (PEG AgNP) in the freshwater snail, Lymnaea stagnalis, after short waterborne exposures. Results showed that water hardness, AgNP capping agents, and metal speciation affected the uptake rate of Ag from AgNPs. Comparison of the results from organisms of similar weight showed that water hardness affected the uptake of Ag from AgNPs, but not that from AgNO(3). Transformation (dissolution and aggregation) of the AgNPs was also influenced by water hardness and the capping agent. Bioavailability of Ag from AgNPs was, in turn, correlated to these physical changes. Water hardness increased the aggregation of AgNPs, especially for PEG AgNPs, reducing the bioavailability of Ag from PEG AgNPs to a greater degree than from PVP AgNPs. Higher dissolved Ag concentrations were measured for the PVP AgNPs (15%) compared to PEG AgNPs (3%) in moderately hard water, enhancing Ag bioavailability of the former. Multiple drivers of bioavailability yielded differences in Ag influx between very hard and deionized water where the uptake rate constants (k(uw), l g(-1) d(-1) ± SE) varied from 3.1 ± 0.7 to 0.2 ± 0.01 for PEG AgNPs and from 2.3 ± 0.02 to 1.3 ± 0.01 for PVP AgNPs. Modeling bioavailability of Ag from NPs revealed that Ag influx into L. stagnalis comprised uptake from the NPs themselves and from newly dissolved Ag.
Assuntos
Dureza , Lymnaea/metabolismo , Nanopartículas Metálicas/química , Nitrato de Prata/química , Nitrato de Prata/farmacocinética , Prata/química , Prata/farmacocinética , Poluentes Químicos da Água/farmacocinética , Animais , Disponibilidade Biológica , Cátions Bivalentes/química , Cátions Bivalentes/farmacocinética , Água Doce , Lymnaea/efeitos dos fármacos , Polietilenoglicóis/química , Povidona/química , Água , Poluentes Químicos da Água/químicaRESUMO
The study of the bioconcentration of silver nanoparticles (AgNPs) is important to fully understand their hazard potential in the aquatic environment. We synthesized AgNPs radiolabeled with silver isotopes ((110m)Ag) to quantify the bioconcentration of AgNPs coated with citrate (AgNPs-CIT) and polyvinylpyrrolidone (AgNPs-PVP) in Japanese medaka, and to investigate the biodistribution of silver in organs, which were compared with (110m)AgNO3. BCF values were determined to be 39.8±7.4, 42.5±5.1 and 116.4±6.1Lkg(-1) for AgNPs-CIT, AgNPs-PVP and AgNO3, respectively. The release of more silver ions in AgNPs-PVP contributed to a different kinetic uptake pattern with AgNPs-CIT, which was similar to that of AgNO3. Bioconcentrated AgNPs in medaka were not observed to be eliminated, independent of surface coating differences, similarly to AgNO3. There was no difference in biodistribution in each organ before and after depuration in two types of AgNPs and AgNO3, all of which were mainly concentrated in the liver. This study quantified the bioconcentration and distribution of AgNPs and AgNO3 more precisely by utilizing a silver isotope, which is helpful in monitoring the toxicity of AgNPs to Japanese medaka.
Assuntos
Nanopartículas Metálicas , Nanopartículas/metabolismo , Oryzias/metabolismo , Compostos de Prata/metabolismo , Algoritmos , Animais , Eletroquímica , Fígado/metabolismo , Microscopia Eletrônica de Transmissão , Especificidade de Órgãos , Tamanho da Partícula , Povidona , Radioisótopos , Nitrato de Prata/farmacocinética , Propriedades de Superfície , Distribuição TecidualRESUMO
In aqueous media, ionic silver concentrations are low and transport occurs in the colloidal phase. In the aquatic environment, silver forms 1:1 complexes with thiol-containing compounds such as cysteine and glutathione. In order to quantitatively characterize the risk associated with silver in aquatic ecosystems, the bioavailabilities and toxicities of silver cysteinate and silver glutathionate were characterized. Static renewal bioassays were conducted with Ceriodaphnia dubia to estimate chronic toxicity, using mortality and reproduction as endpoints. Silver nitrate was the most lethal compound, with a median lethal concentration (8-d LC50) of 0.32 microg Ag/L (95% confidence interval [CI] = 0.19-0.54). The 48-h LC50 for AgNO3 was 0.5 microg/L and did not change significantly through 8 d. The presence of food in the bioassay did not change the 48-h LC50 for AgNO3. Silver glutathionate (AgGSH) and silver cysteinate (AgCys) induced less mortality during the 8-d bioassay. Silver cysteinate appeared to have the greatest effect on fecundity, with a no-observable-effect concentration (NOEC) less than 0.001 microg/L. Silver nitrate and AgGSH had lowest-observable-effect concentration (LOEC) values (nominal concentrations) of 0.01 and 0.6 microg/L, respectively. Results indicate that the ligand-bound silver in these laboratory studies is bioavailable and impairs reproduction of C. dubia at low aqueous concentrations.
Assuntos
Crustáceos/efeitos dos fármacos , Prata/toxicidade , Poluentes Químicos da Água/toxicidade , Animais , Crustáceos/metabolismo , Cisteína/química , Fertilidade/efeitos dos fármacos , Glutationa/química , Dose Letal Mediana , Ligantes , Nível de Efeito Adverso não Observado , Prata/química , Prata/farmacocinética , Nitrato de Prata/farmacocinética , Nitrato de Prata/toxicidade , Poluentes Químicos da Água/farmacocinéticaRESUMO
Exposure to elevated waterborne silver as AgNO3 (4.07 microM=448 microg l(-1)) in seawater resulted in osmoregulatory disturbance in the lemon sole (Parophrys vetulus). The main effects were increased plasma Na+ and Cl- concentrations which translated into increased plasma osmolality. Plasma Mg2+ levels were also slightly increased after 96 h exposure. Using radioisotopic flux measurements, a 50% reduction in branchial unidirectional Na+ extrusion was observed after 48 h silver exposure. By applying an intestinal perfusion approach, we were able to separate and thus quantify the intestinal contribution to the observed silver-induced physiological disturbance and internal silver accumulation. This analysis revealed that the intestinal contribution to silver-induced ionoregulatory toxicity was as high as 50-60%. In marked contrast, internal silver accumulation (in liver and kidney) was found to be derived exclusively from uptake across the gills. Drinking of silver-contaminated seawater resulted in substantial silver accumulation in the intestinal tissue (but apparently not silver uptake across the intestine), which probably explains the intestinal contribution to silver-induced physiological disturbance.