Preparation of porous magnetic molecularly imprinted polymers for fast and specifically extracting trace norfloxacin residue in pork liver.

Here, magnetic molecularly imprinted polymers were designed for norfloxacin via oil-in-water emulsifier-free emulsion method. It was prepared by simply mixing norfloxacin, methacrylic acid-co-ethylene glycol dimethacrylate copolymer, and Fe3 O4 together at room temperature. Characterized by multiple analytical tools, the particle size, pore size, pore volume, specific surface area, and saturation magnetization of the product were about 30 µm, 10-500 nm, 2.92 mL/g, 105.84 m2 /g, and 3.052 emu/g, respectively. And the adsorption capacity was high at 27.04 mg/g towards norfloxacin. Combined with ultra high performance liquid chromatography, it was used to determine norfloxacin in real samples. Average recoveries were above 77.44% with relative standard deviations between 1.21 and 6.85% at three spiked levels (n = 3) for lake water and pork liver. The determination was achieved for the most complex biosample pork liver spiked with norfloxacin low to 30 ng/g, about 100 times less than the maximum residue limit regulated by Commission of the European Communities. All evidences demonstrated that the magnetic molecularly imprinted polymers can be used in practice for monitoring norfloxacin either in environmental water or meat product with high accuracy and reliability.

Avaliaçäo in vitro da sensibilidade bacteriana à fluorquinolona norfloxacina em infecçöes oculares em serviço de referência / Bacterial susceptibility in vitro evaluation of the fluorquinolone norflaxacin in ocular infections in a reference service

Foi analisada a sensibilidade in vitro à fluorquinolona norfloxacina de 224 microrganismos isolados de 213 culturas consecutivas obtidas junto ao Laboratório de Patologia Externa da Escola Paulista de Medicina no período de agosto de 1989 a julho de 1990. Os microorganismos foram isolados de infecçöes oculares (blefarite, blefaroconjuntivite, conjuntivite, úlcera de córnea, dacriocistite, endoftalmite e meibomite), sendo submetidos ao antibiograma para norfloxacina, tobramicina, gentamicina, cefalotina e amicacina. A norfloxacina foi eficaz contra S. epidemidis, teve atividade similar à gentamicina contra espécies de Pseudomonas e Haemophilus. Sua eficácia ficou aquém da gentamicina para P. mirabilis e P.vugaris. Também näo mostrou atividade superior à cefalotina para S.aureus e S.pneumoniae