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1.
Curr Microbiol ; 79(4): 124, 2022 Mar 08.
Artigo em Inglês | MEDLINE | ID: mdl-35258703

RESUMO

A novel Gram-negative, motile, aerobic, spiral-shaped bacterium designated D5T, was isolated from a coastal sediment collected in the Yellow Sea. Optimal growth occurred at 30 °C, pH 7.0-8.0 and in the presence of 1-3% (w/v) NaCl. Strain D5T contained ubiquinone 8 (Q-8) as the predominant respiratory quinone. The major fatty acids (> 10%) were C16:0, C16:1 ω7c/C16:1 ω6c and C18:1w7c/C18:1w6c. The main polar lipids were phosphatidylglycerol and phosphatidylethanolamine. The draft genome is 5.6 Mb in length, and DNA G + C content is 47.2 mol%. 16S rRNA gene sequences showed that strain D5T is most closely related to Oceanospirillum beijerinckii NBRC 15445T (97.8%, sequence similarity). However, the digital DNA-DNA hybridization (dDDH) value and average nucleotide identity (ANI) between strain D5T and O. beijerinckii is only 27.8% and 77.1%. Phylogenetic trees based on 16S rRNA gene sequences and whole genomes all indicated that strain D5T formed a separate branch in the genus Oceanospirillum. Combined results of the polyphasic analyses suggested that strain D5T represents a novel species in the genus Oceanospirillum, for which the name Oceanospirillum sediminis sp. nov. is proposed. The type strain is D5T (= MCCC 1K06061T = KCTC 62987T).


Assuntos
Sedimentos Geológicos , Oceanospirillaceae , Filogenia , Água do Mar , Técnicas de Tipagem Bacteriana , DNA Bacteriano/genética , Ácidos Graxos/química , Sedimentos Geológicos/microbiologia , Oceanospirillaceae/classificação , Oceanospirillaceae/isolamento & purificação , Fosfolipídeos/química , RNA Ribossômico 16S/genética , Água do Mar/microbiologia , Análise de Sequência de DNA , Ubiquinona/química
2.
Arch Microbiol ; 201(8): 1053-1060, 2019 Oct.
Artigo em Inglês | MEDLINE | ID: mdl-31115641

RESUMO

Strain HPM-16T, isolated from seawater, was characterized using a polyphasic taxonomy approach. Phylogenetic analyses based on 16S rRNA gene sequences and coding sequences of an up-to-date bacterial core gene set (92 protein clusters) indicated that strain HPM-16T formed a phylogenetic lineage in the genus Neptunomonas. Strain HPM-16T was most closely related to Neptunomonas concharum LHW37T with 16S rRNA gene sequence similarity of 96.7%. Cells were Gram-stain negative, facultatively anaerobic, motile by means of a single polar flagellum, rod-shaped and formed white colonies. Optimal growth occurred at 30-35 °C, pH 6.5-8, and in the presence of 2-5% NaCl. C18:1ω7c and summed feature 3 (C16:1ω7c and/or C16:1ω6c) were the predominant fatty acids. The only isoprenoid quinone was Q-8. The polar lipid profile revealed the presence of phosphatidylethanolamine, phosphatidylglycerol and several uncharacterized lipids. The major polyamines were putrescine and spermidine. The draft genome was approximately 3.68 Mb in size with a G + C content of 50.5 mol%. Differential phenotypic properties, together with the phylogenetic inference, demonstrate that strain HPM-16T should be classified as a novel species of the genus Neptunomonas, for which the name Neptunomonas marina sp. nov. is presented. The type strain is HPM-16T (= BCRC 80980T = LMG 29560T = KCTC 52235T).


Assuntos
Oceanospirillaceae , Água do Mar/microbiologia , Técnicas de Tipagem Bacteriana , Composição de Bases/genética , DNA Bacteriano/genética , Ácidos Graxos/análise , Oceanospirillaceae/classificação , Oceanospirillaceae/genética , Oceanospirillaceae/isolamento & purificação , Fosfatidiletanolaminas/análise , Fosfatidilgliceróis/análise , Fosfolipídeos/análise , Filogenia , Quinonas/análise , RNA Ribossômico 16S/genética , Análise de Sequência de DNA
3.
Int J Syst Evol Microbiol ; 68(2): 651-656, 2018 Feb.
Artigo em Inglês | MEDLINE | ID: mdl-29303694

RESUMO

A Gram-stain-negative, aerobic, motile, flagellated rod-shaped bacterium, designated ST58-10T, was isolated from an estuarine sediment in the Republic of Korea. The strain was able to degrade benzene. Growth of strain ST58-10T was observed at 4-35 °C (optimum, 20-25 °C), pH 5-9 (optimum, pH 7-8) and 1-8 % NaCl (optimum, 3 %). Phylogenetic analyses based on 16S rRNA gene sequences showed that strain ST58-10T formed a phyletic lineage within the genus Marinobacterium of the family Oceanospirillaceae. Strain ST58-10T was most closely related to Marinobacterium profundum PAMC 27536T (99.6 %) and Marinobacterium rhizophilum CL-YJ9T (98.3 %), and to other members of the genus Marinobacterium(94.5-91.5 %). However, the mean value estimated by using the Genome-to-Genome Distance Calculator was 50.6±7.4 % with M. profundum PAMC 27536T and 30.9±2.8 with M. rhizophilum CL-YJ9T, respectively. An average nucleotide identity value was 89.0 % with M. profundum PAMC 27536T and 85.6 % with M. rhizophilum CL-YJ9T, respectively. The major fatty acids of strain ST58-10T were summed feature 3 (comprising C16 : 1ω7c/C16 : 1ω6c), summed feature 8 (comprising C18 : 1 ω7c/C18 : 1ω6c), C16 : 0 and C10 : 0 3-OH, and contained ubiquinone (Q-8) as the sole isoprenoid quinone. Phosphatidylethanolamine, phosphatidylglycerol, three unidentified aminolipids, an unidentified glycolipid and an unidentified lipid were detected as polar lipids. The DNA G+C content of strain ST58-10T was 58.78 mol%. On the basis of the phenotypic, chemotaxonomic and molecular properties, strain ST58-10T represents a novel species of the genus Marinobacterium, for which the name Marinobacterium aestuarii sp. nov. is proposed. The type strain is ST58-10T (=KCTC 52193T=NBRC 112103T).


Assuntos
Estuários , Sedimentos Geológicos/microbiologia , Oceanospirillaceae/classificação , Filogenia , Água do Mar/microbiologia , Técnicas de Tipagem Bacteriana , Composição de Bases , Benzeno , DNA Bacteriano/genética , Ácidos Graxos/química , Oceanospirillaceae/genética , Oceanospirillaceae/isolamento & purificação , Fosfolipídeos/química , RNA Ribossômico 16S/genética , República da Coreia , Análise de Sequência de DNA , Ubiquinona/química
4.
Int J Syst Evol Microbiol ; 68(1): 432-437, 2018 Jan.
Artigo em Inglês | MEDLINE | ID: mdl-29235976

RESUMO

Strain Eup a-2T, isolated from the torch coral Euphyllia glabrescens, was characterized using a polyphasic taxonomy approach. Cells of strain Eup a-2T were Gram-negative, aerobic and motile by three polar flagella and formed translucent colonies. Optimal growth occurred at 25 °C, pH 6-8 and in the presence of 2-4 % NaCl. Phylogenetic analyses based on 16S rRNA gene sequences showed that strain Eup a-2T belonged to the genus Litoribrevibacter and showed the highest levels of sequence similarity with respect to Litoribrevibacter albus Y32T (97.8 %). Strain Eup a-2T contained summed feature 3 (C16 : 1ω7c and/or C16 : 1ω6c) and C16 : 0 as the predominant fatty acids. The predominant isoprenoid quinone was Q-8. The major polar lipids were phosphatidylethanolamine, phosphatidylglycerol and diphophatidylglycerol. Genomic DNA G+C content of strain Eup a-2T was 49.1 mol%. The DNA-DNA hybridization value for strain Eup a-2T with L. albus Y32T was less than 30 %. Differential phenotypic properties, together with the phylogenetic inference, demonstrate that strain Eup a-2T should be classified as a novel species of the genus Litoribrevibacter, for which the name Litoribrevibactereuphylliae sp. nov. is presented. The type strain is Eup a-2T (=BCRC 81004T=LMG 29725T=KCTC 52438T).


Assuntos
Antozoários/microbiologia , Oceanospirillaceae/classificação , Filogenia , Animais , Técnicas de Tipagem Bacteriana , Composição de Bases , DNA Bacteriano/genética , Ácidos Graxos/química , Hibridização de Ácido Nucleico , Oceanospirillaceae/genética , Oceanospirillaceae/isolamento & purificação , Fosfolipídeos/química , RNA Ribossômico 16S/genética , Análise de Sequência de DNA , Vitamina K 2/análogos & derivados , Vitamina K 2/química
5.
Syst Appl Microbiol ; 40(2): 80-85, 2017 Mar.
Artigo em Inglês | MEDLINE | ID: mdl-28040300

RESUMO

Nine isolates obtained from a great scallop hatchery in Norway were characterized using a polyphasic approach. Strains were Gram-negative, aerobic and motile rods with oxidative metabolism. Phylogenetic analysis based on the sequences of 16S rRNA and rpoB genes showed that these strains formed two different groups associated with members of the genus Neptuniibacter. DNA-DNA hybridization (DDH) and Average Nucleotide Identity (ANI) demonstrated that the isolates constituted two novel species of this genus, which can be phenotypically differentiated from their closest relatives. The names Neptuniibacter marinus sp. nov. and Neptuniibacter pectenicola sp. nov are proposed, with ATR 1.1T (=CECT 8938T=DSM 100783T) and LFT 1.8T (=CECT 8936T=DSM 100781T) as respective type strains.


Assuntos
Oceanospirillaceae/classificação , Oceanospirillaceae/isolamento & purificação , Pecten/microbiologia , Aerobiose , Animais , Técnicas de Tipagem Bacteriana , Análise por Conglomerados , DNA Bacteriano/química , DNA Bacteriano/genética , DNA Ribossômico/química , DNA Ribossômico/genética , RNA Polimerases Dirigidas por DNA/genética , Locomoção , Noruega , Hibridização de Ácido Nucleico , Oceanospirillaceae/genética , Oceanospirillaceae/fisiologia , Fosfolipídeos/análise , Filogenia , RNA Ribossômico 16S/genética , Análise de Sequência de DNA
6.
Int J Syst Evol Microbiol ; 66(12): 5010-5015, 2016 Dec.
Artigo em Inglês | MEDLINE | ID: mdl-27566052

RESUMO

A Gram-staining-negative, strictly aerobic, transparent and smooth, curved-rod-shaped motile bacterium with a single polar flagellum, designated strain sw100T, was isolated from surface seawater of South Pacific Gyre during Integrated Ocean Drilling Program Expedition 329. Phylogenetic analysis based on 16S rRNA gene sequences indicated that strain sw100T was most closely related to the member of the family Oceanospirillaceae and was distinct from the most closely related genera Bacterioplanes, Thalassolituus and Oceanobacter (95.8, 94.5-95.3 and 94.4 % 16S rRNA gene sequence similarities, respectively). Growth occurred in the presence of 0-11 % NaCl (w/v, optimum 7 %), at 10-37 °C (optimum 28 °C) and pH 6.0-9.0 (optimum pH 7.0). The major fatty acids (>10 % of total fatty acids) are C16 : 0, C16 : 0 N alcohol and summed feature 3 (C16 : 1ω6c and/or C16 : 1ω7c). The major polar lipids were phosphatidylethanolamine, phosphatidylmonomethylethanolamine, phosphatidylglycerol, diphosphatidylglycerol and two unidentified polar lipids. The DNA G+C content of strain sw100T was 55.3 mol%. The major respiratory quinone was ubiquinone-10 (Q-10). On the basis of polyphasic analyses, strain sw100T is considered to represent a novel species in a novel genus of the family Oceanospirillaceae, for which the name Bacterioplanoides pacificum gen. nov., sp. nov. is proposed. The type strain is sw100T (=JCM 30597T=MCCC 1K00501T=KCTC 42424T).


Assuntos
Oceanospirillaceae/classificação , Filogenia , Água do Mar/microbiologia , Microbiologia da Água , Técnicas de Tipagem Bacteriana , Composição de Bases , DNA Bacteriano/genética , Ácidos Graxos/química , Oceanospirillaceae/genética , Oceanospirillaceae/isolamento & purificação , Oceano Pacífico , Fosfolipídeos/química , RNA Ribossômico 16S/genética , Análise de Sequência de DNA , Ubiquinona/química
7.
Syst Appl Microbiol ; 39(5): 313-8, 2016 Jul.
Artigo em Inglês | MEDLINE | ID: mdl-27344411

RESUMO

Two gram-negative, facultative anaerobic, chemoorganoheterotrophic, motile and rod-shaped bacteria, strains AVMART05(T) and KASP37, were isolated from ascidians (Tunicata, Ascidiaceae) of the genus Ascidiella collected at Gullmarsfjord, Sweden. The strains are the first cultured representatives of an ascidian-specific lineage within the genus Endozoicomonas (Gammaproteobacteria, Oceanospirillales, Hahellaceae). Both strains feature three distinct 16S rRNA gene paralogs, with identities of 98.9-99.1% (AVMART05(T)) and 97.7-98.8% (KASP37) between paralogs. The strains are closely related to Endozoicomonas atrinae and Endozoicomonas elysicola, with which they share 97.3-98.0% 16S rRNA gene sequence identity. Digital DNA-DNA hybridization, average nucleotide identity, and tetra-nucleotide correlation analysis indicate that both strains belong to a single species distinct from their closest relatives. Both strains feature similar DNA G+C contents of 46.70mol% (AVMART05(T)) and 44.64mol% (KASP37). The fatty acid patterns of AVMART05(T) and KASP37 are most similar to those of Endozoicomonas euniceicola and Endozoicomonas gorgoniicola. Based on the polyphasic approach, we propose the species Endozoicomonas ascidiicola sp. nov. to accommodate the newly isolated strains. E. ascidiicola sp. nov. is represented by the type strain AVMART05(T) (=DSM 100913(T)=LMG 29095(T)) and strain KASP37 (=DSM 100914=LMG 29096).


Assuntos
Oceanospirillaceae , RNA Ribossômico 16S/genética , Urocordados/microbiologia , Animais , Técnicas de Tipagem Bacteriana , Composição de Bases , Sequência de Bases , DNA Bacteriano/genética , Ácidos Graxos/análise , Hibridização de Ácido Nucleico , Oceanospirillaceae/classificação , Oceanospirillaceae/genética , Oceanospirillaceae/isolamento & purificação , Filogenia , Análise de Sequência de DNA , Suécia
8.
Syst Appl Microbiol ; 38(8): 555-62, 2015 Dec.
Artigo em Inglês | MEDLINE | ID: mdl-26481633

RESUMO

The novel, cream coloured, Gram-negative-staining, rod-shaped, motile bacteria, designated strains AK23(T) and AK28, were isolated from sediment samples collected from Lonar Lake, Buldhana district, India. The predominant fatty acids were C18:1ω7c, C16:0, C10:0 3OH and C16:1ω7c and/or iso-C15:0 2OH (summed feature 3). Polar lipid content of strains AK23(T) and AK28 were found to be phosphatidylethanolamine (PE), phosphatidylglycerol (PG), phosphotidylserine (PS), one unidentified phospholipid (PL) and two unidentified lipids (L1 and L2). The 16S rRNA gene sequence analysis indicated strains AK23(T) and AK28 as the members of the genus Nitrincola and closely related to the type strain Nitrincola lacisaponensis with pair-wise sequence similarity of 97.67% and 97.62% respectively. DNA-DNA hybridization between strain AK23(T) and AK28 showed a relatedness of 91%. Genome of strains AK23(T) and N. lacisaponensis DSM 16316(T) were sequenced. A comparative genomics approach was used to study strains AK23(T), N. lacisaponensis DSM 16316(T) and five other phylogenetic neighbours. The genome size of N. lacisaponensis DSM 16316(T) was found to be 614,784bp smaller than that of the strain AK23(T). This variation could be due to multiple reasons, gene uptake, evolution, mutation, genome reduction phenomenon and draft nature of sequencing. Based on data from the current polyphasic study, strains AK23(T) and AK28 are proposed as novel species of the genus Nitrincola, for which the name Nitrincola nitratireducens sp. nov. is proposed. The type strain of N. nitratireducens is AK23(T) (=JCM 18788(T)=MTCC 11628(T)).


Assuntos
Lagos/microbiologia , Oceanospirillaceae/classificação , Oceanospirillaceae/isolamento & purificação , Técnicas de Tipagem Bacteriana , Análise por Conglomerados , Citosol/química , DNA Bacteriano/química , DNA Bacteriano/genética , DNA Ribossômico/química , DNA Ribossômico/genética , Ácidos Graxos/análise , Genoma Bacteriano , Sedimentos Geológicos/microbiologia , Índia , Locomoção , Dados de Sequência Molecular , Hibridização de Ácido Nucleico , Oceanospirillaceae/genética , Oceanospirillaceae/fisiologia , Fosfolipídeos/análise , Filogenia , Pigmentos Biológicos/metabolismo , RNA Ribossômico 16S/genética , Análise de Sequência de DNA
9.
Int J Syst Evol Microbiol ; 65(Pt 6): 1866-1870, 2015 Jun.
Artigo em Inglês | MEDLINE | ID: mdl-25766110

RESUMO

A Gram-stain-negative, rod-shaped (1.2-2.1 µm × 0.8-0.9 µm), flagellated and motile marine bacterium, designated MEBiC05461T, was isolated from a marine sponge inhabiting Micronesia. Strain MEBiC05461T was oxidase-negative and catalase-positive. Growth was observed at 8.0-35.6 °C (optimum 30.0 °C), at pH 5.0-9.0 (optimum pH 7.0) and with 1.5-6.0 % (w/v, optimum 2.0-2.5 %) NaCl. 16S rRNA gene sequence analysis revealed that strain MEBiC05461T showed high similarity to members of the genus Amphritea (96.4-96.6 %). The predominant cellular fatty acids were C16:0 (23.9 %), summed feature 3 (C16:1ω7c and/or C16:1ω6c; 39.7 %) and summed feature 8 (C18:1ω7c and/or C18:1ω6c; 22.0 %). The DNA G+C content was 48.5 mol%. The major respiratory quinone was Q-8.Phosphatidylethanolamine, phosphatidylglycerol, one unidentified glycolipid, one unidentified aminolipid, one unidentified glycophospholipid and two unidentified lipids were detected as the major polar lipids. On the basis of the data from this polyphasic taxonomic study, strain MEBiC05461T should be classified as a representative of a novel species in the genus Amphritea, and the name proposed is Amphritea spongicola sp. nov. The type strain is MEBiC05461T ( = KCCM 42943T = JCM 16668T). Emendations of the genus Amphritea and species Amphritea atlanticaGärtner et al. 2008 and Amphritea balenaeMiyazaki et al. 2008 are were also given.


Assuntos
Oceanospirillaceae/classificação , Filogenia , Poríferos/microbiologia , Animais , Técnicas de Tipagem Bacteriana , Composição de Bases , DNA Bacteriano/genética , Ácidos Graxos/química , Glicolipídeos/química , Micronésia , Dados de Sequência Molecular , Oceanospirillaceae/genética , Oceanospirillaceae/isolamento & purificação , Fosfatidiletanolaminas/química , Fosfatidilgliceróis/química , RNA Ribossômico 16S/genética , Água do Mar , Análise de Sequência de DNA , Ubiquinona/química
10.
Int J Syst Evol Microbiol ; 65(Pt 3): 915-919, 2015 Mar.
Artigo em Inglês | MEDLINE | ID: mdl-25563909

RESUMO

A Gram-stain-negative, facultatively anaerobic, oxidase- and catalase-positive, rod-shaped bacterium, strain SYM1(T), was isolated from a culture of Symbiodinium sp., an algal symbiont of the sea anemone Aiptasia tagetes collected in Puerto Rico. Growth was observed at 4-40 °C (optimum 30 °C), at pH 5.0-11.0 (optimum pH 8.0) and with 0.5-8 % (optimum 2 %) (w/v) NaCl. Phylogenetic analyses of 16S rRNA gene sequences showed that strain SYM1(T) was a member of the genus Neptunomonas with the type strain of Neptunomonas naphthovorans as the closest phylogenetic relative with a pairwise sequence similarity of 98.15 %. However, DNA-DNA relatedness between strain SYM1(T) and N. naphthovorans CIP 106451(T) was 24 %. Moreover, strain SYM1(T) could be distinguished from its closest relative by several phenotypic characteristics such as NaCl, pH and temperature tolerance, nitrate reduction and utilization of carbon substrates. The major cellular fatty acids were C16 : 0, C18 : 1ω7c and summed feature 3 (comprising C16 : 1ω7c and/or iso-C15 : 0 2-OH). The genomic DNA G+C content of strain SYM1(T) was 45 mol%. Ubiquinone-8 (Q-8) was the only respiratory quinone detected. Based on a polyphasic taxonomic characterization, strain SYM1(T) represents a novel species of the genus Neptunomonas, for which the name Neptunomonas phycophila sp. nov. is proposed. The type strain is SYM1(T) ( = LMG 28329(T) = CECT 8716(T)).


Assuntos
Dinoflagellida/microbiologia , Oceanospirillaceae/classificação , Filogenia , Anêmonas-do-Mar/microbiologia , Animais , Técnicas de Tipagem Bacteriana , Composição de Bases , DNA Bacteriano/genética , Ácidos Graxos/química , Dados de Sequência Molecular , Hibridização de Ácido Nucleico , Oceanospirillaceae/genética , Oceanospirillaceae/isolamento & purificação , Porto Rico , RNA Ribossômico 16S/genética , Análise de Sequência de DNA , Simbiose , Ubiquinona/química
11.
Curr Microbiol ; 70(4): 514-9, 2015 Apr.
Artigo em Inglês | MEDLINE | ID: mdl-25487119

RESUMO

A strictly aerobic, Gram-negative, beige-pigmented, short-rod-shaped, non-motile and chemoheterotrophic bacteria, designated K2-48(T) was isolated from seawater collected in the Western North Pacific Ocean near Japan. Preliminary analysis based on the 16S rRNA gene sequence revealed that the novel isolate was affiliated with the family Oceanospirillaceae within the class Gammaproteobacteria and that it showed the highest sequence similarity (93.7 %) to Neptunomonas qingdaonensis P10-2-4(T). The strain could be differentiated phenotypically from recognized members of the family Oceanospirillaceae. The major fatty acids of strain K2-48(T) were identified as summed feature 3 (C16:1 ω7c and/or iso-C15:0 2-OH) and C16:0 as defined by the MIDI system. The DNA G+C content was determined to be 43.2 mol%, the major respiratory quinone was identified as ubiquinone 9 and a polar lipid profile was present consisting of phosphatidylethanolamine, a phosphatidylglycerol and an unidentified phospolipid. On the basis of polyphasic taxonomic studies, it was concluded that strain K2-48(T) represents a novel genus sp. We propose the name Pelagitalea pacifica gen. nov., sp. nov. for this strain; its type strain is K2-48(T) (=KCCM 90119(T)).


Assuntos
Oceanospirillaceae/classificação , Oceanospirillaceae/isolamento & purificação , Água do Mar/microbiologia , Organismos Aquáticos/classificação , Organismos Aquáticos/isolamento & purificação , Técnicas de Tipagem Bacteriana , Composição de Bases , Análise por Conglomerados , Citosol/química , DNA Bacteriano/química , DNA Bacteriano/genética , DNA Ribossômico/química , DNA Ribossômico/genética , Ácidos Graxos/análise , Japão , Locomoção , Dados de Sequência Molecular , Oceanospirillaceae/genética , Oceanospirillaceae/fisiologia , Oceano Pacífico , Fosfolipídeos/análise , Filogenia , Pigmentos Biológicos/metabolismo , Quinonas/análise , RNA Ribossômico 16S/genética , Análise de Sequência de DNA
12.
Int J Syst Evol Microbiol ; 64(Pt 11): 3650-3654, 2014 Nov.
Artigo em Inglês | MEDLINE | ID: mdl-25085876

RESUMO

A Gram-reaction-negative, aerobic, rod-shaped (1.2-1.6 µm×0.6-0.8 µm), flagellated and motile marine bacterium, designated MEBiC06243(T), was isolated from a sediment collected at Daebu Island in the Yellow Sea (37° 20' N 126° 41' E), Korea. The 16S rRNA gene sequence analysis revealed that strain MEBiC06243(T) showed high similarity with Neptunomonas naphthovorans NAG-2N-126(T) (96.3%). Growth was observed at 10-39 °C (optimum 29 °C), at pH 6.0-9.0 (optimum pH 7) and with 0-7% (optimum 2.5%) NaCl. The predominant cellular fatty acids were C(10:0) 3-OH (6.1%), C(12:0) (5.8%), C(16:0) (30.5%), C(18:1)ω7c (21.6%) and summed feature 3 (comprising C(15:0) 2-OH and/or C(16:1)ω7c; 30.7%). The DNA G+C content was 41.4 mol%. The major respiratory quinone was Q-8. Phosphatidylethanolamine, phosphatidylglycerol, diphosphatidylglycerol, two unidentified lipids, one unidentified aminophospholipid and three unidentified aminolipids were detected as major polar lipids. On the basis of this polyphasic taxonomic data, strain MEBiC06243(T) should be classified as a novel species of the genus Neptunomonas proposed as Neptunomonas acidivorans sp. nov. The type strain is MEBiC06243(T) ( =KCCM 42975(T) =JCM 18291(T)). An emended description of the genus Neptunomonas is also given.


Assuntos
Sedimentos Geológicos/microbiologia , Oceanospirillaceae/classificação , Filogenia , Técnicas de Tipagem Bacteriana , Composição de Bases , DNA Bacteriano/genética , Ácidos Graxos/química , Dados de Sequência Molecular , Oceanospirillaceae/genética , Oceanospirillaceae/isolamento & purificação , RNA Ribossômico 16S/genética , República da Coreia , Água do Mar/microbiologia , Análise de Sequência de DNA , Ubiquinona/química
13.
Int J Syst Evol Microbiol ; 62(Pt 5): 1104-1109, 2012 May.
Artigo em Inglês | MEDLINE | ID: mdl-21705446

RESUMO

A bacterial strain designated antisso-13(T) was isolated from a salt pan in southern Taiwan and characterized using a polyphasic taxonomic approach. Strain antisso-13(T) was gram-negative, aerobic, creamy white in colour, rod-shaped and motile by single monopolar flagellum. Phylogenetic analyses based on 16S rRNA gene sequences showed that strain antisso-13(T) belonged to the genus Neptuniibacter and its closest neighbour was Neptuniibacter caesariensis MED92(T), with 96.7 % sequence similarity. Phylogenetic analyses based on rpoB and recA gene sequences and deduced amino acid sequences also revealed that the novel strain and N. caesariensis MED92(T) formed a distinct phylogenetic cluster. Strain antisso-13(T) exhibited optimal growth at 25-30 °C, with 2.0-4.0 % NaCl and at pH 7.0. Cellular fatty acids were C(16 : 1)ω7c (39.8 %), C(18 : 1)ω7c (29.4 %), C(16 : 0) (15.5 %), C(10 : 0) 3-OH (7.5 %), anteiso-C(11 : 0) (1.8 %), C(18 : 0) (1.8 %), C(11 : 0) 2-OH (1.6 %), iso-C(10 : 0) (1.2 %) and C(14 : 0) (1.1 %). The major respiratory quinone was ubiquinone Q-8. The polar lipid profile consisted of a mixture of phosphatidylglycerol, phosphatidylethanolamine and several uncharacterized polar lipids and the DNA G+C content was 54.2 mol%. The results of physiological and biochemical tests allowed clear phenotypic differentiation of this isolate from previously described members of the genus Neptuniibacter. It is evident from the genotypic and phenotypic data that strain antisso-13(T) should be classified as a representative of a novel species in the genus Neptuniibacter. The name proposed for this taxon is Neptuniibacter halophilus sp. nov.; the type strain is antisso-13(T) ( = LMG 25378(T) = BCRC 80079(T)). An emended description of the genus Neptuniibacter is provided.


Assuntos
Sedimentos Geológicos/microbiologia , Oceanospirillaceae/classificação , Oceanospirillaceae/isolamento & purificação , Aerobiose , Técnicas de Tipagem Bacteriana , Composição de Bases , Análise por Conglomerados , DNA Bacteriano/química , DNA Bacteriano/genética , DNA Ribossômico/química , DNA Ribossômico/genética , RNA Polimerases Dirigidas por DNA/genética , Ácidos Graxos/análise , Flagelos/fisiologia , Locomoção , Dados de Sequência Molecular , Oceanospirillaceae/genética , Oceanospirillaceae/fisiologia , Fosfolipídeos/análise , Filogenia , Pigmentos Biológicos/metabolismo , Quinonas/análise , RNA Ribossômico 16S/genética , Recombinases Rec A/genética , Análise de Sequência de DNA , Cloreto de Sódio/metabolismo , Taiwan
14.
Science ; 330(6001): 204-8, 2010 Oct 08.
Artigo em Inglês | MEDLINE | ID: mdl-20736401

RESUMO

The biological effects and expected fate of the vast amount of oil in the Gulf of Mexico from the Deepwater Horizon blowout are unknown owing to the depth and magnitude of this event. Here, we report that the dispersed hydrocarbon plume stimulated deep-sea indigenous γ-Proteobacteria that are closely related to known petroleum degraders. Hydrocarbon-degrading genes coincided with the concentration of various oil contaminants. Changes in hydrocarbon composition with distance from the source and incubation experiments with environmental isolates demonstrated faster-than-expected hydrocarbon biodegradation rates at 5°C. Based on these results, the potential exists for intrinsic bioremediation of the oil plume in the deep-water column without substantial oxygen drawdown.


Assuntos
Biodegradação Ambiental , Poluição Ambiental , Gammaproteobacteria/metabolismo , Hidrocarbonetos/metabolismo , Oceanospirillaceae/metabolismo , Petróleo/metabolismo , Água do Mar/microbiologia , Biomassa , Contagem de Colônia Microbiana , Ácidos Graxos/análise , Gammaproteobacteria/classificação , Gammaproteobacteria/crescimento & desenvolvimento , Gammaproteobacteria/isolamento & purificação , Genes Bacterianos , Genes de RNAr , Dados de Sequência Molecular , Oceanospirillaceae/classificação , Oceanospirillaceae/genética , Oceanospirillaceae/isolamento & purificação , Fosfolipídeos/análise , Filogenia
15.
Int J Syst Evol Microbiol ; 60(Pt 8): 1958-1961, 2010 Aug.
Artigo em Inglês | MEDLINE | ID: mdl-19801396

RESUMO

A Gram-negative, motile, oxidase- and catalase-positive and facultatively aerobic bacterium, designated S3-22T, was isolated from marine sediment of the Nella Fjord, Antarctica. Strain S3-22T reduced nitrate to nitrite and grew at pH 6.0-8.0, at 4-25 degrees C and with 0.5-5% (w/v) NaCl. It contained Q-8 as the only respiratory quinone and summed feature 3 (C16:1omega7c and/or iso-C15:0 2-OH), C16:0 and C18:1omega7c as the major cellular fatty acids. The genomic DNA G+C content was 45.6 mol%. Phylogenetic analyses of 16S rRNA gene sequences showed that strain S3-22T was affiliated with the genus Neptunomonas, with 97.1% sequence similarity to Neptunomonas japonica JAMM 0745T and 94.8% to Neptunomonas naphthovorans NAG-2N-126T, the type strains of the only two recognized Neptunomonas species. DNA-DNA relatedness between strain S3-22T and N. japonica JCM 14595T was 20.4%. Strain S3-22T could be distinguished from the type strains of Neptunomonas species by several phenotypic properties. Based on the evidence from our polyphasic study, strain S3-22T represents a novel Neptunomonas species, for which the name Neptunomonas antarctica sp. nov. is proposed. The type strain is S3-22T (=CCTCC AB 209086T =KACC 14056T).


Assuntos
Sedimentos Geológicos/microbiologia , Oceanospirillaceae/classificação , Oceanospirillaceae/isolamento & purificação , DNA Bacteriano/genética , DNA Ribossômico/genética , Ácidos Graxos/metabolismo , Dados de Sequência Molecular , Oceanospirillaceae/genética , Oceanospirillaceae/metabolismo , Filogenia , RNA Ribossômico 16S/genética
16.
Int J Syst Evol Microbiol ; 56(Pt 2): 361-364, 2006 Feb.
Artigo em Inglês | MEDLINE | ID: mdl-16449440

RESUMO

Two aerobic, psychrohalotolerant, motile bacterial isolates, CK13T and CK16, isolated from sea-water samples collected off the subantarctic Kerguelen island, were characterized by using a polyphasic taxonomic approach. On the basis of 16S rRNA gene sequence data, the strains were 99.6% similar and exhibited 93-97% similarity with the seven recognized species of Marinomonas. The most closely related species were Marinomonas pontica and Marinomonas primoryensis, with 97 and 96 % similarity at the 16S rRNA gene sequence level, respectively. DNA-DNA hybridization values between strain CK13T and M. pontica and M. primoryensis were only 58 and 40%, respectively. The major fatty acids present in strain CK13T were iso-C(16:0), C(16:0), C(16:1)omega7c and C(18:1)omega7c. The DNA G+C content of strain CK13T was 41.2 mol%. Phosphatidylethanolamine and phosphatidylglycerol were identified as the predominant phospholipids. All the above characteristics support the affiliation of strain CK13T to the genus Marinomonas. Phylogenetic analysis and phenotypic and genotypic distinctiveness confirmed that strains CK13T and CK16 are members of a novel species of the genus Marinomonas, for which the name Marinomonas polaris sp. nov. is proposed. The type strain is CK13T (=MTCC 6645T=DSM 16579T=JCM 12522T).


Assuntos
Temperatura Baixa , Oceanospirillaceae/classificação , Água do Mar/microbiologia , Regiões Antárticas , Técnicas de Tipagem Bacteriana , DNA Bacteriano/análise , DNA Ribossômico/análise , Genes de RNAr , Genótipo , Dados de Sequência Molecular , Hibridização de Ácido Nucleico , Oceanospirillaceae/genética , Oceanospirillaceae/isolamento & purificação , Oceanospirillaceae/fisiologia , Fenótipo , Filogenia , RNA Ribossômico 16S/genética , Análise de Sequência de DNA
17.
Int J Syst Evol Microbiol ; 55(Pt 6): 2303-2307, 2005 Nov.
Artigo em Inglês | MEDLINE | ID: mdl-16280487

RESUMO

Two Gram-negative, motile, non-spore-forming, slightly halophilic bacteria (strains DSW10-10T and DSW10-19) were isolated from sea water and subjected to a polyphasic taxonomic study. They grew optimally at 30 degrees C and in the presence of 2-3 % (w/v) NaCl. Strains DSW10-10T and DSW10-19 were characterized chemotaxonomically as containing Q-8 as the predominant ubiquinone and C18 : 17c, C16 : 0 and C16 : 17c and/or iso-C15 : 0 2-OH as the major fatty acids. Their DNA G+C contents were 45 x 3-45 x 7 mol%. Strains DSW10-10T and DSW10-19 exhibited a 16S rRNA gene sequence similarity value of 100 % and possessed a mean DNA-DNA relatedness level of 85 %. Phylogenetic analyses based on 16S rRNA gene sequences showed that strains DSW10-10T and DSW10-19 fell within the radiation of the cluster encompassed by the genus Marinomonas. Strains DSW10-10T and DSW10-19 had 16S rRNA gene sequence similarity levels of 95 x 7-97 x 7 % with respect to the type strains of Marinomonas species with validly published names. Levels of DNA-DNA relatedness were low enough to indicate that the two strains constitute a distinct Marinomonas species. On the basis of phenotypic data and phylogenetic and genetic distinctiveness, strains DSW10-10T (=KCTC 12394T=DSM 17202T) and DSW10-19 were placed in the genus Marinomonas as members of a novel species, Marinomonas dokdonensis sp. nov.


Assuntos
Oceanospirillaceae/isolamento & purificação , Água do Mar/microbiologia , Cloreto de Sódio/metabolismo , Composição de Bases , DNA Bacteriano/análise , DNA Bacteriano/química , DNA Bacteriano/genética , DNA Ribossômico/análise , DNA Ribossômico/química , DNA Ribossômico/genética , Ácidos Graxos/análise , Genes de RNAr , Dados de Sequência Molecular , Oceanospirillaceae/classificação , Oceanospirillaceae/citologia , Oceanospirillaceae/fisiologia , Filogenia , RNA Ribossômico 16S/análise , RNA Ribossômico 16S/genética
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