RESUMO
Poxviruses are among the largest double-stranded DNA viruses, with members such as variola virus, monkeypox virus and the vaccination strain vaccinia virus (VACV). Knowledge about the structural proteins that form the viral core has remained sparse. While major core proteins have been annotated via indirect experimental evidence, their structures have remained elusive and they could not be assigned to individual core features. Hence, which proteins constitute which layers of the core, such as the palisade layer and the inner core wall, has remained enigmatic. Here we show, using a multi-modal cryo-electron microscopy (cryo-EM) approach in combination with AlphaFold molecular modeling, that trimers formed by the cleavage product of VACV protein A10 are the key component of the palisade layer. This allows us to place previously obtained descriptions of protein interactions within the core wall into perspective and to provide a detailed model of poxvirus core architecture. Importantly, we show that interactions within A10 trimers are likely generalizable over members of orthopox- and parapoxviruses.
Assuntos
Microscopia Crioeletrônica , Modelos Moleculares , Multimerização Proteica , Vaccinia virus/ultraestrutura , Vaccinia virus/química , Vaccinia virus/metabolismo , Poxviridae/ultraestrutura , Poxviridae/metabolismo , Poxviridae/química , Proteínas do Core Viral/química , Proteínas do Core Viral/ultraestrutura , Proteínas do Core Viral/metabolismo , HumanosRESUMO
The presence of carp edema virus (CEV) was confirmed in imported ornamental koi in Chiang Mai province, Thailand. The koi showed lethargy, loss of swimming activity, were lying at the bottom of the pond, and gasping at the water's surface. Some clinical signs such as skin hemorrhages and ulcers, swelling of the primary gill lamella, and necrosis of gill tissue, presented. Clinical examination showed co-infection by opportunistic pathogens including Dactylogyrus sp., Gyrodactylus sp. and Saprolegnia sp. on the skin and gills. Histopathologically, the gill of infected fish showed severe necrosis of epithelial cells and infiltrating of eosinophilic granular cells. Electron microscope examination detected few numbers of virions were present in the cytoplasm of gill tissue which showed an electron dense core with surface membranes worn by surface globular units. Molecular detection of CEV DNA from gill samples of fish was performed by polymerase chain reaction (PCR) and confirmed by nested-PCR. Phylogenetic analyses revealed that CEV isolate had 99.8% homology with the CEV isolated from South Korea (KY946715) and Germany (KY550420), and was assigned to genogroup IIa. In conclusion, this report confirmed the presence of CEV infection of koi Cyprinus carpio in Chiang Mai province, Thailand using pathological and molecular approaches.
Assuntos
Carpas/virologia , Doenças dos Peixes/virologia , Poxviridae , Animais , Biópsia , Genes Virais , Brânquias/patologia , Brânquias/virologia , Filogenia , Reação em Cadeia da Polimerase , Poxviridae/classificação , Poxviridae/genética , Poxviridae/ultraestrutura , TailândiaRESUMO
Proliferative gill disease (PGD) is an emerging problem in Norwegian culture of Atlantic salmon (Salmo salar). Parasites (Ichthyobodo spp.) and bacteria (Flexibacter/Flavobacterium) may cause PGD, but for most cases of PGD in farmed salmon in Norway, no specific pathogen has been identified as the causative agent. However, Neoparamoeba sp. and several bacteria and viruses have been associated with this disease. In the spring of 2006, a new poxvirus, salmon gill poxvirus (SGPV), was discovered on the gills of salmon suffering from PGD in fresh water in northern Norway. Later the same year, this virus was also found on gills of salmon at two marine sites in western Norway. All farms suffered high losses associated with the presence of this virus. In this study, we describe the entry and morphogenesis of the SGP virus in epithelial gill cells from Atlantic salmon. Intracellular mature virions (IMVs) are the only infective particles that seem to be produced. These are spread by cell lysis and by "budding" of virus packages, containing more that 100 IMVs, from the apical surface of infected cells. Entry of the IMVs appears to occur by attachment to microridges on the cell surface and fusion of the viral and cell membranes, delivering the cores into the cytoplasm. The morphogenesis starts with the emergence of crescents in viroplasm foci in perinuclear areas of infected cells. These crescents consist of two tightly apposed unit membranes (each 5 nm thick) that seem to be derived from membranes of the endoplasmic reticulum. The crescents develop into spheres, immature virions (IVs), that are 350 nm in diameter and surrounded by two unit membranes. The maturation of the IVs occurs by condensation of the core material and a change from spherical to boat-shaped particles, intracellular mature virions (IMVs), that are about 300 nm long. Hence, the IMVs from the SGP virus have a different morphology compared to other vertebrate poxviruses that are members of the subfamily Chordopoxvirinae, and they are more similar to members of subfamily Entomopoxvirinae, genus Alphaentomopoxvirus. However, it is premature to make a taxonomic assignment until the genome of the SGP virus has been sequenced, but morphogenesis clearly shows that this virus is a member of family Poxviridae.
Assuntos
Doenças dos Peixes/virologia , Brânquias/virologia , Poxviridae/fisiologia , Salmo salar/virologia , Montagem de Vírus , Internalização do Vírus , Animais , Citoplasma/ultraestrutura , Citoplasma/virologia , Células Epiteliais/diagnóstico por imagem , Células Epiteliais/virologia , Histocitoquímica , Microscopia Eletrônica de Transmissão , Dados de Sequência Molecular , Noruega , Poxviridae/ultraestrutura , UltrassonografiaRESUMO
BeAn 58058 virus (BAV) was isolated from an Oryzomis rodent in Brazil. BAV was shown to be antigenically related to another poxvirus also isolated in Brazil, the Cotia virus, but it remained ungrouped. Electron microscopy revealed that BAV has a typical poxvirus morphology. The Hind III DNA profile of BAV genome was similar with that of VV WR and Lister, but some differences in the profile were detected. We have also detected the presence of genes homologous to vaccinia virus (VV WR) genes in the genome of BAV. Genes related to vaccinia thymidine kinase (TK) gene and vaccinia growth factor (VGF) gene were found. The patterns of TK and VGF mRNA transcripts described for vaccinia virus infected cells were observed in BAV infected cells. Nucleotide sequence of BAV VGF homologous gene was similar to VV WR VGF sequences. This similarity was further seen when cross-hybridization of total genomes of BAV and VV was done. Polypeptide synthesis of BAV and vaccinia in infected cells also showed similar profiles. The genetic data was used to construct a phylogenetic tree where BAV and VV were placed at the same cluster. Based on our findings we propose that BAV is a vaccinia virus variant.
Assuntos
Poxviridae/genética , Sequência de Aminoácidos , Animais , Chlorocebus aethiops , DNA Viral/análise , Peptídeos e Proteínas de Sinalização Intercelular , Dados de Sequência Molecular , Peptídeos/genética , Filogenia , Poxviridae/classificação , Poxviridae/ultraestrutura , RNA Mensageiro/análise , Timidina Quinase/genética , Células VeroRESUMO
A case of cutaneous avian pox infection in a Hodgson's grandala (Grandala coelicolor) is described. The bird was emaciated and had nodules on the eyelids, bill, neck, legs, and toes. Eosinophilic intracytoplasmic inclusion bodies were visualized by light microscopy in epithelial cells of the cutaneous nodules. Electron microscopy revealed numerous pox virions in the inclusion bodies. This is the first report of cutaneous poxvirus infection in a Hodgson's grandala.
Assuntos
Doenças das Aves , Varíola Aviária/patologia , Dermatopatias/veterinária , Animais , Aves , Feminino , Microscopia Eletrônica , Poxviridae/isolamento & purificação , Poxviridae/ultraestrutura , Dermatopatias/patologia , Vírion/isolamento & purificação , Vírion/ultraestruturaRESUMO
A typical lesion of orf developed on the temple of a 61-year-old man who apparently contracted the infection indirectly. In addition to the classical histological features of epidermal hyperplasia, necrosis, ballooning and vesical formation, the lesion also showed florid pseudoneoplastic vascular proliferation and a prominent component of eosinophils in the dermal inflammation.
Assuntos
Ectima Contagioso/patologia , Face , Dermatopatias/diagnóstico , Animais , Diagnóstico Diferencial , Ectima Contagioso/etiologia , Granuloma Piogênico/diagnóstico , Humanos , Masculino , Microscopia Eletrônica , Pessoa de Meia-Idade , Poxviridae/ultraestrutura , OvinosRESUMO
In 1992 we began an investigation into incidents of unusual and mass mortalities of the common frog (Rana temporaria) in Britain which were being reported unsolicited to us in increasing numbers by members of the public. Investigations conducted at ten sites of unusual mortality resulted in two main disease syndromes being found: one characterized by skin ulceration and one characterized by systemic haemorrhages. However, frogs also were found with lesions common to both of these syndromes and microscopic skin lesions common to both syndromes were seen. The bacterium Aeromonas hydrophila, which has been described previously as causing similar lesions, was isolated significantly more frequently from haemorrhagic frogs than from those with skin ulceration only. However, as many of the latter were euthanased, this may have been due to differences in post mortem bacterial invasion. An iridovirus-like particle has been identified on electron microscopical examination of skin lesions from frogs with each syndrome and iridovirus-like inclusions have been detected in the livers of frogs with systemic haemorrhages. Also, an adenovirus-like particle has been cultured from one haemorrhagic frog. A poxvirus-like particle described previously from diseased frogs has now been found also in control animals and has been identified as a melanosome. Both the prevalence of the iridovirus-like particle and its association with lesions indicate that it may be implicated in the aetiology of the disease syndromes observed. Specifically, we hypothesize that primary iridovirus infection, with or without secondary infection with opportunistic pathogens such as A. hydrophila, may cause natural outbreaks of 'red-leg', a disease considered previously to be due to bacterial infection only.
Assuntos
Rana temporaria/crescimento & desenvolvimento , Pele/microbiologia , Pele/virologia , Adenoviridae/isolamento & purificação , Adenoviridae/ultraestrutura , Aeromonas hydrophila/isolamento & purificação , Animais , Eritema/microbiologia , Eritema/mortalidade , Eritema/veterinária , Feminino , Hemorragia/microbiologia , Hemorragia/mortalidade , Hemorragia/veterinária , Iridovirus/isolamento & purificação , Iridovirus/ultraestrutura , Masculino , Microscopia Eletrônica , Poxviridae/isolamento & purificação , Poxviridae/ultraestrutura , Pele/ultraestrutura , Úlcera Cutânea/microbiologia , Úlcera Cutânea/mortalidade , Úlcera Cutânea/veterinária , Análise de SobrevidaRESUMO
A poxvirus was isolated during the latter half of 1993 from a black-tailed deer (Odocoileus hemionus columbianus) that died of fulminant adenovirus infection in California (USA). The poxvirus was isolated from a pooled tissue homogenate, after repeated serial blind passages in primary black-tailed deer testicular cells. Based on electron microscopic examination of the virus, we observed morphologic features typical of the genus Orthopoxvirus, although definitive characterization was not done.
Assuntos
Cervos/virologia , Infecções por Poxviridae/veterinária , Poxviridae/isolamento & purificação , Infecções por Adenoviridae/complicações , Infecções por Adenoviridae/veterinária , Animais , Células Cultivadas , Masculino , Microscopia Eletrônica , Poxviridae/ultraestrutura , Infecções por Poxviridae/complicações , Infecções por Poxviridae/virologia , Inoculações Seriadas , Testículo/citologia , Testículo/virologia , Vírion/isolamento & purificação , Vírion/ultraestruturaRESUMO
Poxvirus was isolated from cutaneous nodules on two young Houbara bustards (Chlamydotis undulata) bred in captivity in Saudi Arabia. Birds were emaciated and presented nodules on tibiotarso-tarsometatarsal joints, toes, and the carpal joint. Diagnosis was confirmed by histopathology, virus isolation on inoculated chorioallantoic membranes of embryonated chicken's eggs, and electron microscopy. Progressive leg lesions were extensive and interfered with walking, significantly debilitating the birds. Successful excisions of these lesions were performed.
Assuntos
Doenças das Aves , Varíola Aviária/patologia , Dermatopatias/veterinária , Animais , Aves , Embrião de Galinha , Articulações/patologia , Microscopia Eletrônica , Poxviridae/crescimento & desenvolvimento , Poxviridae/isolamento & purificação , Poxviridae/ultraestrutura , Arábia Saudita , Pele/patologia , Dermatopatias/patologiaRESUMO
Polypeptide Ag35, a major early component of the vaccinia surface, is integrated into the formative viral lipoprotein tegument. To ascertain whether positioning of Ag35 is due to its general affinity for newly assembled viral membranes we created a recombinant A12 vector to express the vaccinia protein. The baculovirus system was chosen because intranuclear virions of this agent are likewise enclosed inside newly formed envelopes. Comparable infections of two insect cell lines established that more abundant synthesis occurred in High Five (H5) than in SF9 cells. We, therefore, used H5 cells for most experiments reported here. Combined analyses by PAGE, Western blotting, and immunocytology, using light and electron microscopy, revealed a dissemination of Ag35 throughout the cell. Higher concentrations were evident at the cell surface, nuclear perimeter, and within intranuclear virogenic stroma. The association with the virogenic stroma was of specific interest with respect to vaccinia development because it showed a similarity in the targeting of Ag35 toward intranuclear DNA-protein foci of baculovirus which are analogous to the vaccinia-specified cytoplasmic "factories." A further remarkable analogy concerns association of Ag35 with intranuclear baculovirus envelopes, revealing a propensity of Ag35 for nascent viral lipoprotein membranes.
Assuntos
Antígenos de Superfície/metabolismo , Antígenos Virais/metabolismo , Vírus de Insetos/metabolismo , Poxviridae/metabolismo , Proteínas Virais/metabolismo , Animais , Antígenos de Superfície/genética , Antígenos de Superfície/isolamento & purificação , Antígenos Virais/genética , Sequência de Bases , Transporte Biológico , Núcleo Celular/microbiologia , Células Cultivadas , Citoplasma/microbiologia , Imunofluorescência , Vetores Genéticos , Vírus de Insetos/crescimento & desenvolvimento , Vírus de Insetos/ultraestrutura , Dados de Sequência Molecular , Mariposas/citologia , Mariposas/ultraestrutura , Nucleopoliedrovírus/genética , Poxviridae/crescimento & desenvolvimento , Poxviridae/ultraestrutura , Biossíntese de Proteínas , Proteínas Recombinantes/isolamento & purificação , Proteínas Recombinantes/metabolismo , Proteínas Virais/genética , Proteínas Virais/isolamento & purificação , Vírion/crescimento & desenvolvimento , Vírion/ultraestruturaRESUMO
Entomopoxvirus virions are frequently contained within crystalline occlusion bodies, which are composed of primarily a single protein, spheroidin, which is analogous to the polyhedrin protein of baculovirus. The spheroidin gene of Amsacta moorei entomopoxvirus was identified following the microsequencing of polypeptides generated from cyanogen bromide treatment of spheroidin and the subsequent synthesis of oligonucleotide hybridization probes. DNA sequencing of a 6.8-kb region of DNA containing the spheroidin gene showed that the spheroidin protein is derived from a 3.0-kb open reading frame potentially encoding a protein of 115 kDa. Three copies of the heptanucleotide, TTTTTNT, a sequence associated with early gene transcription in the vertebrate poxviruses, and four in-frame translational termination signals were found within 60 bp upstream of the putative spheroidin gene promoter (TAAATG). The spheroidin gene promoter region contains the sequence TAAATG, which is found in many late promoters of the vertebrate poxviruses and which serves as the site of transcriptional initiation, as shown by primer extension. Primer extension experiments also showed that spheroidin gene transcripts contain 5' poly(A) sequences typical of vertebrate poxvirus late transcripts. The 92 bases upstream of the initiating TAAATG are unusually A + T rich and contain only 7 G or C residues. An analysis of open reading frames around the spheroidin gene suggests that the colinear core of "essential genes" typical of the vertebrate poxviruses is absent in A. moorei entomopoxvirus.
Assuntos
DNA Viral/genética , Genoma Viral , Vírus de Insetos/genética , Fases de Leitura Aberta , Poxviridae/genética , Vaccinia virus/genética , Proteínas Virais/genética , Sequência de Aminoácidos , Animais , Composição de Bases , Sequência de Bases , DNA Viral/isolamento & purificação , Vírus de Insetos/ultraestrutura , Insetos , Microscopia Eletrônica de Varredura , Dados de Sequência Molecular , Oligodesoxirribonucleotídeos , Poxviridae/ultraestrutura , Biossíntese de Proteínas , Mapeamento por Restrição , Homologia de Sequência do Ácido Nucleico , Transcrição Gênica , Proteínas Estruturais ViraisAssuntos
Doenças dos Bovinos/microbiologia , Papiloma/veterinária , Papillomaviridae/isolamento & purificação , Poxviridae/isolamento & purificação , Neoplasias Cutâneas/veterinária , Animais , Bovinos , Mandíbula , Papiloma/microbiologia , Papillomaviridae/ultraestrutura , Poxviridae/ultraestrutura , Neoplasias Cutâneas/microbiologia , Vírion/isolamento & purificação , Vírion/ultraestruturaRESUMO
Cutaneous tumour-like growths were observed on the face and other areas of the body surface of young Chilean flamingos. In the cells of these lesions, avian pox-specific cytoplasmic inclusion bodies were observed by light microscopy and virus particles were detected under an electron-microscope. It was diagnosed as avian pox.
Assuntos
Doenças das Aves/microbiologia , Infecções por Poxviridae/veterinária , Dermatopatias/veterinária , Animais , Doenças das Aves/patologia , Aves , Microscopia Eletrônica , Poxviridae/ultraestruturaRESUMO
Virus characterization studies were performed to meliorate the taxonomic status of three currently unclassified, serologically related viruses: Tanapox virus (causes vesicular skin lesions in humans), Yaba-like disease (YLD) virus (causes vesicular skin lesions in monkeys), and Yaba monkey tumor virus (YMTV, causes epidermal histiocytoma). These studies included (1) microscopic observations of Tanapox virus cytopathic effect and morphogenesis during its 6-day cytolytic-type growth at 35 degrees in CV-1 monkey kidney cells; (2) resolution of Tanapox virion proteins by sodium dodecyl sulfate-polyacrylamide gel electrophoresis of nonenveloped and double-enveloped virus particles purified by velocity sedimentation in sucrose and CsCl density gradients; and (3) restriction endonuclease DNA comparison of the three viruses. DNA analysis showed that six recent Tanapox virus isolates from patients in Zaire, Africa, were identical to Tanapox virus, Kenya strain, from 1957 from a patient in the Tana River Valley. In addition, BamHI, MluI, and PstI cleavage sites mapped on the DNA of Kenya Tanapox virus, and PstI sites mapped on DNA of YLD virus differentiated YLD and Tanapox viruses as separate strains. On the other hand, YMTV shared few restriction endonuclease sites with Tanapox and YLD viruses, although all three cross-hybridized extensively. These studies along with published viral characteristics, support the formation of a new poxvirus genus: the suggested name is Yatapoxvirus, and the genus currently comprises two species, Tanapox virus and YMTV.
Assuntos
Poxviridae/análise , Proteínas Virais/análise , Células Cultivadas , Efeito Citopatogênico Viral , DNA Viral/genética , Humanos , Técnicas In Vitro , Microscopia Eletrônica , Peso Molecular , Poxviridae/classificação , Poxviridae/genética , Poxviridae/ultraestrutura , Mapeamento por Restrição , Replicação ViralRESUMO
Two strains of papular stomatitis (PS) virus, 1 of milker's nodules (MN) virus and 1 of contagious ecthyma (CE) virus possessed 2 distinct external structures when examined by electron microscopy. The innermost, designated coat was closely apposed to the tubular surface, whereas the outer envelope loosely surrounded the virion. When convalescent sera from cattle infected with PS virus were used for immunoelectron microscopy, antibody reacted with coats and envelopes of the PS virus strains, but only with coats of MN and CE viruses. Convalescent sera from cattle infected with PS or MN virus contained complement-dependent antibodies cytolytic to cells infected with the homologous virus. In an indirect immunofluorescence test, the sera reacted with homologous strains to higher titers than with heterologous strains.
Assuntos
Antígenos Virais/imunologia , Doenças dos Bovinos/microbiologia , Epitopos , Infecções por Poxviridae/veterinária , Poxviridae/imunologia , Estomatite/veterinária , Animais , Bovinos , Doenças dos Bovinos/imunologia , Imunofluorescência , Soros Imunes/imunologia , Microscopia Eletrônica/métodos , Poxviridae/ultraestrutura , Infecções por Poxviridae/imunologia , Infecções por Poxviridae/microbiologia , Estomatite/imunologia , Estomatite/microbiologiaAssuntos
Vírus da Varíola das Aves Domésticas/ultraestrutura , Varíola Aviária/microbiologia , Poxviridae/ultraestrutura , Animais , Aves , Efeito Citopatogênico Viral , Vírus da Varíola das Aves Domésticas/imunologia , Vírus da Varíola das Aves Domésticas/patogenicidade , Masculino , Microscopia EletrônicaRESUMO
Microscopic lesions in cattle infected with the virus of the Neethling form of lumpy skin disease comprised a granulomatous reaction in the dermis and hypodermis which extended to the surrounding tissue. During the early stages of the lesions a vasculitis and lymphangitis with concomitant thrombosis and infarction resulted in necrosis and oedema. A hallmark of the acute to subacute stages of the lesions was the presence of intracytoplasmic eosinophilic inclusions in various cell types. The inclusions consisted of the viroplasm which was identified as aggregates of electron-dense, finely granular to fibrillar deposits in which membrane-enclosed virions and occasional groups of tubular structures were observed. Various cytopathogenic changes were observed in cells exhibiting viral proliferation. The morphogenesis of the virions is discussed in relation to the cytopathogenic changes.
Assuntos
Doença Nodular Cutânea/patologia , Vírus da Doença Nodular Cutânea/ultraestrutura , Poxviridae/ultraestrutura , Pele/patologia , Animais , Bovinos , Vírion/ultraestruturaAssuntos
Viroses/diagnóstico , Vírus/ultraestrutura , Animais , Anticorpos Antivirais/análise , Encéfalo/microbiologia , Fezes/microbiologia , Hepatite Viral Humana/diagnóstico , Humanos , Fígado/microbiologia , Microscopia Eletrônica/métodos , Nasofaringe/microbiologia , Neoplasias/microbiologia , Paramyxoviridae/ultraestrutura , Poxviridae/ultraestrutura , Retroviridae/ultraestrutura , Dermatopatias/microbiologia , Coloração e Rotulagem , Urina/microbiologia , Vírus/isolamento & purificaçãoRESUMO
The growth of poxvirions in cell culture is considered a teratogen screening test, since this virus has a rapid, simple morphogenetic pathway that is dependent upon cell proliferation. Vaccinia WR-infected BSC 40 monolayers were exposed to 42 known teratogens and 9 nonteratogens at dosages from 1 microM to 100 mM. After 24 h of infection, the number of functional virions was determined by plaque assay. Thirty-three of the 42 teratogens inhibited the virus, 3 teratogens stimulated the virus, and 6 teratogens were false-negatives. Eight of the 9 nonteratogens had no effect on virus proliferation at dosages as high as 600 times the lowest reported teratogenic dosage. The number of new virions could be directly related to the concentration of the teratogen in vitro, thus allowing each compound to be characterized by an RD50. The RD50 dosage in milligrams per liter was 98% correlated with the lowest reported teratogenic dose in vivo in milligrams per kilogram. In sum, vaccinia-infected cells have an easily identifiable endpoint, plaque-forming units, which may be an accurate prognosticator of teratogenesis.
Assuntos
Poxviridae/efeitos dos fármacos , Teratogênicos/toxicidade , Poxviridae/ultraestrutura , Vaccinia virus/efeitos dos fármacos , Ensaio de Placa ViralRESUMO
The poxvirus Cotia was studied by electron microscopy and by serological and biochemical analyses. Thin-sectioned preparations of infected Vero cells indicated that Cotia virus morphogenesis was similar to other mammalian poxviruses; unique filamentous structures and inclusion matrices were apparent in the cytoplasm. Complement fixation tests that included purified Cotia virions showed a reciprocal cross-reaction with rabbit myxoma virus and no cross-reaction with vaccinia virus. Serological results coupled with gradient polyacrylamide gel electropherograms of the structural proteins of purified Cotia, vaccinia, myxoma and fibroma viruses suggested that Cotia virus was similar to the latter two viruses. Agarose gel electropherograms of cleavage fragments of each of these virus DNAs digested with three separate restriction endonucleases showed that each of these viruses had a unique DNA gel profile.