1.
Biosci Biotechnol Biochem
; 76(3): 594-7, 2012.
Artigo
em Inglês
| MEDLINE
| ID: mdl-22451407
RESUMO
There are difficulties in detecting and separating rice prolamin polypeptides by 2D-PAGE analysis because prolamin polypeptides are insoluble, and the amino acid sequences show high homology among them. In this study, we improved the prolamin extraction method and the 2D-PAGE procedure, and succeeded in separating prolamin polypeptide species by 2D-PAGE and in identifying major prolamin polypeptide sequences.