RESUMO
Entry of bacteria into host cells is an important virulence mechanism. Through peptidoglycan recognition, the nucleotide-binding oligomerization domain (NOD) proteins NOD1 and NOD2 enable detection of intracellular bacteria and promote their clearance through initiation of a pro-inflammatory transcriptional programme and other host defence pathways, including autophagy. Recent findings have expanded the scope of the cellular compartments monitored by NOD1 and NOD2 and have elucidated the signalling pathways that are triggered downstream of NOD activation. In vivo, NOD1 and NOD2 have complex roles, both during bacterial infection and at homeostasis. The association of alleles that encode constitutively active or constitutively inactive forms of NOD2 with different diseases highlights this complexity and indicates that a balanced level of NOD signalling is crucial for the maintenance of immune homeostasis.
Assuntos
Inflamação/imunologia , Proteínas Adaptadoras de Sinalização NOD/imunologia , Imunidade Adaptativa , Animais , Autofagia , Infecções Bacterianas/imunologia , Humanos , Imunidade Inata , Intestinos/imunologia , Neoplasias/imunologia , Proteínas Adaptadoras de Sinalização NOD/química , Proteínas Adaptadoras de Sinalização NOD/fisiologia , Peptidoglicano/imunologia , Transdução de SinaisRESUMO
In addition to reduced-intensity conditioning, which has expanded the eligibility for hematopoietic cell transplantation (HCT) to older patients, increased availability of alternative donors, including HLA-mismatched unrelated donors, has increased access to allogeneic HCT for more patients. However, acute graft-versus-host disease (GVHD) remains a lethal complication, even in HLA-matched donor-recipient pairs. The pathophysiology of GVHD depends on aspects of adaptive immunity and interactions between donor T-cells and host dendritic cells (DCs). Recent work has revealed that the role of other immune cells and endothelial cells and components of the innate immune response are also important. Tissue damage caused by the conditioning regimen leads to the release of exogenous and endogenous "danger signals". Exogenous danger signals called pathogen-associated molecular patterns and endogenous noninfectious molecules known as damage-associated molecular patterns (DAMPs) are responsible for initiating or amplifying acute GVHD by enhancing DC maturation and alloreactive T-cell responses. A significant association of innate immune receptor polymorphisms with outcomes, including GVHD severity, was observed in patients receiving allogeneic HCT. Understanding of the role of innate immunity in acute GVHD might offer new therapeutic approaches.
Assuntos
Doença Enxerto-Hospedeiro/imunologia , Transplante de Células-Tronco Hematopoéticas , Imunidade Inata/genética , Imunidade Inata/imunologia , Isoantígenos/imunologia , Doença Aguda , Trifosfato de Adenosina/fisiologia , Aloenxertos , Células Dendríticas/imunologia , Doença Enxerto-Hospedeiro/genética , Doença Enxerto-Hospedeiro/prevenção & controle , Doença Enxerto-Hospedeiro/terapia , Proteína HMGB1/fisiologia , Proteínas de Choque Térmico/fisiologia , Heparitina Sulfato/fisiologia , Humanos , Ácido Hialurônico/fisiologia , Terapia de Alvo Molecular , Proteínas Adaptadoras de Sinalização NOD/fisiologia , Polimorfismo Genético , Proteínas S100/fisiologia , Transdução de Sinais , Linfócitos T/imunologia , Receptores Toll-Like/genética , Receptores Toll-Like/imunologia , Condicionamento Pré-Transplante/efeitos adversosRESUMO
Intestinal inflammation is characterized by epithelial disruption, leading to loss of barrier function and the recruitment of immune cells, including neutrophils. Although the mechanisms are not yet completely understood, interactions between environmental and immunological factors are thought to be critical in the initiation and progression of intestinal inflammation. In recent years, it has become apparent that the di/tripeptide transporter PepT1 may play an important role in the pathogenesis of such inflammation. In healthy individuals, PepT1 is primarily expressed in the small intestine and transports di/tripeptides for metabolic purposes. However, during chronic inflammation such as that associated with inflammatory bowel disease, PepT1 expression is upregulated in the colon, wherein the protein is normally expressed either minimally or not at all. Several recent studies have shown that PepT1 binds to and transports various bacterial di/tripeptides into colon cells, leading to activation of downstream proinflammatory responses via peptide interactions with innate immune receptors. In the present review, we examine the relationship between colonic PepT1-mediated peptide transport in the colon and activation of innate immune responses during disease. It is important to understand the mechanisms of PepT1 action during chronic intestinal inflammation to develop future therapies addressing inappropriate immune activation in the colon.
Assuntos
Gastroenterite/etiologia , Doenças Inflamatórias Intestinais/etiologia , Simportadores/fisiologia , Animais , Neoplasias Colorretais/fisiopatologia , Gastroenterite/tratamento farmacológico , Humanos , Doenças Inflamatórias Intestinais/genética , Doenças Inflamatórias Intestinais/fisiopatologia , Proteínas Adaptadoras de Sinalização NOD/fisiologia , Oligopeptídeos/metabolismo , Transportador 1 de Peptídeos , Simportadores/genéticaRESUMO
The well-being of the intestine and its host requires that this organ execute its complex function amid colonization by a large and diverse microbial community referred to as the gut microbiota. A myriad of interacting mechanisms of mucosal immunity permit the gut to corral the microbiota in such a way as to maximize the benefits and to minimize the danger of living in close proximity to this large microbial biomass. Toll-like receptors and Nod-like receptors, collectively referred to as pattern recognition receptors (PRRs), recognize a variety of microbial components and, hence, play a central role in governing the interface between host and microbiota. This review examines mechanisms by which PRR-microbiota interactions are regulated so as to allow activation of host defense when necessary while preventing excessive inflammation, which can have a myriad of negative consequences for the host. Analysis of published studies performed in human subjects and a variety of murine disease models reveals the central theme that PRRs play a key role in maintaining a healthful stable relationship between the intestine and its microbiota. In contrast, although select genetic ablations of PRR signaling may protect against some chronic diseases, the overriding theme of studies performed to date is that perturbations of PRR-microbiota interactions are more likely to promote disease states associated with inflammation.
Assuntos
Trato Gastrointestinal/microbiologia , Metagenoma/fisiologia , Receptores Toll-Like/fisiologia , Animais , Colite/microbiologia , Colite/fisiopatologia , Escherichia coli/fisiologia , Neoplasias Gastrointestinais/fisiopatologia , Trato Gastrointestinal/imunologia , Homeostase/fisiologia , Humanos , Doenças Metabólicas/microbiologia , Doenças Metabólicas/fisiopatologia , Proteínas Adaptadoras de Sinalização NOD/fisiologia , Receptores de Reconhecimento de Padrão/fisiologia , Transdução de Sinais/fisiologiaRESUMO
Candida albicans is a common fungal pathogen of humans that colonizes the skin and mucosal surfaces of most healthy individuals. Until recently, little was known about the mechanisms by which mucosal antifungal defences tolerate colonizing C. albicans but react strongly when hyphae of the same microorganism attempt to invade tissue. In this Review, we describe the properties of yeast cells and hyphae that are relevant to their interaction with the host, and the immunological mechanisms that differentially recognize colonizing versus invading C. albicans.
Assuntos
Candida albicans/fisiologia , Candidíase/microbiologia , Animais , Candida albicans/crescimento & desenvolvimento , Candidíase/imunologia , Candidíase/fisiopatologia , Parede Celular/fisiologia , Proteínas Quinases Dependentes de AMP Cíclico/fisiologia , Humanos , Hifas/crescimento & desenvolvimento , Hifas/fisiologia , Lectinas Tipo C/fisiologia , Morfogênese/fisiologia , Proteínas Adaptadoras de Sinalização NOD/fisiologia , Transdução de Sinais/fisiologia , Pele/imunologia , Pele/microbiologia , Receptores Toll-Like/fisiologiaRESUMO
Stimulation of almost all mammalian cell types leads to the release of cellular ATP and autocrine feedback through a diverse array of purinergic receptors. Depending on the types of purinergic receptors that are involved, autocrine signalling can promote or inhibit cell activation and fine-tune functional responses. Recent work has shown that autocrine signalling is an important checkpoint in immune cell activation and allows immune cells to adjust their functional responses based on the extracellular cues provided by their environment. This Review focuses on the roles of autocrine purinergic signalling in the regulation of both innate and adaptive immune responses and discusses the potential of targeting purinergic receptors for treating immune-mediated disease.
Assuntos
Imunidade Adaptativa/fisiologia , Trifosfato de Adenosina/fisiologia , Comunicação Autócrina/fisiologia , Imunidade Inata/fisiologia , Receptores Purinérgicos/fisiologia , Adenosina/metabolismo , Trifosfato de Adenosina/metabolismo , Animais , Quimiotaxia/fisiologia , Humanos , Tolerância Imunológica/fisiologia , Leucócitos/imunologia , Leucócitos/metabolismo , Ativação Linfocitária/fisiologia , Linfócitos/imunologia , Linfócitos/metabolismo , Macrófagos/fisiologia , Proteínas Adaptadoras de Sinalização NOD/fisiologia , Proteínas de Transporte de Nucleosídeos/fisiologia , Pirofosfatases/fisiologia , Transdução de Sinais/imunologia , Receptores Toll-Like/fisiologiaRESUMO
BACKGROUND: Nucleotide-binding oligomerization domain (NOD)-like receptors (NLRs) are newly discovered cytosolic receptors belonging to the pattern-recognition receptor family. They detect various pathogen-associated molecular patterns, triggering an immune response. The knowledge about these receptors, and their role in health and disease, is limited. The aim of the present study was to characterize the expression of NOD1, NOD2, and NALP3 in the human upper airways. METHODS: Surgical samples were obtained from patients with tonsillar disease (n = 151), hypertrophic adenoids (n = 9), and nasal polyposis (n = 24). Nasal biopsies were obtained from healthy volunteers (n = 10). The expression of NOD1, NOD2, and NALP3 was analyzed using real-time PCR and immunohistochemistry. RESULTS: Expression of NOD1, NOD2, and NALP3 mRNA and protein were seen in all tissue specimens. The NLR mRNA was found to be higher in nasal polyps than in normal nasal mucosa, and local steroid treatment reduced the NLR expression in polyps. In contrast, tonsillar infection with Streptococcus pyogenes or Haemophilus influenzae did not affect the NLR expression. CONCLUSIONS: The present study demonstrates the presence of NLRs in several upper airway tissues and highlights a potential role of NLRs in chronic rhinosinusitis with polyps.
Assuntos
Pólipos Nasais/etiologia , Proteínas Adaptadoras de Sinalização NOD/fisiologia , Sistema Respiratório/química , Adolescente , Adulto , Idoso , Idoso de 80 Anos ou mais , Proteínas de Transporte/análise , Proteínas de Transporte/genética , Estudos de Casos e Controles , Criança , Pré-Escolar , Feminino , Humanos , Imunidade Inata , Masculino , Pessoa de Meia-Idade , Proteína 3 que Contém Domínio de Pirina da Família NLR , Pólipos Nasais/química , Proteínas Adaptadoras de Sinalização NOD/análise , Proteínas Adaptadoras de Sinalização NOD/genética , Proteína Adaptadora de Sinalização NOD1/análise , Proteína Adaptadora de Sinalização NOD1/genética , Proteína Adaptadora de Sinalização NOD2/análise , Proteína Adaptadora de Sinalização NOD2/genética , RNA Mensageiro/análise , Distribuição Tecidual , Adulto JovemRESUMO
Helicobacter pylori colonize the gastric epithelial, most infected people are asymptomatic, 10 to 20% develop atrophic gastritis, peptic ulcer and less than 3% gastric cancer. These diseases are determined by the relationship between virulence factors of bacteria, host factors such as, genetic predisposition, and immune response. The innate immune response mainly represented by Toll-like receptors and Nod-like receptors that recognize their specific ligands, activate transcription factors as NF-kB, AP-1, CREB-1, inducing production of inflammatory cytokines such as IL -8, IL-12, IL-6, IL-1ß, IL-18, TNF-α and IL-10. Chronic inflammation promotes gastric morphological changes, prevents apoptosis and allows angiogenesis generating neoplasic lesions and cancer. The aim of this review is to analyze the mechanisms proposed to date of the innate and adaptative immune response involved in H. pylori infection; remarking the mechanisms related in the elimination or persistence.
Assuntos
Citocinas/fisiologia , Gastrite/imunologia , Infecções por Helicobacter/imunologia , Helicobacter pylori/imunologia , Proteínas Adaptadoras de Sinalização NOD/fisiologia , Lesões Pré-Cancerosas/imunologia , Receptores Toll-Like/fisiologia , Vacinas Bacterianas , Ilhas Genômicas , Infecções por Helicobacter/prevenção & controle , Helicobacter pylori/genética , Helicobacter pylori/patogenicidade , Interações Hospedeiro-Patógeno/imunologia , Humanos , Imunidade InataRESUMO
Helicobacter pylori coloniza el epitelio gástrico y la mayoría de las personas infectadas es asintomática, de 10 al 20 por ciento desarrolla gastritis atrófica, úlcera péptica, y menos de 3 por ciento genera cáncer gástrico. Estas patologías están determinadas por la relación entre los factores de virulencia de la bacteria y los factores del hospedero como predisposición genética y respuesta inmune. La inmunidad innata, representada principalmente por los receptores tipo Toll y tipo Nod, reconocen a sus ligandos específicos y activan factores de transcripción como NF-kB, AP-1, CREB-1, induciendo la producción de citocinas inflamatorias como IL-8, IL-12, IL-6, IL-1β, IL-18 y TNF-α, e IL-10. La inflamación crónica favorece los cambios de morfología gástrica, evita la apoptosis y favorece la angiogénesis, ocasionando lesiones neoplásicas y cáncer. El objetivo de esta revisión es analizar los mecanismos propuestos a la fecha de la respuesta inmune innata y adaptativa, involucrados en la infección por H. pylori, y se puntualiza en los mecanismos de eliminación o persistencia de la infección.
Helicobacter pylori colonize the gastric epithelial, most infected people are asymptomatic, 10 to 20 percent develop atrophic gastritis, peptic ulcer and less than 3 percent gastric cancer. These diseases are determined by the relationship between virulence factors of bacteria, host factors such as, genetic predisposition, and immune response. The innate immune response mainly represented by Toll-like receptors and Nod-like receptors that recognize their specific ligands, activate transcription factors as NF-kB, AP-1, CREB-1, inducing production of inflammatory cytokines such as IL -8, IL-12, IL-6, IL-1β, IL-18, TNF-α and IL-10. Chronic inflammation promotes gastric morphological changes, prevents apoptosis and allows angiogenesis generating neoplasic lesions and cancer. The aim of this review is to analyze the mechanisms proposed to date of the innate and adaptative immune response involved in H. pylori infection; remarking the mechanisms related in the elimination or persistence.
Assuntos
Humanos , Citocinas/fisiologia , Gastrite/imunologia , Infecções por Helicobacter/imunologia , Helicobacter pylori/imunologia , Proteínas Adaptadoras de Sinalização NOD/fisiologia , Lesões Pré-Cancerosas/imunologia , Receptores Toll-Like/fisiologia , Vacinas Bacterianas , Ilhas Genômicas , Infecções por Helicobacter/prevenção & controle , Helicobacter pylori/genética , Helicobacter pylori/patogenicidade , Interações Hospedeiro-Patógeno/imunologia , Imunidade InataAssuntos
Aterosclerose/complicações , Tolerância Imunológica/fisiologia , Imunidade Inata/fisiologia , Obesidade/complicações , Periodontite/imunologia , Porphyromonas gingivalis/imunologia , Animais , Aterosclerose/imunologia , Endotélio Vascular/microbiologia , Humanos , Inflamação/imunologia , Macrófagos/fisiologia , Proteínas Adaptadoras de Sinalização NOD/fisiologia , Obesidade/imunologia , Periodontite/complicações , Periodontite/microbiologia , Receptores Toll-Like/fisiologiaRESUMO
Chronic inflammation is known to promote the development of many chronic diseases. Pattern recognition receptors (PRRs), Toll-like receptors (TLRs), and nucleotide-binding oligomerization domain proteins (NODs) mediate both infection-induced inflammation and sterile inflammation by recognizing pathogen- associated molecular patterns and endogenous molecules, respectively. PRR-mediated inflammation is an important determinant in altering the risk of many chronic diseases. Saturated fatty acids (SFAs) can activate PRRs, leading to enhanced expression of pro-inflammatory target gene products. However, n-3 polyunsaturated fatty acids (PUFAs) inhibit agonist-induced activation of PRRs. These results suggest that SFAs and n-3 PUFAs can reciprocally modulate PRR-mediated inflammation, and that PRRs and their downstream signaling components are molecular targets for dietary strategies to reduce chronic inflammation and subsequent risk of chronic diseases. This advancement in knowledge provides a new paradigm for understanding the mechanism by which different dietary fatty acids modify risk of chronic diseases including insulin resistance, atherosclerosis, and cancer.
Assuntos
Gorduras na Dieta , Ácidos Graxos , Inflamação/fisiopatologia , Receptores de Reconhecimento de Padrão/fisiologia , Animais , Doença Crônica/prevenção & controle , Ácidos Graxos/metabolismo , Ácidos Graxos/fisiologia , Ácidos Graxos Ômega-3/metabolismo , Ácidos Graxos Ômega-3/fisiologia , Humanos , Proteínas Adaptadoras de Sinalização NOD/agonistas , Proteínas Adaptadoras de Sinalização NOD/antagonistas & inibidores , Proteínas Adaptadoras de Sinalização NOD/fisiologia , Receptores de Reconhecimento de Padrão/agonistas , Receptores de Reconhecimento de Padrão/antagonistas & inibidores , Fatores de Risco , Receptores Toll-Like/agonistas , Receptores Toll-Like/antagonistas & inibidores , Receptores Toll-Like/fisiologiaRESUMO
Cell death and innate immunity are ancient evolutionary conserved processes that utilize a dazzling number of related molecular effectors and parallel signal transduction mechanisms. The investigation of the molecular mechanisms linking the sensing of a danger signal (pathogens or tissue damage) to the induction of an inflammatory response has witnessed a renaissance in the last few years. This was initiated by the identification of pattern recognition receptors (PRRs), including Toll-like receptors (TLRs) and more recently cytosolic Nod-like receptors (NLRs), that brought innate immunity to center stage and opened the field to the study of signal transduction pathways, adaptors and central effectors linked to PRRs. This led to the characterization of the inflammasome, a macromolecular complex, scaffolded by NLRs, that recruits and activates inflammatory caspases, which are essential effectors in inflammation and cell death responses. In this review, we describe the molecular pathways of cell death and innate immunity with a focus on recent advancements in both fields and an emphasis on the striking analogies between NLR innate immunity and mitochondrial apoptosis pathways.
Assuntos
Apoptose/fisiologia , Imunidade Inata/fisiologia , Receptores de Reconhecimento de Padrão/fisiologia , Animais , Fenômenos Fisiológicos Bacterianos , Humanos , Inflamação/fisiopatologia , Neoplasias/patologia , Proteínas Adaptadoras de Sinalização NOD/fisiologia , Transdução de Sinais/fisiologia , Linfócitos T/fisiologiaRESUMO
Crohn's disease and ulcerative colitis are idiopathic inflammatory bowel disorders. In this paper, we discuss how environmental factors (eg, geography, cigarette smoking, sanitation and hygiene), infectious microbes, ethnic origin, genetic susceptibility, and a dysregulated immune system can result in mucosal inflammation. After describing the symbiotic interaction of the commensal microbiota with the host, oral tolerance, epithelial barrier function, antigen recognition, and immunoregulation by the innate and adaptive immune system, we examine the initiating and perpetuating events of mucosal inflammation. We pay special attention to pattern-recognition receptors, such as toll-like receptors and nucleotide-binding-oligomerisation-domains (NOD), NOD-like receptors and their mutual interaction on epithelial cells and antigen-presenting cells. We also discuss the important role of dendritic cells in directing tolerance and immunity by modulation of subpopulations of effector T cells, regulatory T cells, Th17 cells, natural killer T cells, natural killer cells, and monocyte-macrophages in mucosal inflammation. Implications for novel therapies, which are discussed in detail in the second paper in this Series, are covered briefly.
Assuntos
Exposição Ambiental/efeitos adversos , Predisposição Genética para Doença , Sistema Imunitário/fisiologia , Doenças Inflamatórias Intestinais , Proteínas Adaptadoras de Sinalização NOD/fisiologia , Humanos , Doenças Inflamatórias Intestinais/etiologia , Doenças Inflamatórias Intestinais/genética , Doenças Inflamatórias Intestinais/imunologia , Intestinos/imunologia , Cariotipagem , Estilo de Vida , Proteínas Adaptadoras de Sinalização NOD/imunologia , Prevalência , Fatores de RiscoRESUMO
NOD-like receptors are cytosolic proteins that contain a central nucleotide-binding oligomerization domain (NACHT), an N-terminal effector-binding domain and C-terminal leucine-rich repeats (LRRs). NOD-like receptors have been implicated as ancient cellular sentinels mediating protective immune responses against intracellular pathogens. Recent studies have described the genetic association of polymorphisms in NOD-like receptor genes with complex chronic inflammatory barrier diseases, such as Crohn's disease and asthma and with rare auto-inflammatory syndromes including familial cold urticaria, Muckle-Wells syndrome and Blau syndrome. Whereas genetic variability in NLRs may have been an important element to provide plasticity to antigen recognition and host defense in the past, recent changes in the lifestyle of industrialized societies (e.g. hygiene ("cold-chain-hypothesis"), nutrition, or antibiotics) may have turned ancient genetic variability into disease-causing mutations. The review focuses on NLR function in the molecular pathophysiology of human inflammatory disorders.
Assuntos
Proteínas Adaptadoras de Sinalização NOD/fisiologia , Receptores de Superfície Celular/fisiologia , Receptores de Reconhecimento de Padrão/fisiologia , Doença de Crohn/metabolismo , Doença de Crohn/fisiopatologia , Doenças Genéticas Inatas/metabolismo , Doenças Genéticas Inatas/fisiopatologia , Humanos , Imunidade Inata , Inflamação/fisiopatologiaRESUMO
Defensins and cathelicidins are prevalent and essential gastrointestinal cationic antimicrobial peptides (CAPs). However, these defensive peptides are not infallible because certain enteropathogens can overcome their protective function. Furthermore, impaired defensin synthesis has been linked to the occurrence of Crohn's disease (CD), a chronic inflammatory bowel disease. Recently, defective bacterial sensing through NOD1 and NOD2 has been related to reduced defensin production, CD predisposition and susceptibility to enteric infection. Hence, we propose that microbial sensors at the gut interface monitor the levels of these effector peptides, which might function as "danger" signals to confer tolerance and alert immunocytes. Further work is required to clarify how gastrointestinal CAPs are regulated and to assess their role in maintaining epithelial homeostasis and triggering adaptive immunity.