The Potential Virulence Factors of Providencia stuartii: Motility, Adherence, and Invasion.

Providencia stuartii is the most common Providencia species capable of causing human infections. Currently P. stuartii is involved in high incidence of urinary tract infections in catheterized patients. The ability of bacteria to swarm on semisolid (viscous) surfaces and adhere to and invade host cells determines the specificity of the disease pathogenesis and its therapy. In the present study we demonstrated morphological changes of P. stuartii NK cells during migration on the viscous medium and discussed adhesive and invasive properties utilizing the HeLa-M cell line as a host model. To visualize the interaction of P. stuartii NK bacterial cells with eukaryotic cells in vitro scanning electron and confocal microscopy were performed. We found that bacteria P. stuartii NK are able to adhere to and invade HeLa-M epithelial cells and these properties depend on the age of bacterial culture. Also, to invade the host cells the infectious dose of the bacteria is essential. The microphotographs indicate that after incubation of bacterial P. stuartii NK cells together with epithelial cells the bacterial cells both were adhered onto and invaded into the host cells.

Enterocyte-like Caco-2 cells as a model for in vitro studies of diarrhoeagenic Providencia alcalifaciens invasion.

The entry of Providencia alcalifaciens into the enterocyte-like cell line Caco-2 compared to HEp-2 was studied. Of the 22 P. alcalifaciens strains, 13 and 21 were invasive for Caco-2 and HEp-2 cells, respectively. In contrast to HEp-2 cells, P. alcalifaciens was internalised by Caco-2 cells via receptor-mediated endocytosis. Tyrosine kinases play an important role in P. alcalifaciens uptake, also microfilaments and microtubules are engaged in this process. Inhibition of endosome acidification by ammonium chloride did not seem to have any significant effect on P. alcalifaciens invasion. Similarly to Shigella flexnerii, the invasion of Caco-2 cells by these bacteria occurred more effectively through the basolateral pole than through the apical surface of these cells. Plasmid DNA analysis showed the presence of plasmids of 5-172 kb in 13 strains regardless of their invasive ability. The presence of extracellular bacterial protein, most likely a kind of an invasin, is required for the invasion of Caco-2 and HEp-2 cells.

Demonstration and characterization of manganese superoxide dismutase of Providencia alcalifaciens.

Superoxide dismutases convert superoxide anions to molecular oxygen and hydrogen peroxide. These enzymes constitute one of the major defense mechanisms of cells against oxidative stress and play a role in the pathogenesis of certain invasive bacteria. In this study, we reported for the first time here that Providencia alcalifaciens, a member of the family Enterobacteriaceae, produces a superoxide dismutase (SOD) as a major protein in culture supernatants. This protein was purified by a series of column chromatographic separations. The N-terminal amino acid sequence of the protein was determined to be highly homologous to manganese superoxide dismutase of Escherichia coli or Salmonella reported. The gene (sodA) encoding for SOD of P. alcalifaciens was cloned and sequenced. The sodA-encoded protein has a molecular weight of about 23.5 kDa, and the DNA sequence of P. alcalifaciens sodA gene (627 bp) has about 83% identity to the E. coli SOD gene. We constructed a sodA deletion mutant and its complemented strain of P. alcalifaciens. In J774, a macrophage cell line, the sodA deletion mutant was more susceptible to killing by macrophages than the wildtype strain and its complemented strain. When we injected the mutant strain, its complemented strain and wildtype strain intraperitoneally into DDY strain mice, we found that the sodA deletion mutant proved significantly less virulent while the complemented strain recovered the virulence to the same level of wildtype strain of P. alcalifaciens. These results suggested that manganese superoxide dismutase plays an important role in intracellular survival of P. alcalifaciens.