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1.
Cells ; 10(6)2021 06 10.
Artigo em Inglês | MEDLINE | ID: mdl-34200572

RESUMO

The implications of the microbiome on Coronavirus disease 2019 (COVID-19) prognosis has not been thoroughly studied. In this study we aimed to characterize the lung and blood microbiome and their implication on COVID-19 prognosis through analysis of peripheral blood mononuclear cell (PBMC) samples, lung biopsy samples, and bronchoalveolar lavage fluid (BALF) samples. In all three tissue types, we found panels of microbes differentially abundant between COVID-19 and normal samples correlated to immune dysregulation and upregulation of inflammatory pathways, including key cytokine pathways such as interleukin (IL)-2, 3, 5-10 and 23 signaling pathways and downregulation of anti-inflammatory pathways including IL-4 signaling. In the PBMC samples, six microbes were correlated with worse COVID-19 severity, and one microbe was correlated with improved COVID-19 severity. Collectively, our findings contribute to the understanding of the human microbiome and suggest interplay between our identified microbes and key inflammatory pathways which may be leveraged in the development of immune therapies for treating COVID-19 patients.


Assuntos
COVID-19/diagnóstico , Leucócitos Mononucleares/microbiologia , Pulmão/microbiologia , Microbiota/fisiologia , Líquido da Lavagem Broncoalveolar/microbiologia , Líquido da Lavagem Broncoalveolar/virologia , COVID-19/imunologia , COVID-19/microbiologia , COVID-19/virologia , Estudos de Casos e Controles , Humanos , Leucócitos Mononucleares/virologia , Biópsia Líquida , Pulmão/patologia , Pulmão/virologia , Microbiota/genética , Microbiota/imunologia , Prognóstico , RNA Bacteriano/análise , RNA Fúngico/análise , RNA-Seq , SARS-CoV-2/fisiologia
2.
J Invertebr Pathol ; 169: 107279, 2020 01.
Artigo em Inglês | MEDLINE | ID: mdl-31738888

RESUMO

The microsporidian genera Nosema and Vairimorpha comprise a clade described from insects. Currently the genus Nosema is defined as having a dimorphic life cycle characterized by diplokaryotic stages and diplosporoblastic sporogony with two functionally and morphologically distinct spore types ("early" or "primary" and "environmental"). The Vairimorpha life cycle, in addition to a Nosema-type diplokaryotic sporogony, includes an octosporoblastic sporogony producing eight uninucleate spores (octospores) within a sporophorous vesicle. Molecular phylogeny, however, has clearly demonstrated that the genera Nosema and Vairimorpha, characterized by the absence or presence of uninucleate octospores, respectively, represent two polyphyletic taxa, and that octosporogony is turned on and off frequently within taxa, depending on environmental factors such as host species and rearing temperature. In addition, recent studies have shown that both branches of the Vairimorpha-Nosema clade contain species that are uninucleate throughout their life cycle. The SSU rRNA gene sequence data reveal two distinct clades, those closely related to Vairimorpha necatrix, the type species for the genus Vairimorpha, and those closely related to Nosema bombycis, the type species for the genus Nosema. Here, we redefine the two genera, giving priority to molecular character states over those observed at the developmental, structural or ultrastructural levels and present a list of revised species designations. Using this approach, a series of species are renamed (combination novum) and members of two genera, Rugispora and Oligosporidium, are reassigned to Vairimorpha because of their phylogenetic position. Moreover, the family Nosematidae is redefined and includes the genera Nosema and Vairimorpha comprising a monophyletic lineage of Microsporidia.


Assuntos
Microsporídios/classificação , Nosema/classificação , Filogenia , Características de História de Vida , RNA Fúngico/análise , RNA Ribossômico/análise
3.
Brief Funct Genomics ; 18(6): 367-376, 2019 11 19.
Artigo em Inglês | MEDLINE | ID: mdl-31609411

RESUMO

N6-methyladenosine (m6A) modification, as one of the commonest post-transcription modifications in RNAs, has been reported to be highly related to many biological processes. Over the past decade, several tools for m6A sites prediction of Saccharomyces cerevisiae have been developed and are freely available online. However, the quality of predictions by these tools is difficult to quantify and compare. In this study, an independent dataset M6Atest6540 was compiled to systematically evaluate nine publicly available m6A prediction tools for S. cerevisiae. The experimental results indicate that RAM-ESVM achieved the best performance on M6Atest6540; however, most models performed substantially worse than their performances reported in the original papers. The benchmark dataset Met2614, which was used as the training dataset for the nine methods, were further analyzed by using a position bias index. The results demonstrated the significantly different bias of dataset Met2614 compared with the RNA segments around m6A sites recorded in RMBase. Moreover, newMet2614 was collected by randomly selecting RNA segments from non-redundant data recorded in RMBase, and three different kinds of features were extracted. The performances of the models built on Met2614 and newMet2614 with the features were compared, which shows the better generalization of models built on newMet2614. Our results also indicate the position-specific propensity-based features outperform other features, although they are also easily over-fitted on a biased dataset.


Assuntos
Adenosina/análogos & derivados , RNA/análise , RNA/metabolismo , Saccharomyces cerevisiae/genética , Análise de Sequência de RNA/métodos , Adenosina/metabolismo , Sequência de Bases , Biologia Computacional/métodos , Conjuntos de Dados como Assunto , Aprendizado de Máquina , Processamento Pós-Transcricional do RNA , RNA Fúngico/análise , RNA Fúngico/metabolismo , Transcriptoma
4.
Ear Nose Throat J ; 98(2): 94-97, 2019 Feb.
Artigo em Inglês | MEDLINE | ID: mdl-30813804

RESUMO

BACKGROUND:: Many different etiologies have been proposed to be responsible for the pathogenesis of chronic rhinosinusitis, including dysbiosis of the sinus microbiome. Attempts have recently been made to identify a pathogenic organism via advanced culture mechanisms. The purpose of this study is to use culture-dependent and culture-independent means of microtyping to determine whether any association exists between the quantity and quality of bacteria identified in patients with recalcitrant chronic rhinosinusitis. METHODS:: Medical records were retrospectively reviewed for patients with a history of revision sinus surgery and persistent symptoms who underwent endoscopically directed culture and underwent quantitative polymerase chain reaction analysis of the 16S ribosomal RNA of bacteria and fungi from February 1, 2014, to January 1, 2017. A total of 21 patients met the inclusion criteria. Medical records were reviewed to determine the number of bacterial isolates and relative abundance of bacteria and fungi on culture and polymerase chain reaction. RESULTS:: Using culture-independent techniques of examining purulent secretions in patients with recalcitrant chronic rhinosinusitis, an average of 3.61 isolates were identified per specimen, compared with culture-dependent methods that revealed 2.10 isolates per specimen ( P < .05). The dominant species identified on each culture was rarely the most abundant species identified using polymerase chain reaction techniques. CONCLUSIONS:: Traditional culture methodologies may fail to identify potential pathogens or the dominant pathogen in patients with recalcitrant chronic rhinosinusitis with acute exacerbations.


Assuntos
Bactérias/isolamento & purificação , Disbiose/microbiologia , Fungos/isolamento & purificação , Rinite/microbiologia , Sinusite/microbiologia , Adulto , Idoso , Idoso de 80 Anos ou mais , Doença Crônica , Técnicas de Cultura , Disbiose/complicações , Feminino , Humanos , Masculino , Microbiota , Pessoa de Meia-Idade , Reação em Cadeia da Polimerase , RNA Bacteriano/análise , RNA Fúngico/análise , RNA Ribossômico 16S/análise , Estudos Retrospectivos , Rinite/cirurgia , Sinusite/cirurgia , Adulto Jovem
5.
BMC Infect Dis ; 19(1): 108, 2019 Feb 04.
Artigo em Inglês | MEDLINE | ID: mdl-30717679

RESUMO

BACKGROUND: For definitive diagnosis of cryptococcal meningitis, Cryptococcus neoformans and/or C. gattii must be identified within cerebral spinal fluid from the patients. The traditional methods for detecting Cryptococcus spp. such as India ink staining and culture are not ideal. Although sensitive and specific enough, detection of cryptococcal antigen polysaccharide has a high dose hook effect. Therefore, the aim of this study was to introduce a new rapid and simple detection method of Cryptococcus neoformans and C. gattii in cerebral spinal fluid. METHODS: The lateral flow strips combined with recombinase polymerase amplification (LF-RPA) assay was constructed to detect the specific DNA sequences of C. neoformans and C. gattii. The detection limit was evaluated using serial dilutions of C. neoformans and C. gattii genomic DNA. The specificity was assessed by excessive amount of other pathogens genomic DNA. The optimal detection time and amplification temperature were also analyzed. The diagnostic parameters were first calculated using 114 clinical specimens and then compared with that of other diagnostic method. A brief analysis and comparison of different DNA extraction methods was discussed, too. RESULTS: The LF-RPA assay could detect 0.64 pg of genomic DNA of C. neoformans per reaction within 10 min and was highly specific for Cryptococcus spp.. The system could work well at a wide range of temperature from 25 to 45 °C. The overall sensitivity and specificity were 95.2 and 95.8% respectively. As amplification template for LF-RPA assay, both cell lysates and genomic DNA produce similar experimental results. CONCLUSIONS: The LF-RPA system described here is shown to be a sensitive and specific method for the visible, rapid, and accurate detection of Cryptococcus spp. in cerebral spinal fluid and might be useful for clinical preliminary screening of cryptococcal meningitis.


Assuntos
Criptococose/diagnóstico , Cryptococcus gattii/isolamento & purificação , Cryptococcus neoformans/isolamento & purificação , Meningite Criptocócica/diagnóstico , Reação em Cadeia da Polimerase/métodos , RNA Fúngico/líquido cefalorraquidiano , Antígenos de Fungos/líquido cefalorraquidiano , Antígenos de Fungos/genética , Criptococose/líquido cefalorraquidiano , Cryptococcus gattii/genética , Cryptococcus neoformans/genética , Primers do DNA/genética , Diagnóstico Precoce , Humanos , Limite de Detecção , Meningite Criptocócica/líquido cefalorraquidiano , Técnicas Analíticas Microfluídicas/métodos , RNA Fúngico/análise , Recombinases/genética , Sensibilidade e Especificidade , Temperatura
6.
mSphere ; 3(4)2018 08 08.
Artigo em Inglês | MEDLINE | ID: mdl-30089648

RESUMO

Here, we report an approach to detect diverse bacterial and fungal taxa in complex samples by direct analysis of community RNA in one step using NanoString probe sets. We designed rRNA-targeting probe sets to detect 42 bacterial and fungal genera or species common in cystic fibrosis (CF) sputum and demonstrated the taxon specificity of these probes, as well as a linear response over more than 3 logs of input RNA. Culture-based analyses correlated qualitatively with relative abundance data on bacterial and fungal taxa obtained by NanoString, and the analysis of serial samples demonstrated the use of this method to simultaneously detect bacteria and fungi and to detect microbes at low abundance without an amplification step. Compared at the genus level, the relative abundances of bacterial taxa detected by analysis of RNA correlated with the relative abundances of the same taxa as measured by sequencing of the V4V5 region of the 16S rRNA gene amplified from community DNA from the same sample. We propose that this method may complement other methods designed to understand dynamic microbial communities, may provide information on bacteria and fungi in the same sample with a single assay, and with further development, may provide quick and easily interpreted diagnostic information on diverse bacteria and fungi at the genus or species level.IMPORTANCE Here we demonstrate the use of an RNA-based analysis of specific taxa of interest, including bacteria and fungi, within microbial communities. This multiplex method may be useful as a means to identify samples with specific combinations of taxa and to gain information on how specific populations vary over time and space or in response to perturbation. A rapid means to measure bacterial and fungal populations may aid in the study of host response to changes in microbial communities.


Assuntos
Bactérias/classificação , Biota , Fibrose Cística/microbiologia , Fungos/classificação , RNA Bacteriano/genética , RNA Fúngico/genética , Escarro/microbiologia , Bactérias/genética , Bactérias/isolamento & purificação , Fungos/genética , Fungos/isolamento & purificação , Humanos , Sondas de Oligonucleotídeos/genética , RNA Bacteriano/análise , RNA Fúngico/análise , RNA Ribossômico/análise , RNA Ribossômico/genética , Análise de Sequência de DNA
7.
Int J Med Mushrooms ; 19(2): 163-172, 2017.
Artigo em Inglês | MEDLINE | ID: mdl-28436325

RESUMO

Light is a necessary environmental factor for production of conidia and pigment, formation of stroma, and development of Cordyceps militaris, a well-known edible and medicinal mushroom. In this study, an obvious rhythm loop was observed in certain strains of C. militaris under conditions of alternating 12-hour intervals of dark and light. A possibly related gene, Cmvvd, the homologue of the blue-light photoreceptor of Neurospora crassa, was cloned from the genome of C. militaris. The protein CmVVD is predicted to be 203 amino acids in length and is characterized by the presence of a light, oxygen, or voltage domain. Analysis of the CmVVD sensor domain (light, oxygen, or voltage) suggested that it is a blue-light receptor. Cysteine 108 is essential for the in vivo function of VIVID (VVD) in N. crassa photoadaptation. However, proline is in this position instead in all of the tested CmVVD proteins, suggesting that CmVVD may have a different function or may function in ways different from VVD in N. crassa. Genetic variation analysis of CmVVD in 6 representative strains indicated that 3 informative sites exist. Cmvvd messenger RNA was able to be induced by light, and the expression level increased over 10 times after irradiation and was maintained at high levels in the nascent fruiting body. The light-induced expression of Cmvvd was abolished in Cmwc-1 mutants, suggesting that the expression of Cmvvd is dependent on the photoreceptor CmWC-1 or on a functional CmWC-1/WC-2 complex. This article will help to open the still-unexplored field of circadian rhythms for this fungus.


Assuntos
Cordyceps/genética , Cordyceps/efeitos da radiação , Genes Fúngicos , Luz , Fotorreceptores Microbianos/genética , Clonagem Molecular , Cordyceps/crescimento & desenvolvimento , Cordyceps/metabolismo , Escuridão , Perfilação da Expressão Gênica , Variação Genética , Fotorreceptores Microbianos/biossíntese , Pigmentos Biológicos/metabolismo , Domínios Proteicos , RNA Fúngico/análise , RNA Mensageiro/análise
8.
J Proteome Res ; 14(5): 2207-18, 2015 May 01.
Artigo em Inglês | MEDLINE | ID: mdl-25868943

RESUMO

Infections caused by dermatophytes, Trichophyton rubrum in particular, are among the most common diseases in humans. In this study, we present a proteogenomic analysis of T. rubrum based on whole-genome proteomics and RNA-Seq studies. We confirmed 4291 expressed proteins in T. rubrum and validated their annotated gene structures based on 35 874 supporting peptides. In addition, we identified 323 novel peptides (not present in the current annotated protein database of T. rubrum) that can be used to enhance current T. rubrum annotations. A total of 104 predicted genes supported by novel peptides were identified, and 127 gene models suggested by the novel peptides that conflicted with existing annotations were manually assigned based on transcriptomic evidence. RNA-Seq confirmed the validity of 95% of the total peptides. Our study provides evidence that confirms and improves the genome annotation of T. rubrum and represents the first survey of T. rubrum genome annotations based on experimental evidence. Additionally, our integrated proteomics and multisourced transcriptomics approach provides stronger evidence for annotation refinement than proteomic data alone, which helps to address the dilemma of one-hit wonders (uncertainties supported by only one peptide).


Assuntos
Proteínas Fúngicas/análise , Genoma Fúngico , Peptídeos/análise , Proteoma/análise , RNA Fúngico/análise , Trichophyton/genética , Sequência de Aminoácidos , Bases de Dados de Proteínas , Anotação de Sequência Molecular , Dados de Sequência Molecular , Micélio/química , Micélio/genética , Análise de Sequência de RNA , Esporos Fúngicos/química , Esporos Fúngicos/genética , Trichophyton/química
9.
Rev Iberoam Micol ; 32(1): 59-61, 2015.
Artigo em Espanhol | MEDLINE | ID: mdl-24071639

RESUMO

BACKGROUND: Trichosporonosis is an opportunistic infection caused by the genus Trichosporon. The majority of cases of invasive trichosporonosis occurs in immunocompromised individuals. CASE REPORT: We describe a case of disseminated infection by Trichosporon asahii in a hematology patient. A 52-year-old man diagnosed with acute lymphoblastic leukemia developed a febrile episode during the third cycle of the induction chemotherapy. The blood cultures were positive after 24h incubation, showing elongated structures compatible with fungal elements in the Gram stain. The identification of the fungus as Trichosporon asahii was carried out by the assimilation of compounds of carbon and the amplification and sequencing of the D1/D2 domain and the internal transcribed spacer of the ribosomal DNA. The fungus was also isolated from the pustular lesions that the patient had in the chest. After treatment with amphotericin B, the patient progressed satisfactorily. CONCLUSIONS: Trichosporon asahii is an emergent pathogen in immunosupressed patients and its presence should not be considered as colonization, as there is risk of invasive infection.


Assuntos
Protocolos de Quimioterapia Combinada Antineoplásica/efeitos adversos , Fungemia/microbiologia , Infecções Oportunistas/microbiologia , Leucemia-Linfoma Linfoblástico de Células Precursoras/complicações , Trichosporon/isolamento & purificação , Tricosporonose/etiologia , Anfotericina B/uso terapêutico , Antifúngicos/uso terapêutico , Protocolos de Quimioterapia Combinada Antineoplásica/uso terapêutico , DNA Fúngico/análise , DNA Fúngico/genética , DNA Espaçador Ribossômico/análise , DNA Espaçador Ribossômico/genética , Dermatomicoses/tratamento farmacológico , Dermatomicoses/etiologia , Dermatomicoses/microbiologia , Fungemia/tratamento farmacológico , Fungemia/etiologia , Humanos , Hospedeiro Imunocomprometido , Masculino , Pessoa de Meia-Idade , Técnicas de Tipagem Micológica , Infecções Oportunistas/tratamento farmacológico , Infecções Oportunistas/etiologia , Leucemia-Linfoma Linfoblástico de Células Precursoras/tratamento farmacológico , RNA Fúngico/análise , RNA Fúngico/genética , RNA Ribossômico/análise , RNA Ribossômico/genética , Tricosporonose/tratamento farmacológico
10.
Pathologe ; 34(6): 528-33, 2013 Nov.
Artigo em Alemão | MEDLINE | ID: mdl-24071866

RESUMO

Deep fungal infections are associated with significant mortality despite the availability of new antifungal agents. The identification of causative fungi is important to define successful antifungal therapies as agents differ in the in vitro susceptibility. Characterization of tissue morphology and cultivation from tissue provide important clues to patient management. Molecular techniques such as PCR-based assays are increasingly being used to identify agents of invasive fungal infections. However, potential contamination limits the use when ubiquitous fungi are targeted. Hybridization with fluorescently labeled probes targeting the ribosomal RNA of fungi is emerging as an alternative identification strategy. Using conserved or variable regions of the rRNA as targets, group or species-specific probes can be synthesized to identify fungal pathogens and localize them in the infectious process. These techniques have been successfully applied to deep fungal infections due to different agents in various organ samples.


Assuntos
Hibridização in Situ Fluorescente , Micoses/patologia , Antifúngicos/uso terapêutico , Aspergilose/tratamento farmacológico , Aspergilose/patologia , Biópsia , Candidíase/tratamento farmacológico , Candidíase/patologia , Diagnóstico Diferencial , Humanos , Mucormicose/tratamento farmacológico , Mucormicose/patologia , Micoses/tratamento farmacológico , Micoses/microbiologia , Valor Preditivo dos Testes , RNA Fúngico/análise , RNA Fúngico/genética , RNA Ribossômico/análise , RNA Ribossômico/genética , Doenças Raras/microbiologia , Doenças Raras/patologia
11.
J Agric Food Chem ; 61(25): 6028-35, 2013 Jun 26.
Artigo em Inglês | MEDLINE | ID: mdl-23706159

RESUMO

In this study the catabolites derived from RNA degradation were assessed in Cava sparkling wines as a consequence of lees autolysis. For this purpose, the changes in the content of adenosine, guanosine, inosine, uridine, hypoxanthine, xanthine, and uric acid were determined by UHPLC-MS/MS, in sparkling wines produced on industrial scale, and aged for 4 years. Uridine is the main nucleoside, and its content increases whenever lees cells are present (sur lie aging). Purines seem to have a fermentative origin, with xanthine the most abundant one. When RNA catabolite amounts in sparkling wines aged with or without lees are compared over time, it can be concluded that lees and their cell degradation play an important role in the evolution of Cava; when lees are removed, RNA catabolite amounts remain unchanged.


Assuntos
Bebidas Alcoólicas/análise , RNA Fúngico/análise , Saccharomyces cerevisiae/metabolismo , Bebidas Alcoólicas/microbiologia , Fermentação , Microbiologia de Alimentos , RNA Fúngico/metabolismo , Saccharomyces cerevisiae/química , Espectrometria de Massas em Tandem , Fatores de Tempo , Uridina/análise
12.
Artigo em Inglês | MEDLINE | ID: mdl-22668429

RESUMO

BACKGROUND: The eating disorders anorexia and bulimia nervosa can cause several systemic and oral alterations related to poor nutrition and induced vomiting; however, the oral microflora of these patients is poorly studied. OBJECTIVE: The aim of this study was to evaluate fungal microflora in the oral cavity of these patients by culture-dependent and culture-independent methods. STUDY DESIGN: Oral rinse samples were cultured to assess the prevalence of Candida species, and the isolates were identified by API system. Microorganism counts were compared by the Mann-Whitney test (5%). Ribotyping, a type of molecular analysis, was performed by sequencing the D1/D2 regions of 28S rRNA. RESULTS: Our results demonstrated that the eating disorder group showed higher oral Candida spp. prevalence with culture-dependent methods and higher species diversity with culture-independent methods. CONCLUSIONS: Eating disorders can lead to an increased oral Candida carriage. Culture-independent identification found greater fungal diversity than culture-dependent methods.


Assuntos
Candida/isolamento & purificação , Transtornos da Alimentação e da Ingestão de Alimentos/microbiologia , Boca/microbiologia , Adulto , Biodiversidade , Candida/classificação , Estudos de Casos e Controles , Contagem de Colônia Microbiana , Técnicas de Cultura , Feminino , Humanos , Masculino , Pessoa de Meia-Idade , Técnicas de Tipagem Micológica , RNA Fúngico/análise , RNA Fúngico/genética , Adulto Jovem
14.
Appl Environ Microbiol ; 78(1): 21-7, 2012 Jan.
Artigo em Inglês | MEDLINE | ID: mdl-22020508

RESUMO

Fungal laccases have been widely used in industry. The expression of laccase often is repressible by the primary carbon source glucose in many fungi. The underlying basis is largely unclear. We demonstrate here that a gene, TSP2-1, was required for laccase repression by glucose in the basidiomycete Cryptococcus neoformans. TSP2-1 encodes a Tsp2-type tetraspanin. The disruption of TSP2-1 resulted in constant melanin formation and the expression of the laccase gene LAC1. This derepression phenotype was restorable by 10 mM exogenous cyclic AMP (cAMP). A capsule defect in the mutant tsp2-1Δ also was restored by cAMP. The results indicate an interaction of Tsp2-1 with the cAMP-dependent protein kinase A (PKA) pathway that has been shown to modulate laccase repression and capsule biosynthesis in this fungus. Other roles of TSP2-1, e.g., in maintaining cell membrane integrity and stress resistance, also were defined. This work reveals a Tsp2-1-dependent glucose repression in C. neoformans. The function of Tsp2-type tetraspanin Tsp2-1 is described for the first time.


Assuntos
Cryptococcus neoformans/metabolismo , Proteínas Fúngicas/metabolismo , Glucose/metabolismo , Lacase/metabolismo , Tetraspaninas/metabolismo , Southern Blotting , Cryptococcus neoformans/genética , Proteínas Quinases Dependentes de AMP Cíclico/metabolismo , DNA Fúngico/análise , DNA Fúngico/genética , Proteínas Fúngicas/genética , Melaninas/metabolismo , RNA Fúngico/análise , Reação em Cadeia da Polimerase Via Transcriptase Reversa , Estresse Fisiológico , Tetraspaninas/genética
15.
J Invertebr Pathol ; 108(3): 156-66, 2011 Nov.
Artigo em Inglês | MEDLINE | ID: mdl-21854783

RESUMO

We describe a microsporidian parasite infecting non-native Chinese mitten crabs (Eriochier sinensis) from Europe. Electron microscopy revealed merogonic and sporogonic life stages bound within a plasmalemma. The crab parasite develops polar tube precursors at the sporont stage but does not complete formation of the intact spore extrusion apparatus at the stage of the sporogonial plasmodium like Enterocytozoon bienuesi and other representatives of the Enterocytozoonidae. Its presence within an aquatic crustacean host, and a distinct molecular phylogeny based on partial small subunit ribosomal RNA (SSU rRNA) gene sequences also place it relatively close, though distinct to, existing genera within the Enterocytozoonidae. Consideration of morphological and phylogenetic characteristics of other hepatopancreas-infecting microsporidia from crustaceans suggests that certain ones (e.g. Enterospora canceri) are retained within the clade corresponding to the existing family Enterocytozoonidae, while others, including the parasite described here, may eventually be grouped in a sister taxon potentially of family rank. Based upon morphological and host similarity, it is likely that the parasite described here is the same as Endoreticulatus eriocheir (Wang and Chen, 2007), previously described from Chinese mitten crabs in Asia. However, using a combined taxonomic approach based upon morphological and phylogenetic data, we propose the formation of a new genus (Hepatospora) to replace the previous generic classification of the Asian parasite as Endoreticulatus. The microsporidian from the hepatopancreas of E. sinensis is named Hepatospora eriocheir (Wang and Chen, 2007) gen. et comb. nov. It is assumed that the parasite was introduced during initial invasions of this crab to Europe during the early 20th Century.


Assuntos
Braquiúros/parasitologia , Microsporídios/genética , Microsporidiose/veterinária , Animais , Braquiúros/fisiologia , Europa (Continente) , Hepatopâncreas/parasitologia , Hepatopâncreas/patologia , Interações Hospedeiro-Patógeno , Estágios do Ciclo de Vida/fisiologia , Microsporídios/classificação , Microsporídios/crescimento & desenvolvimento , Microsporidiose/parasitologia , Microsporidiose/patologia , Filogenia , RNA Fúngico/análise , Subunidades Ribossômicas Menores/genética
16.
J Periodontal Res ; 46(3): 303-9, 2011 Jun.
Artigo em Inglês | MEDLINE | ID: mdl-21261623

RESUMO

BACKGROUND AND OBJECTIVE: Chronic infections, such as periodontitis, have been associated with the development and progression of atherosclerosis. The mechanisms through which this occurs have yet to be elucidated. This study was carried out to detect periodontopathic bacteria as well as archaea and fungi in atheromatous plaques and search for factors associated with their occurrence in atheromas. MATERIAL AND METHODS: A cross-sectional study was carried out including 30 patients diagnosed with atherosclerosis in the carotid, coronary or femoral arteries. Plaques were collected during surgery and analysed using PCR to detect Aggregatibacter actinomycetemcomitans, Porphyromonas gingivalis, Treponema denticola and members of the Synergistetes group. Samples were also surveyed with universal primers for bacterial, archaeal and fungal DNA. Patients responded to a questionnaire to determine factors associated with PCR results. RESULTS: All dentate individuals (66.7%) had periodontal disease, 95% of which was severe and 65% extensive. None of the targeted periodontopathic bacteria was found in the atheromas. No sample yielded positive results for fungal and archaeal DNA. Four samples (13%) were positive for the presence of bacterial DNA. Of these, three participants were dentate (two with severely chronic generalized periodontitis and one with severely chronic localized periodontitis). CONCLUSION: This study did not confirm previous findings of periodontal pathogens in atheromas, making it impossible to establish factors associated with their presence in plaques. Presence of bacterial DNA in some samples indicates that periodontal or nonoral bacterial species other than the ones targeted in this study may be involved with some cases of atherosclerosis.


Assuntos
DNA Arqueal/análise , DNA Bacteriano/análise , DNA Fúngico/análise , Periodontite/microbiologia , Placa Aterosclerótica/microbiologia , Idoso , Aggregatibacter actinomycetemcomitans/isolamento & purificação , Bactérias Anaeróbias/isolamento & purificação , Doenças das Artérias Carótidas/microbiologia , Periodontite Crônica/microbiologia , Doença das Coronárias/microbiologia , Estudos Transversais , Feminino , Artéria Femoral/microbiologia , Hemorragia Gengival/microbiologia , Humanos , Masculino , Pessoa de Meia-Idade , Perda da Inserção Periodontal/microbiologia , Porphyromonas gingivalis/isolamento & purificação , RNA Arqueal/análise , RNA Bacteriano/análise , RNA Fúngico/análise , RNA Ribossômico 16S/análise , Treponema denticola/isolamento & purificação
17.
Anal Chem ; 82(21): 9028-33, 2010 Nov 01.
Artigo em Inglês | MEDLINE | ID: mdl-20929247

RESUMO

The utility and performance of label-free, oligonucleotide probes for reagentless detection of dilute target analytes was examined using a voltammetric transduction principle in an array format. Multistep, solid-state fabrication yielded preproduction arrays of 16 individually addressable, 30 µm diameter microelectrodes in a 30 mm × 6.5 mm × 0.5 mm dipstick disposable device. The specificity of 16 nucleotide (nt) 2'-O-methylribonucleic acid and 22 nt DNA backbone probes bound through Mg(2+)-phosphonate bridges to polypyrrole films on the microelectrodes were studied using microbial target RNAs of various lengths. Probe-specific interactions with Escherichia coli O157 H7 23S rRNA (2907 nt) and Candida albicans 18S rRNA (1788 nt) were detected at 65 and 58 fmol/mL, respectively, in volumes as low as 0.5 mL. Specificity studies showed that, for a given probe, "nontarget" transcripts can contribute to changes in the voltammetric detection signal, though with responses that never exceed 70% of the detection signal acquired for specifically designed complementary targets. These results statistically validate the use of the voltammetric microelectrode array for obtaining a "yes-no" answer on complementary specific binding. The study also identifies challenges and pitfalls for the selection strategies of oligonucleotide probes.


Assuntos
Candida albicans/isolamento & purificação , Escherichia coli O157/isolamento & purificação , Análise de Sequência com Séries de Oligonucleotídeos/instrumentação , Sondas de Oligonucleotídeos/química , RNA Bacteriano/análise , RNA Fúngico/análise , Candida albicans/genética , Candidíase/microbiologia , Desenho de Equipamento , Infecções por Escherichia coli/microbiologia , Escherichia coli O157/genética , Humanos , Microeletrodos
18.
J Microbiol Biotechnol ; 20(9): 1322-30, 2010 Sep.
Artigo em Inglês | MEDLINE | ID: mdl-20890098

RESUMO

Three types of nuruk were made from rice, wheat, and a rice-glasswort (6:4) mixture. Nuruk, makgeolli, and vinegar were manufactured with rice nuruk (RN), wheat nuruk (WN), and rice-glasswort nuruk (RGN). The saccharifying activity and ethanol productivity of nuruk, polyphenol content in makgeolli, and acetic acid and polyphenol content in the vinegar were increased as the result of the addition of glasswort. The variable region of 18S- or 16S-rDNA amplified with genomic DNA extracted directly from nuruk, makgeolli- and vinegar-making cultures was analyzed via temperature gradient gel electrophoresis (TGGE). The sequence of the 18S-rDNA variable region extracted from the TGGE gel for nuruk was more than 95% homologous with Aspergillus sp and that for the makgeolli-making culture was more than 95% homologous with Saccharomyces sp and Saccharomycodes sp. The sequence of the 16S-rDNA variable region extracted from TGGE gel for the vinegar-making culture was more than 95% homologous, primarily with the Acetobacter sp. The eukaryotic and prokaryotic diversity in the nuruk, makgeolli-making, and vinegar-making cultures was not significantly altered by the addition of glasswort. Prokaryotic diversity was higher than eukaryotic diversity in the nuruk, but eukaryotic diversity was higher than prokaryotic diversity in the makgeolli-making culture, on the basis of the TGGE patterns. No 18S-rDNA was amplified from the DNA extracted from the vinegar-making culture. In conclusion, the glasswort may be not simply an activator for the growth of microorganisms during the fermentation of nuruk, makgeolli, or vinegar, but also a nutritional supplement that improves the quality of vinegar.


Assuntos
Ácido Acético/metabolismo , Chenopodiaceae/metabolismo , Microbiologia de Alimentos , Ácido Acético/análise , Acetobacter/genética , Acetobacter/isolamento & purificação , Aspergillus/genética , Aspergillus/isolamento & purificação , Fermentação , Oryza/metabolismo , RNA Bacteriano/análise , RNA Fúngico/análise , RNA Ribossômico 16S/análise , RNA Ribossômico 18S/análise , Saccharomyces/genética , Saccharomyces/isolamento & purificação , Triticum/metabolismo
19.
Fungal Genet Biol ; 46(9): 707-13, 2009 Sep.
Artigo em Inglês | MEDLINE | ID: mdl-19460452

RESUMO

Zinc plays a critical role in a diverse array of biochemical processes. However, excess of zinc is deleterious to cells. Therefore, cells require finely tuned homeostatic mechanisms to balance uptake and storage of zinc. Here we show that iron starvation affects zinc metabolism by downregulating expression of the plasma membrane zinc importer encoding zrfB and upregulating the putative vacuolar zinc transporter-encoding zrcA in Aspergillus fumigatus. Nevertheless, the zinc content of iron-starved mycelia exceeded that of iron replete mycelia, possibly due to unspecific metal uptake induced by iron starvation. In agreement with increased zinc excess and zinc toxicity during iron starvation, deficiency in siderophore-mediated high-affinity iron uptake caused hypersensitivity to zinc. Moreover, an increase of zinc uptake by conditional overexpression of zrfB was more toxic under iron depleted compared to iron replete conditions. This deregulated zinc uptake under iron starvation caused a decrease in heme production and an increase in protoporphyrin IX accumulation. Furthermore, zinc excess impaired production of the extracellular siderophore triacetylfusarinine C but not the intracellular siderophore ferricrocin. Taken together, these data demonstrate a fine tuned coordination of zinc and iron metabolism in A. fumigatus.


Assuntos
Aspergillus fumigatus/metabolismo , Ferro/metabolismo , Zinco/metabolismo , Aspergillus fumigatus/genética , Proteínas de Transporte/biossíntese , Proteínas de Transporte/genética , Compostos Férricos/metabolismo , Ferricromo/análogos & derivados , Ferricromo/metabolismo , Proteínas Fúngicas/biossíntese , Proteínas Fúngicas/genética , Regulação Fúngica da Expressão Gênica , Heme/biossíntese , Homeostase , Ácidos Hidroxâmicos/metabolismo , Micélio/metabolismo , Protoporfirinas/metabolismo , RNA Fúngico/análise , RNA Fúngico/biossíntese , RNA Fúngico/genética
20.
Microbiology (Reading) ; 155(Pt 8): 2795-2808, 2009 Aug.
Artigo em Inglês | MEDLINE | ID: mdl-19406900

RESUMO

Paracoccidioides brasiliensis is a thermally dimorphic fungus, and causes the most prevalent systemic mycosis in Latin America. Infection is initiated by inhalation of conidia or mycelial fragments by the host, followed by further differentiation into the yeast form. Information regarding gene expression by either form has rarely been addressed with respect to multiple time points of growth in culture. Here, we report on the construction of a genomic DNA microarray, covering approximately 25 % of the genome of the organism, and its utilization in identifying genes and gene expression patterns during growth in vitro. Cloned, amplified inserts from randomly sheared genomic DNA (gDNA) and known control genes were printed onto glass slides to generate a microarray of over 12,000 elements. To examine gene expression, mRNA was extracted and amplified from mycelial or yeast cultures grown in semi-defined medium for 5, 8 and 14 days. Principal components analysis and hierarchical clustering indicated that yeast gene expression profiles differed greatly from those of mycelia, especially at earlier time points, and that mycelial gene expression changed less than gene expression in yeasts over time. Genes upregulated in yeasts were found to encode proteins shown to be involved in methionine/cysteine metabolism, respiratory and metabolic processes (of sugars, amino acids, proteins and lipids), transporters (small peptides, sugars, ions and toxins), regulatory proteins and transcription factors. Mycelial genes involved in processes such as cell division, protein catabolism, nucleotide biosynthesis and toxin and sugar transport showed differential expression. Sequenced clones were compared with Histoplasma capsulatum and Coccidioides posadasii genome sequences to assess potentially common pathways across species, such as sulfur and lipid metabolism, amino acid transporters, transcription factors and genes possibly related to virulence. We also analysed gene expression with time in culture and found that while transposable elements and components of respiratory pathways tended to increase in expression with time, genes encoding ribosomal structural proteins and protein catabolism tended to sharply decrease in expression over time, particularly in yeast. These findings expand our knowledge of the different morphological forms of P. brasiliensis during growth in culture.


Assuntos
Regulação Fúngica da Expressão Gênica , Genoma Fúngico , Micélio , Paracoccidioides , Paracoccidioidomicose/microbiologia , Perfilação da Expressão Gênica/métodos , Genes Fúngicos , Humanos , América Latina , Dados de Sequência Molecular , Micélio/genética , Micélio/metabolismo , Análise de Sequência com Séries de Oligonucleotídeos/métodos , Paracoccidioides/genética , Paracoccidioides/metabolismo , Análise de Componente Principal , RNA Fúngico/análise , RNA Fúngico/biossíntese , RNA Fúngico/genética , RNA Mensageiro/análise , RNA Mensageiro/biossíntese , RNA Mensageiro/genética , Fatores de Tempo
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