Assuntos
Herpesvirus Humano 4 , Mononucleose Infecciosa/complicações , Serosite/virologia , Membrana Serosa/virologia , Doença Aguda , Adolescente , Adulto , Idoso , Idoso de 80 Anos ou mais , Criança , Pré-Escolar , Feminino , Humanos , Lactente , Mononucleose Infecciosa/diagnóstico , Masculino , Pessoa de Meia-Idade , Adulto JovemRESUMO
An emaciated subadult free-ranging California sea lion (Csl or Zalophus californianus) died following stranding with lesions similar to 11 other stranded animals characterized by chronic disseminated granulomatous inflammation with necrotizing steatitis and vasculitis, involving visceral adipose tissues in the thoracic and peritoneal cavities. Histologically, affected tissues had extensive accumulations of macrophages with perivascular lymphocytes, plasma cells, and fewer neutrophils. Using viral metagenomics on a mesenteric lymph node six mammalian viruses were identified consisting of novel parvovirus, polyomavirus, rotavirus, anellovirus, and previously described Csl adenovirus 1 and Csl bocavirus 4. The causal or contributory role of these viruses to the gross and histologic lesions of this sea lion remains to be determined.
Assuntos
Linfonodos/patologia , Linfonodos/virologia , Leões-Marinhos/virologia , Serosite/patologia , Serosite/veterinária , Esteatite/patologia , Viroma , Anelloviridae/classificação , Anelloviridae/isolamento & purificação , Animais , Animais Selvagens , California , Feminino , Inflamação , Metagenômica , Parvovirus/classificação , Parvovirus/isolamento & purificação , Polyomavirus/classificação , Polyomavirus/isolamento & purificação , Serosite/virologia , Esteatite/virologiaAssuntos
Exantema/virologia , Hepatite/patologia , Herpes Simples/patologia , Herpesvirus Humano 2/isolamento & purificação , Serosite/virologia , Aciclovir/uso terapêutico , Adulto , Antivirais/uso terapêutico , Dor nas Costas/patologia , Dor nas Costas/virologia , Exantema/patologia , Face/patologia , Feminino , Hepatite/tratamento farmacológico , Hepatite/virologia , Herpes Simples/tratamento farmacológico , Herpes Simples/virologia , Humanos , Região Lombossacral/patologia , Imageamento por Ressonância Magnética , Pescoço/patologia , Serosite/patologiaRESUMO
Previously, we showed that intraperitoneal infection with murine coronavirus strain JHM (JHMV) established a persistent infection with subacute granulomatous serositis in interferon-gamma-deficient C57BL/6 (B6-GKO) mice. Herein, we characterize a variant virus from B6-GKO mice persistently infected with JHMV. Viruses were isolated from ascites at 25 d post-infection and cloned by limiting dilution on DBT cells; one variant was named 25V16G. To compare pathogenicity in vivo, we inoculated 25V16G and JHMV intraperitoneally into 8- to 12-week-old B6-GKO mice. Whereas nearly all of the B6-GKO mice infected with JHMV survived over 14 d, all of those infected with 25V16G died by 9 d post-infection. Histopathological examination revealed that 25V16G induced acute fulminant hepatitis in B6-GKO mice, whereas JHMV caused severe but focal hepatitis. The virus titer of 25V16G in the liver was 50- and 250-fold higher than that of JHMV at 5 and 7 d post-infection, respectively. However, there was no significant difference in viral growth between 25V16G and JHMV in cell lines cultured in vitro. Nucleotide sequencing of the S gene of 25V16G and JHMV revealed a deletion of 29 amino acids encompassing S(511-539), which covers a major cytotoxic T lymphocyte (CTL) epitope in C57BL/6 mice, and two point mutations resulting in amino acid changes in the S protein of 25V16G. One explanation for the greater pathogenicity of 25V16G is that 25V16G escapes CTL-mediated protection in B6-GKO mice. This experimental model may be used to assess the role of IFN-gamma in viral persistence in vivo.