RESUMO
Bluetongue, caused by bluetongue virus (BTV), is a widespread arthropod-borne disease of ruminants that entails a recurrent threat to the primary sector of developed and developing countries. In this work, we report modified vaccinia virus Ankara (MVA) and ChAdOx1-vectored vaccines designed to simultaneously express the immunogenic NS1 protein and/or NS2-Nt, the N-terminal half of protein NS2 (NS21-180). A single dose of MVA or ChAdOx1 expressing NS1-NS2-Nt improved the protection conferred by NS1 alone in IFNAR(-/-) mice. Moreover, mice immunized with ChAdOx1/MVA-NS1, ChAdOx1/MVA-NS2-Nt, or ChAdOx1/MVA-NS1-NS2-Nt developed strong cytotoxic CD8+ T-cell responses against NS1, NS2-Nt, or both proteins and were fully protected against a lethal infection with BTV serotypes 1, 4, and 8. Furthermore, although a single immunization with ChAdOx1-NS1-NS2-Nt partially protected sheep against BTV-4, the administration of a booster dose of MVA-NS1-NS2-Nt promoted a faster viral clearance, reduction of the period and level of viremia and also protected from the pathology produced by BTV infection. IMPORTANCE Current BTV vaccines are effective but they do not allow to distinguish between vaccinated and infected animals (DIVA strategy) and are serotype specific. In this work we have develop a DIVA multiserotype vaccination strategy based on adenoviral (ChAdOx1) and MVA vaccine vectors, the most widely used in current phase I and II clinical trials, and the conserved nonstructural BTV proteins NS1 and NS2. This immunization strategy solves the major drawbacks of the current marketed vaccines.
Assuntos
Vírus Bluetongue/imunologia , Bluetongue/prevenção & controle , Vetores Genéticos/genética , Vaccinia virus/genética , Proteínas não Estruturais Virais/genética , Vacinas Virais/imunologia , Animais , Anticorpos Neutralizantes/imunologia , Anticorpos Antivirais/imunologia , Vírus Bluetongue/classificação , Vetores Genéticos/imunologia , Imunidade Celular , Imunização , Imunogenicidade da Vacina , Sorogrupo , Ovinos , Vacinas de DNA/genética , Vacinas de DNA/imunologia , Vaccinia virus/imunologia , Proteínas não Estruturais Virais/imunologia , Vacinas Virais/administração & dosagem , Vacinas Virais/genéticaRESUMO
Bluetongue (BT) is a severe and economically important disease of ruminants that is widely distributed around the world, caused by the bluetongue virus (BTV). More than 28 different BTV serotypes have been identified in serum neutralisation tests (SNT), which, along with geographic variants (topotypes) within each serotype, reflect differences in BTV outer-capsid protein VP2. VP2 is the primary target for neutralising antibodies, although the basis for cross-reactions and serological variations between and within BTV serotypes is poorly understood. Recombinant BTV VP2 proteins (rVP2) were expressed in Nicotiana benthamiana, based on sequence data for isolates of thirteen BTV serotypes (primarily from Europe), including three 'novel' serotypes (BTV-25, -26 and -27) and alternative topotypes of four serotypes. Cross-reactions within and between these viruses were explored using rabbit anti-rVP2 sera and post BTV-infection sheep reference-antisera, in I-ELISA (with rVP2 target antigens) and SNT (with reference strains of BTV-1 to -24, -26 and -27). Strong reactions were generally detected with homologous rVP2 proteins or virus strains/serotypes. The sheep antisera were largely serotype-specific in SNT, but more cross-reactive by ELISA. Rabbit antisera were more cross-reactive in SNT, and showed widespread, high titre cross-reactions against homologous and heterologous rVP2 proteins in ELISA. Results were analysed and visualised by antigenic cartography, showing closer relationships in some, but not all cases, between VP2 topotypes within the same serotype, and between serotypes belonging to the same 'VP2 nucleotype'.
Assuntos
Vírus Bluetongue/classificação , Vírus Bluetongue/genética , Proteínas do Capsídeo/classificação , Proteínas do Capsídeo/genética , Reações Cruzadas/imunologia , Sorogrupo , Animais , Antígenos Virais/imunologia , Bluetongue/imunologia , Bluetongue/virologia , Vírus Bluetongue/imunologia , Proteínas do Capsídeo/imunologia , Ensaio de Imunoadsorção Enzimática , Feminino , Coelhos/imunologia , Ruminantes/imunologia , Sorotipagem , Ovinos/imunologia , Nicotiana/genéticaRESUMO
Bluetongue virus (BTV) is the causative agent of bluetongue disease (BT), which affects domestic and wild ruminants. At the present, 27 different serotypes have been documented. Vaccination has been demonstrated as one of the most effective methods to avoid viral dissemination. To overcome the drawbacks associated with the use of inactivated and attenuated vaccines we engineered a new recombinant BTV vaccine candidate based on proteins VP2, VP7, and NS1 of BTV-4 that were incorporated into avian reovirus muNS-Mi microspheres (MS-VP2/VP7/NS1) and recombinant modified vaccinia virus Ankara (rMVA). The combination of these two antigen delivery systems in a heterologous prime-boost vaccination strategy generated significant levels of neutralizing antibodies in IFNAR(-/-) mice. Furthermore, this immunization strategy increased the ratio of IgG2a/IgG1 in sera, indicating an induction of a Th1 response, and elicited a CD8 T cell response. Immunized mice were protected against lethal challenges with the homologous serotype 4 and the heterologous serotype 1 of BTV. All these results support the strategy based on microspheres in combination with rMVAs as a promising multiserotype vaccine candidate against BTV.
Assuntos
Vírus Bluetongue/imunologia , Bluetongue/prevenção & controle , Imunidade Celular/imunologia , Imunidade Humoral/imunologia , Microesferas , Receptor de Interferon alfa e beta/imunologia , Vacinação , Vaccinia virus/imunologia , Animais , Anticorpos Neutralizantes , Vírus Bluetongue/classificação , Linfócitos T CD8-Positivos/metabolismo , Proteínas do Capsídeo/genética , Proteínas do Capsídeo/imunologia , Linhagem Celular , Chlorocebus aethiops , Imunização , Imunização Secundária/métodos , Imunoglobulina G/sangue , Proteína 1 de Membrana Associada ao Lisossomo/metabolismo , Masculino , Camundongos , Camundongos da Linhagem 129 , Orthoreovirus Aviário/genética , Orthoreovirus Aviário/imunologia , Sorogrupo , Vacinas Atenuadas/imunologia , Vacinas de Produtos Inativados/imunologia , Vacinas Sintéticas/imunologia , Células Vero , Proteínas do Core Viral/genética , Proteínas do Core Viral/imunologia , Proteínas não Estruturais Virais/genética , Proteínas não Estruturais Virais/imunologia , Vacinas Virais/imunologiaRESUMO
ABSTRACT This communication reports full genome sequencing of the bluetongue virus-1 (BTV-1) isolate MKD20/08/Ind from goat in northern India. The total BTV-1 genome size was found to be 19,190 bp. A comparison study between the Indian isolate and other global isolates revealed that it belongs to the 'Eastern' BTV topotype. The full genome sequence of BTV-1 will provide vital information on its geographical origin and it will also be proved useful for comparing the Indian isolate with global isolates from other host species.
Assuntos
Animais , Cabras/virologia , Genoma Viral , Análise de Sequência de DNA , Vírus Bluetongue/genética , Filogenia , Vírus Bluetongue/isolamento & purificação , Vírus Bluetongue/classificação , Genômica , Sequenciamento de Nucleotídeos em Larga Escala , Sorogrupo , ÍndiaRESUMO
In March 2013, EDTA-blood and serum samples were collected from 119 cattle and 159 dromedaries at the slaughterhouse of Nouakchott, the capital city of the Islamic Republic of Mauritania. Serum samples were screened for the presence of Bluetongue (BT) antibodies by competitive ELISA (cELISA). Positive samples were then tested by serum-neutralization (SN) to determine BTV serotype. RNA from blood samples was first tested by a genus-specific quantitative RT-PCR assay which is able to detect all 27 existing BTV serotypes (RT-qPCR1-27). Positive samples were further screened by a RT-qPCR assay which, instead, is able to detect the classical 24 BTV serotypes only (RT-qPCR1-24). Of the 278 serum samples tested, 177 (mean=63.7%; 95% CI: 57.9%-69.1%) resulted positive by cELISA. Of these, 69 were from cattle (mean=58.0%; 95% CI: 49.0%-66.5%) and 108 from dromedaries (mean=67.9%; 95% CI: 60.3%-74.7%). BTV-26 neutralizing antibodies were by far the most frequently found as they were detected in 146 animals with titres ranging from 1:10 to 1:80. Out of 278 blood samples, 25 (mean=9.0%; 95% CI: 6.2%-12.9%) were found positive for BTV by RT-qPCR1-27, 20 (mean=16.8%; 95% CI: 11.2%-24.6%) were from cattle and 5 (mean=3.1%; 95% CI: 1.4%-7.1%) from dromedaries. When tested by RT-qPCR1-24 the 25 BTV positive samples were negative. Unfortunately, no genetic information by molecular typing or by next generation sequencing has been obtained as for the very low levels of RNA in the blood samples.
Assuntos
Vírus Bluetongue/classificação , Bluetongue/epidemiologia , Camelus/virologia , Doenças dos Bovinos/virologia , Animais , Bluetongue/virologia , Vírus Bluetongue/genética , Bovinos , Programas de Rastreamento/métodos , Mauritânia/epidemiologia , Vigilância da População , Sorogrupo , Sorotipagem , Ovinos/virologiaRESUMO
Bluetongue virus is the "type" species of the genus Orbivirus, family Reoviridae. Twenty four distinct bluetongue virus (BTV) serotypes have been recognized for decades, any of which is thought to be capable of causing "bluetongue" (BT), an insect-borne disease of ruminants. However, two further BTV serotypes, BTV-25 (Toggenburg orbivirus, from Switzerland) and BTV-26 (from Kuwait) have recently been identified in goats and sheep, respectively. The BTV genome is composed of ten segments of linear dsRNA, encoding 7 virus-structural proteins (VP1 to VP7) and four distinct non-structural (NS) proteins (NS1 to NS4). We report the entire BTV-26 genome sequence (isolate KUW2010/02) and comparisons to other orbiviruses. Highest identity levels were consistently detected with other BTV strains, identifying KUW2010/02 as BTV. The outer-core protein and major BTV serogroup-specific antigen "VP7" showed 98% aa sequence identity with BTV-25, indicating a common ancestry. However, higher level of variation in the nucleotide sequence of Seg-7 (81.2% identity) suggests strong conservation pressures on the protein of these two strains, and that they diverged a long time ago. Comparisons of Seg-2, encoding major outer-capsid component and cell-attachment protein "VP2" identified KUW2010/02 as 26th BTV, within a 12th Seg-2 nucleotype [nucleotype L]. Comparisons of Seg-6, encoding the smaller outer capsid protein VP5, also showed levels of nt/aa variation consistent with identification of KUW2010/02 as BTV-26 (within a 9th Seg-6 nucleotype - nucleotype I). Sequence data for Seg-2 of KUW2010/02 were used to design four sets of oligonucleotide primers for use in BTV-26, type-specific RT-PCR assays. Analyses of other more conserved genome segments placed KUW2010/02 and BTV-25/SWI2008/01 closer to each other than to other "eastern" or "western" BTV strains, but as representatives of two novel and distinct geographic groups (topotypes). Our analyses indicate that all of the BTV genome segments have evolved under strong purifying selection.
Assuntos
Vírus Bluetongue/genética , Genoma Viral/genética , Animais , Vírus Bluetongue/classificação , Cabras , Kuweit , Filogenia , Reação em Cadeia da Polimerase Via Transcriptase Reversa , Ovinos , Proteínas não Estruturais Virais/genética , Proteínas Estruturais Virais/genéticaRESUMO
Bluetongue virus, a member of the genus Orbivirus of the family Reoviridae, is the causative agent of bluetongue, which is a non-contagious Culicoides mediated blood-borne disease. The present study characterizes the pathogenicity of a Taiwan prototype BTV2/KM/2003 in Corriedale sheep inoculated subcutaneously into the ear pinna. Histologically, multifocal petechiated hemorrhage, with mild to moderate inflammation and edema, were present in the contralateral ear pinna, tongue, and facial skin, without remarkable lesions in lymphoid organs. By days post-infection (DPI) 7, viral VP7 antigen, detected by immunohistochemistry, presented in the spleen, chiefly located in the outer rim of <3 cell thickness of marginal zone macrophages bordering the marginal zone and red pulp, and T lymphocytes of the red pulp. By DPI 11, viral signals shifted from the marginal zone to macrophages and small lymphocytes within follicles of the spleen. In situ hybridization with VP7 gene probe detected strong signals in the spleen, chiefly spanning the whole width of 5-10 cell thickness of the marginal zone, including the marginal zone macrophages and marginal zone B cells, as well as macrophages of sheathed capillaries in the red pulp. This study demonstrates molecular as well as morphologic evidence of the presence of bluetongue virus in the marginal zone of the spleen, most likely associated with viremia in acute infection, as previously demonstrated by the authors.
Assuntos
Vírus Bluetongue/isolamento & purificação , Bluetongue/virologia , Carneiro Doméstico/virologia , Baço/virologia , Animais , Linfócitos B/virologia , Bluetongue/patologia , Vírus Bluetongue/classificação , Vírus Bluetongue/patogenicidade , Linhagem Celular , Cricetinae , Imuno-Histoquímica , Hibridização In Situ , Macrófagos/virologia , Masculino , Linfócitos T/virologia , Proteínas do Core Viral/isolamento & purificaçãoRESUMO
In the present study, group-specific antigen VP7 of bluetongue virus (BTV) serotype 21 isolated from cattle in Tochigi prefecture in Japan in 1994 was characterized by sequencing and expression. Gene was amplified from cDNA synthesized on viral dsRNA using reverse-transcriptase-PCR. Nucleotide sequence of this isolate showed high similarity with other published BTV VP7 sequences. Full-length and C-terminal truncated forms of VP7 were expressed in insect cells by a baculovirus gene expression system under control of the viral polyhedrin promoter. Expression of full-length recombinant VP7 was confirmed by immunoprecipitation with VP7 specific monoclonal antibody (8A3B.6, ATCC). Recombinant proteins expressed with or without 6x His-tag showed good expression levels in TN5 cells and reacted well with the monoclonal antibody in the indirect ELISA. However C-terminal truncated VP7 with His-tag failed to react with this monoclonal antibody, while poor antigenicity was evident when it was reacted with infected bovine serum. Reduced antigenicity of the latter suggested that C-terminal truncation affects 8A3B.6 epitope construction probably via inhibition of VP7 trimer structure formation.
Assuntos
Vírus Bluetongue/metabolismo , Bluetongue/virologia , Doenças dos Bovinos/virologia , Regulação Viral da Expressão Gênica/fisiologia , Proteínas do Core Viral/metabolismo , Animais , Antígenos Virais/genética , Antígenos Virais/metabolismo , Bluetongue/epidemiologia , Vírus Bluetongue/classificação , Vírus Bluetongue/genética , Vírus Bluetongue/imunologia , Bovinos , Doenças dos Bovinos/epidemiologia , Linhagem Celular , Cricetinae , Japão/epidemiologia , Sorotipagem , Proteínas do Core Viral/genéticaRESUMO
The role of apoptosis in pathogenesis of bluetongue (BT) has been suggested from various in vitro studies. However, to date, no clear data are available regarding BTV-induced apoptosis and its consequences in natural host, sheep. In the present study, bluetongue virus (BTV)-induced apoptosis was studied in sheep blood and splenic mononuclear cells by analyzing annexin(+)-propidium iodide(-) early apoptotic cells, DNA ladder pattern, and caspase-3 gene expression. The onset of apoptosis and lymphocyte depletion in viraemic phase and IFN-alpha response indicated the involvement of BTV and IFN-alpha in the pathogenesis of BT. The development of Pasteurella pneumonia in 4 of 7 infected sheep during the experiment pointed to possible BTV-induced immuno-suppression and predisposition to secondary microbial infections. These results have significant implications not only in understanding immuno-pathological consequences but also in studying interactions of BTV with host cells.
Assuntos
Vírus Bluetongue/classificação , Bluetongue/virologia , Terapia de Imunossupressão/veterinária , Animais , Apoptose , Caspase 3/genética , Caspase 3/metabolismo , Regulação da Expressão Gênica , Leucócitos Mononucleares/fisiologia , Leucócitos Mononucleares/virologia , Pulmão/citologia , Tecido Linfoide/citologia , Proteínas Proto-Oncogênicas c-bcl-2/genética , Proteínas Proto-Oncogênicas c-bcl-2/metabolismo , Sorotipagem , Ovinos , Baço/citologia , Fatores de Tempo , ViremiaRESUMO
Bluetongue is an arthropod-borne viral disease affecting domestic and wild ruminants. Taiwan, with the Tropic of Cancer crossing through it, was considered free of bluetongue virus (BTV) before 2001. The goals of this study are to identify the serotype and phylogeny of Taiwan BTV isolates and to understand the serological status and chronology of BTV infection. Analysis of the S10 gene segment revealed that Taiwan BTV isolates are closely related to Chinese strains. Seropositive results were found in 32.7% of the cattle and 8.2% of the goats by head, and 90.7% of the cattle herds and 28.9% of the goat flocks. Anti-BTV antibodies have existed in goat sera since 1989 and in bovine sera since 1993, and over the years, the seropositive rates in rapidly urbanized districts have decreased, most likely due to the loss of vector habitats. Seropositive rates for sheep were variable, due to a small sample size and a small sheep population. Thus far, all natural BTV infections have been subclinical, consistent with experimental sheep inoculation, revealing that the Taiwan isolate is of low virulence.
Assuntos
Animais Domésticos/virologia , Vírus Bluetongue/genética , Bluetongue/epidemiologia , Bluetongue/virologia , Animais , Anticorpos Antivirais/sangue , Vírus Bluetongue/classificação , Vírus Bluetongue/isolamento & purificação , Temperatura Corporal , Bovinos , Doenças dos Bovinos/epidemiologia , Doenças das Cabras/epidemiologia , Cabras , Masculino , Dados de Sequência Molecular , Filogenia , Estudos Retrospectivos , Estudos Soroepidemiológicos , Ovinos , Taiwan , Proteínas não Estruturais Virais/genéticaRESUMO
Since 1998, six distinct serotypes of Bluetongue virus (BTV) have invaded Southern and Central Europe, persisting in some regions for up to 6 years and resulting in the deaths of >1.8 million sheep. Rapid and reliable methods of virus detection and identification play an essential part in our fight against bluetongue disease (BT). We have therefore developed and evaluated a duplex, one-step RT-PCR assay that detects genome segment 7 (encoding the major serogroup (virus-species) specific antigen and outer-core-protein VP7) from any of the 24 BTV serotypes. Although Seg-7 is highly conserved, there are sequence differences in the near terminal regions that identify two distinct phylogenetic groups. Two sets of primers (targeting Seg-7 terminal regions of viruses from these two groups) were included in a duplex RT-PCR assay system. Assay sensitivity was evaluated using tissue culture derived virus, infected vector insects and clinical samples (blood and other tissues). The assay reliably amplified Seg-7 from any of the BTV strains tested, including isolates of the 24 BTV serotypes and isolates from different geographic origins. No cross-reactions were detected with members of closely related Orbivirus species (African horsesickness virus (AHSV), Epizootic haemorrhagic disease virus (EHDV), Equine encephalosis virus (EEV) and Palyam virus (PALV)).
Assuntos
Vírus Bluetongue/isolamento & purificação , Bluetongue/diagnóstico , Genes Virais , Reação em Cadeia da Polimerase Via Transcriptase Reversa/métodos , Proteínas do Core Viral/genética , Animais , Bluetongue/virologia , Vírus Bluetongue/classificação , Vírus Bluetongue/genética , Ceratopogonidae/virologia , Primers do DNA , Insetos Vetores/virologia , Sensibilidade e Especificidade , Sorotipagem , Ovinos , Viremia/diagnósticoRESUMO
Bluetongue virus (BTV), a member of genus Orbivirus, a family Reoviridae, is a non-enveloped with double shelled structure and ten segmented double stranded (ds) RNA genome. The RNA segment S7 encodes an inner capsid serogroup specific viral protein VP7. To amplify coding region of VP7 gene of BTV, new primers, forward primer (18-38 bp) and reverse primer (1156-1136 bp), were designed using VP7 gene sequences available in GenBank. This primer pair successfully amplified cell culture adapted Indian isolates of BTV belonging to two different serotypes 1 and 18. The coding sequences of two Indian isolates of BTV (BTV-1H and BTV-18B) were cloned into pPCR Script-Amp SK (+) plasmid vector and transformed into XL10-Gold Kan ultracompetent E. coli cells. The positive clones selected by blue-white screening and colony touch PCR were sequenced. The sequence analysis revealed that there was 93-97% nucleotide sequence identity in VP7 gene of three different Indian serotypes of BTV. The VP7 gene sequences of Indian isolates have comparatively less sequence homology (< 80%) with American (US), and French isolates compared to South African (SA), Australian (AUS) and Chinese (PRC) isolates. In silico restriction enzyme profile analysis of VP7 gene sequences revealed that Indian isolates of BTV-1 can be differentiated from other BTV-1 isolates reported from SA, AUS and PRC using TaqI. Similarly the Indian isolates of BTV belonging to three different serotypes can be differentiated using EcoRI, Hae III and TaqI restriction enzymes.
Assuntos
Vírus Bluetongue/genética , Genes Virais , Proteínas do Core Viral/genética , Sequência de Aminoácidos , Animais , Sequência de Bases , Bluetongue/genética , Bluetongue/metabolismo , Vírus Bluetongue/classificação , Dados de Sequência Molecular , Filogenia , Mapeamento por Restrição , Alinhamento de Sequência , Análise de Sequência de DNA , Homologia de Sequência , OvinosRESUMO
Two bluetongue virus (BTV) serotype 10-specific single-chain Fv chicken antibody fragments (scFvs) were evaluated in a competitive ELISA. The binding of one (F3) to purified BTV was only inhibited by antibodies against the homologous serotype. The binding of the other (F10) was blocked by antisera to each of the 24 BTV serotypes. F10 recognised VP7, a major structural protein of the BTV core, but not if the protein was directly adsorbed to a plastic surface. It did, however, bind to recombinant VP7 that had been captured from suspension by rabbit IgG. This made it possible to develop an scFv based inhibition ELISA for BTV antibodies using recombinant VP7 without prior purification. The resulting immunoassay detected antibodies to 24 BTV serotypes, but not those directed against three serotypes of the related epizootic haemorrhagic disease virus. A phage library displaying fusion peptides expressed by fragments of the BTV genome segment 7 cDNA was constructed and screened using F10. Comparing selected peptides with the amino acid sequence of VP7 showed that recognition by the scFv required at least 131 residues representing the protein's upper domain. By providing well-characterised immunological reagents, recombinant antibody technology can contribute to the development of improved immunoassays for BTV diagnosis.
Assuntos
Anticorpos Antivirais/análise , Antígenos Virais/análise , Vírus Bluetongue/classificação , Ensaio de Imunoadsorção Enzimática/métodos , Animais , Antígenos Virais/química , Antígenos Virais/genética , Antígenos Virais/imunologia , Vírus Bluetongue/genética , Galinhas , Sorotipagem , Proteínas do Core Viral/imunologiaRESUMO
Bluetongue (BT) is a viral disease of domestic and wild ruminants. It is particularly damaging in sheep, where up to half of infected animals may die, showing inflammation and hemorrhages of the mucous membranes of the mouth, nose, and intestines. In cattle and goats, BT rarely causes disease, however it can affect the animal's reproductive ability, so that losses are not easily estimated. Bluetongue virus spreads from animal to animal by biting insects of the genus Culicoides; and this is the reason why the disease is more prevalent in geographic areas where climate conditions are favourable for their development. The disease was first recognized in South Africa in the late 1700's, but it was not until the early 1900's that it was described in detail, and at present, epizootiology and pathogenesis studies are still being carried on.
Assuntos
Animais , Masculino , Feminino , Bluetongue , Vírus Bluetongue , Aborto Animal , Antígenos Virais/imunologia , Argentina , Bluetongue , Ceratopogonidae , Doenças Fetais/veterinária , Doenças Fetais/virologia , Infertilidade Masculina , Insetos Vetores , Proteínas Virais/imunologia , RNA Viral , Ruminantes , Vacinas Virais , Vírus Bluetongue/classificação , Vírus Bluetongue/isolamento & purificação , Vírus Bluetongue/fisiologiaRESUMO
Bluetongue virus (BTV) infection of ruminants is endemic throughout tropical and subtropical regions of the world. The S7 gene segments of prototype Chinese strains of BTV serotypes 1, 2, 3, 4, 12, 15, and 16 were sequenced and compared to the same genes of prototype strains of BTV from the US, Australia, and South Africa. The S7 genes and predicted VP7 proteins of the Chinese viruses were relatively conserved, with the notable exception of serotype 15. Furthermore, phylogenetic analysis of the S7 genes did not predict geographic origin of the various strains of BTV.
Assuntos
Vírus Bluetongue/classificação , Bluetongue/virologia , Filogenia , Proteínas do Core Viral/genética , Animais , Austrália , Vírus Bluetongue/genética , Vírus Bluetongue/metabolismo , Bovinos , China , Dados de Sequência Molecular , Ovinos , África do Sul , Estados UnidosRESUMO
Bluetongue virus (BTV) is an arthropod-borne orbivirus that infects sheep, wild ruminants and occasionally cattle. Detection and specific identification of BTV is a multistep process. The first step involves the isolation of the virus from the animal's blood or other tissues, followed by inoculation of embryonating chicken eggs (ECE). After the virus has been amplified in ECE, it is passaged into BHK-21 cell culture for subsequent replication and identification. The virus is then amplified further and identified in microtiter plates by the immunoperoxidase assay using a group specific monoclonal antibody. Finally, the viral isolate is typed by a virus neutralization test.
Assuntos
Vírus Bluetongue/isolamento & purificação , Bluetongue/virologia , Animais , Anticorpos Monoclonais , Anticorpos Antivirais , Bluetongue/sangue , Vírus Bluetongue/classificação , Bovinos , Linhagem Celular , Embrião de Galinha , Efeito Citopatogênico Viral , Técnicas Imunoenzimáticas , Testes de Neutralização , Vísceras/virologiaRESUMO
Core particles of bluetongue virus serotype 1 (South Africa) have been crystallized. The crystals, which grow up to 0.8 mm in diameter, belong to a primitive orthorhombic space group and have point group symmetry 222. The unit cell dimensions are 754 x 796 x 823 A3 and the crystallographic asymmetric unit contains one-half of a core particle. The best crystals diffract strongly to 4.8 A Bragg spacings, which is the maximum resolution to which we can measure data with the detectors available, suggesting that useful diffraction extends well beyond this. Core particles of serotype 10 have also been crystallized but the crystals have yet to be analyzed by X-ray diffraction.
Assuntos
Vírus Bluetongue/química , Proteínas do Core Viral/química , Proteínas do Core Viral/ultraestrutura , Animais , Vírus Bluetongue/classificação , Vírus Bluetongue/ultraestrutura , Bovinos , Cristalização , Cristalografia por Raios X/métodos , Cabras , Ruminantes , Ovinos , África do Sul , Difração de Raios X/métodosRESUMO
The nucleotide sequence of the RNA segment 3 of bluetongue virus (BTV) serotype 2 (Ona-A) from North America was determined to be 2772 nucleotides containing a single large open reading frame of 2703 nucleotides (901 amino acid). The predicted VP3 protein exhibited general physiochemical properties (including hydropathy profiles) which were very similar to those previously deduced for other BTV VP3 proteins. Partial genome segment 3 sequences, obtained by polymerase chain reaction (PCR) sequencing, of BTV isolates from the Caribbean were compared to those from North America, South Africa, India, Indonesia, Malaysia and Australia, as well as other orbiviruses, to determine the phylogenetic relationships amongst them. Three major BTV topotypes (Gould, A.R. (1987) Virus Res. 7, 169-183) were observed which had nucleotide sequences that differed by approximately 20%. At the molecular level, geographic separation had resulted in significant divergence in the BTV genome segment 3 sequences, consistent with the evolution of distinct viral populations. The close phylogenetic relationship between the BTV serotype 2 (Ona-A strain) from Florida and the BTV serotypes 1, 6 and 12 from Jamaica and Honduras, indicated that the presence of BTV serotype 2 in North America was probably due to an exotic incursion from the Caribbean region as previously proposed by Sellers and Maaroof ((1989) Can. J. Vet. Res. 53, 100-102) based on trajectory analysis. Conversely, nucleotide sequence analysis of Caribbean BTV serotype 17 isolates suggested they arose from incursions which originated in the USA, possibly from a BTV population distinct from those circulating in Wyoming.
Assuntos
Vírus Bluetongue/classificação , Orbivirus/classificação , RNA Viral/genética , Proteínas do Core Viral/genética , Sequência de Aminoácidos , Animais , Sequência de Bases , Vírus Bluetongue/genética , Linhagem Celular , Cricetinae , DNA Viral , Dados de Sequência Molecular , Orbivirus/genética , Filogenia , SorotipagemRESUMO
A cytopathic agent was isolated in a baby hamster kidney (BHK)-21 cell-line from blood samples of cross-bred sheep showing typical bluetongue symptoms at Avikanagar in Rajasthan State, India. The cytopathic agent was identified as a bluetongue virus (BTV) by immunofluorescence, the immunoperoxidase test and electron microscopy of BHK-21 cells infected with the new isolate. The new isolate was typed as BTV serotype 1.
Assuntos
Vírus Bluetongue/isolamento & purificação , Bluetongue/virologia , Animais , Vírus Bluetongue/classificação , Vírus Bluetongue/ultraestrutura , Linhagem Celular , Cricetinae , Efeito Citopatogênico Viral , Imunofluorescência , Técnicas Imunoenzimáticas , Índia , Microscopia Eletrônica , Sorotipagem/veterinária , OvinosRESUMO
The genetic characteristics and phylogenetic relationships between the U.S. prototype strain of bluetongue virus serotype 10 (BTV 10), the modified live virus vaccine currently used in California, and three field isolates of BTV 10 obtained in 1980 and three in 1990 in California were determined by comparison of their L2 gene sequences. The L2 genes of the 1980 field isolates were very closely related to the L2 genes of the prototype strain and the vaccine strain, differing by only 0.1 to 0.5%. The 1990 field isolates diverged from all the other viruses by an average of 4.8%. They showed a high degree of genetic similarity that ranged from 98.2 to 99.7% and formed a separate group. All BTV 10 viruses derived from a common ancestor (bootstrap value 100%) from which two different lineages have diverged giving rise to two monophyletic groups, one including all the 1990 viruses and the other the prototype, the vaccine, and all 1980 field strains. The bootstrap analyses placed a 100% confidence value at each of these two nodes. These results indicate that two different lineages of BTV 10 circulated in California between 1953 and 1990. The effect of the vaccine on the evolutionary pathways of the BTV 10 population present in California in 1980 was not clearly established, but it did not influence the evolution of the BTV 10 field isolates obtained in 1990.