RESUMO
This study dealt with the toxicity of inactivated bacteria intended for veterinary autogenous vaccines toward a suitable control assay. Two in vitro methods were used. The [3-(4, 5 -dimethylthiazol-2-yl) -2,5 -diphenyltetrazolium bromide] (MTT) test, based on the metabolic reaction of a tetrazolium salt in vital cells, was adopted on the basis of previous positive results. The Interleukin (IL)-1 beta release assay on monocyte-derived pig macrophages was carried out for comparative purposes, to evaluate the possible role of the inflammatory response. MTT and IL-1 beta responses showed a significant correlation (P < 0.05) at defined test dilutions of bacterial antigens, whereas no correlation was demonstrated using MTT responses normalized on bacterial cell concentration. Furthermore, the toxic effects shown in the MTT test were positively correlated to the extracellular protein content. On the whole, the above results could be a useful basis for the development of a toxicity assay on inactivated bacterial vaccines. Also, our data point at bacterial autolysis as a major component underlying toxicity.
Assuntos
Vacinas Bacterianas/toxicidade , Macrófagos/efeitos dos fármacos , Sus scrofa/fisiologia , Testes de Toxicidade , Animais , Técnicas In Vitro , Sais de Tetrazólio/química , Tiazóis/química , Vacinas de Produtos Inativados/toxicidadeRESUMO
As a result of the high-dose gamma-irradiation (50 kGy) of corpuscular pertussis vaccine (CPV), pertussis radiovaccine (PRV) was obtained. Compared with CPV, PRV was shown to possess reduced toxicity and higher protective potency. Its adjuvant properties remained at the initial level, while its leukocytosis-promoting and histamine-sensitizing activities drastically decreased. Moreover, PRV produced less pronounced stimulating effect on the proliferation of hematopoietic stem cells, but, in contrast to CPV, was shown to capable of protecting mice from lethal radiation.
Assuntos
Vacina contra Coqueluche/imunologia , Vacina contra Coqueluche/efeitos da radiação , Animais , Células Produtoras de Anticorpos/imunologia , Raios gama , Células-Tronco Hematopoéticas/imunologia , Histamina/análogos & derivados , Histamina/imunologia , Imunização , Leucocitose/induzido quimicamente , Camundongos , Camundongos Endogâmicos CBA , Vacina contra Coqueluche/toxicidade , Lesões Experimentais por Radiação/imunologia , Lesões Experimentais por Radiação/mortalidade , Tolerância a Radiação/imunologia , Vacinas de Produtos Inativados/imunologia , Vacinas de Produtos Inativados/efeitos da radiação , Vacinas de Produtos Inativados/toxicidadeRESUMO
Strains X-73 (serotype 1) and P-1059 (serotype 3) of Pasteurella multocida, avian origin, expressed additional membrane proteins (MPs) when grown in brain-heart infusion (BHI) broth containing the iron chelator dipyridyl and when grown in BHI broth treated with the iron chelator Chelex 100. These additional MPs were not detected when both strains were grown in BHI broth. Chickens and turkeys were vaccinated twice with inactivated oil-emulsion vaccines containing bacterial cells expressing these MPs or with vaccines containing bacterial cells grown in BHI broth. Two weeks after the final vaccination, all birds were challenged to determine whether bacterins made from P. multocida that had been propagated in conditions of iron deprivation would induce heterologous serotype immunity. The bacterins produced in medium low in iron did not consistently induce significant protection against heterologous challenge.
Assuntos
Vacinas Bacterianas/administração & dosagem , Infecções por Pasteurella/veterinária , Pasteurella multocida , Doenças das Aves Domésticas , Vacinas de Produtos Inativados/administração & dosagem , Animais , Proteínas de Bactérias/análise , Vacinas Bacterianas/toxicidade , Galinhas , Meios de Cultura , Eletroforese em Gel de Poliacrilamida , Ferro/metabolismo , Infecções por Pasteurella/prevenção & controle , Pasteurella multocida/classificação , Pasteurella multocida/crescimento & desenvolvimento , Perus , Vacinas de Produtos Inativados/toxicidadeRESUMO
Two vaccine preparations obtained from Bordetella pertussis, whole-cell vaccine constituting one of the components of adsorbed DPT vaccine and acellular vaccine, were tested for mutagenicity. The doses of the preparations covered the range 1-100 ED50. Ames' test and the metaphase analysis of marrow cells of C57BL/6J mice were used. The acellular preparation was also tested on thymectomized mice, taking into consideration chromosomal aberrations in marrow metaphases. Whole-cell and acellular pertussis vaccines did not induce mutations in Salmonella typhimurium and chromosomal aberrations in mouse marrow cells.