Hydrogen bond recombination regulated by strongly electronegative functional groups in demulsifiers for efficient separation of oil-water emulsions.

Tight oil extraction and offshore oil spills generate large amounts of oil-water emulsions, causing serious soil and marine pollution. In such oil-water emulsions, the resin molecules are bound by π-π stacking and bind to interfacial water molecules via hydrogen bonds, which impede the aggregation between water droplets and thereby the separation of the emulsion. In this study, strongly electronegative oxygen atoms (in ethylene oxide, propylene oxide, esters, and hydroxyl groups) were introduced through poly(propylene glycol)-block-polyether and esterification with acrylic acid to attract negative charges in order to form electron-rich regions and enhance interfacial hydrogen bond recombination. The potential distribution in the demulsifier molecules and their space occupancy were regulated by the polymerization reaction to destroy the π-π stacking interaction between resin molecules. The results show that the binding energies (binding free energy and hydrogen bonding energy) of oxygen-containing demulsifier molecules with water molecules were higher than those of resin molecules with water molecules, resulting in the fission of the hydrogen bonds between resin and water molecules. The introduction of demulsifier molecules that occupied large interfacial space reduced the binding energy between resin molecules from -2176.06 to -110.00 kJ·mol-1. Noteworthy, the binding energy between demulsifier molecules and resin molecules was -1076.36 kJ·mol-1 lower than that between resin molecules (-110.00 kJ·mol-1), indicating the adsorption of the surrounding interfacial resin molecules by the demulsifier molecules and destruction of the π-π stacking between them, thus favoring the collapse of the interfacial structure of the oil-water emulsion and achieving its separation. This study provides important theoretical support for the treatment of oil-contaminated soil and offshore oil spill pollution.

Overlooked encounter process that affects physical behaviors of stabilized nanoscale zero-valent iron during in situ groundwater remediation.

Dynamic encountering between groundwater matrices and nanoscale zero-valent iron (NZVI) injected for in situ subsurface remediation affects NZVI's mobility and has not been well recognized. Polyacrylic acid (PAA)-stabilized NZVI (NZVI-PAA) and Mg(OH)2-coated NZVI (NZVI@Mg(OH)2) were investigated as representative NZVIs stabilized by enhanced electrostatic repulsion and reduced magnetic attraction, respectively. Encounters with divalent cations and humic acid (HA) induced the drastic aggregation and sedimentation (presedimentation) of NZVI-PAA owing to Lewis acid-base interactions and heteroaggregation. In addition, encountered groundwater electrolytes could not effectively provide electrostatic repulsion for NZVI-PAA, resulting in breakthrough ripening dynamics. The presedimentation and ripening behaviors of NZVI-PAA were eliminated and unheeded after mixing the NZVI slurry with groundwater by sonication. In comparison, the encountering process barely impacted NZVI@Mg(OH)2, for which settling was hindered. Although the particle-collector attraction promoted NZVI@Mg(OH)2 adsorption on pristine and hybrid-coated sands, the Langmuirian blocking dynamics of the NZVI@Mg(OH)2 breakthrough demonstrated its high mobility after adsorption sites of sand surface were exhausted. Extended Derjaguin-Landau-Verwey-Overbeek analysis and transport modeling provided insights into overlooked effects of encountering on physical behaviors of different stabilized NZVIs, which should be considered during practical applications under diverse subsurface conditions.

Sugarcane-based superhydrophilic and underwater superoleophobic membrane for efficient oil-in-water emulsions separation.

The development of ecological, low cost, easy preparation, especially high performance materials for emulsions separation is of great importance due to the rise in pollution of oil-water emulsions from industrial production and domestic waste. Straws as agricultural wastes, including plenty of hydrophilic groups and multi-level pore structures, can be prepared as biomass membranes for oil-water emulsion separation. Herein, a novel super-hydrophilic sugarcane-based (SHS) membrane was prepared using a facile and eco-friendly method including chemical treatment and freeze-drying. The as-prepared SHS membrane has unique wettabilities due to the hydrophilic property of the internal cellulose and the micro-nano pores, including superhydrophilicity (water contact angle of 0°) and underwater superoleophobicity (underwater oil contact angles of over 150°). The SHS membrane has good durability and stability against ultraviolet (UV) irradiation, corrosion by acids and alkalis, mechanical abrasion and especially mould adhesion. Importantly, the SHS membrane can be used for separation of various oil-in-water emulsions, and exhibits excellent separation performances such as high separation efficiency (> 99 %) and good separation flux (above 891 L m-2 h-1 bar-1). The SHS membrane also exhibits excellent recyclability over 10 continuous separation cycles. Furthermore, the SHS membrane can be utilized to selectively absorb water from oils as a water absorbent material. Hence, SHS membrane is a promising and practical material for applications in treatment of wastewater containing oil-water emulsions.

Ball-milled layer double hydroxide as persulfate activator for efficient degradation of organic: Alkaline sites-triggered non-radical mechanism.

Considerable efforts have been put into enhancing the activation performance of peroxydisulfate (PDS) by catalysts toward oxidative degradation of organic pollutants, while the oxidative selectivity is somehow overlooked. Here, we reported an enhanced non-radical oxidation pathway of PDS, activated by ball-milled Mg/Al-layered double hydroxide (BM-LDH), to reconcile the selectivity and reactivity. EPR and quenching experiments suggested that 1O2 dominated the oxidative pathway for phenol degradation without generating carcinogenic halide by-products. Multiple interfacial characterizations and density functional theory (DFT) calculations revealed that BM-LDH played dual roles in PDS activation: (1) the interlaminar BM-LDH allowed PDS intercalation to form complexed PDS, resulting in decreases in the activation barrier of PDS; (2) abundant terminal hydroxyls in the layers of BM-LDH acted as alkaline-activation sites that can efficiently activate PDS to generate 1O2 toward phenol degradation. Ball-milling treatment of LDH refined the structural hierarchy of LDH to create pore volumes, which greatly enhanced the diffusion of phenol to the intercalated PDS, resulting in more than twice the reaction rate for phenol degradation. This study provided a promising approach to simultaneously control over the reactivity and selectivity toward PDS activation that are critical for the degradation of organic pollutants particularly in drinking water treatment.

Dispersant-enhanced photodissolution of macondo crude oil: A molecular perspective.

Previous laboratory studies developed a conceptual model based on elevated non-volatile dissolved organic carbon (NVDOC) concentrations after photodegradation and subsequent dissolution of Macondo oil following the Deepwater Horizon blowout. However, those experiments did not account for the effects of ∼1 million gallons of dispersant applied to the surface oil. Here, laboratory results show photodissolution in the presence of dispersant results in > 2x increase in NVDOC concentrations after extensive photoprocessing relative to oil without dispersant. This result corresponds with an apparent increase in the percentage of surface oil photodissolution from approximately 4% in the absence of dispersant to 7% in the presence of dispersant. The oil and dissolved products were analyzed by excitation-emission matrix spectroscopy and ultrahigh resolution mass spectrometry. The compounds that persisted in the oil phase are relatively aromatic without dispersant, while those in the presence of dispersant are highly aliphatic, paraffinic, wax-like compounds. The composition of the dissolved compounds produced from both treatment types are nearly identical after 240 h of exposure to simulated sunlight. The NVDOC and chemical composition information indicate that the photodissolution of MC252 oil in the presence of dispersant is enhanced and accelerated, suggesting that the effects of dispersants should be included in mass transfer calculations from the oil to the aqueous phase.

Facile construction of multifunctional bio-aerogel for efficient separation of surfactant-stabilized oil-in-water emulsions and co-existing organic pollutant.

The deep treatment of robust oily emulsion wastewater has long been an arduous challenge. Herein, a biomass-derived PEI-TiO2@Gelatin aerogel (PEI-TiO2@GA) with honeycomb-like porous structure was fabricated. The interface wetting characteristics of PEI-TiO2@GA could be selectively switched between the superlipophilicity and superoleophobicity through the merely pre-wetting process. Combined with extraordinary structure and superwetting properties, PEI-TiO2@GA was proved to be ideal for oils absorption (17-26 g/g) and MO dye adsorption (73.549 mg/g) with high up-taking rate. Simultaneously, as-prepared PEI-TiO2@GA could realize various surfactant-stabilized oil-in-water emulsions separation simply under gravity with the separation efficiency as high as 99.25%. In addition, PEI-TiO2@GA was highly resistant toward mechanical compression (1.952 MPa), and exhibited acceptable regenerability within 5 cycles by performing solvent replacement approach. Combining with the newly developed separator and dynamic emulsion separation device, the continuous deep separation of the emulsion and the synergistic removal of co-existing pollutants can be achieved with the enhanced separation efficiency and permeation flux. Most importantly, the mechanism results show that the transition of interface wetting properties was a reversible multi-step process, and the demulsification separation of emulsion and the adsorption removal of co-existing pollutants were two independent processes. This work opens up a new avenue to customize advanced bio-aerogels for industrial effluent treatment and environmental remediation.

Cadmium and zinc isotope compositions indicate metal sources and retention mechanisms in different soil particle size fractions.

Soil particle size may significantly affect metal distribution and stable isotopic behavior. Here, two soils were separated into four particle size fractions, namely fine sand, silt, fine silt, and colloidal particles and used to determine cadmium (Cd) and zinc (Zn) concentrations and isotope compositions. Concentrations of Cd and Zn were generally enriched in the finer particles and positively correlated with the iron (Fe) and manganese (Mn) oxide contents. However, Cd concentration in the fine sand was higher than in the silt fraction due to the higher soil organic matter contents in the former particle fraction. The maximum δ114/110Cd value was found in the colloidal particles (-0.02 and 0.01‰) of both soils while the minimum was in the silt particles (-0.12 and 0.06‰). Incorporation into the mineral lattice of Fe and Mn oxides is suggested to explain the slight enrichment of heavy Cd isotopes in the colloidal fraction. The similar δ66Zn values of the four particle fractions (0.20-0.29‰ with a mean of 0.25‰) indicate similar Zn sources in different particle sizes. Metal isotopic fingerprint of different soil particle size fractions provides further insight into the underlying metal retention mechanisms within soil micro-zones and helps in tracing metal sources and biogeochemical processes.

A novel low sampling rate and cost-efficient active sampler for medium/long-term monitoring of gaseous pollutants.

Active sampling is a dependable approach for gaseous pollutants monitoring, offering high accuracy and precision that is unaffected by environmental factors such as wind and temperature in comparison to passive sampling. To measure long-term average concentrations while minimizing the use of materials, a reduced sampling rate is necessary. Thus, this study aims to develop a novel low sampling rate (down to 1 mL/min) and cost-efficient active sampler (LASP) for medium/long-term monitoring of gaseous pollutants. The LASP mainly consisted of a syringe pump, a Y-shaped fitting with two one-way valves, and a control unit for intermittent operation. Results showed that LASP can obtain a sampling rate of less than 1 mL/min and sampling rate exhibited a high level of stability. Daily average concentrations measurements for nitrogen dioxide and formaldehyde by LASP had normalized mean biases of 2.8% and 5.2%, respectively. These numbers were - 5.8% and 6.1% for weekly-average samplings. This study demonstrated applications of LASP in real outdoor (daily-average) and indoor (weekly-average) air quality measurements. It worked well with low noise levels, and without interfering with occupants' daily activities. LASP can assist in improving our ability to monitor air quality and pollutants emissions, thereby supporting health research and policy development. ENVIRONMENTAL IMPLICATION: Gaseous air pollution is an important hazardous factor threatening human health. Medium/long-term air quality monitoring is essential for outdoor and indoor air quality assessment and control. However, air sampler for medium/long-term sampling is lacking. This study developed a novel low sampling rate and cost-efficient active sampler and applied it to medium/long-term air sampling. The sampler can work at a sampling rate of less than 1 mL/min. This technology provides a feasible strategy for medium/long-term monitoring of gaseous air pollutants in both environments and emission hotspots.

Towards environmentally sustainable management: A review on the generation, degradation, and recycling of polypropylene face mask waste.

There has been a considerable increase in the use of face masks in the past years. Managing face mask waste has become a global concern, as the current waste management system is insufficient to deal with such a large quantity of solid waste. The drastic increase in quantity, along with the material's inability to degrade plastic components such as polypropylene, has led to a large accumulation of plastic waste, causing a series of environmental and ecological challenges. In addition, the growing use also imposes pressure on waste management methods such as landfill and incineration, raising concerns about high energy consumption, low value-added utilization, and the release of additional pollutants during the process. This article initially reviews the impact of mask-related plastic waste generation and degradation behavior in the natural environment. It then provides an overview of various recently developed methods for recycling face mask plastic waste. The article also offers forward-looking strategies and recommendations on face mask plastic waste management. The review aims to provide guidance on harnessing the complexities of mask waste and other medical plastic pollution issues, as well as improving the current waste management system's deficiencies and inefficiencies in tackling the growing plastic waste problem.

Mo(VI) removal from water by aluminum electrocoagulation: Cost-effectiveness analysis, main influencing factors, and proposed mechanisms.

Mo(VI) (MoO42-) removal by aluminum electrocoagulation (Al EC) with Al as anodes and cathodes was studied for the first time. At the initial Mo concentrations of 0.3 - 150 mg/L, kinetic analysis and effects of main factors (electrode connection modes, current density (CD), initial pH, and electrolytes) were examined, and potential mechanism of Mo(VI) removal were elucidated. Results showed that CD had significant impacts on anode weight loss, cathode weight loss, and total electrode weight loss (p value < 0.05). Cathode weight loss was higher than anode weight loss. XRD analysis results showed lower crystallinity of scums than that of precipitates. Boehmite was the most prevalent oxide in scums. An appropriate amount of NaCl was beneficial for enhancing the Mo(VI) removal efficiency and reducing the energy consumption of the Al EC process. Electrostatic attraction, surface complexation, hydroxyl exchange, flocculation, and coprecipitation were the main mechanisms involved in the Mo(VI) removal process by Al EC. Al EC outperformed conventional chemical coagulation in terms of Mo(VI) removal at the same dosage of Al. The Mo(VI) removal efficiencies in two real water samples (lake water and river water) reached up to 89.2% and 71.2%, respectively. This study provides novel insights into the strategies for the removal of oxoanionic metal pollutants and reduction of operating cost by Al EC technology.

A clean method for gallium recovery and the coproduction of silica-potassium compound fertilizer and zeolite F from brown corundum fly ash.

Brown corundum fly ash (BCFA) is a solid waste from the brown corundum smelting process that contains abundant Ga, K, Si, and Al, but effectively extracting Ga can be challenging since most of it is located inside the particles. This study proposes a comprehensive utilization method of BCFA that combines hydrothermal leaching and alkali regeneration to extract Ga efficiently while producing silica-potassium compound fertilizer (SPCF) and zeolite F. By utilizing the transformation of phase and structure in the hydrothermal leaching process, Ga extraction is efficiently achieved. The results showed that under the conditions of 210 g/L KOH concentration, a liquidsolid ratio of 25 mL/g, and 160 °C hydrothermal leaching for 60 min, the extraction efficiencies of Ga, K, and Si were 95.91 %, 51.78 %, and 69.57 %, respectively. The solid product's effective SiO2 and K2O contents increased to 24.72 wt% and 17.74 wt%, respectively, which can be further used as SPCF for agricultural production. The hydrothermal leaching solution was regenerated by adjusting the Al/Si molar ratio and crystalizing at 160 °C for 24 h. The Si was recovered in the form of high value-added zeolite F, with only a 3.60 % loss of Ga.

Robust mussel-inspired superhydrophobic sponge with eco-friendly photothermal effect for crude oil/seawater separation.

Frequent oil spills have significant implications for the preservation of ecological balance. However, conventional superhydrophobic materials are limited to organic solvent separation, lacking the ability to undergo thermal conversion. In response to these challenges, photothermal materials have emerged as a promising, environmentally friendly, and cost-effective solution. These materials utilize solar energy as a constant power source to effectively reduce the viscosity of crude oil without the need for additional energy input. This work presents the self-assembly of titanium nitride/polydopamine (TiN/PDA) nanoparticles using hydrolytic methyltrimethoxysilane (MTMS) on the polyurethane (PU) sponge and subsequently dip-coats this sponge with polydimethylsiloxane (PDMS). The TiN nanoparticles act as a photothermal medium, while the PDA coating exhibits a photothermal synergistic effect on TiN nanoparticles. Additionally, the PDA coating demonstrates strong adhesion on the PU sponge through chemical bonding with MTMS, as confirmed by density functional theory (DFT). Furthermore, the superhydrophobic sponges process exceptional mechanical or chemical stability in harsh environments, thanks to the dual protective mechanisms provided by MTMS and PDMS. Particularly important, the excellent photothermal conversion efficiency of this material results in a maximum temperature of 99.4 °C being achieved within 3 min and a stable heating performance of over 99.0 °C across 10 cycles under a standard sunlight intensity. These superhydrophobic sponges can be effectively utilized for continuous vacuum-assisted separation of crude oil/seawater, enabling rapid adsorption and purification in oceanic environments.

Containment of phenol-impacted groundwater by vertical cutoff wall with backfill consisting of sand and bentonite modified with hydrophobic and hydrophilic polymers.

A novel soil-bentonite backfill is proposed for use in vertical cutoff walls to contain phenol in groundwater at contaminated sites. The backfill consists of sand and bentonite modified with tetramethylammonium and carboxymethylcellulose, labeled as STCMB backfill. Flexible-wall permeability and double-reservoir diffusion tests were conducted to investigate the impact of phenol solution on hydraulic conductivity (k), effective diffusion coefficient (D*) and partition coefficient (Kp) of the backfill, respectively. The permeability results showed k of the STCMB backfill decreased by 0.91 times when the permeating liquid was changed from tap water to phenol solution. The diffusion testing results showed that D* values for the STCMB and conventional backfill (labeled as SCB backfill) were 4.0 × 10-10 m2/s and 3.0 × 10-10 m2/s, respectively, whereas Kp values for the STCMB and SCB backfills were 2.0 mL/g and 0.75 mL/g, respectively. The octanol-water partition coefficient model is suitable for estimating Kp for nonpolar organics. Furthermore, a series of solute transport simulations using Pollute V7 program was performed to evaluate the performance of vertical cutoff walls comprising STCMB and SCB backfills in containing phenol in lateral flowing groundwater. Overall, the STCMB backfill has demonstrated superior effectiveness in containing phenol in groundwater.

Plasma-catalyzed combined dynamic wave scrubbing: A novel method for highly efficient removal of multiple pollutants from flue gas at low temperatures.

In this study, we developed a novel approach combining a non-thermal plasma system with M(Ce, Cu)-Mn/13X oxidation and post-dynamic wave wet scrubbing technologies, for effectively removing multiple pollutants from flue gases. Experimental results demonstrated that the plasma coupled with post-dynamic wave wet scrubbing achieved impressive synergistic removal efficiencies of 98% for SO2, 50.9% for NO, and 51.3% for Hg0 in flue gas. Through the use of M(Ce, Cu)-Mn/13X catalysts synthesized via the co-precipitation, the oxidation efficiency of the system is significantly enhanced, with synergistic removal efficiencies reaching up to 100% for SO2, 98.7% for NO, and 96% for Hg0. Notably, (Ce-Mn)/13X exhibited superior catalytic activity, the results are supported by comprehensive sample characterization, DFT mechanistic analysis, and experimental validation. Additionally, we elucidated the plasma oxidation mechanism and the working principles of the M(Ce, Cu)-Mn/13X loaded catalysts. This innovative technology not only facilitates pollutant oxidation but also ensures their complete removal from flue gas, providing a high-efficiency, cost-effective, and environmentally friendly solution for the treatment of multi-pollutants in flue gases.

Hydrophobic organogel sorbent and its coated porous substrates for efficient oil/water emulsion separation and effective spilled oil remediation.

Frequent offshore oil leakage accidents and large quantities of oily-wastewater produced in industry and daily life bring huge challenges to global water purification. The adaptability and stability of organogels as adsorbent materials have shown wide application prospects in the field of oil-water separation. Herein, the organogels displayed stable hydrophobic/lipophilic properties with high absorption ability (1200 wt./wt%), efficient sorption of multiple emulsions (>99.0%), and good reusability. More importantly, the organogels were successfully assembled with 2D/3D substrates to achieve excellent sorption capacity (102.5 g/g) and recycling performance (50 cycles). The gel-carbon black assembled on MS (GCB-MS) sorbent with excellent photothermal conversion performance, and can rapidly heat the surface to 70.4 °C under 1.0 sunlight radiation (1.0 kW/m2) and achieved an ultra-high sorption capacity of about 103 g/g for viscous crude oil. Meanwhile, the GCB-MS was combined with a pump to build continuous oil spill cleaning equipment to achieve a super-fast cleanup rate of 6.83 g/min. The developed hydrophobic organogels had been expanded unprecedentedly to realize the comprehensive treatment of oily-wastewater in complex environments, including layered oils, emulsions, and viscous crude oil spill, which provided an effective path for the comprehensive treatment of oily wastewater in complex environments.

Development and testing of coarse-grained models for ultrasonic simulations of cast austenitic stainless steel.

Ultrasonic inspection of cast austenitic stainless steel (CASS) in the nuclear industry is particularly challenging because of sound field scatter and attenuation caused by the coarse-grained microstructure. Modeling and simulation are important tools in ultrasonic testing, as they can be used to help address key aspects of inspections, such as developing new probe designs, predicting inspection reliability, and testing phased-array focal laws. However, developing a useful and reliable CASS model is challenging due to the many grain interfaces and crystalline orientations that must be captured. We demonstrate a method of creating a realistic CASS model that is usable in CIVA, a commercially available modeling and simulation software platform. Using polished and chemically etched sections to highlight the grain structure and orientation, we generate models of a coarse-grained equiaxed specimen and a columnar specimen. We also test an alternative method of generating a coarse-grained model using Voronoi regions. We qualitatively compare sound field scatter and quantitatively compare sound field attenuation and beam partitioning in simulated sound fields to those of laboratory-measured sound fields. Results show that the Voronoi models perform as well as or better than the models based on actual grain morphology. We also show that model-to-model randomness in Voronoi grain structure can impact the magnitude of a simulated echo response by a factor of two or more. Although CASS models are potentially a good depiction of reality for a given scenario, they should not be considered representative since CASS morphology can change significantly from specimen to specimen or within the same specimen.

Quantifying uranium radio-isotope ratios in riverine suspended particulate matter: Insights into natural and anthropogenic influences in the glacial-fed river system of the NE Tibetan Plateau.

The analysis of uranium isotope ratio 235U/238U in environmental media serves as a reliable method to distinguish between natural and anthropogenic sources of uranium, playing a crucial role in assessing the extent of contamination with anthropogenic uranium and disturbances in its biogeochemical cycle. In this study, we focus on the northeastern Tibetan Plateau to examine the atomic ratio of 235U and 238U in riverine suspended particulate matter (SPM) across eight glacial watersheds. Results reveal that the 235U/238U atomic ratio in the suspended load ranges from 0.007247 to 0.007437 (with an average value of 0.00727 ± 0.00003), which closely aligns with the ratio found in natural uranium (0.00725). The highest mean ratio (0.00729 ± 0.00007) is observed in the upper glacial basin of the Ningchan River. Results suggest the negligible influence of isotopically altered in relation to human nuclear activities. When considering different environmental media, such as soil, snow/cryoconite, and riverine suspended particulate matter in the study area, the 235U/238U ratio in surface soil presents the highest values, pointing to a slight enrichment of 235U. This may be attributed to the fact that soil retains the cumulative signals of uranium atmospheric deposition, including the deposition of 235U-enriched airborne particulate matter deposited after atmospheric nuclear tests carried out in the second half of the 20th century. On the contrary, riverine suspended particulate matter and glacial sediments are more influenced by the natural 235U/238U signature under modern environmental conditions. This confirms that the northeastern Tibetan Plateau is still relatively pristine with respect to biogeochemical disturbances related to human activities.

Critical layer in liquid-solid system influencing the remediation of chromium using zeolite-supported sulfide nano zero-valent iron.

Sulfidated nano zero-valent iron particles were immobilized on ZSM-5 zeolite (Z/S-nZVI) and used for hexavalent chromium (Cr(VI)) remediation. The performance of Z/S-nZVI improved with the increase in Cr(VI) concentration (< 60 mg/L), while the performance significantly decreased for a Cr(VI) concentration of more than 60 mg/L. The adsorption behavior for Cr(VI) was different from that reported in previous studies. The improved performance can be tailored for increasing efficiency of nano zero-valent iron (nZVI) corrosion, while the degree of corrosion of nZVI was affected by the concentration of the pollutant as discussed by kinetics, X-ray diffraction (XRD) and X-ray photoelectron spectrometer (XPS) analyses. The experiments for the dissolution of ferrous ions and the dosage of adsorbent demonstrated that the critical layer in the liquid-solid system changed with the increase in the concentration of Cr(VI) (Cr(VI): Z/S-nZVI > 0.6). Moreover, the removal mechanisms of Cr(VI) were elucidated through XRD, transmission electron microscopy (TEM) and XPS techniques. This results demonstrate that the species of chromium in the critical layer changed from Cr(III) to Cr(VI) as the concentration of chromium increased from low to high. Furthermore, the critical layer was composed of Cr(VI), Fe(II), O and H elements. Additionally, the experiments of coexisting ions and aging time confirmed that Z/S-nZVI possessed high selectivity and stability to ensure efficiency and cost-effectiveness in practical applications.

Greenhouse gas emissions from Daihai Lake, China: Should eutrophication and salinity promote carbon emission dynamics?

Greenhouse gases (GHGs) emitted or absorbed by lakes are an important component of the global carbon cycle. However, few studies have focused on the GHG dynamics of eutrophic saline lakes, thus preventing a comprehensive understanding of the carbon cycle. Here, we conducted four sampling analyses using a floating chamber in Daihai Lake, a eutrophication saline lake in Inner Mongolia Autonomous Region, China, to explore its carbon dioxide (CO2) and methane (CH4) emissions. The mean CO2 emission flux (FCO2) and CH4 emission flux (FCH4) were 17.54 ± 14.54 mmol/m2/day and 0.50 ± 0.50 mmol/m2/day, respectively. The results indicated that Daihai Lake was a source of CO2 and CH4, and GHG emissions exhibited temporal variability. The mean CO2 partial pressure (pCO2) and CH4 partial pressure (pCH4) were 561.35 ± 109.59 µatm and 17.02 ± 13.45 µatm, which were supersaturated relative to the atmosphere. The regression and correlation analysis showed that the main influencing factors of pCO2 were wind speed, dissolved oxygen (DO), total nitrogen (TN) and Chlorophyll a (Chl.a), whereas the main influencing factors of pCH4 were water temperature (WT), Chl.a, nitrate nitrogen (NO3--N), TN, dissolved organic carbon (DOC) and water depth. Salinity regulated carbon mineralization and organic matter decomposition, and it was an important influencing factor of pCO2 and pCH4. Additionally, the trophic level index (TLI) significantly increased pCH4. Our study elucidated that salinity and eutrophication play an important role in the dynamic changes of GHG emissions. However, research on eutrophic saline lakes needs to be strengthened.

In situ immunomodulation of tumors with biosynthetic bacteria promote anti-tumor immunity.

Immune checkpoint blockade (ICB) therapy potently revives T cell's response to cancer. However, patients suffered with tumors that had inadequate infiltrated immune cells only receive limited therapeutic benefits from ICB therapy. Synthetic biology promotes the alternative strategy of harnessing tumor-targeting bacteria to synthesize therapeutics to modulate immunity in situ. Herein, we engineered attenuated Salmonella typhimurium VNP20009 with gene circuits to synthetize granulocyte-macrophage colony-stimulating factor (GM-CSF) and interleukin 7 (IL-7) within tumors, which recruited dendritic cells (DCs) and enhanced T cell priming to elicit anti-tumor response. The bacteria-produced GM-CSF stimulated the maturation of bone marrow-derived dendritic cells (BMDCs), while IL-7 promoted the proliferation of spleen isolated T cells and inhibited cytotoxicity T cell apoptosis in vitro. Virtually, engineered VNP20009 prefer to colonize in tumors, and inhibited tumor growth by enhancing DCs and T cell infiltration. Moreover, the tumor-toxic GZMB+ CD8+ T cell and IFN-γ+ CD8+ T cell populations conspicuously increased with the treatment of engineered bacteria. The combination of GM-CSF-IL-7-VNP20009 with PD-1 antibody synergistically stunted the tumor progress and stasis.