Cloning and functional characterization of the bile acid-sensitive methotrexate carrier from rat liver cells.
Hepatology
; 31(6): 1296-304, 2000 Jun.
Article
en En
| MEDLINE
| ID: mdl-10827155
ABSTRACT
We have cloned two complementary DNAs (cDNAs), RL-Mtx-1 and RL-Mtx-2, corresponding to the bile acid- sensitive methotrexate carrier from rat liver by direct full-length rapid amplification of cDNA ends polymerase chain reaction (RACE-PCR) using degenerated primers that were deduced from published sequences of tumor cell methotrexate transporters. When expressed in Xenopus laevis oocytes and cosM6 cells, both clones mediate methotrexate and bumetanide transport. RL-Mtx-1 consists of 2,445 bp with an open reading frame of 1,536 bp. The corresponding protein with 512 amino acids has a molecular weight of 58 kd. RL-Mtx-2 (2,654 bp) differs by an additional insert of 203 bp. This insert is located in frame at position 1,196 of the RL-Mtx-1 and contains the typical splice junction sites at the 5' and 3' end, indicating that the RL-Mtx-2 messenger RNA (mRNA) is generated by alternative splicing. The insert contains a stop codon that shortens the RL-Mtx-2 protein to 330 amino acids (38 kd). Both cDNAs contain the binding site sequence for the dioxin/nuclear translocator responsive element (Ah/Arnt-receptor) in conjunction with a barbiturate recognition sequence (Barbie box). Preliminary results show that the Barbie box acts as a negative regulatory element. The two liver cDNA clones show homologies to the published sequences of folate and the reduced folate carriers, but no homology is found to the transport systems for organic anions like the Ntcp1, oatp1, OAT-K1, and OAT1. Expression of the mRNA for the methotrexate carrier is found in liver, kidney, heart, brain, spleen, lung, and skeletal muscle, but not in the testis as revealed by Northern blot analysis. The highest abundance of the mRNA is found in the kidney.
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Colección:
01-internacional
Asunto principal:
Proteínas de Transporte de Membrana
/
Ácidos y Sales Biliares
/
Proteínas Portadoras
/
Clonación Molecular
/
Transportadoras de Casetes de Unión a ATP
/
Hígado
/
Proteínas de Neoplasias
Tipo de estudio:
Diagnostic_studies
Límite:
Animals
Idioma:
En
Revista:
Hepatology
Año:
2000
Tipo del documento:
Article
País de afiliación:
Alemania