Does xanthine oxidase contribute to the hydroxyl radical generation in ischemia and reperfusion of the cochlea?
Hear Res
; 153(1-2): 1-6, 2001 Mar.
Article
en En
| MEDLINE
| ID: mdl-11223291
ABSTRACT
We investigated the effect of a hydroxyl radical scavenger, 1,3-dimethyl-2-thiourea (dimethylthiourea), and two xanthine oxidase inhibitors, oxypurinol and allopurinol, on the threshold shift of the compound action potential (CAP) after transient ischemia of the cochlea. Transient ischemia of 30 min duration was induced in albino guinea pigs via a skull base approach. The animals were treated with perilymphatic perfusion of dimethylthiourea, oxypurinol or allopurinol from 10 min before the onset of ischemia to 4 h after the termination of ischemia. Dimethylthiourea ameliorated the CAP threshold shifts at 4 h after the onset of reperfusion in a dose-dependent manner. However, oxypurinol and allopurinol did not affect the post-ischemic cochlear dysfunction. These results imply that the hydroxyl radical plays an important role in generation of cochlear dysfunction induced by ischemia-reperfusion and that xanthine oxidase may not be the primary source of this radical.
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Colección:
01-internacional
Asunto principal:
Tiourea
/
Xantina Oxidasa
/
Daño por Reperfusión
/
Cóclea
/
Radical Hidroxilo
/
Isquemia
Límite:
Animals
Idioma:
En
Revista:
Hear Res
Año:
2001
Tipo del documento:
Article
País de afiliación:
Japón