The coxsackie adenovirus receptor inhibits cancer cell migration.
Exp Cell Res
; 298(2): 624-31, 2004 Aug 15.
Article
en En
| MEDLINE
| ID: mdl-15265708
ABSTRACT
The coxsackie and adenovirus receptor (CAR) is a key factor in adenoviral cancer gene therapy. Reduced expression of CAR during progression of prostate and bladder cancer has been reported. In embryonic development and tissue differentiation, CAR is also differentially expressed. This study suggests a role of CAR expression in cell adhesion and cell motility of human cancer cells. Stable CAR-expressing clones from E-cadherin-deficient A2780 ovarian and CaSki cervical cancer cells with originally low and high CAR expression levels, respectively, were established. CAR reexpression in otherwise singularly growing A2780 parental cells resulted in formation of cell-cell contacts and aggregation in cell clusters. CAR overexpression in cell adhesion-forming CaSki cells did not result in morphological changes. Migration of the A2780 CAR clones was strongly reduced as characterized by using spread-off assays. Using migration chambers, formation of satellite colonies was reduced by 97% in CAR-expressing A2780 cell clones and by 23% in CAR-expressing CaSki cell clones. Parental A2780 and CaSki cells selected for high migratory ability by using migration chambers expressed endogenous CAR on lower levels associated with lower adenoviral transduction efficiency. Our data suggest CAR as a new inhibitory factor for cancer cell migration.
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Colección:
01-internacional
Asunto principal:
Receptores Virales
/
Movimiento Celular
/
Metástasis de la Neoplasia
/
Neoplasias
Límite:
Humans
Idioma:
En
Revista:
Exp Cell Res
Año:
2004
Tipo del documento:
Article