Cytosolic type II estrogen binding site in rat uterus: specific photolabeling with estrone.
J Recept Res
; 12(2): 217-31, 1992.
Article
en En
| MEDLINE
| ID: mdl-1583622
ABSTRACT
A low affinity (Kd = 30 nM), large capacity (Bmax = 2.6 pmol/g tissue) estrogen binding site was photolabeled from estradiol-stimulated rat uterus cytosol. To maximize levels of this binding site and reduce those of the type I binding site, ovariectomized rats were injected with high doses of estradiol (10 micrograms per day) for four days with the last injection two hours before sacrifice. This treatment depleted type I estrogen receptors from the cytosol (by 90%) and raised levels of type II sites in the nucleus without affecting cytosolic type II levels. The type II estradiol binding sites were distinguished from the type I sites on the basis of their dissociation kinetics, pH-sensitivity and their behavior towards potassium chloride, somatostatin, sodium thiocyanate, sulfhydryl reagents and ammonium sulfate precipitation. These type II binding sites could be covalently photolabeled with tritiated estrone. A molecular weight of 43 kDa was found on SDS PAGE.
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Colección:
01-internacional
Asunto principal:
Útero
/
Citosol
/
Estrona
Límite:
Animals
Idioma:
En
Revista:
J Recept Res
Año:
1992
Tipo del documento:
Article
País de afiliación:
Francia