Biosynthesis of Cd-bound phytochelatins by Phaeodactylum tricornutum and their speciation by size-exclusion chromatography and ion-pair chromatography coupled to ICP-MS.
Anal Bioanal Chem
; 383(3): 398-403, 2005 Oct.
Article
en En
| MEDLINE
| ID: mdl-16205894
ABSTRACT
Cd-bound phytochelatins (Cd-PCs) have been synthesised by incubation of Phaeodactylum tricornutum cell cultures with Cd and purified by size-exclusion chromatography-UV-Vis. These complexes, which were identified in previous work, have now been used as model substances to develop and optimise ion-pair chromatography (IPC) coupled to inductively coupled plasma-mass spectrometry (ICP-MS) for analysis of Cd-PCs. Subsequent analysis of samples taken from Silene vulgaris plants cultivated under heavy metal stress conditions revealed Cd signals but no Cd-PC signals. By use of isotopically enriched (116)Cd-PCs the sample preparation steps were verified to determine the stability of the analytes. We observed species transformation between Cd-PCs and other unidentified Cd complexes. Consequently, the kinetic and thermodynamic lability of Cd-PCs are decisive factors in their detection.
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Colección:
01-internacional
Asunto principal:
Cadmio
/
Eucariontes
/
Glutatión
Tipo de estudio:
Prognostic_studies
Idioma:
En
Revista:
Anal Bioanal Chem
Año:
2005
Tipo del documento:
Article
País de afiliación:
Alemania