[Experimental study on acellular bovine pericardium guided bone regeneration material].

ABSTRACT

OBJECTIVE: To choose the best procedure on preparation of acellular bovine pericardium (ABP) guided bone regeneration (GBR) material. METHODS: The BP was decellularized with 0. 25% Trypsin + 0.5% Triton X-100. The acellular bovine pericardiums (ABPs) were treated with phosphate-buffered saline(PBS) (group A), 95% glycerol (group B), EDAC (group C), and EDAC and 95% glycerol (group D) respectively. The treated ABPs were implanted subcutaneously in the back of SD rats respectively at random and no material was implanted as control. Seven rats were sacrificed at 2 weeks, twelve at 4 weeks, twelve at 8 weeks, seven at 16 weeks. Local reaction was studied grossly. The amount of antigen presenting cell (APC) and the percentage of ABP degeneration were reckoned by images analysis system. RESULTS: The ABPs were replaced by fibroblasts completely in group A at 8 weeks, in group C at 16 weeks, but only less than 50% till 16 weeks in groups B and D. In all groups, the depth of surrounding fibres attenuated time-dependently. The APC amount of the groups B and D was higher than that of the control group, and the ABP of the groups B and D degraded partly at 16 weeks. CONCLUSION: The ABP treated with EDAC can be replaced by the surrounding tissues and has good biocompatibility.