[The source of the iron binding with nitric oxide in activated macrophages]. / Ob istochnike zheleza, sviazyvaiushchegosia s oksidom azota v aktivirovannykh makrofagakh.
Izv Akad Nauk SSSR Biol
; (5): 784-8, 1991.
Article
en Ru
| MEDLINE
| ID: mdl-1665500
ABSTRACT
No decrease in iron-sulphur centers was found in cultured macrophage cells (J774) after the treatment with nitric oxide (10(-7) M NO/10(7) cells) during 5 min. The center content was controlled by the electron spin resonance (ESR) method. The macrophages pretreated with dithionite + methyl viologen showed the formation of dinitrosyl iron complexes (DNIC) with a characteristic ESR signal at g approximately 2.03. The data suggest that loosely bound nonheme iron (free iron) mostly contributes to the formation of these complexes. Iron from iron-containing proteins does not release from these centers under the direct action of nitric oxide. The iron-sulphur centers can be destroyed by the products of nitric oxide oxidation (NO2, N2O3, etc.) as oxidizing and acid agents.
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Colección:
01-internacional
Asunto principal:
Hierro
/
Activación de Macrófagos
/
Macrófagos
/
Óxido Nítrico
Límite:
Animals
Idioma:
Ru
Revista:
Izv Akad Nauk SSSR Biol
Año:
1991
Tipo del documento:
Article