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Deficient expression of monoamine oxidase A in the endometrium is associated with implantation failure in women participating as recipients in oocyte donation.
Henriquez, S; Tapia, A; Quezada, M; Vargas, M; Cardenas, H; Rios, M; Salvatierra, A M; Croxatto, H; Orihuela, P; Zegers-Hochschild, F; Munroe, D J; Velasquez, L.
Afiliación
  • Henriquez S; Laboratorio de Inmunología de la Reproducción, Universidad de Santiago de Chile.
Mol Hum Reprod ; 12(12): 749-54, 2006 Dec.
Article en En | MEDLINE | ID: mdl-17020906
ABSTRACT
Successful implantation depends both on the quality of the embryo and on the endometrial receptivity. The latter depends on progesterone-induced changes in gene expression, a process that has been characterized by microarray analysis. One of the genes whose transcription appears to be enhanced during the receptive period is monoamine oxidase A (MAO-A). Our first objective was to confirm the increased expression of MAO-A in the endometrium during the receptive phase of spontaneous normal cycles using real time PCR and immunofluorescence. The second objective was to examine the endometrial expression of MAO-A during the receptive phase induced by exogenous estradiol (E(2)) and progesterone in patients whose endometrium was shown to have been either receptive or non-receptive to embryo implantation in repeated cycles of oocyte donation. Results showed that MAO-A transcript levels increased between the pre-receptive (LH+3) and receptive phase (LH+7) in all spontaneous cycles examined, with a median increase of 25-fold. Immunofluorescent labelling demonstrated MAO-A localization to the glandular and luminal epithelium with an increasing positive score between LH+3 and LH+7. Conversely, prior failure of embryo implantation was associated with a 29-fold decrease in MAO-A mRNA levels and a substantial reduction in MAO-A protein immunofluorescent label score. These results show a strong association between endometrial receptivity and MAO-A expression in the endometrial epithelium, suggesting an important role for this enzyme in normal implantation.
Asunto(s)
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Colección: 01-internacional Asunto principal: Implantación del Embrión / Donación de Oocito / Pérdida del Embrión / Endometrio / Infertilidad Femenina / Monoaminooxidasa Tipo de estudio: Risk_factors_studies Límite: Adult / Female / Humans Idioma: En Revista: Mol Hum Reprod Asunto de la revista: BIOLOGIA MOLECULAR / MEDICINA REPRODUTIVA Año: 2006 Tipo del documento: Article
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Colección: 01-internacional Asunto principal: Implantación del Embrión / Donación de Oocito / Pérdida del Embrión / Endometrio / Infertilidad Femenina / Monoaminooxidasa Tipo de estudio: Risk_factors_studies Límite: Adult / Female / Humans Idioma: En Revista: Mol Hum Reprod Asunto de la revista: BIOLOGIA MOLECULAR / MEDICINA REPRODUTIVA Año: 2006 Tipo del documento: Article