Differential immunostimulating effect of granulocyte-macrophage colony-stimulating factor (GM-CSF), granulocyte colony-stimulating factor (G-CSF) and interferon gamma (IFNgamma) after severe trauma.
Inflamm Res
; 56(1): 38-44, 2007 Jan.
Article
en En
| MEDLINE
| ID: mdl-17334669
ABSTRACT
OBJECTIVE:
Severe trauma leads to an increased vulnerability to bacterial sepsis. In the present study, we compared the immunostimulating potential of granulocyte-colony stimulating-factor (G-CSF), granulocyte-macrophage colony-stimulating factor (GM-CSF) and interferon-gamma (IFN-gamma).DESIGN:
Prospective clinical experimental study.SETTING:
University hospital intensive care unit and research facility. PATIENTS 6 patients with an Injury Severity Score (ISS) of more than 25 points.INTERVENTIONS:
Heparinized blood samples of severely injured patients and 12 healthy volunteers were incubated in vitro with 10 ng/ml GM-CSF, 10 ng/ml G-CSF or 10 ng/ml IFN-gamma for 6 h. MEASUREMENTS Flow cytometry HLA-DR expression on monocytes, B- and T-lymphocytes. ELISA LPS-induced TNFalpha and IL-10 production.RESULTS:
In all patients reduced cytokine production and HLA-DR expression on monocytes was established. After administration of GM-CSF and IFN-gamma it in vitro, the level of HLA-DR expression on monocytes and the it ex vivo TNFalpha-synthesis increased while only GM-CSF increased significantly IL-10-liberation after LPS-stimulation. However, only IFN-gamma had the capacity to enhance HLA-DR on B- and T-lymphocytes. G-CSF it in vitro had no significant effect on the measured parameter.CONCLUSIONS:
These data suggest that GM-CSF and IFN-gamma may serve to support immune functions in severely injured patients.
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Colección:
01-internacional
Asunto principal:
Heridas y Lesiones
/
Adyuvantes Inmunológicos
/
Factor Estimulante de Colonias de Granulocitos
/
Factor Estimulante de Colonias de Granulocitos y Macrófagos
/
Interferón gamma
Tipo de estudio:
Observational_studies
Límite:
Adolescent
/
Adult
/
Aged
/
Aged80
/
Female
/
Humans
/
Male
/
Middle aged
Idioma:
En
Revista:
Inflamm Res
Asunto de la revista:
ALERGIA E IMUNOLOGIA
/
PATOLOGIA
Año:
2007
Tipo del documento:
Article
País de afiliación:
Alemania