Preparation of radioiodinated simian virus 40 DNA for use in DNA - DNA reassociation kinetics experiments.
Biochim Biophys Acta
; 418(3): 257-65, 1976 Feb 05.
Article
en En
| MEDLINE
| ID: mdl-174724
ABSTRACT
A method is described for the preparation of 125I-labelled SV40 DNA. Using this method, SV40 DNA can be routinely labelled to 15 - 10(6) dpm per mug; much higher specific activities are easily obtained by minor modifications of the method. Once incorporated, the radioactive label dissociates from DNA exceedingly slowly at 4 degrees C or at 68 degrees C. Iodinated SV40 DNA is shown to be useful in the quantitation of viral nucleic acid sequences in SV40-transformed 3T3 cells by DNA - DNA reassociation kinetics.
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Colección:
01-internacional
Asunto principal:
ADN Viral
/
Virus 40 de los Simios
Idioma:
En
Revista:
Biochim Biophys Acta
Año:
1976
Tipo del documento:
Article