Gliadin regulates the NK-dendritic cell cross-talk by HLA-E surface stabilization.
J Immunol
; 179(1): 372-81, 2007 Jul 01.
Article
en En
| MEDLINE
| ID: mdl-17579058
ABSTRACT
We analyzed the autologous NK cell interaction with gliadin-presenting dendritic cells. Gliadin is the known Ag priming the celiac disease (CD) pathogenesis. We demonstrate that gliadin prevents immature dendritic cells (iDCs) elimination by NK cells. Furthermore, cooperation between human NK cells-iDCs and T cells increases IFN-gamma production of anti-gliadin immune response. Gliadin fractions were analyzed for their capability to stabilize HLA-E molecules. The alpha and omega fractions conferred the protection from NK cell lysis to iDCs and increased their HLA-E expression. Gliadin pancreatic enzyme digest and a peptide derived from gliadin alpha increased HLA-E levels on murine RMA-S/HLA-E-transfected cells. Analysis of HLA-E expression in the small intestinal mucosa of gluten-containing diet celiac patients and organ culture experiments confirmed the in vitro data.
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Colección:
01-internacional
Asunto principal:
Células Dendríticas
/
Células Asesinas Naturales
/
Antígenos de Histocompatibilidad Clase I
/
Comunicación Celular
/
Membrana Celular
/
Gliadina
/
Antígenos HLA
Idioma:
En
Revista:
J Immunol
Año:
2007
Tipo del documento:
Article
País de afiliación:
Italia