[ELISA and PCR test systems used to detect Crimean-Congo hemorrhagic fever virus].
Vopr Virusol
; 52(4): 43-6, 2007.
Article
en Ru
| MEDLINE
| ID: mdl-17722612
ABSTRACT
Studying the sensitivity and specificity of enzyme-linked immunosorbent assay (ELISA) for the indication of Crimean-Congo hemorrhagic fever (CCHF) virus antigens and those of reverse transcription and polymerase chain reaction (RT-PCR) for the detection of CCHF virus RNA, and those of a intercerebral infection method in newborn albino mice systems for the determination of viral infectious activity established that the sensitivity of ELISA was 1-2 orders of magnitude less than that of RP-PCR. The latter proved to be better in studying the sera sampled from patients with CCHF. The results of studying the samples of H. marginatum ticks, the CCHF virus vectors by ELISA and RT-PCR were similar.
Buscar en Google
Colección:
01-internacional
Asunto principal:
Técnicas para Inmunoenzimas
/
Virus de la Fiebre Hemorrágica de Crimea-Congo
/
Reacción en Cadena de la Polimerasa de Transcriptasa Inversa
/
Fiebre Hemorrágica de Crimea
/
Antígenos Virales
Tipo de estudio:
Diagnostic_studies
Límite:
Animals
/
Humans
País/Región como asunto:
Africa
/
Asia
/
Europa
Idioma:
Ru
Revista:
Vopr Virusol
Año:
2007
Tipo del documento:
Article