CD61 enriches long-term repopulating hematopoietic stem cells.
Biochem Biophys Res Commun
; 365(1): 176-82, 2008 Jan 04.
Article
en En
| MEDLINE
| ID: mdl-17983596
ABSTRACT
Among the subsets that define hematopoietic stem cells (HSCs), CD34- c-kit+ Sca-1+ lineage marker- (CD34-KSL) cells are regarded as one of the populations that have the highest enrichment of HSCs in adult mouse bone marrow. Here, we demonstrate that long-term repopulating hematopoietic stem cells (LTR-HSCs) have high expression of CD61 (integrin beta3) within the CD34-KSL population. Approximately 60% of CD34-KSL cells showed high expression of CD61. CD61HighCD34-KSL populations also exhibited significantly greater properties of HSC, such as expression of HSC markers, the side population (SP) phenotype, and ability for long-term repopulation. In both SP cells and non-SP (NSP) cells, CD61HighCD34-KSL cells also contained significantly more LTR-HSCs than CD61Low/-CD34-KSL cells. Our results indicate that CD61 is exploitable for HSC enrichment as a supportive positive cell surface marker.
Buscar en Google
Colección:
01-internacional
Asunto principal:
Células Madre Hematopoyéticas
/
Integrina beta3
Límite:
Animals
Idioma:
En
Revista:
Biochem Biophys Res Commun
Año:
2008
Tipo del documento:
Article
País de afiliación:
Japón