Development and optimization of a binding assay for histone deacetylase 4 using surface plasmon resonance.
Anal Biochem
; 377(2): 267-9, 2008 Jun 15.
Article
en En
| MEDLINE
| ID: mdl-18381195
ABSTRACT
Histone deacetylase 4 (HDAC4) is a histone deacetylase profoundly involved in cell differentiation and in the pathogenesis of cancer. The histone deacetylase inhibitors are a new, promising class of anticancer agents. The screening of molecular interactions involving determination of the affinity of drug candidates is an integral part of the drug discovery process. Here we report the development of an assay using surface plasmon resonance for the analysis of HDAC4-small molecule interactions. We describe a new cloning and purification strategy that can be used to set up surface plasmon resonance experiments with other recombinant proteins.
Texto completo:
1
Colección:
01-internacional
Asunto principal:
Resonancia por Plasmón de Superficie
/
Histona Desacetilasas
Idioma:
En
Revista:
Anal Biochem
Año:
2008
Tipo del documento:
Article
País de afiliación:
Italia