The iscS gene deficiency affects the expression of pyrimidine metabolism genes.
Biochem Biophys Res Commun
; 372(3): 407-11, 2008 Aug 01.
Article
en En
| MEDLINE
| ID: mdl-18482579
ABSTRACT
Inactivation of iscS encoding cysteine desulfurase results in a slow growth phenotype associated with the deficiency of iron-sulfur clusters, thiamine, NAD, and tRNA thionucleosides in Escherichia coli. However, the other roles of iscSin vivo are unknown. By using differential screening strategies, we identified 2 pyrimidine salvage enzymes, namely, uridine phosphorylase and cytidine deaminase, which were down-regulated in the iscS mutant. Both enzymes are positively regulated by the cAMP receptor protein (CRP). We also identified a novel protein complex, namely, YeiT-YeiA, whose expression level was decreased in the iscS mutant. The recombinant YeiT-YeiA complex exhibited NADH-dependent dihydropyrimidine dehydrogenase activity, indicating its role in pyrimidine metabolism. The presence of a CRP-binding consensus sequence on the 5'-upstream of the yeiT-YeiA gene suggests its regulation by CRP. These results provide a clue to the possible role of iscS in pyrimidine metabolism by gene regulation.
Texto completo:
1
Colección:
01-internacional
Asunto principal:
Pirimidinas
/
Liasas de Carbono-Azufre
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Regulación Bacteriana de la Expresión Génica
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Proteínas de Escherichia coli
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Escherichia coli
Tipo de estudio:
Prognostic_studies
Idioma:
En
Revista:
Biochem Biophys Res Commun
Año:
2008
Tipo del documento:
Article
País de afiliación:
Japón