Membrane ERalpha-dependent activation of PKCalpha in endometrial cancer cells by estradiol.
Steroids
; 73(11): 1110-22, 2008 Oct.
Article
en En
| MEDLINE
| ID: mdl-18534651
ABSTRACT
The purpose of this study was to investigate the role of the oestrogen receptor subtypes ERalpha and ERbeta in mediating the non-genomic effects of 17-beta-estradiol (E(2)) in two human endometrial cancer cell lines (RL95-2 and HEC-1A) expressing different levels of these receptor subtypes. Western blotting analysis using phosphorylation site-specific antibodies showed that physiological concentrations of E(2) rapidly (<20 min) activated PKCalpha, but not PKCdelta in the RL95-2 cell line. E(2) had no effect on PKCalpha or PKCdelta activity in the HEC-1A cell line and suppressed basal levels of PKA activity in both cell lines. PKCalpha activation coincided with its membrane translocation. ERalpha was detected in the RL95-2 cell line by Western blotting and RT-PCR but not in the HEC-1A cells, which did express ERbeta. A selective ERalpha agonist PPT had the same effect as E(2) on PKCalpha activation in the RL95-2 cells, but the selective ERbeta agonist DPN had no such effect. A 46kDa variant of ERalpha increased in abundance in the cell membrane within 20 min of E(2) treatment suggesting that ERalpha mediated the E(2) non-genomic effects on PKCalpha through the formation of a membrane associated signalling complex.
Texto completo:
1
Colección:
01-internacional
Asunto principal:
Membrana Celular
/
Neoplasias Endometriales
/
Receptor alfa de Estrógeno
/
Estradiol
/
Proteína Quinasa C-alfa
Límite:
Female
/
Humans
Idioma:
En
Revista:
Steroids
Año:
2008
Tipo del documento:
Article