[Anti-estrogenic effect of dioxin on rat skeleton development].

ABSTRACT

OBJECTIVE: To investigate the teratogenic and anti-estrogenic effect of environment teratogen on rat skeleton development. METHODS: The fetal rat models with congenital skeleton malformation were constructed with 2, 3, 7,8-tetrachlorodibenzo-p-dioxin (TCDD) on pregnant day 10. The transparent skeleton of fetal rat was made with alizarin bordeaux. The cell pathology of chondrocyte in fetal rat center of ossification was observed by light microscope. The estradiol in rat serum was measured by radioimmunity method. RESULTS: Treated with 5-15 microg/kg TCDD, the single or multiple rat fetal skeletal development malformation had been induced. The teratogenic effect of TCDD on the osteogenesis was in dose-dependent manner. There were decreased center of ossification and cell degeneration of chondrocyte in fetal extremity limbs. The estradiol increased significantly in TCDD-treated rat, compared with normal rat (P < 0.05). CONCLUSION: TCDD can induce congenital fetal skeletal malformation under interfering the biological activity of estrogen and the function of chondrocyte and the primary center of ossification.