[Study of molecular mechanism of tanshinone II A inducing differentiation in acute promyelocytic leukemia NB4 cells].
Zhongguo Zhong Yao Za Zhi
; 33(24): 2954-8, 2008 Dec.
Article
en Zh
| MEDLINE
| ID: mdl-19294860
ABSTRACT
OBJECTIVE:
To investigate molecular mechanism of tanshinone II A inducing differentiation and apoptosis in acute promyelocytic leukemia NB4 cells.METHOD:
NB4 cells were cultured in vitro and treated with tanshinone II A and observed cellular morphology, cell category and the cellular proliferation. DNA microarray technique was used to analyze the gene expression profiles of NB4 cells induced by tanshinone II A.RESULT:
92.8% of NB4 cells treated with 0.5 mg x L(-1) tanshinone II A were induced into mature neutrophils, in which myetocytes and melamyetocytes were 27.0%, banded and segmented neutrophits 68.2%. Cell growth were inhibited. cDNA microarray showed the enormously expressed 183 genes including 23 differentiation associated genes, and other interrelated genes.CONCLUSION:
Tanshinone II A inducing differentiation in NB4 cells may be via regulation of many kinds of genes, especially differentiation associated genes expression. This partially explained the molecular mechanism of tanshinone II A inducing differentiation.
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Colección:
01-internacional
Asunto principal:
Fenantrenos
/
Medicamentos Herbarios Chinos
/
Leucemia Promielocítica Aguda
/
Diferenciación Celular
Límite:
Humans
Idioma:
Zh
Revista:
Zhongguo Zhong Yao Za Zhi
Asunto de la revista:
FARMACOLOGIA
/
TERAPIAS COMPLEMENTARES
Año:
2008
Tipo del documento:
Article
País de afiliación:
China