Surgical method to create vocal fold injuries in mice.
Ann Otol Rhinol Laryngol
; 118(2): 131-8, 2009 Feb.
Article
en En
| MEDLINE
| ID: mdl-19326764
ABSTRACT
OBJECTIVES:
The goal of this study was to develop a surgical method for the creation of vocal fold injuries in mice, as a precursor to the use of genetically engineered mouse models in the study of vocal fold wound healing and scar formation.METHODS:
Seven FVB strain mice were used in this study. A laryngoscope and 3 micro-instruments were designed and fabricated to facilitate endoscopic vocal fold visualization and the creation of vocal fold surgical injuries. The larynges were harvested 1 and 7 days after surgery, and the vocal fold injury sites were evaluated by routine hematoxylin and eosin staining. Additional immunohistochemical analysis of collagen type I and elastin distribution in the lamina propria was performed for an uninjured control larynx.RESULTS:
Endoscopic visualization and vocal fold stripping resulting in thyroarytenoid muscle exposure were successful in all animals. Histologic and immunohistochemical analyses revealed a simple lamina propria structure with relatively even collagen type I and elastin distribution in the control vocal fold, obliteration of vocal fold mucosa 1 day after surgery, and complete reepithelialization by 7 days.CONCLUSIONS:
These results demonstrate the feasibility of creating reproducible vocal fold injuries via an endoscopic approach in mice. The observation that the mouse lamina propria may have a relatively simple histologic structure indicates that additional characterization should be performed and caution used in translating findings between this and other model systems.
Texto completo:
1
Colección:
01-internacional
Asunto principal:
Pliegues Vocales
/
Cicatrización de Heridas
/
Enfermedades de la Laringe
/
Cicatriz
Tipo de estudio:
Observational_studies
/
Prognostic_studies
Límite:
Animals
Idioma:
En
Revista:
Ann Otol Rhinol Laryngol
Año:
2009
Tipo del documento:
Article
País de afiliación:
Estados Unidos