A chemical approach to immunoprotein engineering: chemoselective functionalization of thioester proteins in their native state.
Chembiochem
; 10(8): 1340-3, 2009 May 25.
Article
en En
| MEDLINE
| ID: mdl-19402090
ABSTRACT
Less than 6 feet under Serum proteins C3, C4, and alpha(2)M each contain a thioester domain buried within a hydrophobic pocket, which is thought to shield the labile thioester from hydrolysis. Herein, we make use of the inherent reactivity of the hydrazide for thioester moieties to chemoselectively label these crucial serum regulators in their native conformation; this demonstrates that access to the thioester site is much greater than previously supposed.
Texto completo:
1
Colección:
01-internacional
Asunto principal:
Compuestos de Sulfhidrilo
/
Alfa-Macroglobulinas
/
Complemento C3
/
Complemento C4b
Idioma:
En
Revista:
Chembiochem
Asunto de la revista:
BIOQUIMICA
Año:
2009
Tipo del documento:
Article
País de afiliación:
Reino Unido