Cross-linking enhances deposition of human endothelial progenitor cells in the rat heart after intracoronary transplantation.
Cell Transplant
; 19(1): 113-7, 2010.
Article
en En
| MEDLINE
| ID: mdl-19785936
ABSTRACT
Transplantation of human endothelial progenitor cells (hEPCs) may improve vascularization and left ventricular function after myocardial infarction. The scope of this study was to explore, whether cross-linking of EPCs may enhance the deposition of cells in the rat heart after clinical-like, intracoronary transplantation. To this end, (111)In-oxinate-labeled hEPCs were infused by a minimally invasive technique into the coronary arteries of immunosuppressed Wistar rats under control conditions and after ischemia/reperfusion. In a second set of experiments hEPCs were treated with phytohemagglutinin to create small cell clusters prior to transplantation. Continous three-dimensional HiSPECT images for 1 h and after 48 h revealed that cell deposition was significantly higher when hEPCs were cross-linked. Therefore, cross-linking of hEPCs may provide a promising approach to enhance the number of trapped cells also in a clinical setting.
Texto completo:
1
Colección:
01-internacional
Asunto principal:
Células Madre
/
Neovascularización Fisiológica
/
Vasos Coronarios
/
Trasplante de Células Madre
/
Células Endoteliales
Límite:
Animals
/
Humans
/
Male
Idioma:
En
Revista:
Cell Transplant
Asunto de la revista:
TRANSPLANTE
Año:
2010
Tipo del documento:
Article
País de afiliación:
Alemania